PolI-driven integrative expression vectors for yeast.

abstract：:The present study aims to valorize chestnut burrs, an important lignocellulosic waste, through a biorefinery concept. A solid residue rich in glucan (41.36 ± 0.59 %) and lignin (39.06 ± 0.01 %) obtained from a previous process of pre-hydrolysis was subjected to four treatments with water or NaOH to enhance enzymatic h...

journal_title：Journal of biotechnology

authors： Costa-Trigo I,Paz A,Otero-Penedo P,Outeiriño D,Pérez Guerra N,Domínguez JM

更新日期：2021-01-18 00:00:00

abstract：:Pedobacter cryoconitis PAMC 27485, an aerobic, Gram-negative, facultatively psychrophilic bacterium, was isolated from Antarctic soil. Here we report the complete genome of P. cryoconitis PAMC 27485, which contains a type II CRISPR-Cas system and genes encoding useful enzymes (e.g. proteases). The genome sequence of P...

journal_title：Journal of biotechnology

authors： Lee J,Jung YJ,Lee HK,Hong SG,Kim OS

更新日期：2016-05-20 00:00:00

abstract：:Saccharum spontaneum L., a wild relative of sugarcane, is known for its adaptability to environmental stresses, particularly cold stress. In the present study, an attempt was made for transcriptome profiling of the low temperature (10°C) tolerant S. spontaneum clone IND 00-1037 collected from high altitude regions of ...

journal_title：Journal of biotechnology

authors： Dharshini S,Chakravarthi M,J AN,Manoj VM,Naveenarani M,Kumar R,Meena M,Ram B,Appunu C

更新日期：2016-08-10 00:00:00

abstract：:Phytopathogenic fungi penetrate plants by breaking down the cuticular barrier with cutinase. Cutinases are extracellular hydrolytic enzymes that degrade cutin, a polyester composed of hydroxy and epoxy fatty acids. Until now, cutinase has been recognized by its ability to release labeled cutin monomers or by a non-spe...

journal_title：Journal of biotechnology

authors： Degani O,Salman H,Gepstein S,Dosoretz CG

更新日期：2006-02-10 00:00:00

abstract：:Yarrowia lipolytica can accumulate large amounts of storage lipids and has considerable potential for the production of polyunsaturated fatty acids and other lipids for biofuels. When the nitrogen source is exhausted in the medium, the key intermediate, citrate, is converted to acetyl-CoA by ATP:citrate lyase (ACL) fo...

journal_title：Journal of biotechnology

authors： Zhang H,Zhang L,Chen H,Chen YQ,Chen W,Song Y,Ratledge C

更新日期：2014-12-20 00:00:00

abstract：:Glycodendrimers are relatively novel synthetic biomacromolecules that are made of biologically relevant carbohydrate ligands constructed at the periphery of a wide range of highly functionalized and repetitive scaffolds having varied molecular weights and structures. They were aimed to fill the gap between glycopolyme...

journal_title：Journal of biotechnology

authors： Roy R,Baek MG

更新日期：2002-05-01 00:00:00

abstract：:To improve enzymatic activity of Bacillus pumilus lipases, DNA shuffling was applied to two lipase genes from local B. pumilus isolates. Using a high-throughput activity assay, the mutant with highest activity was selected. This chimeric mutant (L3-3), carrying two crossover positions and three point mutations, has a ...

journal_title：Journal of biotechnology

authors： Akbulut N,Tuzlakoğlu Öztürk M,Pijning T,İşsever Öztürk S,Gümüşel F

更新日期：2013-03-10 00:00:00

abstract：:Turbulent flows in a single-stage and a two-stage impeller-stirred photobioreactor with a simple geometric configuration were analyzed using computational fluid dynamics. The trajectories of the microorganisms entrained in the flow field were traced by the particle tracking method. By projecting these trajectories ont...

journal_title：Journal of biotechnology

authors： Zhang T

更新日期：2013-10-10 00:00:00

abstract：:We cloned and characterized a preprochymosin cDNA from the abomasum of milk-fed kid goats. This cDNA contained an open reading frame that predicts a polypeptide of 381 amino acid residues, with a signal peptide and a proenzyme region of 16 and 42 amino acids, respectively. Comparison of the caprine preprochymosin sequ...

journal_title：Journal of biotechnology

authors： Vega-Hernández MC,Gómez-Coello A,Villar J,Claverie-Martín F

更新日期：2004-10-19 00:00:00

abstract：:Poly-l-cysteine (PLCys) is drawing attention as a potential sorbent of thiol (SH)-reactive toxic heavy metal ions in the wastewater and polluted soils. However, preparation of PLCys relies on chemically synthesized polymers, in which SH groups must be protected and deprotected prior to use. On the other hand, α-chymot...

