Efficient extracellular production of κ-carrageenase in Escherichia coli: effects of wild-type signal sequence and process conditions on extracellular secretion.

abstract：:Penicillium purpurogenum secretes arabinofuranosidase to the growth medium. Highest levels of enzyme (1.0 U ml(-1)) are obtained when L-arabitol is used as carbon source, while 0.85 and 0.7 U ml(-1) are produced with sugar beet pulp and oat spelts xylan, respectively. By means of a zymogram, three bands with arabinofu...

journal_title：Journal of biotechnology

authors： De Ioannes P,Peirano A,Steiner J,Eyzaguirre J

更新日期：2000-01-21 00:00:00

abstract：:The growth of bacteria as structured aggregates termed biofilms leads to their protection from harsh environmental conditions such as physical and chemical stresses, shearing forces, and limited nutrient availability. Because of this highly adapted ability to survive adverse environmental conditions, bacterial biofilm...

journal_title：Journal of biotechnology

authors： Taylor PK,Yeung AT,Hancock RE

更新日期：2014-12-10 00:00:00

abstract：:In spite of high prices for fossil raw materials the production of biomass-based products is rarely economically successful today. Depending on the location feedstock prices are currently so high that products from renewable resources are not marketable when produced in existing process chains. Apart from the higher f...

journal_title：Journal of biotechnology

authors： Lyko H,Deerberg G,Weidner E

更新日期：2009-06-01 00:00:00

abstract：:The secreted pore-forming toxin listeriolysin O (LLO) is an essential virulence factor that allows the food-borne bacterial pathogen Listeria monocytogenes to escape from the phagocytic vacuole and reach the host cytosol. This protein belongs to the group of cholesterol-binding sulfhydryl-activated toxins, expressed b...

journal_title：Journal of biotechnology

authors： Giammarini C,Andreoni F,Amagliani G,Casiere A,Barocci S,Magnani M

更新日期：2004-04-08 00:00:00

abstract：:The addition of 5-40 mM L-glutamine to batch cultures of a murine hybridoma following the cessation of rapid growth significantly stimulated monoclonal antibody (mAb) synthesis and secretion per cell. Stimulation of mAb secretion following the cessation of rapid growth was also observed in response to addition of mito...

journal_title：Journal of biotechnology

authors： Flickinger MC,Goebel NK,Bibila T,Boyce-Jacino S

更新日期：1992-02-01 00:00:00

abstract：:In this paper, we report the chromium(VI) reduction by filtrates of Acidithiobacillus and Thiobacillus cultures. Chromium(VI) reduction by filtrates of A. ferrooxidans cultures under acidic conditions was higher than that observed for A. thiooxidans. However, at pH close to 7, chromium(VI) reduction by filtrates of T....

journal_title：Journal of biotechnology

authors： Allegretti P,Furlong J,Donati E

更新日期：2006-03-09 00:00:00

abstract：:Lentinula edodes, the popular shiitake mushroom, is one of the most important cultivated edible mushrooms. It is used as a food and for medicinal purposes. Here, we present the 46.1 Mb draft genome of L. edodes, comprising 13,028 predicted gene models. The genome assembly consists of 31 scaffolds. Gene annotation prov...

journal_title：Journal of biotechnology

authors： Shim D,Park SG,Kim K,Bae W,Lee GW,Ha BS,Ro HS,Kim M,Ryoo R,Rhee SK,Nou IS,Koo CD,Hong CP,Ryu H

更新日期：2016-04-10 00:00:00

abstract：:Stem borer resistant transgenic parental lines, involved in hybrid rice, were produced by Agrobacterium-mediated gene transfer method. Two pSB111 super-binary vectors containing modified cry1Ab/cry1Ac genes driven by maize ubiquitin promoter, and herbicide resistance gene bar driven by cauliflower mosaic virus 35S pro...

journal_title：Journal of biotechnology

authors： Ramesh S,Nagadhara D,Pasalu IC,Kumari AP,Sarma NP,Reddy VD,Rao KV

更新日期：2004-07-15 00:00:00

abstract：:Genetically engineered human osteosarcoma cells containing developmental endothelial locus-1 (del-1) gene were studied for production of Del-1, a protein that has the properties of an extracellular matrix protein and can regulate vascular morphogenesis and remodeling. Del-1 has been studied as a potential anti-angioge...

journal_title：Journal of biotechnology

authors： Chen C,Huang YL,Yang ST

更新日期：2002-07-17 00:00:00

abstract：:One hundred and seventy-eight new butanol-acetone producing bacteria related to saccharolytic clostridia were isolated from agricultural sources in Colombia and their fermentation potential was evaluated. Thirteen isolates produced more total solvents from glucose than Clostridium acetobutylicum ATCC 824. The isolates...

