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Vibrio cholerae periplasmic superoxide dismutase: isolation of the gene and overexpression of the protein.

Abstract：

:Superoxide dismutases are ubiquitous enzymes which play an important role in protecting cells against oxidative damage and which have also been shown to contribute to the pathogenicity of many bacterial species. Here we demonstrate that Vibrio cholerae, the causative agent of cholerae, expresses an active periplasmic Cu,Zn superoxide dismutase. Moreover, we have set up an expression system yielding large amounts of V. cholerae recombinant Cu,Zn superoxide dismutase in the periplasm of Escherichia coli and a procedure to obtain the enzyme in a highly purified form. Unlike the bovine enzyme, V. cholerae Cu,Zn superoxide dismutase has been proved to be highly resistant to inactivation by hydrogen peroxide. This property, which appears to be common to other bacterial enzymes of this class, might improve the ability of Cu,Zn superoxide dismutase to protect bacteria against the reactive oxygen species produced by phagocytes.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Gabbianelli R,Signoretti C,Marta I,Battistoni A,Nicolini L

doi

10.1016/j.jbiotec.2004.01.002

subject

Has Abstract

pub_date

2004-04-08 00:00:00

pages

123-30

issue

1-2

eissn

0168-1656

issn

1873-4863

pii

S0168165604000318

journal_volume

109

pub_type

杂志文章

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