Development of an in vivo methylation system for the solventogen Clostridium saccharobutylicum NCP 262 and analysis of two endonuclease mutants.

abstract：:Streptomyces globisporus C-1027 produces a nine-membered enediyne antitumor antibiotic lidamycin. Here we report the complete genome sequence of S. globisporus C-1027, which consists of a 7,608,611 bp linear chromosome, a 167,754 bp linear plasmid SGLP1 and a 7,234 bp circular plasmid pSGL1. The biosynthetic gene clus...

journal_title：Journal of biotechnology

authors： Li X,Lei X,Zhang C,Jiang Z,Shi Y,Wang S,Wang L,Hong B

更新日期：2016-03-20 00:00:00

abstract：:Environmental biotechnology, until now, has primarily focused on the development of technologies to treat aqueous, solid and gaseous wastes. At present, the basic knowledge on how biotechnology can handle these wastes has been acquired and the focus is now on the implementation of these processes as 'best available te...

journal_title：Journal of biotechnology

authors： Grommen R,Verstraete W

更新日期：2002-09-11 00:00:00

abstract：:The pharmaceutical production of recombinant proteins, such as monoclonal antibodies, is rather complex and requires proper development work. Accordingly, it is essential to develop appropriate scale-down models, which can mimic the corresponding production scale. In this work, we investigated the impact of the biorea...

journal_title：Journal of biotechnology

authors： Bertrand V,Vogg S,Villiger TK,Stettler M,Broly H,Soos M,Morbidelli M

更新日期：2018-08-10 00:00:00

abstract：:The extraction of 13-hydroperoxide-lyase activity from mint leaves as well as its use for C6-aldehyde production was studied in this work. The enzyme cleaves 13(S)-hydroperoxy-C18 fatty acids into C6-aldehyde and C12-oxo-acid. Two mint species were tested: Mentha veridis and Mentha pulegium. The headspace injection me...

journal_title：Journal of biotechnology

authors： Gargouri M,Drouet P,Legoy MD

更新日期：2004-07-01 00:00:00

abstract：:CRISPR-Cpf1 is a type V CRISPR system that has recently been exploited for genome engineering purposes. Compared to the well-known Streptococcus pyogenes CRISPR-Cas9 system, the effector protein Cpf1 recognizes T-rich protospacer-adjacent motif (PAM) instead of G-rich PAM (used by CRISPR-Cas9), which could offer a sub...

journal_title：Journal of biotechnology

authors： Zhang J,Hong W,Zong W,Wang P,Wang Y

更新日期：2018-10-20 00:00:00

abstract：:An enzymatically-active fungal cellobiohydrolase I (CBH I) was first synthesized in a coupled reticulocyte lysate system lacking of glycosylation modification by the template DNA(Cbh1) in the presence of T7 RNA polymerase. The synthesized CBH I had the expected size (57 kDa) and catalyzed the substrate of p-nitropheny...

journal_title：Journal of biotechnology

authors： Wang J,Gao P

更新日期：2000-08-25 00:00:00

abstract：:The efficient separation of Corynebacterium glutamicum cells from culture medium by size exclusion chromatography (SEC) is presented. Residue analysis demonstrated that this method effectively depletes extracellular compounds. For evaluation, SEC was compared with the common methods cold methanol treatment, fast centr...

journal_title：Journal of biotechnology

authors： Persicke M,Plassmeier J,Neuweger H,Rückert C,Pühler A,Kalinowski J

更新日期：2011-07-10 00:00:00

abstract：:Oxygen is a key substrate in animal cell metabolism. It has been reported that the oxygen uptake rate (OUR) is a good indicator of cellular activity, and even under some conditions, a good indicator of the number of viable cells. The measurement of OUR is difficult due to many different reasons. In particular, the ver...

journal_title：Journal of biotechnology

authors： Ruffieux PA,von Stockar U,Marison IW

更新日期：1998-08-12 00:00:00

abstract：:Pseudomonas alcaligenes secretes a lipase with a high pH optimum, which has interesting properties for application in detergents. The expression of the lipase is strongly dependent on the presence of lipids in the growth medium such as soybean oil. The promoter of the gene was characterized and found to have resemblan...

journal_title：Journal of biotechnology

authors： Cox M,Gerritse G,Dankmeyer L,Quax WJ

更新日期：2001-03-09 00:00:00

abstract：:Late-embryogenesis abundant (LEA) proteins are a family of hydrophilic proteins that form an integral part of desiccation tolerance of seeds. LEA proteins have been also postulated to play a protective role under different abiotic stresses. Their role in abiotic stress tolerance has been well documented for Group 1, 2...

