Net P-removal deterioration in enriched PAO sludge subjected to permanent aerobic conditions.


:Recently, some research in the field of enhanced biological phosphorus removal (EBPR) has been focused on studying systems where the electron donor (substrate) and the electron acceptor (nitrate or oxygen) are present simultaneously. This can occur, for example, in a full scale wastewater treatment plant during heavy rainfall periods when the anaerobic hydraulic retention time is temporarily shortened. To study this situation that could induce EBPR failure, the operation of a sequencing batch reactor (SBR) working under alternating anaerobic-aerobic conditions with an enriched EBPR population (50% Candidatus Accumulibacter phosphatis and less than 1% Candidatus Competibacter phosphatis) was shifted to strict aerobic operation. Seven cycle studies were performed during the 11 days of aerobic operation. Net P-removal was observed in this aerobic SBR during the first 4 days of operation but the system could not achieve net-P removal after this period, although the microbial composition, in terms of percentage of Accumulibacter and Competibacter, did not change significantly. The observed changes in the different compounds analysed (phosphorus, acetate, glycogen and PHB) as well as in the OUR profile indicate that metabolic changes are produced for the adaptation of PAO to aerobic conditions.


J Biotechnol


Journal of biotechnology


Pijuan M,Guisasola A,Baeza JA,Carrera J,Casas C,Lafuente J




Has Abstract


2006-05-03 00:00:00














  • Monitoring Chinese hamster ovary cell culture by the analysis of glucose and lactate metabolism.

    abstract::Monitoring cell growth is crucial to the success of an animal cell culture process that can be accomplished by a variety of direct or indirect methodologies. Glucose is a major carbon and energy source for cultured mammalian cells in most cases, but glycolytic metabolism often results in the accumulation of lactate. G...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Tsao YS,Cardoso AG,Condon RG,Voloch M,Lio P,Lagos JC,Kearns BG,Liu Z

    更新日期:2005-08-22 00:00:00

  • Complete genome sequence of Bacillus methylotrophicus JJ-D34 isolated from deonjang, a Korean traditional fermented soybean paste.

    abstract::Bacillus methylotrophicus JJ-D34 showing good proteolytic and antipathogenic activities was isolated from doenjang, a Korean traditional fermented soybean paste. Here, we report the complete genome sequence of strain JJ-D34 harboring a 4,105,955 bp circular chromosome encoding 4044 genes with a 46.24% G+C content, whi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Jung JY,Chun BH,Moon JY,Yeo SH,Jeon CO

    更新日期:2016-02-10 00:00:00

  • The slow rotating centrifuge microscope NIZEMI--a versatile instrument for terrestrial hypergravity and space microgravity research in biology and materials science.

    abstract::NIZEMI (slow rotating centrifuge microscope) is a tool for optical investigations of small biological and non-biological specimens during variable accelerations. Two laboratory models for ground research designed for accelerations from 1 to 5 x g and 10 x g respectively are used for terrestrial research, especially i...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Friedrich UL,Joop O,Pütz C,Willich G

    更新日期:1996-06-27 00:00:00

  • A type 2 diacylglycerol acyltransferase accelerates the triacylglycerol biosynthesis in heterokont oleaginous microalga Nannochloropsis oceanica.

    abstract::Oleaginous microalgae have received a considerable attention as potential biofuel feedstock. However, lack of industry-suitable strain with lipid rich biomass limits its commercial applications. Targeted engineering of lipogenic pathways represents a promising strategy to enhance the efficacy of microalgal oil product...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Li DW,Cen SY,Liu YH,Balamurugan S,Zheng XY,Alimujiang A,Yang WD,Liu JS,Li HY

    更新日期:2016-07-10 00:00:00

  • Designing and construction of genetically encoded FRET-based nanosensor for qualitative analysis of digoxin.

    abstract::Nanobiotechnological improvements defined on the utilization of biological materials and principles have enormously partaken to revolutionize physical, chemical, and biological sciences. However, the exploration of plant nanobiotechnology is still in its outset. The search for novel tools to monitor plant biomolecules...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ambrin G,Kausar H,Ahmad A

