Expression of active recombinant human alpha 1-antitrypsin in transgenic rabbits.

abstract：:AL (SpA A domain-PpL B3 domain), LD5 (PpL B3 domain-SpA D domain-PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L-D-L) and LD3 (PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L) are novel evolved Ig binding molecules (NEIBMs) derived from the in vitro molecular evolution of combinatorial phage libraries displaying rando...

journal_title：Journal of biotechnology

authors： He T,Ding YY,Feng JJ,Chen QL,Zhu HM,Peng H,Rui B,Li XY,Cao MM,Pan W

更新日期：2014-08-20 00:00:00

abstract：:Most expression systems used for the over production of many enzymes employ carbon catabolite repressible promoters and hence must use sugar free, rich complex media. Use of expression systems to overcome carbon repression opens an avenue for exploiting cheap carbon sources for the production of recombinant enzymes. A...

journal_title：Journal of biotechnology

authors： Nathan S,Nair M

更新日期：2013-12-01 00:00:00

abstract：:In the important industrial yeast Saccharomyces cerevisiae, galactose metabolism requires energy production by respiration; therefore, this yeast cannot metabolize galactose under strict anaerobic conditions. While the respiratory dependence of galactose metabolism provides benefits in terms of cell growth and populat...

journal_title：Journal of biotechnology

authors： Quarterman J,Skerker JM,Feng X,Liu IY,Zhao H,Arkin AP,Jin YS

更新日期：2016-07-10 00:00:00

abstract：:A solid-phase method is described to create subclones, suitable for DNA sequencing, from lambda or cosmid libraries. The purified target DNA is sonicated and two linkers, with one oligonucleotide biotinylated in the 5'-end, are ligated to the ends of the fragments produced by sonication. After size separation, the fra...

journal_title：Journal of biotechnology

authors： Hultman T,Uhlén M

更新日期：1994-06-30 00:00:00

abstract：:To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocy...

journal_title：Journal of biotechnology

authors： Zheng Y,Song X,Darby M,Liang Y,He L,Cai Z,Chen Q,Bi Y,Yang X,Xu J,Li Y,Sun Y,Lee RJ,Hou S

更新日期：2010-01-01 00:00:00

abstract：:Oxygen is a key substrate in animal cell metabolism. It has been reported that the oxygen uptake rate (OUR) is a good indicator of cellular activity, and even under some conditions, a good indicator of the number of viable cells. The measurement of OUR is difficult due to many different reasons. In particular, the ver...

journal_title：Journal of biotechnology

authors： Ruffieux PA,von Stockar U,Marison IW

更新日期：1998-08-12 00:00:00

abstract：:Ubiquitination is one of the most significant posttranslational modifications (PTMs). To evaluate the ability of an insect cell-free protein synthesis system to carry out ubiquitin (Ub) conjugation to in vitro translated proteins, poly-Ub chain formation was studied in an insect cell-free protein synthesis system. Pol...

journal_title：Journal of biotechnology

authors： Suzuki T,Ezure T,Ando E,Nishimura O,Utsumi T,Tsunasawa S

更新日期：2010-01-01 00:00:00

abstract：:Fast turnaround times are of utmost importance for biomedical reconnaissance, particularly regarding dangerous pathogens. Recent advances in sequencing technology and its devices allow sequencing within a short time frame outside stationary laboratories close to the epicenter of the outbreak. In our study, we evaluate...

journal_title：Journal of biotechnology

authors： Walter MC,Zwirglmaier K,Vette P,Holowachuk SA,Stoecker K,Genzel GH,Antwerpen MH

更新日期：2017-05-20 00:00:00

abstract：:We introduce an easy, fast and effective method to analyze the influence of genetically modified (GM) plants on soil and model organisms in the laboratory to substitute laborious and time consuming field trials. For the studies described here we focused on two GM plants of the so-called 3rd generation: GM plants produ...

journal_title：Journal of biotechnology

authors： Horn P,Schlichting A,Baum C,Hammesfahr U,Thiele-Bruhn S,Leinweber P,Broer I

更新日期：2017-09-10 00:00:00

abstract：:This study aimed at increasing the pyruvate productivity from a multi-vitamin auxotrophic yeast Torulopsis glabrata, by increasing the availability of NAD+. We examined two strategies for increasing availability of NAD+. To supplement nicotinic acid (NA), the precursor of NAD+; and to increase the activity of alcohol ...

