The effects of ROS-mediating oxygen tension on human CD34(+)CD38(-) cells induced into mature dendritic cells.


:Oxygen tension regulates the biological characteristics of hematopoietic stem and progenitor cells (HSPCs) by modulating intracellular reactive oxygen species (ROS). To better understand oxygen tension mechanism on HSPCs culture, gene expression analysis of human CD34(+)CD38(-) HSPCs was performed using microarrays. The CD34(+)CD38(-) HSPCs cultured under normoxia, hypoxia, or with N-acetyl cysteine (NAC, an ROS scavenger) were isolated for transcriptional profilings. Compared to normoxia group, 1 gene was up-regulated and 22 genes were down-regulated in hypoxia group, while 1 gene was up-regulated and 29 genes were down-regulated in NAC group. These differently expressed genes were involved in cell surface markers, blood activation and differentiation. The common down-regulated genes related to dendritic cells (DCs) maturation (CD80, CD86, and JAG1) were confirmed by real-time RT-PCR. Furthermore, the analysis of the phenotypes of DCs, including the DC-characteristic surface molecule CD1a, the costimulatory molecules CD80 and CD86, and HLA-DR, associated with the capacity of DCs to stimulate allogeneic T cells, showed that hypoxia-mediating ROS inhibited the potential of CD34(+)CD38(-) HSPCs differentiating to mature DCs. All these results demonstrated that hypoxia-reducing ROS down-regulated the genes driving CD34(+)CD38(-) HSPCs differentiation, which provides an interesting molecular hint to direct their development to DCs during cultures.


J Biotechnol


Journal of biotechnology


Fan J,Cai H,Li Q,Du Z,Tan W




Has Abstract


2012-04-15 00:00:00














  • The porcine gastrointestinal lamina propria: an appropriate target for mucosal immunisation?

    abstract::During the course of a lifetime, it has been calculated that we may consume between 100 and 700 tons of food. For the average British citizen, this is likely to include some 550 poultry, 36 pigs, 36 sheep, eight oxen, 10000 eggs and dairy products (milk, butter cheese, etc.) equivalent to 18 tonnes of milk. As if that...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Stokes CR,Bailey M

    更新日期:2000-09-29 00:00:00

  • Complete genome of biodegradable plastics-decomposing Roseateles depolymerans KCTC 42856(T) (=61A(T)).

    abstract::Roseateles depolymerans is a Gram-negative, rod-shaped, flagellated, obligately aerobic, photosynthetic bacterium that was isolated from the Hanamuro River, Ibaraki Prefecture, Japan. Here, we report the complete genome of R. depolymerans KCTC 42856(T) (=61A(T)=DSM 11813(T)=CCUG 48747(T)=NCIMB 13588(T)), which consist...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lee SH,Choe H,Kim SG,Park DS,Nasir A,Kim BK,Kim KM

    更新日期:2016-02-20 00:00:00

  • Isolation, taxonomic analysis, and phenotypic characterization of bacterial endophytes present in alfalfa (Medicago sativa) seeds.

    abstract::A growing body of evidence has reinforced the central role of microbiomes in the life of sound multicellular eukaryotes, thus more properly described as true holobionts. Though soil was considered a main source of plant microbiomes, seeds have been shown to be endophytically colonized by microorganisms thus representi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: López JL,Alvarez F,Príncipe A,Salas ME,Lozano MJ,Draghi WO,Jofré E,Lagares A

    更新日期:2018-02-10 00:00:00

  • Maximizing microbial degradation of perchlorate using a genetic algorithm: Media optimization.

    abstract::Microbial communities are under constant influence of physical and chemical components in ecosystems. Shifts in conditions such as pH, temperature or carbon source concentration can translate into shifts in overall ecosystem functioning. These conditions can be manipulated in a laboratory setup using evolutionary comp...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kucharzyk KH,Crawford RL,Paszczynski AJ,Soule T,Hess TF

    更新日期:2012-01-01 00:00:00

  • Measurement of intracellular (compartmental) pH by 31P NMR in Aspergillus niger.

    abstract::31P nuclear magnetic resonance (31P NMR) was used to monitor cytoplasmic and vacuolar pH values in the filamentous fungus Aspergillus niger. To obtain a homogeneous cell sample and to be able to perform long term in vivo NMR measurements A. niger mycelium was kept in a setup that allows perfusion of the cell plug with...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hesse SJ,Ruijter GJ,Dijkema C,Visser J