    更新日期:2020-11-10 00:00:00

  • Implication of a mutation in the flavin binding site on the specific activity and substrate specificity of glycine oxidase from Bacillus subtilis produced by directed evolution.

    abstract::Directed evolution was used to expand the substrate specificity and functionality of glycine oxidase by using a high-throughput screening assay based on the 4-aminoantipyrine peroxidase system, with a coefficient of variance below 4%. After screening the library, one mutant with the desired changes was found. The muta...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Martínez-Martínez I,Navarro-Fernández J,García-Carmona F,Sánchez-Ferrer A

    更新日期:2008-01-01 00:00:00

  • Functional genomic analysis of the Bacillus subtilis Tat pathway for protein secretion.

    abstract::Protein secretion from Bacillus species is a major industrial production tool with a market of over $1 billion per year. However, standard export technologies, based on the well-characterised general secretory (Sec) pathway, are frequently inapplicable for the production of proteins. The recently discovered twin-argin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: van Dijl JM,Braun PG,Robinson C,Quax WJ,Antelmann H,Hecker M,Müller J,Tjalsma H,Bron S,Jongbloed JD

    更新日期:2002-09-25 00:00:00

  • Fine structure and enzymatic degradation of poly[(R)-3-hydroxybutyrate] and stereocomplexed poly(lactide) nanofibers.

    abstract::Fiber morphology and crystalline structure of poly[(R)-3-hydroxybutyrate] (P(3HB)) and stereocomplexed poly(lactide) (PLA) nanofibers were investigated by using scanning and transmission electron microscopies and X-ray and electron diffractions. In the P(3HB) nanofibers spun from less than 1 wt% 1,1,1,3,3,3-hexafluoro...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ishii D,Lee WK,Kasuya K,Iwata T

    更新日期:2007-11-01 00:00:00

  • Complete genome sequence of Lysinibacillus sphaericus LMG 22257, a strain with ureolytic activity inducing calcium carbonate precipitation.

    abstract::Microbiologically induced calcium carbonate precipitation shows the potential for use in bioremediation and construction consolidation, but the efficiency of this process must be improved. Lysinibacillus sphaericus LMG 22257 is a gram-positive ureolytic strain that has recently been applied for consolidating construct...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yan W,Xiao X,Zhang Y

    更新日期:2017-03-20 00:00:00

  • Enhancement of DNA vaccine potency by electroporation in vivo.

    abstract::The potential of electric current-mediated delivery technology to enhance DNA delivery and DNA vaccine potency was evaluated. Higher levels of reporter gene expression were observed in muscle cells of mice inoculated with luciferase or beta-galactosidase DNA followed by the application of electrical current, compared ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Selby M,Goldbeck C,Pertile T,Walsh R,Ulmer J

    更新日期:2000-09-29 00:00:00

  • Structures of almond hydroxynitrile lyase isoenzyme 5 provide a rationale for the lack of oxidoreductase activity in flavin dependent HNLs.

    abstract::Hydroxynitrile lyases (HNLs) catalyze the asymmetric addition of HCN to aldehydes producing enantiomerically pure cyanohydrins. These enzymes can be heterologously expressed in large quantities making them interesting candidates for industrial applications. The HNLs from Rosaceae evolved from flavin dependent dehydrog...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Pavkov-Keller T,Bakhuis J,Steinkellner G,Jolink F,Keijmel E,Birner-Gruenberger R,Gruber K

    更新日期:2016-10-10 00:00:00

  • Pphy--a cell-bound phytase from the yeast Pichia anomala: molecular cloning of the gene PPHY and characterization of the recombinant enzyme.

    abstract::The Pichia anomala gene PPHY, which codes for a cell-bound phytase, was isolated from genomic DNA by PCR, using oligonucleotide sequences derived from the N-terminal region of the purified phytase protein (Pphyp) and a degenerate primer derived from conserved sequences of yeast and fungal phytases as primers. The gene...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kaur P,Singh B,Böer E,Straube N,Piontek M,Satyanarayana T,Kunze G

    更新日期:2010-08-20 00:00:00

  • Production of 5-methyluridine by immobilized thermostable purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus JTS 859.

