Abstract:
:Gene farming is one of the most promising areas in modern biotechnology. To assay the potential usefulness of transgenic rabbits as bioreactors, one call embryos were microinjected with a chimeric gene comprising 5' sequences from mouse whey acidic protein gene (mWAP) linked to the human growth hormone (hGH) gene. Transgenic animals were obtained and the presence of the foreign protein was detected in the milk and serum of these animals at levels of up to 50 micrograms ml-1 and 0.6 ng ml-1, respectively. Founder transgenics were able to transmit the microinjected gene to the first filial generation in a Mendelian fashion. These results showed that transgenic rabbits could constitute a suitable system for the rapid production of recombinant proteins in the milk of lactating females.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Limonta JM,Castro FO,Martínez R,Puentes P,Ramos B,Aguilar A,Lleonart RL,de la Fuente Jdoi
10.1016/0168-1656(95)00026-msubject
Has Abstractpub_date
1995-05-15 00:00:00pages
49-58issue
1eissn
0168-1656issn
1873-4863pii
016816569500026Mjournal_volume
40pub_type
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