Rewiring global regulator cAMP receptor protein (CRP) to improve E. coli tolerance towards low pH.

Abstract:

:Bioprocesses such as production of organic acids or acid hydrolysis of bioresources during biofuel production often suffer limitations due to microbial sensitivity under acidic conditions. Approaches for improving the acid tolerance of these microbes have mainly focused on using metabolic engineering tools. Here, we tried to improve strain acidic tolerance from its transcription level, i.e. we adopted error-prone PCR method to engineer global regulator cAMP receptor protein (CRP) of Escherichia coli to improve its performance at low pH. The best mutant AcM1 was identified from random mutagenesis libraries based on its growth performance. AcM1 almost doubled (0.113h(-1)) the growth rate of the control (0.062h(-1)) at pH 4.24. It also demonstrated better thermotolerance than the control at 48°C, whose growth was completely inhibited at this temperature. Quantitative real time reverse transcription PCR results revealed a stress response overlap among low pH stress-, oxidative stress- and osmotic stress-related genes. The chief enzyme responsible for cell acid tolerance, glutamate decarboxylase, demonstrated over twofold activity in AcM1 compared to the control. Differential binding properties of AcM1 mutant CRP with Class-I, II, and III CRP-dependent promoters suggested that modifications to native CRP may lead to transcription profile changes. Hence, we believe that transcriptional engineering of global regulator CRP can provide a new strain engineering alternative for E. coli.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Basak S,Geng H,Jiang R

doi

10.1016/j.jbiotec.2014.01.015

subject

Has Abstract

pub_date

2014-03-10 00:00:00

pages

68-75

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(14)00017-0

journal_volume

173

pub_type

杂志文章
  • Solvent-free enzymatic transesterification of ethyl ferulate and monostearin: optimized by response surface methodology.

    abstract::In this study, enzymatic transesterification of ethyl ferulate (EF) and monostearin for feruloylated lipids production was investigated. Enzyme screening and the effect of feruloyl acceptors on the transesterification were also studied. Effects of reaction variables (reaction temperatures, enzyme load, and reaction ti...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.01.013

    authors: Sun S,Song F,Bi Y,Yang G,Liu W

    更新日期:2012-12-15 00:00:00

  • Biotransformations of propenylbenzenes by an Arthrobacter sp. and its t-anethole blocked mutants.

    abstract::Propenylbenzenes are often used as starting materials in the chemical synthesis of aroma compounds and fine chemicals. In the present study, we demonstrate the ability of an Arthrobacter sp. to transform various structures of propenylbenzenes derived from essential oils to flavor, fragrance, and fine chemicals. Arthro...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(03)00141-x

    authors: Shimoni E,Baasov T,Ravid U,Shoham Y

    更新日期:2003-10-09 00:00:00

  • Cadmium toxicity in cowpea plant: Effect of foliar intervention of nano-TiO2 on tissue Cd bioaccumulation, stress enzymes and potential dietary health risk.

    abstract::The study was conducted to investigate the effects of foliar-intervention of nano-TiO2 on Cd toxicity in cowpea plants. Cowpea plants were exposed to Cd toxicity at 10 mg/kg soil for 21 days and afterwards, subjected to six episodes of foliar application of nano-TiO2 intervention. Results showed that foliar-applied na...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2020.01.009

    authors: Ogunkunle CO,Odulaja DA,Akande FO,Varun M,Vishwakarma V,Fatoba PO

    更新日期:2020-02-20 00:00:00

  • Solvent resistance pumps of Pseudomonas putida S12: Applications in 1-naphthol production and biocatalyst engineering.

    abstract::The solvent resistance capacity of Pseudomonas putida S12 was applied by using the organism as a host for biocatalysis and through cloning and expressing solvent resistant pump genes into Escherichia coli. P. putida S12 expressing toluene ortho mononooxygenase (TOM-Green) was used for 1-naphthol production in a water-...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.06.419

    authors: Janardhan Garikipati SV,Peeples TL

    更新日期:2015-09-20 00:00:00

  • Identification of a novel endogenous regulatory element in Chinese hamster ovary cells by promoter trap.

    abstract::The objective of this study was to identify and isolate endogenous promoters in Chinese hamster ovary (CHO) cells using a promoter trap approach. A promoter-less vector harboring a green fluorescent protein (GFP)-hygromycin resistance gene cassette was designed and transfected into CHO cells. Putative promoters were i...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.07.001

    authors: Chen J,Haverty J,Deng L,Li G,Qiu P,Liu Z,Shi S

    更新日期:2013-09-10 00:00:00

  • Evaluation of various serum and animal protein free media for the production of a veterinary rabies vaccine in BHK-21 cells.