journal_title：Journal of biotechnology

authors： Liu L,Li Y,Shi Z,Du G,Chen J

更新日期：2006-11-01 00:00:00

abstract：:Microbial host-pathogen interactions have been traditionally well studied at genetic and physiological levels, but cell-resolution analyses have been particularly scarce. This has been especially remarkable for intracellular parasites for two major reasons: first, the inherent loss of bacteria traceability once infect...

journal_title：Journal of biotechnology

authors： Medina C,Santero E,Gómez-Skarmeta JL,Royo JL

更新日期：2012-02-10 00:00:00

abstract：:We report the complete genome sequence of Amycolatopsis japonica MG417-CF17(T) (=DSM 44213(T)) which was identified as the producer of (S,S)-N,N'-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17(T) consists of two replicons: the chromosome (8,961,31...

journal_title：Journal of biotechnology

authors： Stegmann E,Albersmeier A,Spohn M,Gert H,Weber T,Wohlleben W,Kalinowski J,Rückert C

更新日期：2014-11-10 00:00:00

abstract：:The pyranose 2-oxidases from Trametes ochracea and Trametes pubescens share markedly similar amino acid sequences with identity of 93.4%. When expressed from the recombinant plasmids based on the same vector in the Escherichia coli host strain BL21(DE3) at higher growth temperatures, they differ strikingly in the form...

journal_title：Journal of biotechnology

authors： Maresová H,Palyzová A,Kyslík P

更新日期：2007-06-30 00:00:00

abstract：:Chimeric virus-like particles (VLP) are known as promising tools in the development of safe and effective subunit vaccines. Recently, a technology platform to produce VLP based on the small surface protein (dS) of the duck hepatitis B virus was established. In this study, chimeric VLP were investigated displaying the ...

journal_title：Journal of biotechnology

authors： Wetzel D,Barbian A,Jenzelewski V,Schembecker G,Merz J,Piontek M

更新日期：2019-12-20 00:00:00

abstract：:The filamentous ascomycete fungus Cryphonectria parasitica naturally secretes endothiapepsin, an aspartic proteinase. It is cultured on a commercial scale as a source of the milk-clotting enzyme for cheese making. Our objective was to increase enzyme production of an industrial C. parasitica strain by a new technique ...

journal_title：Journal of biotechnology

authors： Jara P,Delmas P,Razanamparany V,Olsen L,Dupin P,Bayol A,Bégueret J,Loison G

更新日期：1995-06-01 00:00:00

abstract：:Candida antarctica lipase B (CALB) has been employed as an efficient catalyst in the preparation of many flavor esters. A CALB-displaying yeast whole-cell biocatalyst could be an attractive alternative to commercial immobilized CALB because of its low-cost preparation and high enzymatic activity. We investigated the p...

journal_title：Journal of biotechnology

authors： Jin Z,Ntwali J,Han SY,Zheng SP,Lin Y

更新日期：2012-05-31 00:00:00

abstract：:A new and simple method was devised to obtain immunoglobulin VH genes directly from primary cultured B cells specific for a short peptide. Peptide-specific B cells were separated from splenocytes of peptide-immunized BALB/c mice with antigen-coated magnetic beads, and were cloned by a limitedly diluted culture in the ...

journal_title：Journal of biotechnology

authors： Takahashi Y,Ametani A,Totsuka M,Kaminogawa S

更新日期：1996-08-20 00:00:00

abstract：:One important question in microbiome analysis is how to assess the homogeneity of the microbial composition in a given environment, with respect to a given analysis method. Do different microbial samples taken from the same environment follow the same taxonomic distribution of organisms, or the same distribution of fu...

journal_title：Journal of biotechnology

authors： Huson DH,Steel M,El-Hadidi M,Mitra S,Peter S,Willmann M

更新日期：2017-05-20 00:00:00

abstract：:Group I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinan...

journal_title：Journal of biotechnology

authors： Minic R,Gavrovic-Jankulovic M,Petrusic V,Zivkovic I,Eijsink VG,Dimitrijevic L,Mathiesen G

更新日期：2015-04-10 00:00:00

abstract：:Efficient dynamic interactions among cofactor, enzymes and substrate molecules are of primary importance for multi-step enzymatic reactions with in situ cofactor regeneration. Here we showed for the first time that the above dynamic interactions could be significantly intensified by exerting an external alternating ma...