journal_title：Journal of biotechnology

authors： Montoya D,Spitia S,Silva E,Schwarz WH

更新日期：2000-04-28 00:00:00

abstract：:Chaperone co-expression and the fusion to different tags were used to modify the aggregation pattern of the putative serine protease CLIPB14 precipitated in Escherichia coli inclusion bodies. A set of common tags used in expression vectors has been selected, as well as two bacterial strains over-expressing the chapero...

journal_title：Journal of biotechnology

authors： Schrödel A,Volz J,de Marco A

更新日期：2005-10-17 00:00:00

abstract：:The aim of this study is to investigate the effects of an artificially controlled environment, particularly elevated total pressure, on net photosynthesis and respiration during plant growth. Pressure directly affects not only cells and organelles in leaves but also the diffusion coefficients and degrees of solubility...

journal_title：Journal of biotechnology

authors： Takeishi H,Hayashi J,Okazawa A,Harada K,Hirata K,Kobayashi A,Akamatsu F

更新日期：2013-10-20 00:00:00

abstract：:For pre-clinical evaluation of biotherapeutic candidates, protein production by transient gene expression (TGE) in Chinese Hamster Ovary (CHO) cells offers important advantages, including the capability of rapidly and cost-effectively generating recombinant proteins that are highly similar to those produced in stable ...

journal_title：Journal of biotechnology

authors： Stuible M,Burlacu A,Perret S,Brochu D,Paul-Roc B,Baardsnes J,Loignon M,Grazzini E,Durocher Y

更新日期：2018-09-10 00:00:00

abstract：:The importance of RNA-based regulation is becoming more and more evident. Genome-wide sequencing efforts have shown that the majority of the DNA in eukaryotic genomes is transcribed. Advanced high-throughput techniques like CLIP for the genome-wide detection of RNA-protein interactions have shown that post-transcripti...

journal_title：Journal of biotechnology

authors： Backofen R,Engelhardt J,Erxleben A,Fallmann J,Grüning B,Ohler U,Rajewsky N,Stadler PF

更新日期：2017-11-10 00:00:00

abstract：:A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was furthe...

journal_title：Journal of biotechnology

authors： Karmakar B,Vohra RM,Nandanwar H,Sharma P,Gupta KG,Sobti RC

更新日期：2000-07-14 00:00:00

abstract：:To explore the utility of glycosyltransferases as novel biocatalysts, we isolated the glycosyltransferase genes CaUGT2 and SbUGTA1 from Catharanthus roseus and Starmerella bombicola, respectively and heterologously expressed them in Escherichia coli. The purified recombinant proteins were assayed with a variety of sma...

journal_title：Journal of biotechnology

authors： Huang FC,Hinkelmann J,Schwab W

更新日期：2015-12-20 00:00:00

abstract：:A new and simple method was devised to obtain immunoglobulin VH genes directly from primary cultured B cells specific for a short peptide. Peptide-specific B cells were separated from splenocytes of peptide-immunized BALB/c mice with antigen-coated magnetic beads, and were cloned by a limitedly diluted culture in the ...

journal_title：Journal of biotechnology

authors： Takahashi Y,Ametani A,Totsuka M,Kaminogawa S

更新日期：1996-08-20 00:00:00

abstract：:Recently, a recombinant yeast pyruvate carboxylase expressed in the cytoplasm of BHK-21 cells was shown to reconstitute the missing link between glycolysis and TCA, thus increasing the flux of glucose into the TCA and resulting in a higher intracellular ATP content. Now, these metabolically engineered cells have been ...

journal_title：Journal of biotechnology

authors： Irani N,Beccaria AJ,Wagner R

更新日期：2002-02-28 00:00:00

abstract：:Two cyanobacterial strains, Synechocystis sp. PCC 6803 and Nostoc punctiforme ATCC 29133 were immobilized within magnesium phosphate based cements, showing a viability and activity for at least 4 weeks. These biohybrids are considered as an alternative photobioreactor material for bioremediation or an improved yield o...

journal_title：Journal of biotechnology

authors： Vorndran E,Lindberg P

更新日期：2016-04-10 00:00:00

abstract：:A fibrous-bed bioreactor containing the coculture of Pseudomonas putida and P. fluorescens immobilized in a fibrous matrix was developed to degrade benzene (B), toluene (T), ethylbenzene (E), and o-xylene (X) in synthetic waste streams. The kinetics of BTEX biodegradation by immobilized cells adapted in the fibrous-be...

journal_title：Journal of biotechnology

authors： Shim H,Yang ST

更新日期：1999-01-22 00:00:00

abstract：:Two affinity columns comprising immobilized concanavalin A (Con A), Con A-Sepharose and Con A-XP3507, were evaluated for their purifying ability for the glycoprotein allergen Ag7 from a partially purified extract of mugwort pollen. The most pronounced difference between the two columns was the nature of their nonspeci...

