Transgenic rabbits as bioreactors for the production of human growth hormone.

Abstract:

:Gene farming is one of the most promising areas in modern biotechnology. To assay the potential usefulness of transgenic rabbits as bioreactors, one call embryos were microinjected with a chimeric gene comprising 5' sequences from mouse whey acidic protein gene (mWAP) linked to the human growth hormone (hGH) gene. Transgenic animals were obtained and the presence of the foreign protein was detected in the milk and serum of these animals at levels of up to 50 micrograms ml-1 and 0.6 ng ml-1, respectively. Founder transgenics were able to transmit the microinjected gene to the first filial generation in a Mendelian fashion. These results showed that transgenic rabbits could constitute a suitable system for the rapid production of recombinant proteins in the milk of lactating females.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Limonta JM,Castro FO,Martínez R,Puentes P,Ramos B,Aguilar A,Lleonart RL,de la Fuente J

doi

10.1016/0168-1656(95)00026-m

subject

Has Abstract

pub_date

1995-05-15 00:00:00

pages

49-58

issue

1

eissn

0168-1656

issn

1873-4863

pii

016816569500026M

journal_volume

40

pub_type

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