Abstract:
:In this work, we fabricated a system of integrated self-assembled layer of organosilane 3-mercaptopropyltrimethoxy silane (MPTS) on the screen printed electrode (SPE) and electrochemically deposited gold nanoparticle for Salmonella typhi detection employing Vi gene as a molecular marker. Thiolated DNA probe was immobilized on a gold nanoparticle (AuNP) modified SPE for DNA hybridization assay using methylene blue as redox (electroactive) hybridization indicator, and signal was monitored by differential pulse voltammetry (DPV) method. The modified SPE was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and atomic force microscopy (AFM) method. The DNA biosensor showed excellent performances with high sensitivity and good selectivity. The current response was linear with the target sequence concentrations ranging from 1.0 × 10(-11) to 0.5 × 10(-8)M and the detection limit was found to be 50 (± 2.1)pM. The DNA biosensor showed good discrimination ability to the one-base, two-base and three-base mismatched sequences. The fabricated genosensor could also be regenerated easily and reused for three to four times for further hybridization studies.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Das R,Sharma MK,Rao VK,Bhattacharya BK,Garg I,Venkatesh V,Upadhyay Sdoi
10.1016/j.jbiotec.2014.08.002subject
Has Abstractpub_date
2014-10-20 00:00:00pages
9-16eissn
0168-1656issn
1873-4863pii
S0168-1656(14)00799-8journal_volume
188pub_type
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