journal_title：Journal of biotechnology

authors： Nilsen BM,Smestad Paulsen B,Clonis Y,Mellbye KS

更新日期：1990-11-01 00:00:00

abstract：:Stem cell sensors have emerged as a promising technique to electrochemically monitor the functional status and viability of stem cells. However, efficient electrochemical analysis techniques are required for the development of effective electrochemical stem cell sensors. In the current study, we report a newly develop...

journal_title：Journal of biotechnology

authors： Yea CH,An JH,Kim J,Choi JW

更新日期：2013-06-20 00:00:00

abstract：:The polysaccharide xanthan which is produced by the γ-proteobacterium Xanthomonas campestris is used as a food thickening agent and rheologic modifier in numerous food, cosmetics and technical applications. Its great commercial importance stimulated biotechnological approaches to optimize the xanthan production. By ta...

journal_title：Journal of biotechnology

authors： Teckentrup J,Al-Hammood O,Steffens T,Bednarz H,Walhorn V,Niehaus K,Anselmetti D

更新日期：2017-09-10 00:00:00

abstract：:Using an RT-PCR approach a cDNA clone, designated Ms-Rac4 and putatively coding for a small GTPase was isolated from Medicago sativa. Ms-Rac4 and the earlier described Ms-Rac1 [Mol. Gen. Genet. 263 (2000) 761] belong to the class of GTP-binding Rho of plants (Rop) proteins. At the amino acid level they display all con...

journal_title：Journal of biotechnology

authors： Brecht M,Sewald K,Schiene K,Keen G,Fricke M,Sauer M,Niehaus K

更新日期：2004-08-26 00:00:00

abstract：:Cell culture optimization is a labor-intensive process requiring a large number of experiments to be conducted under varying conditions. Here we describe a high-throughput bioreactor system that allows 12 mini stirred-tank bioreactors to be operated simultaneously. All bioreactors are monitored by low-cost minimally i...

journal_title：Journal of biotechnology

authors： Ge X,Hanson M,Shen H,Kostov Y,Brorson KA,Frey DD,Moreira AR,Rao G

更新日期：2006-04-10 00:00:00

abstract：:To meet the food demand of growing population, agricultural productivity needs to be increased by employing safe strategies without harming ecosystem. Silver nanoparticle (AgNP) using a green approach has become a promising substitute to the synthetic pesticides to overcome pest menace. In this study, AgNPs were synth...

journal_title：Journal of biotechnology

authors： Vanti GL,Kurjogi M,Basavesha KN,Teradal NL,Masaphy S,Nargund VB

更新日期：2020-02-10 00:00:00

abstract：:Threonine aldolase (TA) catalyzes a reversible reaction, in which threonine is decomposed into glycine and acetaldehyde. The same enzyme can be used to catalyze aldol reaction between glycine and a variety of aromatic and aliphatic aldehydes, thus creating various alpha-amino-alcohols. Therefore, TA is a very promisin...

journal_title：Journal of biotechnology

authors： Wieteska L,Ionov M,Szemraj J,Feller C,Kolinski A,Gront D

更新日期：2015-04-10 00:00:00

abstract：:Environmental gradient is an important common issue during scale-up process for protein production. To address the dynamic regulatory mechanism of Aspergillus niger being exposed to inhomogeneous glucose concentrations, glucose perturbation were experimented on the steady state of A. niger chemostat culture, and dynam...

journal_title：Journal of biotechnology

authors： Li C,Shu W,Wang S,Liu P,Zhuang Y,Zhang S,Xia J

更新日期：2018-12-10 00:00:00

abstract：:The sesquiterpene (+)-valencene is an aroma compound of citrus fruits and is used to flavor foods and drinks. Biosynthesis of (+)-valencene starts from farnesyl pyrophosphate, an intermediate of carotenoid biosynthesis. Corynebacterium glutamicum, the workhorse of the million-ton scale amino acid industry, is naturall...

journal_title：Journal of biotechnology

authors： Frohwitter J,Heider SA,Peters-Wendisch P,Beekwilder J,Wendisch VF

更新日期：2014-12-10 00:00:00

abstract：:Flavonoids have notable biological activities and have been widely used in the medicinal and chemical industries. However, single-copy integration of heterologous pathway genes limits the production of flavonoids. In this work, we designed and constructed single-step integration of multiple flavonoid (2S)-naringenin b...

journal_title：Journal of biotechnology

authors： Li H,Gao S,Zhang S,Zeng W,Zhou J

更新日期：2021-01-10 00:00:00