Laser reflectance measurement for the online monitoring of Chlorella sorokiniana biomass concentration.

abstract：:MutS as a mismatch binding protein is a promising tool for SNP detection. Green fluorescent protein (GFP) is known as an excellent reporter domain. We constructed chimeric proteins consisting of MutS from Thermus thermophilus and GFPuv from Aequorea victoria by cloning the GFPuv gene into the plasmid vectors carrying ...

journal_title：Journal of biotechnology

authors： Stanisławska-Sachadyn A,Sachadyn P,Ihle K,Sydorczuk C,Wiejacha K,Kur J

更新日期：2006-01-24 00:00:00

abstract：:The Gram-positive Corynebacterium glutamicum is auxotrophic for biotin. Besides the biotin uptake system BioYMN and the transcriptional regulator BioQ, this bacterium possesses functional enzymes for the last three reactions of biotin synthesis starting from pimeloyl-CoA. Heterologous expression of bioF from the Gram-...

journal_title：Journal of biotechnology

authors： Peters-Wendisch P,Götker S,Heider SA,Komati Reddy G,Nguyen AQ,Stansen KC,Wendisch VF

更新日期：2014-12-20 00:00:00

abstract：:Soil salinity is one of the major environmental factors, influencing agricultural productivity of crops. As a non-edible and ideal oilseed crop, castor (Ricinus communis L.) has great industrial value in biofuel, but molecular mechanisms of salt stress regulation are still unknown. In this study, the differentially ex...

journal_title：Journal of biotechnology

authors： Lei P,Liu Z,Hu Y,Kim H,Liu S,Liu J,Xu L,Li J,Zhao Y,Yu Z,Qu Y,Huang F,Meng F

更新日期：2021-01-06 00:00:00

abstract：:Human interleukin-10 (hIL-10) has wide spectrum of anti-inflammatory activities and has shown a potential to be used for treatment of inflammatory or immune illness. In this study, transgenic mice that over-express human interleukin-10 (IL-10) in their milk were generated using a bovine beta-casein/human IL-10 hybrid ...

journal_title：Journal of biotechnology

authors： Sohn BH,Chang HG,Kang HS,Yoon H,Bae YS,Lee KK,Kim SJ

更新日期：2003-06-12 00:00:00

abstract：:Fast turnaround times are of utmost importance for biomedical reconnaissance, particularly regarding dangerous pathogens. Recent advances in sequencing technology and its devices allow sequencing within a short time frame outside stationary laboratories close to the epicenter of the outbreak. In our study, we evaluate...

journal_title：Journal of biotechnology

authors： Walter MC,Zwirglmaier K,Vette P,Holowachuk SA,Stoecker K,Genzel GH,Antwerpen MH

更新日期：2017-05-20 00:00:00

abstract：:A computer system solution for integration of a distributed bioreactor monitoring and control instrumentation on the laboratory scale is described. Bioreactors equipped with on-line analyzers for mass spectrometry, near-infrared spectroscopy, electrochemical probes and multi-array gas sensors and their respective soft...

journal_title：Journal of biotechnology

authors： Cimander C,Bachinger T,Mandenius CF

更新日期：2003-08-15 00:00:00

abstract：:An on-line model, estimating key state variables in bioprocesses, is utilized for control of fed-batch baker's yeast production. The state estimates are produced by balances and phenomenological expressions combined with on-line measurements. The goal of the control strategy is to maintain the highest possible glucose...

journal_title：Journal of biotechnology

authors： Ringbom K,Rothberg A,Saxén B

更新日期：1996-10-18 00:00:00

abstract：:Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedure of direct oligon...

journal_title：Journal of biotechnology

authors： Jensen MA,Akhras MS,Fukushima M,Pourmand N,Davis RW

更新日期：2013-09-20 00:00:00

abstract：:In earlier study from our group, cholera toxin B subunit had been expressed in tomato for developing a plant-based vaccine against cholera. In the present investigation, gene for accessory colonization factor (acf) subunit A, earlier reported to be essential for efficient colonization in the intestine, has been expres...

journal_title：Journal of biotechnology

authors： Sharma MK,Jani D,Thungapathra M,Gautam JK,Meena LS,Singh Y,Ghosh A,Tyagi AK,Sharma AK

更新日期：2008-05-20 00:00:00

abstract：:A solid-phase method is described to create subclones, suitable for DNA sequencing, from lambda or cosmid libraries. The purified target DNA is sonicated and two linkers, with one oligonucleotide biotinylated in the 5'-end, are ligated to the ends of the fragments produced by sonication. After size separation, the fra...

