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Rapid and quantitative analysis of recombinant protein expression using pyrolysis mass spectrometry and artificial neural networks: application to mammalian cytochrome b5 in Escherichia coli.

Abstract：

:Recombinant Escherichia coli clones encoding between 0 and 6 copies of the mammalian cytochrome b5 gene were subjected to pyrolysis mass spectrometry (PyMS). To deconvolute the pyrolysis mass spectra so as to obtain quantitative information on the amount of cytochrome b5 produced fully-interconnected feedforward artificial neural networks (ANNs) were studied. It was found that the combination of PyMS and ANNs could be used to predict the amount of cytochrome b5 expressed in E. coli. PyMS is a novel, convenient and rapid method for the screening and analysis of microbial and other cultures producing recombinant proteins.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Goodacre R,Karim A,Kaderbhai MA,Kell DB

doi

10.1016/0168-1656(94)90088-4

subject

Has Abstract

pub_date

1994-05-15 00:00:00

pages

185-93

issue

2

eissn

0168-1656

issn

1873-4863

pii

0168-1656(94)90088-4

journal_volume

34

pub_type

杂志文章

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