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Self-cloning in filamentous fungi: application to the construction of endothiapepsin overproducers in Cryphonectria parasitica.

Abstract：

:The filamentous ascomycete fungus Cryphonectria parasitica naturally secretes endothiapepsin, an aspartic proteinase. It is cultured on a commercial scale as a source of the milk-clotting enzyme for cheese making. Our objective was to increase enzyme production of an industrial C. parasitica strain by a new technique of self-cloning; it consisted in the screening for transformants producing higher levels of endothiapepsin and having integrated only the DNA fragment of interest. Such genetically improved strains that are devoid of any foreign genes should be more readily acceptable for the production of food-grade enzymes.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Jara P,Delmas P,Razanamparany V,Olsen L,Dupin P,Bayol A,Bégueret J,Loison G

doi

10.1016/0168-1656(95)00036-p

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

111-20

issue

2

eissn

0168-1656

issn

1873-4863

pii

0168-1656(95)00036-P

journal_volume

40

pub_type

杂志文章

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