journal_title：Journal of biotechnology

authors： Hultman T,Uhlén M

更新日期：1994-06-30 00:00:00

abstract：:Each of the twelve enzymes for glycolytic fermentation, eleven from Escherichia coli and one from Saccharomyces cerevisiae, have been over-expressed in E. coli and purified with His-tags. Simple assays have been developed for each enzyme and they have been assembled for fermentation of glucose to ethanol. Phosphorus-3...

journal_title：Journal of biotechnology

authors： Stevenson BJ,Liu JW,Kuchel PW,Ollis DL

更新日期：2012-01-01 00:00:00

abstract：:The construction of expression vectors encoding either the human insulin A- or B-chains fused to a synthetic peptide and the temperature-induced expression of the recombinant genes in Escherichia coli are reported. Using this two-chain approach we also describe the separate isolation of the insulin A- and B-chains fro...

journal_title：Journal of biotechnology

authors： Schmidt M,Babu KR,Khanna N,Marten S,Rinas U

更新日期：1999-02-05 00:00:00

abstract：:Izumoring is a new concept for the bioproduction of all hexose sugars - 16 aldohexoses, eight ketohexoses and 10 hexitols -- using enzymatic and microbiological reactions. The biocatalysts necessary for the strategy were (i) d-tagatose 3-epimerase [which epimerizes all ketohexoses at C-3 of the sugar], (ii) polyol deh...

journal_title：Journal of biotechnology

authors： Izumori K

更新日期：2006-08-05 00:00:00

abstract：:Optically pure 1-phenyl-1,2-ethanediol is a very important chiral building block and intermediate in fine chemical and pharmaceutical industries. Reduction of 2-hydroxyacetophenone provides a straightforward approach to access these important compounds. In this study, two enantiocomplementary carbonyl reductases, BDHA...

journal_title：Journal of biotechnology

authors： Cui ZM,Zhang JD,Fan XJ,Zheng GW,Chang HH,Wei WL

更新日期：2017-02-10 00:00:00

abstract：:In this work, we fabricated a system of integrated self-assembled layer of organosilane 3-mercaptopropyltrimethoxy silane (MPTS) on the screen printed electrode (SPE) and electrochemically deposited gold nanoparticle for Salmonella typhi detection employing Vi gene as a molecular marker. Thiolated DNA probe was immobi...

journal_title：Journal of biotechnology

authors： Das R,Sharma MK,Rao VK,Bhattacharya BK,Garg I,Venkatesh V,Upadhyay S

更新日期：2014-10-20 00:00:00

abstract：:In vitro display technologies, such as mRNA display and DNA display are powerful tools to screen peptides and proteins with desired functions from combinatorial libraries in the fields of directed protein evolution and proteomics. When screening combinatorial libraries of polypeptides (phenotype), each of which is dis...

journal_title：Journal of biotechnology

authors： Doi N,Takashima H,Wada A,Oishi Y,Nagano T,Yanagawa H

更新日期：2007-09-15 00:00:00

abstract：:We report molecular dynamic simulations showing that a DNA molecule constituted by five unique bases can be spontaneously inserted into single walled carbon nanotube (SWCNT) in normal conditions (P, T and water environment) depending on the tube radius value. The van der Waals and electrostatic interactions play a cen...

journal_title：Journal of biotechnology

authors： Clavier A,Kraszewski S,Ramseyer C,Picaud F

更新日期：2013-03-10 00:00:00

abstract：:The cDNA coding for human proapolipoprotein A-I was expressed as a ubiquitin fusion under the control of the polyhedrin promoter in baculovirus-infected Sf9 Spodoptera frugiperda insect cells. The fusion protein was expressed at high level and was quantitatively cleaved in vivo. The cleaved product was purified and it...

journal_title：Journal of biotechnology

authors： Moguilevsky N,Guillaume JP,Varsalona F,Bulinckx M,Bollen A

更新日期：1994-01-15 00:00:00

abstract：:Structural composition and gene content of a biogas-producing microbial community from a production-scale biogas plant fed with renewable primary products was recently analyzed by means of a metagenome sequencing approach. To determine the transcriptionally active part of the same biogas community and to identify key ...

journal_title：Journal of biotechnology

authors： Zakrzewski M,Goesmann A,Jaenicke S,Jünemann S,Eikmeyer F,Szczepanowski R,Al-Soud WA,Sørensen S,Pühler A,Schlüter A

更新日期：2012-04-30 00:00:00

abstract：:An enzyme-responsive artificial chaperone system which employs an amphiphilic amylose primer (dodecyl maltopentaose, C12-MP) as a surfactant and phosphorylase b was designed to enable protein refolding. Effective refolding of carbonic anhydrase B after both heat denaturation (70 degrees C for 10min) and guanidine hydr...