    abstract::We have carried out the adaptation of BHK-21 cells to two serum free (Ex Cell 520 and HyQ PF CHO) and three animal protein free media: Ex Cell 302, HyQ PF CHO MPS and Rencyte BHK. After a direct switch or a gradual adaptation, we have achieved BHK-21 cells growth in the following media: HyQ PF CHO, HyQ PF CHO MPS, Ren...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(02)00009-3

    authors: Kallel H,Jouini A,Majoul S,Rourou S

    更新日期:2002-05-23 00:00:00

  • Process stability and microbial community structure in anaerobic hydrogen-producing microflora from food waste containing kimchi.

    abstract::Hydrogen production by the dark fermentation of food wastes is an economic and environmentally friendly technology to produce the clean energy source as well as to treat the problematic wastes. However, the long-term operations of the continuous anaerobic reactor for fermentative hydrogen production were frequently un...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2007.07.492

    authors: Jo JH,Jeon CO,Lee DS,Park JM

    更新日期:2007-09-15 00:00:00

  • Complete genome sequence of Bacillus thuringiensis YBT-1518, a typical strain with high toxicity to nematodes.

    abstract::Bacillus thuringiensis is a ubiquitous spore-forming bacterium and has been widely used as a biopesticide for controlling agricultural insects by the production of insecticidal crystal proteins (ICPs). B. thuringiensis YBT-1518 displays effective toxicity to nematodes. This strain harbors three nematicidal crystal pro...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.11.023

    authors: Wang P,Zhang C,Guo M,Guo S,Zhu Y,Zheng J,Zhu L,Ruan L,Peng D,Sun M

    更新日期:2014-02-10 00:00:00

  • Proteomic analysis of micro-scale bioreactors as scale-down model for a mAb producing CHO industrial fed-batch platform.

    abstract::The pharmaceutical production of recombinant proteins, such as monoclonal antibodies, is rather complex and requires proper development work. Accordingly, it is essential to develop appropriate scale-down models, which can mimic the corresponding production scale. In this work, we investigated the impact of the biorea...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2018.04.015

    authors: Bertrand V,Vogg S,Villiger TK,Stettler M,Broly H,Soos M,Morbidelli M

    更新日期:2018-08-10 00:00:00

  • Monitoring Azospirillum-wheat interactions using the gfp and gusA genes constitutively expressed from a new broad-host range vector.

    abstract::To monitor the colonization of wheat roots by Azospirillum brasilense, we constructed several plasmids based on the pBBR1 replicon expressing the gfp and gusA genes constitutively. Both genes were placed under control of the gentamycin resistance gene promoter resulting in high levels of expression in Escherichia coli...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(02)00108-6

    authors: Ramos HJ,Roncato-Maccari LD,Souza EM,Soares-Ramos JR,Hungria M,Pedrosa FO

    更新日期:2002-08-28 00:00:00

  • Application of polyhydroxyalkanoate (PHA) synthesis regulatory protein PhaR as a bio-surfactant and bactericidal agent.

    abstract::Polyhydroxyalkanoates (PHA), a family of diverse bio-polyesters, are produced by many bacteria as an energy and carbon storage material. PHA synthesis regulatory protein PhaR was reported to attach on the surface of intracellular PHA granules for convenience of synthesis regulation. PhaR was found to have an amphiphil...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.04.017

    authors: Ma HK,Liu MM,Li SY,Wu Q,Chen JC,Chen GQ

    更新日期:2013-06-20 00:00:00

  • Transcriptomic changes during tuber dormancy release process revealed by RNA sequencing in potato.

    abstract::Potato tuber dormancy release is a critical development process that allows potato to produce new plant. The first Illumina RNA sequencing to generate the expressed mRNAs at dormancy tuber (DT), dormancy release tuber (DRT) and sprouting tuber (ST) was performed. We identified 26,639 genes including 5,912 (3,450 up-re...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.01.019

    authors: Liu B,Zhang N,Wen Y,Jin X,Yang J,Si H,Wang D

    更新日期:2015-03-20 00:00:00

  • Engineering biotin prototrophic Corynebacterium glutamicum strains for amino acid, diamine and carotenoid production.

    abstract::The Gram-positive Corynebacterium glutamicum is auxotrophic for biotin. Besides the biotin uptake system BioYMN and the transcriptional regulator BioQ, this bacterium possesses functional enzymes for the last three reactions of biotin synthesis starting from pimeloyl-CoA. Heterologous expression of bioF from the Gram-...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.01.023

    authors: Peters-Wendisch P,Götker S,Heider SA,Komati Reddy G,Nguyen AQ,Stansen KC,Wendisch VF

    更新日期:2014-12-20 00:00:00

  • A combination of metabolome and transcriptome analyses reveals new targets of the Corynebacterium glutamicum nitrogen regulator AmtR.

