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Chemistry and applications of oligonucleotide analogues.

Abstract：

:This review is aimed at biochemists and molecular biologists, and covers the chemistry and key features involved in the solid-phase synthesis of a variety of the better known DNA and RNA analogues by the phosphoramidite and H-phosphonate methods. A wide spectrum of biological applications such as inhibition of gene expression, translation arrest, RNA processing, affinity purification of RNA-protein complexes, in situ hybridization, and synthetic ribozymes are then discussed in some detail, enabling the molecular biologist to get an idea of what is possible using the current technology.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Sproat BS

doi

10.1016/0168-1656(95)00012-f

subject

Has Abstract

pub_date

1995-07-31 00:00:00

pages

221-38

issue

2-3

eissn

0168-1656

issn

1873-4863

pii

0168-1656(95)00012-F

journal_volume

41

pub_type

杂志文章,评审

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