journal_title：Journal of biotechnology

authors： Morimoto N,Ogino N,Narita T,Akiyoshi K

更新日期：2009-03-25 00:00:00

abstract：:In this paper, single-stranded (ss)DNA aptamers with capability to distinguish differentiated PC12 cells from normal PC12 cells were selected by subtractive systematic evolution of ligands by exponential enrichment (SELEX) method. Before each round of selection, randomized ssDNAs were incubated with regular PC12 cells...

journal_title：Journal of biotechnology

authors： Wang C,Zhang M,Yang G,Zhang D,Ding H,Wang H,Fan M,Shen B,Shao N

更新日期：2003-04-10 00:00:00

abstract：:Pedobacter cryoconitis PAMC 27485, an aerobic, Gram-negative, facultatively psychrophilic bacterium, was isolated from Antarctic soil. Here we report the complete genome of P. cryoconitis PAMC 27485, which contains a type II CRISPR-Cas system and genes encoding useful enzymes (e.g. proteases). The genome sequence of P...

journal_title：Journal of biotechnology

authors： Lee J,Jung YJ,Lee HK,Hong SG,Kim OS

更新日期：2016-05-20 00:00:00

abstract：:A gene encoding mature human insulin-like growth factor II (IGF-II) was constructed from the modified IGF-II cDNA sequence and two double-stranded synthetic oligodeoxynucleotide linkers. It was fused to a truncated lacZ gene such that IGF-II was expressed as part of C-terminus of beta-galactosidase. This fused lacZ'-I...

journal_title：Journal of biotechnology

authors： Rhee HJ,Lee YI,Yang KH

更新日期：1990-03-01 00:00:00

abstract：:Biodegradable scaffolds are of great value in tissue engineering. We have developed a method for fabricating patient-specific vascular scaffolds from a biocompatible and biodegradable polymer, poly(L-lactide-co-epsilon-caprolactone). This method's usefulness is due to flexibility in the choice of materials and vascula...

journal_title：Journal of biotechnology

authors： Uchida T,Ikeda S,Oura H,Tada M,Nakano T,Fukuda T,Matsuda T,Negoro M,Arai F

更新日期：2008-01-20 00:00:00

abstract：:The mTOR pathway is a conserved master regulator of translational activity that influences the fate of industrially relevant CHO cell cultures, yet its molecular mechanisms remain unclear. Interestingly, rapamycin specific inhibition of the mTOR pathway in CHO cells was found to down-regulate the small nucleolar RNA U...

journal_title：Journal of biotechnology

authors： Courtes FC,Gu C,Wong NS,Dedon PC,Yap MG,Lee DY

更新日期：2014-03-20 00:00:00

abstract：:4-vinylguaiacol, a kind of volatile phenolic compound with tobacco flavor, is widely used as a component of edible flavor and intermediate of medicine. Ferulic acid is usually used as substrate for the biosynthesis of 4-vinylguaiacol. However, the price of ferulic acid is high, leading to high production cost. In this...

journal_title：Journal of biotechnology

authors： Sun LH,Lv SW,Yu F,Li SN,He LY

更新日期：2018-09-10 00:00:00

abstract：:The extraction of 13-hydroperoxide-lyase activity from mint leaves as well as its use for C6-aldehyde production was studied in this work. The enzyme cleaves 13(S)-hydroperoxy-C18 fatty acids into C6-aldehyde and C12-oxo-acid. Two mint species were tested: Mentha veridis and Mentha pulegium. The headspace injection me...

journal_title：Journal of biotechnology

authors： Gargouri M,Drouet P,Legoy MD

更新日期：2004-07-01 00:00:00

abstract：:Three matrices were used for immobilizing the cytochrome c: Sepharose CL-4B, Silasorb SPH amine and a laboratory-prepared new matrix based on crosslinked triazine (2,4,6-tris(aminoethylamine)-1,3,5-triazine) (TAT). Cytochrome c was immobilized on the matrices by several procedures and the amount of incorporated cytoch...

journal_title：Journal of biotechnology

authors： Janiczek O,Pokorna B,Zemanova J,Mandl M

更新日期：2005-05-25 00:00:00

abstract：:An enzymatically-active fungal cellobiohydrolase I (CBH I) was first synthesized in a coupled reticulocyte lysate system lacking of glycosylation modification by the template DNA(Cbh1) in the presence of T7 RNA polymerase. The synthesized CBH I had the expected size (57 kDa) and catalyzed the substrate of p-nitropheny...

journal_title：Journal of biotechnology

authors： Wang J,Gao P

更新日期：2000-08-25 00:00:00

abstract：:A microcarrier culture system was established for a large-scale production of functional human endothelial cells. It has been difficult to cultivate human endothelial cells in large quantities for the reasons that specific growth factor and extracellular matrix are required for the survival and proliferation of the ce...

journal_title：Journal of biotechnology

authors： Tashiro S,Tsumoto K,Sano E

更新日期：2012-08-31 00:00:00