journal_title：Journal of biotechnology

authors： Dalal M,Tayal D,Chinnusamy V,Bansal KC

更新日期：2009-01-15 00:00:00

abstract：:The growth inhibition of a murine hybridoma mediated by ammonium chloride was shown to vary with the pH of the culture medium. Values for the initial media concentration causing 50% growth inhibition (IC50) ranged from 4 mM to 7.6 mM as the pH was reduced from 7.8 to 6.8. A significant negative correlation was observe...

journal_title：Journal of biotechnology

authors： Doyle C,Butler M

更新日期：1990-07-01 00:00:00

abstract：:One of the main limitations in bacterial polyhydroxyalkanoate (PHA) production with mixed cultures is the fact that primarily polyhydroxybutyrate (PHB) homopolymers are generated from acetate as the main carbon source, which is brittle and quite fragile. The incorporation of different 3-hydroxyalkanoate (HA) component...

journal_title：Journal of biotechnology

authors： Dai Y,Yuan Z,Jack K,Keller J

更新日期：2007-05-01 00:00:00

abstract：:The use of a proper signal sequence is one of the major determinants for the efficient secretion of heterologous proteins from yeast. The signal sequence derived from inulinase (INU1A) of Kluyveromyces marxianus was evaluated in directing the secretion of a human glycoprotein, alpha 1-antitrypsin (alpha 1-AT), from Sa...

journal_title：Journal of biotechnology

authors： Kang HA,Nam SW,Kwon KS,Chung BH,Yu MH

更新日期：1996-07-18 00:00:00

abstract：:Bifidobacterium catenulatum JCM 1194(T) was isolated from human feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. catenulatum strain. ...

journal_title：Journal of biotechnology

authors： Morita H,Toh H,Oshima K,Nakano A,Yamashita N,Iioka E,Arakawa K,Suda W,Honda K,Hattori M

更新日期：2015-09-20 00:00:00

abstract：:Many recent epigenetic studies utilize the advantages of CRISPR/dCas9 based tools in linking certain epigenetic modification with gene expression regulation. Various multifactorial diseases often contain changed epigenetic signatures at many loci, so tools for simultaneously targeting different loci would significantl...

journal_title：Journal of biotechnology

authors： Josipović G,Zoldoš V,Vojta A

更新日期：2019-08-10 00:00:00

abstract：:A general strategy to identify and quantify sample molecules in dilute solution employing a new spectroscopic method for data registration and specific burst analysis denoted as multi-parameter fluorescence detection (MFD) was recently developed. While keeping the experimental advantage of monitoring single molecules ...

journal_title：Journal of biotechnology

authors： Eggeling C,Berger S,Brand L,Fries JR,Schaffer J,Volkmer A,Seidel CA

更新日期：2001-04-13 00:00:00

abstract：:Following its introduction in 1967, rifampicin has become a mainstay of therapy in the treatment of tuberculosis, leprosy and many other widespread diseases. Its potent antibacterial activity is due to specific inhibition of bacterial RNA polymerase. However, resistance to rifampicin was reported shortly after its int...

journal_title：Journal of biotechnology

authors： Alifano P,Palumbo C,Pasanisi D,Talà A

更新日期：2015-05-20 00:00:00

abstract：:In concern with frequent recurrence of anthrax in endemic areas and inadvertent use of its spores as biological weapon, the development of an effective anthrax vaccine suitable for both human and veterinary needs is highly desirable. A simple oral delivery through expression in plant system could offer promising alter...

journal_title：Journal of biotechnology

authors： Gorantala J,Grover S,Rahi A,Chaudhary P,Rajwanshi R,Sarin NB,Bhatnagar R

更新日期：2014-04-20 00:00:00

abstract：:The feed profile of glucose during fedbatch cultivation could be used to influence the retention of the periplasmic product ZZ-cutinase. An increased feed rate led to a higher production rate but also to an increased specific leakage, which reduced the periplasmic retention. Three growth rates: 0.3, 0.2 and 0.1 h(-1) ...

journal_title：Journal of biotechnology

authors： Bäcklund E,Reeks D,Markland K,Weir N,Bowering L,Larsson G

更新日期：2008-07-31 00:00:00

abstract：:Yarrowia lipolytica can accumulate large amounts of storage lipids and has considerable potential for the production of polyunsaturated fatty acids and other lipids for biofuels. When the nitrogen source is exhausted in the medium, the key intermediate, citrate, is converted to acetyl-CoA by ATP:citrate lyase (ACL) fo...

