Abstract：
:Survival of media-suspended porcine erythrocytes exposed to various hydrodynamic environments was investigated with and without such shear protectant additives as bovine serum albumin, dextran and the non-ionic surfactant Pluronic F68. Erythrocytes provided a model cell population with cells of a uniform size, metabolic state and shear tolerance. Because the cells were non-growing, any shear adaptation effects were avoided. Cell lysis was followed by microscopic counts or release of haemoglobin. The cells were susceptible to agitation damage in unaerated shake flasks agitated at 100 rpm or greater. Relative to additives-free operation, the presence of 0.1% (w/v) dextran or albumin prolonged cell survival, but Pluronic F68 actually enhanced cell lysis in flasks agitated at 100 rpm. The protective effect of the additives depended on the hydrodynamic conditions. The protective effect of albumin was demonstrated also in aerated conditions in a split-cylinder airlift bioreactor (aspect ratio of 8.8; riser-to-downcomer cross-sectional area ratio of 1.0; specific power input of 0.34 W m-3). Comparison of the cell lysis characteristics in the airlift device and the best case performance of the shake flask showed longer survival in the flask (100 rpm); however, the length of survival in the reactor (approx. 70 h) was sufficient for practical purposes. In all cases, the cell lysis pattern conformed initially to zero-order dependence in cell concentration, becoming first-order after varying degrees of exposure to hydrodynamic forces. Fatigue failure of cells was inferred.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Zhang Z,Chisti Y,Moo-Young Mdoi
10.1016/0168-1656(95)00111-8subject
Has Abstractpub_date
1995-11-21 00:00:00pages
33-40issue
1eissn
0168-1656issn
1873-4863pii
0168165695001118journal_volume
43pub_type
杂志文章abstract：:CRISPR-Cpf1 is a type V CRISPR system that has recently been exploited for genome engineering purposes. Compared to the well-known Streptococcus pyogenes CRISPR-Cas9 system, the effector protein Cpf1 recognizes T-rich protospacer-adjacent motif (PAM) instead of G-rich PAM (used by CRISPR-Cas9), which could offer a sub...
journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2018-10-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2013-02-20 00:00:00
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journal_title：Journal of biotechnology
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更新日期：1993-02-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2016-08-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2015.11.015
更新日期：2016-01-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/0168-1656(95)00026-m
更新日期：1995-05-15 00:00:00
abstract：:A system for streptavidin detection using biotin conjugated to nano-sized bacterial magnetic particles (BMPs) has been developed. BMPs, isolated from magnetic bacteria, were used as magnetic markers for magnetic force microscopy (MFM) imaging. The magnetic signal was obtained from a single particle using MFM without a...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2005.06.028
更新日期：2005-11-21 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2005.08.014
更新日期：2006-02-24 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.jbiotec.2017.09.001
更新日期：2017-10-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2003.11.007
更新日期：2004-03-04 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/s0168-1656(98)00071-6
更新日期：1998-07-30 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2018.06.346
更新日期：2018-10-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2014.12.002
更新日期：2015-02-10 00:00:00
abstract：:Improving in vivo half-life and in vitro stability of protein-based therapeutics is a current challenge for the biopharmaceutical industry. In particular, recombinant human interferon alpha-2b (rhIFN-α2b), which belongs to a group of cytokines extensively used for the treatment of viral diseases and cancers, shows a p...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2016.01.018
更新日期：2016-03-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2011.10.002
更新日期：2012-01-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0168-1656(02)00034-2
更新日期：2002-06-13 00:00:00
abstract：:A new β-glucosidase gene (bglSp) was cloned from the ginsenoside converting Sphingomonas sp. strain 2F2 isolated from the ginseng cultivating filed. The bglSp consisted of 1344 bp (447 amino acid residues) with a predicted molecular mass of 49,399 Da. A BLAST search using the bglSp sequence revealed significant homolo...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2011.07.024
更新日期：2011-11-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2005.05.023
更新日期：2005-10-17 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2015.09.024
更新日期：2015-11-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2007.01.004
更新日期：2007-05-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2008-06-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/0168-1656(95)00036-p
更新日期：1995-06-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2013.07.003
更新日期：2013-11-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2013.10.022
更新日期：2013-12-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/s0168-1656(01)00277-2
更新日期：2001-07-12 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2006.03.013
更新日期：2006-09-18 00:00:00
abstract：:Nanoliposomes containing phosphatidic acid or cardiolipin are able to target in vitro with very high affinity amyloid-β (Aβ), a peptide whose overproduction and progressive aggregation in the brain play a central role in the pathogenesis of Alzheimer's disease. However, the presence of the blood-brain barrier (BBB) se...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2011.06.037
更新日期：2011-12-20 00:00:00
abstract：:The transfer of genetic information between distantly or even unrelated organisms during evolution had been inferred from nucleotide sequence comparisons. These studies provided circumstantial evidence that in rare cases genes had been laterally transmitted amongst organisms of the domains bacteria, archaea and eukary...
journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
doi：10.1016/s0168-1656(98)00105-9
更新日期：1998-09-17 00:00:00
abstract：:As the understanding of variation is the key to a good process and product quality one should pay attention to dynamics on the single-cell level. The basic idea of this approach was to qualify and quantify variations on the single-cell level during bioreactor cultivations by monitoring the expression of an eGFP tagged...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2011.05.003
更新日期：2011-07-20 00:00:00
abstract：:Biomass-derived d-xylose represents an economically interesting substrate for the sustainable microbial production of value-added compounds. The industrially important platform organism Corynebacterium glutamicum has already been engineered to grow on this pentose as sole carbon and energy source. However, all current...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2014.09.026
更新日期：2014-12-20 00:00:00
