Complete genome sequence of Streptomyces globisporus C-1027, the producer of an enediyne antibiotic lidamycin.


:Streptomyces globisporus C-1027 produces a nine-membered enediyne antitumor antibiotic lidamycin. Here we report the complete genome sequence of S. globisporus C-1027, which consists of a 7,608,611 bp linear chromosome, a 167,754 bp linear plasmid SGLP1 and a 7,234 bp circular plasmid pSGL1. The biosynthetic gene cluster for lidamycin was located in the linear plasmid SGLP1. Genome analysis also revealed a number of genes related to biosynthesis of diverse secondary metabolites. The genome sequence of C-1027 will enable us to disclose biosynthetic pathways of these secondary metabolites and discover new natural products with potential applications notably in human health.


J Biotechnol


Journal of biotechnology


Li X,Lei X,Zhang C,Jiang Z,Shi Y,Wang S,Wang L,Hong B




Has Abstract


2016-03-20 00:00:00












  • The combination of a synthetic promoter and a CMV promoter improves foreign gene expression efficiency in myocytes.

    abstract::Skeletal muscle is becoming an attractive target tissue for gene therapy. Nevertheless, the low level of gene therapeutic expression in this tissue is the major limitation to it becoming an ideal target for gene transfer. The promoter is important element for gene transcription; however, the gene expression efficienci...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Jianwei D,Qianqian Z,Songcai L,Mingjun Z,Xiaohui R,Linlin H,Qingyan J,Yongliang Z

    更新日期:2012-04-15 00:00:00

  • Multi-assignment clustering: Machine learning from a biological perspective.

    abstract::A common approach for analyzing large-scale molecular data is to cluster objects sharing similar characteristics. This assumes that genes with highly similar expression profiles are likely participating in a common molecular process. Biological systems are extremely complex and challenging to understand, with proteins...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ulfenborg B,Karlsson A,Riveiro M,Andersson CX,Sartipy P,Synnergren J

    更新日期:2021-01-20 00:00:00

  • A reagentless amperometric biosensor for alcohol detection in column liquid chromatography based on co-immobilized peroxidase and alcohol oxidase in carbon paste.

    abstract::A reagentless carbon paste electrode chemically modified with covalently bound alcohol oxidase and horse-radish peroxidase was examined as a selective sensor in flow injection and column liquid chromatography. A combination of carbodiimide, glutaraldehyde, and polyethyleneimine was used for immobilizing the enzymes in...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Johansson K,Jönsson-Pettersson G,Gorton L,Marko-Varga G,Csöregi E

    更新日期:1993-12-01 00:00:00

  • Efficiency of cell-free protein synthesis based on a crude cell extract from Escherichia coli, wheat germ, and rabbit reticulocytes.

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    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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  • Fractionation of sulphite spent liquor for biochemical processing using ion exchange resins.

    abstract::Sulphite spent liquor (SSL) is a side product from acidic sulphite pulping of wood, which organic counterpart is composed mainly by lignosulphonates (LS) and sugars. The last are a prominent substrate for the bioprocessing although a previous purification step is necessary to eliminate microbial inhibitors. In this st...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Fernandes DL,Silva CM,Xavier AM,Evtuguin DV

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  • Plasmid instability kinetics in continuous culture of a recombinant Saccharomyces cerevisiae in airlift bioreactor.

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    pub_type: 杂志文章


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  • Large double copy vectors are functional but show a size-dependent decline in transduction efficiency.

    abstract::The development of SIN-lentivectors has paved the way for the double copy vectors (DCV), which substitute the deletion in the 3'LTR with either transgenic or insulator sequences. However, the limits of this approach remain unclear. Previous results have demonstrated that transduction efficiencies of DCV carrying large...

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    pub_type: 杂志文章


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  • Site-specific tetrameric streptavidin-protein conjugation using sortase A.

    abstract::Streptavidin is tetrameric protein which has tight and specific biotin binding affinity, and streptavidin modification of proteins or small molecules is widely used for biotechnology tool. Here, we demonstrate site-specific streptavidin-protein conjugation using enzymes. We focused on sortase A, a transpeptidase from ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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  • The heparanome--the enigma of encoding and decoding heparan sulfate sulfation.

    abstract::Heparan sulfate (HS) is a cell surface carbohydrate polymer modified with sulfate moieties whose highly ordered composition is central to directing specific cell signaling events. The ability of the cell to generate these information rich glycans with such specificity has opened up a new field of "heparanomics" which ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


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  • Preparation of a reversible soluble-insoluble β-d-Glucosidase with perfect stability and activity.

    abstract::Rebuilding enzymes with high activities, stability and reusability has been constantly pursued for application of enzymaticcatalysis in wide field. Rebuilding enzymes by chemical approaches, including immobilization and chemicalmodification, represents a simple but effective route. Here, a class of highly stable and a...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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    pub_type: 杂志文章


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  • Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley).

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yu CY,Li XF,Lou WY,Zong MH

    更新日期:2013-06-20 00:00:00

  • Molecular recognition of esterase plays a major role on the removal of fatty soils during detergency.