    更新日期:2000-01-28 00:00:00

  • Transcriptional profiles of Haloferax mediterranei based on nitrogen availability.

    abstract::The haloarchaeon Haloferax mediterranei is able to grow in the presence of different inorganic and organic nitrogen sources by means of the assimilatory pathway under aerobic conditions. In order to identify genes of potential importance in nitrogen metabolism and its regulation in the halophilic microorganism, we hav...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Esclapez J,Pire C,Camacho M,Bautista V,Martínez-Espinosa RM,Zafrilla B,Vegara A,Alcaraz LA,Bonete MJ

    更新日期:2015-01-10 00:00:00

  • Improved production of α-ketoglutaric acid (α-KG) by a Bacillus subtilis whole-cell biocatalyst via engineering of L-amino acid deaminase and deletion of the α-KG utilization pathway.

    abstract::We previously developed a novel one-step biotransformation process for the production of α-ketoglutarate (α-KG) from L-glutamic acid by a Bacillus subtilis whole-cell biocatalyst expressing an L-amino acid deaminase (pm1) of Proteus mirabilis. However, the biotransformation efficiency of this process was low owing to ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hossain GS,Li J,Shin HD,Liu L,Wang M,Du G,Chen J

    更新日期:2014-10-10 00:00:00

  • A non-pathogenic and optically high concentrated (R,R)-2,3-butanediol biosynthesizing Klebsiella strain.

    abstract::The objective of this work was to construct a non-pathogenic Klebsiella pneumonia strain that can produce optically high concentrated (R,R)-2,3-BDO. A K. pneumonia mutant lacking the pathogenic factor was used as the host strain. In order to construct a K. pneumonia strain that would biosynthesize high concentrated (R...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lee S,Kim B,Yang J,Jeong D,Park S,Lee J

    更新日期:2015-09-10 00:00:00

  • The BIORACK facility and its performance during the IML-2 Spacelab mission.

    abstract::The configuration and performance of the Biorack facility during the Second International Microgravity Laboratory mission (IML-2; 8-23 July 1995) is described in detail. During this Spacelab mission, Biorack flew with two incubators (22 degrees C and 37 degrees C), glovebox, cooler (5 degrees C) and four passive ther...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Manieri P,Brinckmann E,Brillouet C

    更新日期:1996-06-27 00:00:00

  • Complete genome sequence of Streptomyces sp. strain CFMR 7, a natural rubber degrading actinomycete isolated from Penang, Malaysia.

    abstract::Streptomyces sp. strain CFMR 7, which naturally degrades rubber, was isolated from a rubber plantation. Whole genome sequencing and assembly resulted in 2 contigs with total genome size of 8.248 Mb. Two latex clearing protein (lcp) genes which are responsible for rubber degrading activities were identified. ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Nanthini J,Chia KH,Thottathil GP,Taylor TD,Kondo S,Najimudin N,Baybayan P,Singh S,Sudesh K

    更新日期:2015-11-20 00:00:00

  • Investigative mining of sequence data for novel enzymes: a case study with nitrilases.

    abstract::Mining sequence data is increasingly important for biocatalysis research. However, when relying on sequence data alone, prediction of the reaction catalyzed by a specific protein sequence is often elusive, and substrate specificity is far from trivial. The present study demonstrated an approach of combining sequence d...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Seffernick JL,Samanta SK,Louie TM,Wackett LP,Subramanian M

    更新日期:2009-08-10 00:00:00

  • VEGF profiling and angiogenesis in human microtissues.

    abstract::Owing to its dual impact on tissue engineering (neovascularization of tissue implants) and cancer treatment (prevention of tumor-induced vascularization), management and elucidation of vascularization phenomena remain clinical priorities. Using a variety of primary human cells and (neoplastic) cell lines assembled in ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kelm JM,Diaz Sanchez-Bustamante C,Ehler E,Hoerstrup SP,Djonov V,Ittner L,Fussenegger M

    更新日期:2005-08-04 00:00:00

  • Induced prodrug activation by conditional protein degradation.

    abstract::Enzyme prodrug therapies hold potential as a targeted treatment option for cancer patients. However, off-target effects can be detrimental to patient health and represent a safety concern. This concern can be alleviated by including a failsafe mechanism that can abort the therapy in healthy cells. This feature can be ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Gaynor AS,Chen W

    更新日期:2017-10-20 00:00:00

  • Genetical identification of Akebia and Aristolochia species by using Pyrosequencing.