journal_title：Journal of biotechnology

authors： Narai-Kanayama A,Hanaishi T,Aso K

更新日期：2012-02-10 00:00:00

abstract：:Dynamic miniaturized human multi-micro-organ bioreactor systems are envisaged as a possible solution for the embarrassing gap of predictive substance testing prior to human exposure. A rational approach was applied to simulate and design dynamic long-term cultures of the smallest possible functional human organ units,...

journal_title：Journal of biotechnology

authors： Sonntag F,Schilling N,Mader K,Gruchow M,Klotzbach U,Lindner G,Horland R,Wagner I,Lauster R,Howitz S,Hoffmann S,Marx U

更新日期：2010-07-01 00:00:00

abstract：:Particulate methane monooxygenase (pMMO) is a membrane protein embedded in the intracytoplasmic membrane of methane-oxidizing bacteria. Structural analysis of pMMO showed the existence of a hydrophilic region exposed outside of the bacterial membrane. To obtain information regarding the role of this hydrophilic region...

journal_title：Journal of biotechnology

authors： Miyaji A,Satou K,Baba T

更新日期：2020-11-10 00:00:00

abstract：:A DNA construct containing the human alpha 1-antitrypsin gene including 1.5 and 4 kb of 5' and 3' flanking sequences, was microinjected into the pronucleus of rabbit embryos. The recombinant human protein was (a) expressed in the blood circulation of F0 and F1 transgenic rabbits at an average concentration of 1 mg ml-...

journal_title：Journal of biotechnology

authors： Massoud M,Bischoff R,Dalemans W,Pointu H,Attal J,Schultz H,Clesse D,Stinnakre MG,Pavirani A,Houdebine LM

更新日期：1991-05-01 00:00:00

abstract：:In this work, we fabricated a system of integrated self-assembled layer of organosilane 3-mercaptopropyltrimethoxy silane (MPTS) on the screen printed electrode (SPE) and electrochemically deposited gold nanoparticle for Salmonella typhi detection employing Vi gene as a molecular marker. Thiolated DNA probe was immobi...

journal_title：Journal of biotechnology

authors： Das R,Sharma MK,Rao VK,Bhattacharya BK,Garg I,Venkatesh V,Upadhyay S

更新日期：2014-10-20 00:00:00

abstract：:Oxygen tension regulates the biological characteristics of hematopoietic stem and progenitor cells (HSPCs) by modulating intracellular reactive oxygen species (ROS). To better understand oxygen tension mechanism on HSPCs culture, gene expression analysis of human CD34(+)CD38(-) HSPCs was performed using microarrays. T...

journal_title：Journal of biotechnology

authors： Fan J,Cai H,Li Q,Du Z,Tan W

更新日期：2012-04-15 00:00:00

abstract：:This study demonstrates the applicability of pressurized stirred tank bioreactors for oxygen transfer enhancement in aerobic cultivation processes. The specific power input and the reactor pressure was employed as process variable. As model organism Escherichia coli, Arxula adeninivorans, Saccharomyces cerevisiae and ...

journal_title：Journal of biotechnology

authors： Knoll A,Bartsch S,Husemann B,Engel P,Schroer K,Ribeiro B,Stöckmann C,Seletzky J,Büchs J

更新日期：2007-10-31 00:00:00

abstract：:In this paper we report the development of a recombinant strain of the yeast Pichia pastoris, which secretes an anti-carcinoembryonic antigen single chain Fv (scFv) antibody fragment to the culture supernatant as a biologically active protein, at levels of 1.2 g l(-1). The yeast scFv was purified by IMAC, with a final...

journal_title：Journal of biotechnology

authors： Freyre FM,Vázquez JE,Ayala M,Canaán-Haden L,Bell H,Rodríguez I,González A,Cintado A,Gavilondo JV

更新日期：2000-01-21 00:00:00

abstract：:Gene prediction methods for eukaryotic genomes still are not fully satisfying. One way to improve gene prediction accuracy, proven to be relevant for prokaryotes, is to consider more than one model of genes. Thus, we used our classification of Arabidopsis thaliana genes in two classes (CU(1) and CU(2)), previously del...

journal_title：Journal of biotechnology

authors： Mathé C,Déhais P,Pavy N,Rombauts S,Van Montagu M,Rouzé P

更新日期：2000-03-31 00:00:00

abstract：:Reteplase is the third generation of thrombolytic medicine and has many advantages over commercial t-PA. However, over-expressing recombinant reteplase in E. coli always accumulates as inclusion bodies due to nine pairs of disulfide bonds formation that is the main obstacle for correct folding. In this paper, in order...

journal_title：Journal of biotechnology

authors： Zhuo XF,Zhang YY,Guan YX,Yao SJ

更新日期：2014-12-20 00:00:00

abstract：:The extraction of 13-hydroperoxide-lyase activity from mint leaves as well as its use for C6-aldehyde production was studied in this work. The enzyme cleaves 13(S)-hydroperoxy-C18 fatty acids into C6-aldehyde and C12-oxo-acid. Two mint species were tested: Mentha veridis and Mentha pulegium. The headspace injection me...