    abstract::The effects of a deletion of the amtR gene, encoding the master regulator of nitrogen control in Corynebacterium glutamicum, were investigated by metabolome and transcriptome analyses. Compared to the wild type, different metabolite patterns were observed in respect to glycolysis, pentose phosphate pathway, citric aci...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2008.10.009

    authors: Buchinger S,Strösser J,Rehm N,Hänssler E,Hans S,Bathe B,Schomburg D,Krämer R,Burkovski A

    更新日期:2009-03-10 00:00:00

  • Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination.

    abstract::Generation and characterization of knockout clones is a widely used approach to evaluate the specific function of a gene product in Dictyostelium discoideum. The mutant clones are generally obtained by double homologous recombination in the target gene. A frequent limitation to obtaining mutants is the low frequency o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.08.023

    authors: Charette SJ,Cornillon S,Cosson P

    更新日期:2006-03-09 00:00:00

  • Transformation of terpenes using a Picea abies suspension culture.

    abstract::When subjected to a Picea abies suspension cell culture, beta-pinene, either one of the pure enantiomers or the racemate, was transformed mainly to trans-pinocarveol along with the minor products myrtenol, alpha-terpineol, pinocarvone, myrtenal and cis-pinocarveol. The absolute configuration of the major products corr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2003.10.009

    authors: Lindmark-Henriksson M,Isaksson D,Vanek T,Valterová I,Högberg HE,Sjödin K

    更新日期:2004-01-22 00:00:00

  • Secretion of correctly processed and folded pancreatic secretory trypsin inhibitor by Bacillus subtilis.

    abstract::We constructed a plasmid, designated pNPP126, containing a DNA sequence encoding a fusion protein composed of Bacillus amyloliquefaciens neutral protease prepeptide (signal peptide) and human pancreatic secretory trypsin inhibitor (hPSTI), where the mature hPSTI is accurately fused to the 3'-terminal of the prepeptide...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(92)90094-p

    authors: Nakayama A,Kobayashi H,Ando K,Hori M,Ohnishi T,Honjo M

    更新日期:1992-04-01 00:00:00

  • Cellulosomic profiling produced by Clostridium cellulovorans during growth on different carbon sources explored by the cohesin marker.

    abstract::Clostridium cellulovorans produces large extracellular enzyme complex, called cellulosomes. The diversity of the cellulosomal enzymes, which are secreted by C. cellulovorans that has been cultured on different carbon sources, such as Avicel, xylan, AXP (Avicel-xylan-pectin, 3:1:1) and cellobiose, was explored by two-d...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2009.11.020

    authors: Cho W,Jeon SD,Shim HJ,Doi RH,Han SO

    更新日期:2010-02-01 00:00:00

  • Direct oligonucleotide synthesis onto super-paramagnetic beads.

    abstract::Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedure of direct oligon...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.08.006

    authors: Jensen MA,Akhras MS,Fukushima M,Pourmand N,Davis RW

    更新日期:2013-09-20 00:00:00

  • Enrichment of Plasticicumulans acidivorans at pilot-scale for PHA production on industrial wastewater.

    abstract::A PHA producing microbial culture dominated by Plasticicumulans acidivorans was enriched in a pilot plant using fermented wastewater from the Mars candy bar factory. The pilot plant comprised (1) anaerobic fermentation, (2) enrichment of a PHA-producing microbial community and (3) accumulation for maximization of the ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.10.022

    authors: Tamisa J,Lužkov K,Jiang Y,van Loosdrecht MC,Kleerebezem R

    更新日期:2014-12-20 00:00:00

  • Development of an ELP-Z based mAb affinity precipitation process using scaled-down filtration techniques.

    abstract::In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based monoclonal antibody (mAb) affinity precipitation process is developed using scaled-down filtration techniques. Tangential flow filtration (TFF) is examined for the recovery of ELP-Z-mAb precipitates formed during the mAb binding st...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.09.020

    authors: Sheth RD,Bhut BV,Jin M,Li Z,Chen W,Cramer SM

    更新日期:2014-12-20 00:00:00

  • Biosynthesis of glycolate-based polyesters containing medium-chain-length 3-hydroxyalkanoates in recombinant Escherichia coli expressing engineered polyhydroxyalkanoate synthase.

    abstract::Glycolate(GL)-based polyesters were for the first time produced in the recombinant Escherichia coli fatty acid β-oxidation pathway reinforcing mutant LS5218, using extracellularly added GL as a monomer precursor. Cells expressing a Ser325Thr/Gln481Lys mutant of polyhydroxyalkanoate synthase (PhaC1STQK) from Pseudomona...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2011.07.040

    authors: Matsumoto K,Ishiyama A,Sakai K,Shiba T,Taguchi S

    更新日期:2011-12-10 00:00:00

  • Gene prediction and gene classes in Arabidopsis thaliana.