    abstract::This study was performed to determine if Akebia and Aristolochia species (Akebia quinata Decaisne and Aristolochia manshuriensis Kom.) could be identified by genetic analysis of Pyrosequencing method, which is used to assess genetic variation. The Pyrosequencing results of Akebia and Aristolochia species showed differ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Han S,Kim S,Yang CH,Seo J

    更新日期:2005-12-06 00:00:00

  • Biocatalytic production of (S)-2-aminobutanamide by a novel d-aminopeptidase from Brucella sp. with high activity and enantioselectivity.

    abstract::As the important chiral building block of levetiracetam, the synthesis of (S)-2-aminobutanamide has attracted a great deal of attention. The d-aminopeptidase catalyzed kinetic resolution of 2-aminobutanamide was demonstrated as an effective strategy for (S)-2-aminobutanamide production. In this study, a novel d-aminop...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Tang XL,Lu XF,Wu ZM,Zheng RC,Zheng YG

    更新日期:2018-01-20 00:00:00

  • Quantitative detection of Lactarius deliciosus extraradical soil mycelium by real-time PCR and its application in the study of fungal persistence and interspecific competition.

    abstract::Real-Time PCR has been applied to quantify extraradical soil mycelium of the edible ectomycorrhizal fungus Lactarius deliciosus in an interspecific competition experiment under greenhouse conditions. Couples of Pinus pinea seedlings inoculated with either L. deliciosus, Rhizopogon roseolus, or non-inoculated (control)...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Parladé J,Hortal S,Pera J,Galipienso L

    更新日期:2007-01-30 00:00:00

  • Diffusion in Pseudomonas aeruginosa biofilms: a pulsed field gradient NMR study.

    abstract::A Pseudomonas aeruginosa biofilm is studied with pulsed field gradient echo nuclear magnetic resonance. Although not all spectral components are assigned yet, the experimental results show that a biofilm consists of components with very different diffusion coefficients. The various biofilm components that give motiona...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Vogt M,Flemming HC,Veeman WS

    更新日期:2000-01-28 00:00:00

  • Optimizing high strength acetic acid bioprocess by cognitive methods in an unsteady state cultivation.

    abstract::Methods of adapting micro-organisms to an inhibiting factor in an active industrial bioprocess were examined with an acetic acid fermentation as model. With the aim of automatic control, a fuzzy-logic system was developed on the basis of the collected knowledge of skilled vinegar brewers. In a first step, this fuzzy s...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Arnold S,Becker T,Delgado A,Emde F,Enenkel A

    更新日期:2002-08-07 00:00:00

  • Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus.

    abstract::Pellet growth of Aspergillus terreus ATCC 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pel...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Casas López JL,Sánchez Pérez JA,Fernández Sevilla JM,Rodríguez Porcel EM,Chisti Y

    更新日期:2005-03-02 00:00:00

  • Elucidation of structure-function relationship of THCA and CBDA synthase from Cannabis sativaL.

    abstract::Cannabinoids are secondary natural products from the plant Cannabis sativaL. Therapeutic indications of cannabinoids currently comprise a significant area of medicinal research. We have expressed the Δ9-tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) recombinantly in Komagataella p...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zirpel B,Kayser O,Stehle F

    更新日期:2018-10-20 00:00:00

  • Differentiation of five tuna species by a multiplex primer-extension assay.

    abstract::A novel methodology based on analysis of mtDNA-cytb diagnostic sites was performed to discriminate four closely related species of Thunnus (Thunnus alalunga, Thunnus albacares, Thunnus obesus and Thunnus thynnus) and one species of Euthynnus (Katsuwonus pelamis) genus in raw and canned tuna. The primers used in the pr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Bottero MT,Dalmasso A,Cappelletti M,Secchi C,Civera T

    更新日期:2007-05-01 00:00:00

  • Rifampicin-resistance, rpoB polymorphism and RNA polymerase genetic engineering.

    abstract::Following its introduction in 1967, rifampicin has become a mainstay of therapy in the treatment of tuberculosis, leprosy and many other widespread diseases. Its potent antibacterial activity is due to specific inhibition of bacterial RNA polymerase. However, resistance to rifampicin was reported shortly after its int...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Alifano P,Palumbo C,Pasanisi D,Talà A

    更新日期:2015-05-20 00:00:00

  • Improved fermentative production of the compatible solute ectoine by Corynebacterium glutamicum from glucose and alternative carbon sources.