journal_title：Journal of biotechnology

authors： Zhang H,Zhang L,Chen H,Chen YQ,Chen W,Song Y,Ratledge C

更新日期：2014-12-20 00:00:00

abstract：:Site selective chemical modification is a preferred method, employed to prolong the circulation half-life of biopharmaceuticals. Cysteines have been used as attachment point for such modification, however, to be susceptible for chemical modification the involved thiol must be in its reduced form. Proteins often contai...

journal_title：Journal of biotechnology

authors： Jespersen GR,Matthiesen F,Pedersen AK,Andersen HS,Kirsebom H,Nielsen AL

更新日期：2014-03-10 00:00:00

abstract：:Recently, some research in the field of enhanced biological phosphorus removal (EBPR) has been focused on studying systems where the electron donor (substrate) and the electron acceptor (nitrate or oxygen) are present simultaneously. This can occur, for example, in a full scale wastewater treatment plant during heavy ...

journal_title：Journal of biotechnology

authors： Pijuan M,Guisasola A,Baeza JA,Carrera J,Casas C,Lafuente J

更新日期：2006-05-03 00:00:00

abstract：:There are many examples of enzymes that share substrates or cofactors in a cyclic manner. Techniques have been developed that use cyclic enzyme systems to assay quantitatively small amounts of biochemical substances (cofactor, substrate), however, only a few studies of the control of these systems have been published....

journal_title：Journal of biotechnology

authors： Okamoto M

更新日期：1992-06-01 00:00:00

abstract：:The configuration and performance of the Biorack facility during the Second International Microgravity Laboratory mission (IML-2; 8-23 July 1995) is described in detail. During this Spacelab mission, Biorack flew with two incubators (22 degrees C and 37 degrees C), glovebox, cooler (5 degrees C) and four passive ther...

journal_title：Journal of biotechnology

authors： Manieri P,Brinckmann E,Brillouet C

更新日期：1996-06-27 00:00:00

abstract：:Formation of disulfide bonds between heavy and light chains is challenge for production of recombinant immunoglobulin G (IgG) and fragment antigen-binding (Fab). Insufficient formation of the bond influences productive yield and quality of recombinant IgG and Fab. To investigate how amino acid sequences of the first h...

journal_title：Journal of biotechnology

authors： Yusakul G,Sakamoto S,Tanaka H,Morimoto S

更新日期：2018-12-20 00:00:00

abstract：:A novel bacterial species was isolated from an industrial-scale biogas plant. The isolate Herbinix hemicellulosilytica T3/55(T) is able to degrade crystalline cellulose. Comparative 16S rRNA gene sequencing demonstrated that the isolate is closely related to environmental samples forming a hitherto unknown sub-cluster...

journal_title：Journal of biotechnology

authors： Koeck DE,Maus I,Wibberg D,Winkler A,Zverlov VV,Liebl W,Pühler A,Schwarz WH,Schlüter A

更新日期：2015-11-20 00:00:00

abstract：:GeneTag is a novel expression profiling method that allows the visualization, quantification and identification of expressed genes-whether known or novel-in any species, tissue or cell type, independent of knowledge of the underlying sequence. Here we describe the application of this method to determine variation of g...

journal_title：Journal of biotechnology

authors： Wong LY,Hafeman A,Boyd VL,Bodeau J,Lazaruk KD,Liew SN,Casey P,Belonogoff V,Bit S,Sumner C,Bredo A,Ho N,Chu E,Olson S,Rabkin S,Maltchenko S,Spier G,Gilbert D,Baumhueter S

更新日期：2003-03-20 00:00:00

abstract：:The importance of RNA-based regulation is becoming more and more evident. Genome-wide sequencing efforts have shown that the majority of the DNA in eukaryotic genomes is transcribed. Advanced high-throughput techniques like CLIP for the genome-wide detection of RNA-protein interactions have shown that post-transcripti...

journal_title：Journal of biotechnology

authors： Backofen R,Engelhardt J,Erxleben A,Fallmann J,Grüning B,Ohler U,Rajewsky N,Stadler PF

更新日期：2017-11-10 00:00:00

abstract：:Cannabinoids are secondary natural products from the plant Cannabis sativaL. Therapeutic indications of cannabinoids currently comprise a significant area of medicinal research. We have expressed the Δ9-tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) recombinantly in Komagataella p...

journal_title：Journal of biotechnology

authors： Zirpel B,Kayser O,Stehle F

更新日期：2018-10-20 00:00:00

abstract：:Fluorescence resonance energy transfer studies allow to determine global shape properties of nucleic acids and nucleoprotein complexes. In many DNA-protein complexes, the DNA is more or less bent and the degree of bending can be obtained by FRET. For example, the DNA in complex with the integration host factor (IHF) i...

journal_title：Journal of biotechnology

authors： Lorenz M,Hillisch A,Diekmann S

更新日期：2002-01-01 00:00:00