    abstract::Gene prediction methods for eukaryotic genomes still are not fully satisfying. One way to improve gene prediction accuracy, proven to be relevant for prokaryotes, is to consider more than one model of genes. Thus, we used our classification of Arabidopsis thaliana genes in two classes (CU(1) and CU(2)), previously del...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(00)00196-6

    authors: Mathé C,Déhais P,Pavy N,Rombauts S,Van Montagu M,Rouzé P

    更新日期:2000-03-31 00:00:00

  • The effect of the partition locus on plasmid stability and expression of a prolonged chemostat culture.

    abstract::The stability, copy number, and gene expression of the pBR322 plasmid containing the par-locus under prolonged cultivation were studied. In the initial stage of the experiment it was observed that the par-locus had a stabilization effect on plasmid maintenance. This observation was consistent with previously reported ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(91)90242-n

    authors: Weber AE,Yu P,San KY

    更新日期:1991-04-01 00:00:00

  • Production of the sesquiterpene (+)-valencene by metabolically engineered Corynebacterium glutamicum.

    abstract::The sesquiterpene (+)-valencene is an aroma compound of citrus fruits and is used to flavor foods and drinks. Biosynthesis of (+)-valencene starts from farnesyl pyrophosphate, an intermediate of carotenoid biosynthesis. Corynebacterium glutamicum, the workhorse of the million-ton scale amino acid industry, is naturall...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.05.032

    authors: Frohwitter J,Heider SA,Peters-Wendisch P,Beekwilder J,Wendisch VF

    更新日期:2014-12-10 00:00:00

  • Functionalization with ApoE-derived peptides enhances the interaction with brain capillary endothelial cells of nanoliposomes binding amyloid-beta peptide.

    abstract::Nanoliposomes containing phosphatidic acid or cardiolipin are able to target in vitro with very high affinity amyloid-β (Aβ), a peptide whose overproduction and progressive aggregation in the brain play a central role in the pathogenesis of Alzheimer's disease. However, the presence of the blood-brain barrier (BBB) se...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2011.06.037

    authors: Re F,Cambianica I,Sesana S,Salvati E,Cagnotto A,Salmona M,Couraud PO,Moghimi SM,Masserini M,Sancini G

    更新日期:2011-12-20 00:00:00

  • Influence of the extent of disruption of Bakers' yeast on protein adsorption in expanded beds.

    abstract::Expanded bed adsorption chromatography is used to capture the protein product of interest from a crude biological suspension directly, thereby eliminating the need for the removal of the cell debris. While this technique may replace three or four unit operations in a typical downstream process for biological product r...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2007.07.724

    authors: Balasundaram B,Harrison ST

    更新日期:2008-02-01 00:00:00

  • Complete genome sequencing of Agrobacterium sp. H13-3, the former Rhizobium lupini H13-3, reveals a tripartite genome consisting of a circular and a linear chromosome and an accessory plasmid but lacking a tumor-inducing Ti-plasmid.

    abstract::Agrobacterium sp. H13-3, formerly known as Rhizobium lupini H13-3, is a soil bacterium that was isolated from the rhizosphere of Lupinus luteus. The isolate has been established as a model system for studying novel features of flagellum structure, motility and chemotaxis within the family Rhizobiaceae. The complete ge...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2011.01.010

    authors: Wibberg D,Blom J,Jaenicke S,Kollin F,Rupp O,Scharf B,Schneiker-Bekel S,Sczcepanowski R,Goesmann A,Setubal JC,Schmitt R,Pühler A,Schlüter A

    更新日期:2011-08-20 00:00:00

  • Avian multiple inositol polyphosphate phosphatase is an active phytase that can be engineered to help ameliorate the planet's "phosphate crisis".

    abstract::Contemporary phytase research is primarily concerned with ameliorating the problem of inadequate digestion of inositol hexakisphosphate (phytate; InsP6) in monogastric farm animal feed, so as to reduce the pollution that results from the high phosphate content of the manure. In the current study we pursue a new, safe ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2006.04.028

    authors: Cho J,Choi K,Darden T,Reynolds PR,Petitte JN,Shears SB

    更新日期:2006-11-01 00:00:00

  • Heterologous production of different styrene oxide isomerases for the highly efficient synthesis of phenylacetaldehyde.

    abstract::The styrene oxide isomerase (SOI, StyC) represents a key enzyme of the styrene-degrading pathway and has been discussed as promising biocatalyst during recent studies. The enzyme enables the synthesis of pure phenylacetaldehyde from styrene oxide. In this study the native as well as the corresponding codon-optimized g...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.04.038

    authors: Oelschlägel M,Richter L,Stuhr A,Hofmann S,Schlömann M

    更新日期:2017-06-20 00:00:00