    abstract::The cyclic amino acid ectoine is a compatible solute serving as a protective substance against osmotic stress. Ectoine finds various applications due to its moisturizing effect. To avoid the disadvantages of the prevailing so-called "bacterial milking ectoine production process" caused by the high salt concentration, ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Pérez-García F,Ziert C,Risse JM,Wendisch VF

    更新日期:2017-09-20 00:00:00

  • Complete genome sequence of Streptomyces globisporus C-1027, the producer of an enediyne antibiotic lidamycin.

    abstract::Streptomyces globisporus C-1027 produces a nine-membered enediyne antitumor antibiotic lidamycin. Here we report the complete genome sequence of S. globisporus C-1027, which consists of a 7,608,611 bp linear chromosome, a 167,754 bp linear plasmid SGLP1 and a 7,234 bp circular plasmid pSGL1. The biosynthetic gene clus...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Li X,Lei X,Zhang C,Jiang Z,Shi Y,Wang S,Wang L,Hong B

    更新日期:2016-03-20 00:00:00

  • One-step eantioselective bioresolution for (S)-2-chlorophenylglycine methyl ester catalyzed by the immobilized Protease 6SD on multi-walled carbon nanotubes in a triphasic system.

    abstract::(S)-2-chlorophenylglycine methyl ester ((S)-1) is a key chiral building block of clopidogrel, which is a widely administered antiaggregatory and antithrombotic drug. Herein, Protease 6SD was covalently immobilized on multi-walled carbon nanotubes (MWCNT), and the as-prepared immobilizate P-6SD@NH2-MWCNT was applied in...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Weng CY,Wang DN,Ban SY,Zhai QY,Hu XY,Cheng F,Wang YJ,Zheng YG

    更新日期:2021-01-10 00:00:00

  • Co-expression of disulfide oxidoreductases DsbA/DsbC markedly enhanced soluble and functional expression of reteplase in Escherichia coli.

    abstract::Reteplase is the third generation of thrombolytic medicine and has many advantages over commercial t-PA. However, over-expressing recombinant reteplase in E. coli always accumulates as inclusion bodies due to nine pairs of disulfide bonds formation that is the main obstacle for correct folding. In this paper, in order...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zhuo XF,Zhang YY,Guan YX,Yao SJ

    更新日期:2014-12-20 00:00:00

  • Genome mining and biosynthesis of the Acyl-CoA:cholesterol acyltransferase inhibitor beauveriolide I and III in Cordyceps militaris.

    abstract::Ascomycete fungi Cordyceps are widely used in traditional Chinese medicine, and numerous investigations have been carried out to uncover their biological activities. However, primary researches on the physiological effects of Cordyceps were committed using crude extracts. At present, there are only a few compounds whi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Wang X,Gao YL,Zhang ML,Zhang HD,Huang JZ,Li L

    更新日期:2020-02-10 00:00:00

  • Secretion of correctly processed and folded pancreatic secretory trypsin inhibitor by Bacillus subtilis.

    abstract::We constructed a plasmid, designated pNPP126, containing a DNA sequence encoding a fusion protein composed of Bacillus amyloliquefaciens neutral protease prepeptide (signal peptide) and human pancreatic secretory trypsin inhibitor (hPSTI), where the mature hPSTI is accurately fused to the 3'-terminal of the prepeptide...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Nakayama A,Kobayashi H,Ando K,Hori M,Ohnishi T,Honjo M

    更新日期:1992-04-01 00:00:00

  • Impact of sample processing on human airways microbial metagenomes.

    abstract::Whole metagenome shotgun sequencing provides information about the gene content and the composition of microbial communities provided that the processing of the samples does not introduce a methodology-driven bias. We tested the impact of DNA isolation and storage period on the metagenome profile. Deep throat swabs we...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Wiehlmann L,Pienkowska K,Hedtfeld S,Dorda M,Tümmler B

    更新日期:2017-05-20 00:00:00

  • Three forms of thermostable lactose-hydrolase from Thermus sp. IB-21: cloning, expression, and enzyme characterization.

    abstract::Three thermostable lactose-hydrolases, namely, two beta-glycosidases (bglA and bglB) and one beta-galactosidase (bgaA) genes were cloned from the genomic library of Thermus sp. IB-21. The bglA, bglB, and bgaA consisted of 1311 bp (436 amino acid residues), 1296 bp (431 aa), and 1938 bp (645 aa) of nucleotides with pre...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kang SK,Cho KK,Ahn JK,Bok JD,Kang SH,Woo JH,Lee HG,You SK,Choi YJ

    更新日期:2005-04-06 00:00:00