    abstract::5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column reactor at 60 degree...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hori N,Watanabe M,Sunagawa K,Uehara K,Mikami Y

    更新日期:1991-02-01 00:00:00

  • Genome improvement of the acarbose producer Actinoplanes sp. SE50/110 and annotation refinement based on RNA-seq analysis.

    abstract::Actinoplanes sp. SE50/110 is the natural producer of acarbose, which is used in the treatment of diabetes mellitus type II. However, until now the transcriptional organization and regulation of the acarbose biosynthesis are only understood rudimentarily. The genome sequence of Actinoplanes sp. SE50/110 was known befor...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Wolf T,Schneiker-Bekel S,Neshat A,Ortseifen V,Wibberg D,Zemke T,Pühler A,Kalinowski J

    更新日期:2017-06-10 00:00:00

  • Active fusions of Cas9 orthologs.

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    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Josipović G,Zoldoš V,Vojta A

    更新日期:2019-08-10 00:00:00

  • Optimizing high strength acetic acid bioprocess by cognitive methods in an unsteady state cultivation.

    abstract::Methods of adapting micro-organisms to an inhibiting factor in an active industrial bioprocess were examined with an acetic acid fermentation as model. With the aim of automatic control, a fuzzy-logic system was developed on the basis of the collected knowledge of skilled vinegar brewers. In a first step, this fuzzy s...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Arnold S,Becker T,Delgado A,Emde F,Enenkel A

    更新日期:2002-08-07 00:00:00

  • Microbial biomass production from rice straw hydrolysate in airlift bioreactors.

    abstract::Rice straw is a by-product of rice production, and a great bioresource as raw biomass material for manufacturing value-adding protein for animal feedstock, which has been paid more and more attention. In the present work, utilizing rice straw hydrolysate as a substrate for microbial biomass production in 11.5L externa...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zheng YG,Chen XL,Wang Z

    更新日期:2005-09-10 00:00:00

  • Solid-phase genetic engineering with DNA immobilized on a gold surface.

    abstract::A novel method for immobilizing large DNA fragments on a solid surface was developed. A mixed self-assembled monolayer of thiolated single-stranded DNA with inert alkanethiol was generated on a gold (Au) surface through the Au-S reaction. Surface-tethered DNA generated by this method was compatible with various geneti...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kim JH,Hong JA,Yoon M,Yoon MY,Jeong HS,Hwang HJ

    更新日期:2002-07-03 00:00:00

  • Expression of the porcine circovirus type 2 capsid protein subunits and application to an indirect ELISA.

    abstract::Porcine circovirus type 2 (PCV2) is considered to be associated with post-weaning multisystemic wasting syndrome (PMWS), which is a newly emerged economically important swine disease. The entire coding region of open reading frame 2 (ORF2), encoding the viral capsid protein (Cap), of PCV2 was cloned and sequenced from...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Wu PC,Chien MS,Tseng YY,Lin J,Lin WL,Yang CY,Huang C

    更新日期:2008-01-01 00:00:00

  • Complete genome sequence of the methanogenic neotype strain Methanobacterium formicicum MF(T.).

    abstract::The neotype strain Methanobacterium formicicum MF(T) (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074bp in size, featuring a GC content of 41.23%. M. fo...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Maus I,Stantscheff R,Wibberg D,Stolze Y,Winkler A,Pühler A,König H,Schlüter A

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  • Biostoning of denims by Penicillium occitanis (Pol6) cellulases.

    abstract::The Pol6 mutant of Penicillium occitanis, secreting a large quantity of cellulases, was cultivated in fermentor using a local paper pulp as an inducer substrate. A high titer of extracellular cellulase activity was reached after a fed batch process: 23 IU x ml(-1) filter paper activity, 21 IU x ml(-1) CMCases activity...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Belghith H,Ellouz-Chaabouni S,Gargouri A

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  • In situ detoxification and continuous cultivation of dilute-acid hydrolyzate to ethanol by encapsulated S. cerevisiae.