journal_title：Journal of biotechnology

authors： Gargouri M,Drouet P,Legoy MD

更新日期：2004-07-01 00:00:00

abstract：:Plasmid DNA purification strategies are often based on chromatographic processes, which often suffer from low dynamic binding capacities and poor productivity. The limitations of conventional chromatography media for adsorption of pDNA led us to the investigation of irregular polymeric microparticles with quaternary a...

journal_title：Journal of biotechnology

authors： Paril C,Horner D,Ganja R,Jungbauer A

更新日期：2009-04-20 00:00:00

abstract：:Thioalkalivibrio versutus D301 isolated from Soda Lake is a haloalkaliphilic and obligated chemolithoautotrophic Gram-negative bacterium. The strain has a good adaption to hyperhaline and highly alkaline environment and a powerful sulfur-oxidizing ability. Here, we present the complete genome sequence of T. versutus D...

journal_title：Journal of biotechnology

authors： Mu T,Zhou J,Yang M,Xing J

更新日期：2016-06-10 00:00:00

abstract：:Corynebacterium glutamicum produces succinate from glucose via the reductive tricarboxylic acid cycle under microaerobic and anaerobic conditions. We identified a NCgl2130 gene of C. glutamicum as a novel succinate exporter that functions in succinate production, and designated sucE1. sucE1 expression levels were high...

journal_title：Journal of biotechnology

authors： Fukui K,Koseki C,Yamamoto Y,Nakamura J,Sasahara A,Yuji R,Hashiguchi K,Usuda Y,Matsui K,Kojima H,Abe K

更新日期：2011-06-10 00:00:00

abstract：:A novel self-regulating bioreactor concept for sulfate reduction is proposed aiming for high biomass concentrations and treatment capacities. The system consists of a cell suspension of sulfate reducing bacteria in a continuous stirred tank reactor (30 degrees C) fed with a mixture of both electron donor and electron ...

journal_title：Journal of biotechnology

authors： Paulo PL,Kleerebezem R,Lettinga G,Lens PN

更新日期：2005-07-21 00:00:00

abstract：:Cell culture optimization is a labor-intensive process requiring a large number of experiments to be conducted under varying conditions. Here we describe a high-throughput bioreactor system that allows 12 mini stirred-tank bioreactors to be operated simultaneously. All bioreactors are monitored by low-cost minimally i...

journal_title：Journal of biotechnology

authors： Ge X,Hanson M,Shen H,Kostov Y,Brorson KA,Frey DD,Moreira AR,Rao G

更新日期：2006-04-10 00:00:00

abstract：:This study aimed to produce a cellulase blend and to evaluate its application in a simultaneous saccharification and fermentation (SSF) process for second generation ethanol production from sugar cane bagasse. The sugar cane bagasse was subjected to pretreatments (diluted acid and alkaline), as for disorganizing the l...

journal_title：Journal of biotechnology

authors： Maeda RN,Barcelos CA,Santa Anna LM,Pereira N Jr

更新日期：2013-01-10 00:00:00

abstract：:The PRNP gene encodes a cellular protein named prion, whose misfolded form has been implicated in a number of neuropathic diseases in mammals such as the Bovine Spongiform Encephalopathy (BSE) in cattle. BSE has brought devastating impact on the world economy and human health. Recently, several groups have performed t...

journal_title：Journal of biotechnology

authors： Wang S,Zhang K,Ding F,Zhao R,Li S,Li R,Xu L,Song C,Dai Y,Li N

更新日期：2013-02-20 00:00:00

abstract：:The transfer of genetic information between distantly or even unrelated organisms during evolution had been inferred from nucleotide sequence comparisons. These studies provided circumstantial evidence that in rare cases genes had been laterally transmitted amongst organisms of the domains bacteria, archaea and eukary...

journal_title：Journal of biotechnology

authors： Dröge M,Pühler A,Selbitschka W

更新日期：1998-09-17 00:00:00

abstract：:Rebuilding enzymes with high activities, stability and reusability has been constantly pursued for application of enzymaticcatalysis in wide field. Rebuilding enzymes by chemical approaches, including immobilization and chemicalmodification, represents a simple but effective route. Here, a class of highly stable and a...

journal_title：Journal of biotechnology

authors： Li L,Li H,Yan B,Yu S

更新日期：2019-02-10 00:00:00

abstract：:The growth, the alkaloid production, as well as the scopolamine/hyoscyamine ratio of two clones of belladonna hairy roots were studied. The effects of nitrate and ammonium concentrations on these cultures were investigated. A rise in ammonium concentration caused the decline of the hairy roots, while nitrate had a mar...

journal_title：Journal of biotechnology

authors： Bensaddek L,Gillet F,Saucedo JE,Fliniaux MA

更新日期：2001-01-23 00:00:00