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    pub_type: 杂志文章


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    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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  • Enoate reductases from non conventional yeasts: bioconversion, cloning, and functional expression in Saccharomyces cerevisiae.

    abstract::Old yellow enzymes (OYEs, EC are flavin-dependent oxidoreductases that catalyze the stereoselective trans-hydrogenation of the double bond, representing a promising alternative to metal-based catalysis. Bioconversion of ketoisophorone (KIP) by 28 non-conventional yeasts belonging to 16 different species was ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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    更新日期:2011-12-20 00:00:00

  • Improved fermentative production of the compatible solute ectoine by Corynebacterium glutamicum from glucose and alternative carbon sources.

    abstract::The cyclic amino acid ectoine is a compatible solute serving as a protective substance against osmotic stress. Ectoine finds various applications due to its moisturizing effect. To avoid the disadvantages of the prevailing so-called "bacterial milking ectoine production process" caused by the high salt concentration, ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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    更新日期:2017-09-20 00:00:00

  • Expression of hepatitis B surface antigen S domain in recombinant Saccharomyces cerevisiae using GAL1 promoter.

    abstract::Hepatitis B virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. The S domain of hepatitis B virus surface antigen (sHBsAg) was expressed under the control of the galactose-inducible GAL1 promoter in recombinant Saccharomyces cerevisiae. Batch fermentation of S. cerevis...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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    更新日期:2009-05-20 00:00:00

  • Bioelectrocatalysis: the electrochemical kinetics of hydrogenase action.

    abstract::A hydrogen electrode based on a hydrogenase immobilized on a carbon material has been developed. The equilibrium hydrogen potential was reached in an atmosphere of H2 on the enzyme electrode according to the mechanism of direct exchange of electrons between the electrode and the enzyme active center. The electrochemic...

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    pub_type: 杂志文章


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  • 'Structural constraints in cyanobacteria-mediated whole-cell biotransformation of methoxylated and methylated derivatives of 2'-hydroxychalcone.

    abstract::Halophilic and freshwater strains of cyanobacteria representing the Oscillatoriales, Nostocales, Chroococcales, and Synechococcales orders of Cyanophyta were examined to determine (i) the resistance of their cultures when suppressed by the presence of exogenous methoxylated and methylated derivatives of 2'-hydroxychal...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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  • Transcript stabilization by mRNA sequences from hrpA of Pseudomonas syringae.

    abstract::Production of heterologous proteins in bacteria is one of the main applications of biotechnology. Although several high-efficiency expression systems have been developed, different steps in protein production may become rate-limiting depending on the production system and the protein being produced. One bottle neck ca...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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    更新日期:2007-02-01 00:00:00

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    abstract::The genome sequence of Corynebacterium glutamicum, a gram-positive soil bacterium widely used as an amino acid producer, was analyzed by a similarity-based approach to elucidate the pathway for the biosynthesis of L-methionine. The functions of candidate ORFs were derived by gene deletion and, if necessary, by homolog...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Rückert C,Pühler A,Kalinowski J

    更新日期:2003-09-04 00:00:00

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    abstract::It has been well recognized that many key aspects of cell cycle regulation are encoded into the size distributions of growing budding yeast populations due to the tight coupling between cell growth and cell division present in this organism. Several attempts have been made to model the cell size distribution of growin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hatzis C,Porro D

    更新日期:2006-07-13 00:00:00

  • Assembly of bacteriophage Qbeta virus-like particles in yeast Saccharomyces cerevisiae and Pichia pastoris.

    abstract::Recombinant bacteriophage Qbeta coat protein (CP), which has been proposed as a promising carrier of foreign epitopes via their incorporation either by gene engineering techniques or by chemical coupling, efficiently self-assembles into virus-like particles (VLPs) when expressed in Escherichia coli. Here, we demonstra...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Freivalds J,Dislers A,Ose V,Skrastina D,Cielens I,Pumpens P,Sasnauskas K,Kazaks A

    更新日期:2006-05-29 00:00:00

  • A flow cytometric study of hydrodynamic damage to mammalian cells.

    abstract::Flow cytometry has been used to study the mechanisms of damage to mammalian cells by hydrodynamic forces. Cell damage resulted from the stresses created both by bubble entrainment and by bubble bursting caused by vortex formation in highly agitated culture. Damage to the antigen molecules on the cell surface together ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


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  • Coupled production in biorefineries--combined use of biomass as a source of energy, fuels and materials.

    abstract::In spite of high prices for fossil raw materials the production of biomass-based products is rarely economically successful today. Depending on the location feedstock prices are currently so high that products from renewable resources are not marketable when produced in existing process chains. Apart from the higher f...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lyko H,Deerberg G,Weidner E

    更新日期:2009-06-01 00:00:00

  • Thermal stable and oxidation-resistant variant of subtilisin E.

    abstract::A remarkable thermal stable and oxidation-resistant mutant was obtained using the random mutagenesis PCR technique on the mutant M222A gene of subtilisin E. Sequencing analysis revealed an A was replaced by G at nucleotide 671 of the subtilisin E gene, converting the asparagine codon (AAT) to serine codon (AGT) at pos...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yang Y,Jiang L,Zhu L,Wu Y,Yang S

    更新日期:2000-08-25 00:00:00

  • Integration of distributed multi-analyzer monitoring and control in bioprocessing based on a real-time expert system.

    abstract::A computer system solution for integration of a distributed bioreactor monitoring and control instrumentation on the laboratory scale is described. Bioreactors equipped with on-line analyzers for mass spectrometry, near-infrared spectroscopy, electrochemical probes and multi-array gas sensors and their respective soft...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Cimander C,Bachinger T,Mandenius CF

    更新日期:2003-08-15 00:00:00

  • Combinatorial biosynthesis--potential and problems.

    abstract::Because of their ecological functions, natural products have been optimized in evolution for interaction with biological systems and receptors. However, they have not necessarily been optimized for other desirable drug properties and thus can often be improved by structural modification. Using examples from the litera...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Floss HG

    更新日期:2006-06-25 00:00:00