    abstract::Dilute-acid lignocellulosic hydrolyzate was successfully fermented to ethanol by encapsulated Saccharomyces cerevisiae at dilution rates up to 0.5h(-1). The hydrolyzate was so toxic that freely suspended yeast cells could ferment it continuously just up to dilution rate 0.1h(-1), where the cells lost 75% of their viab...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Talebnia F,Taherzadeh MJ

    更新日期:2006-09-18 00:00:00

  • Bacterial ghosts: non-living candidate vaccines.

    abstract::Expression of cloned PhiX174 gene E in bacteria results in lysis of bacteria. It is unique among phage lysis systems as it introduces a transmembrane tunnel structure through the cell envelope complex of Gram-negative bacteria. The resulting bacterial ghosts have intact envelope structures devoid of cytoplasmic conten...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Szostak MP,Hensel A,Eko FO,Klein R,Auer T,Mader H,Haslberger A,Bunka S,Wanner G,Lubitz W

    更新日期:1996-01-26 00:00:00

  • Effects of metabolic pathway gene copy numbers on the biosynthesis of (2S)-naringenin in Saccharomyces cerevisiae.

    abstract::Flavonoids have notable biological activities and have been widely used in the medicinal and chemical industries. However, single-copy integration of heterologous pathway genes limits the production of flavonoids. In this work, we designed and constructed single-step integration of multiple flavonoid (2S)-naringenin b...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Li H,Gao S,Zhang S,Zeng W,Zhou J

    更新日期:2021-01-10 00:00:00

  • ELP-z and ELP-zz capturing scaffolds for the purification of immunoglobulins by affinity precipitation.

    abstract::The increasing demand of monoclonal antibodies for therapeutic applications along with the high manufacturing cost have made it necessary to evaluate better process options and technologies for their purification. Affinity precipitation is an attractive alternative to traditional chromatographic methods by affording e...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Madan B,Chaudhary G,Cramer SM,Chen W

    更新日期:2013-01-10 00:00:00

  • New strategy for rapid isolation of stable cell lines from DNA-transformed insect cells using fluorescence activated cell-sorting.

    abstract::A stably transformed insect cell expression system is superior to a baculovirus expression system, since the expression system is sustained and there is no cell lysis, but the isolation of cell lines producing recombinant proteins is time-consuming and laborious. In this study, we developed a technique for the rapid i...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kato T,Yoshizuka K,Park EY

    更新日期:2010-05-17 00:00:00

  • Production processes of recombinant IL-1 beta from Bacillus subtilis: comparison between intracellular and exocellular expression.

    abstract::The human IL-1 beta coding sequence derived from a cDNA library was inserted into two different plasmid expression vectors, pSM214 and pSM308, which promote the synthesis of recombinant products intracellularly and exocellularly, respectively. The hybrid constructs pSM261 and pSM320 were obtained. Bacillus subtilis SM...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Bellini AV,Galli G,Fascetti E,Frascotti G,Branduzzi P,Lucchese G,Grandi G

    更新日期:1991-05-01 00:00:00

  • Antigen handling in the gastrointestinal lamina propria.

    abstract::Enteric infections associated with bacteria, viruses and parasites are a world health problem accounting for at least one billion episodes of diarrhoea and up to 10 million deaths in the Third World each year. The widely held conclusion that responses which are protective in the gastrointestinal tract are best stimula...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Stokes CR,Bailey M

    更新日期:1996-01-26 00:00:00

  • L-Lysine biosynthetic pathway of Methylophilus methylotrophus and construction of an L-lysine producer.

    abstract::Previously, we showed that the enzymes aspartokinase (AK) and dihydrodipicolinate synthase (DDPS), which are involved in L-lysine biosynthesis in the Gram-negative obligate methylotroph Methylophilus methylotrophus AS1, were inhibited by allosteric effectors, including L-lysine. To elucidate further the regulation of ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Tsujimoto N,Gunji Y,Ogawa-Miyata Y,Shimaoka M,Yasueda H

    更新日期:2006-07-13 00:00:00

  • Transcriptomic changes during tuber dormancy release process revealed by RNA sequencing in potato.

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    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Liu B,Zhang N,Wen Y,Jin X,Yang J,Si H,Wang D

    更新日期:2015-03-20 00:00:00