journal_title：Journal of biotechnology

authors： Zheng M,Su Z,Ji X,Ma G,Wang P,Zhang S

更新日期：2013-10-20 00:00:00

abstract：:A structured kinetic model that accounts for proteolytic degradation due to recombinant protein overexpression is introduced and its performance evaluated by comparison with previously reported fed-batch experimental data. This mathematical model contains an additional pool for a generic key precursor (in our case phe...

journal_title：Journal of biotechnology

authors： Ramírez DM,Bentley WE

更新日期：1999-05-28 00:00:00

abstract：:Detailed knowledge of mammalian cell culture proliferation kinetics is important to determine fed-batch strategies for industrial bioreactor operations. In particular, predicting the end of exponential proliferation in batch culture is a critical process parameter during culture scale-up. Using automated flow cytometr...

journal_title：Journal of biotechnology

authors： Sitton G,Srienc F

更新日期：2008-06-01 00:00:00

abstract：:The circadian clock schedules processes in microalgae cells at suitable times in the day/night cycle. To gain knowledge about these biological time schedules, Neochloris oleoabundans was grown under constant light conditions and nitrogen limitation. Under these constant conditions, the only variable was the circadian ...

journal_title：Journal of biotechnology

authors： de Winter L,Schepers LW,Cuaresma M,Barbosa MJ,Martens DE,Wijffels RH

更新日期：2014-10-10 00:00:00

abstract：:The growth inhibition of a murine hybridoma mediated by ammonium chloride was shown to vary with the pH of the culture medium. Values for the initial media concentration causing 50% growth inhibition (IC50) ranged from 4 mM to 7.6 mM as the pH was reduced from 7.8 to 6.8. A significant negative correlation was observe...

journal_title：Journal of biotechnology

authors： Doyle C,Butler M

更新日期：1990-07-01 00:00:00

abstract：:Myostatin (MSTN), a member of transforming growth factor-beta superfamily is a negative regulator of the skeletal muscle growth. It suppresses the proliferation and differentiation of myoblast cells. Dysfunction of MSTN gene either by natural mutation or induced through genetic manipulation (knockout or knockdown) has...

journal_title：Journal of biotechnology

authors： Jain H,Singh S,Kadam M,Sarkhel BC

更新日期：2010-01-15 00:00:00

abstract：:Two extra-cellular endoxylanases (Xyl Ia and Ib) were purified to homogeneity from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. Xyl Ia and Ib, having a molecular mass of approximately 53 kDa and pI of 5.2 and 4.8, respectively, were optimally active at 75 degrees C and at pH 6.0. They were st...

journal_title：Journal of biotechnology

authors： Chadha BS,Ajay BK,Mellon F,Bhat MK

更新日期：2004-04-29 00:00:00

abstract：:The solvent resistance capacity of Pseudomonas putida S12 was applied by using the organism as a host for biocatalysis and through cloning and expressing solvent resistant pump genes into Escherichia coli. P. putida S12 expressing toluene ortho mononooxygenase (TOM-Green) was used for 1-naphthol production in a water-...

journal_title：Journal of biotechnology

authors： Janardhan Garikipati SV,Peeples TL

更新日期：2015-09-20 00:00:00

abstract：:In this study, we developed a simple label-free method for the detection of catalase (CAT) using liquid crystals (LCs). The optical appearance of LCs changed from bright to dark when hydrogen peroxide was in contact with the dodecanal-doped nematic LC, 4-cyano-4'-pentylbiphenyl (5CB). Since hydrogen peroxide can oxidi...

journal_title：Journal of biotechnology

authors： Hu QZ,Jang CH

更新日期：2012-01-01 00:00:00

abstract：:Microarray analysis has become a popular and routine method in functional genomics. It is typical for such experiments to involve a small number of replicates, which causes unreliable estimates of the sample variance. Microarrays have fostered the development of new statistical methods to analyze data resulting from e...

journal_title：Journal of biotechnology

authors： Dondrup M,Hüser AT,Mertens D,Goesmann A

更新日期：2009-03-10 00:00:00

abstract：:A microorganism with the ability to form L-tryptophan from D,L-5-(3-indolyl-methyl)hydantoin (D,L-5-IMH) was isolated and identified as Arthrobacter sp. (DSM 3747). After isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high am...

journal_title：Journal of biotechnology

authors： Syldatk C,Mackowiak V,Höke H,Gross C,Dombach G,Wagner F

更新日期：1990-06-01 00:00:00