Do-it-yourself histidine-tagged bovine enterokinase: a handy member of the protein engineer's toolbox.

abstract：:The enzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature of β-carboline (βC) alkaloid biosynthesis. NscbB is a rare microbial PSase discovered from a cryptic β-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplan...

journal_title：Journal of biotechnology

authors： Chen Q,Zhang S,Xie Y

更新日期：2018-09-10 00:00:00

abstract：:Catalytic properties and conformational stability of aminoacylase (N-acylamino acid amidohydrolase, EC 3.5.1.14) were studied in water-N,N-dimethylformamide (DMF) and water-dioxane solvent mixtures. Beside the prompt inhibition the solvents caused further inactivation during incubations. In the presence of 5% DMF cont...

journal_title：Journal of biotechnology

authors： Boross L,Kosáry J,Stefanovits-Bányai E,Sisak C,Szajáni B

更新日期：1998-11-18 00:00:00

abstract：:New emerging technologies delivering benefits in terms of process robustness and economy are an inevitable prerequisite for monoclonal antibody purification processes intensification. Caprylic acid was proven as an effective precipitating agent enabling efficient precipitaton of product- and process-related impurities...

journal_title：Journal of biotechnology

authors： Trapp A,Faude A,Hörold N,Schubert S,Faust S,Grob T,Schmidt S

更新日期：2018-08-10 00:00:00

abstract：:Two-stage fermentation process was used for lipid production by Lipomyces starkeyi AS 2.1560 in glucose solution without auxiliary nutrients. In the first stage, cells were cultivated in a nutrient-rich medium for propagation. In the second stage, cells were resuspended in glucose solution to achieve high cellular lip...

journal_title：Journal of biotechnology

authors： Lin J,Shen H,Tan H,Zhao X,Wu S,Hu C,Zhao ZK

更新日期：2011-04-10 00:00:00

abstract：:Peptide synthesis was carried out in a variety of organic solvents with low contents of water. The enzyme was deposited on the support material, celite, from an aqueous buffer solution. After evaporation of the water the biocatalyst was suspended in the reaction mixtures. The chymotrypsin-catalyzed reaction between Z-...

journal_title：Journal of biotechnology

authors： Clapés P,Adlercreutz P,Mattiasson B

更新日期：1990-09-01 00:00:00

abstract：:Specific delivery of TNF-α antagonist to the inflamed site can increase its efficacy and reduce the side effects. In this study, we constructed a bispecific diabody (BsDb) that targets TNF-α and ED-B-containing fibronectin (B-FN), a fibronectin isoform specifically expressed in the pannus of the inflamed joint in rheu...

journal_title：Journal of biotechnology

authors： Liu M,Xie M,Jiang S,Liu G,Li L,Liu D,Yang X

更新日期：2014-09-30 00:00:00

abstract：:Approaches using metabolic engineering to produce D-1, 2, 4-butanetriol (BT) from renewable biomass in microbial systems have achieved initial success. However, due to the lack of incomplete understanding of the complex branch pathway, the efficient fermentation system for BT production was difficult to develop. Here ...

journal_title：Journal of biotechnology

authors： Gao Q,Wang X,Hu S,Xu N,Jiang M,Ma C,Yang J,Xu S,Chen K,Ouyang P

更新日期：2019-02-20 00:00:00

abstract：:The growth inhibition of a murine hybridoma mediated by ammonium chloride was shown to vary with the pH of the culture medium. Values for the initial media concentration causing 50% growth inhibition (IC50) ranged from 4 mM to 7.6 mM as the pH was reduced from 7.8 to 6.8. A significant negative correlation was observe...

journal_title：Journal of biotechnology

authors： Doyle C,Butler M

更新日期：1990-07-01 00:00:00

abstract：:The aim of this study is to investigate the effects of an artificially controlled environment, particularly elevated total pressure, on net photosynthesis and respiration during plant growth. Pressure directly affects not only cells and organelles in leaves but also the diffusion coefficients and degrees of solubility...

journal_title：Journal of biotechnology

authors： Takeishi H,Hayashi J,Okazawa A,Harada K,Hirata K,Kobayashi A,Akamatsu F

更新日期：2013-10-20 00:00:00

abstract：:In this study, we used the homologous recombination protein RecA to locate a specific sequence on DNA. Single-stranded (ss) DNA (80-mer, 5'-biotinylated), complementary to the sequence of interest, was labeled with quantum dots (Qdots(®)) via biotin-avidin binding. The DNA was then mixed with RecA to form a fluorescen...

journal_title：Journal of biotechnology

authors： Kimura Y,Goto Y,Oana H,Washizu M

更新日期：2012-12-15 00:00:00

abstract：:Understanding the causes of discrepancies in pH readings of a sample can allow more robust pH control strategies to be implemented. It was found that 59.4% of differences between two offline pH measurement technologies for an historical dataset lay outside an expected instrument error range of ±0.02pH. A new variable,...

journal_title：Journal of biotechnology

authors： Ritchie EK,Martin EB,Racher A,Jaques C

更新日期：2017-06-10 00:00:00

abstract：:The expression of heterologous proteins in microorganisms using genetic recombination is still the high point in the development and exploitation of modern biotechnology. People can produce bioactive proteins from relatively cheap culture medium instead of expensive extraction. Host cell systems for the expression of ...

journal_title：Journal of biotechnology

authors： Yin J,Li G,Ren X,Herrler G

更新日期：2007-01-10 00:00:00

abstract：:A murine B-lymphocyte hybridoma (CC9C10) was adapted for growth in a serum-free medium. Supplementation of the medium with cis-unsaturated fatty acids (10-50 microM) improved the cell yield in the order oleic/linoleic > linoleic > oleic. Initial supplementation with the fatty acids also caused a significant increase (...

journal_title：Journal of biotechnology

authors： Butler M,Huzel N

更新日期：1995-04-15 00:00:00

abstract：:Macroscopic modelling of bioprocesses requires the determination of a biological reaction scheme and a kinetic model. The a priori selection of an appropriate kinetic model structure is usually made difficult by the lack of detailed bioprocess knowledge and the profusion of apparently similar biological kinetic laws. ...

journal_title：Journal of biotechnology

authors： Grosfils A,Vande Wouwer A,Bogaerts P

更新日期：2007-06-30 00:00:00

abstract：:Microbiologically induced calcium carbonate precipitation (MICCP) is a naturally occurring biological process that has shown its potential in remediation of a wide range of structural damages including concrete cracks. In this study, genetically engineered microorganisms, capable of producing extracellular polymeric s...

journal_title：Journal of biotechnology

authors： Bergdale TE,Pinkelman RJ,Hughes SR,Zambelli B,Ciurli S,Bang SS

更新日期：2012-10-31 00:00:00

abstract：:Superoxide dismutases are ubiquitous enzymes which play an important role in protecting cells against oxidative damage and which have also been shown to contribute to the pathogenicity of many bacterial species. Here we demonstrate that Vibrio cholerae, the causative agent of cholerae, expresses an active periplasmic ...

journal_title：Journal of biotechnology

authors： Gabbianelli R,Signoretti C,Marta I,Battistoni A,Nicolini L

更新日期：2004-04-08 00:00:00

abstract：:Deracemization of D,L-phosphinothricin (D,L-PPT) is one of the most promising routes for preparation of optically pure L-PPT. In this work, an efficient multi-enzyme redox cascade was developed for deracemization ofPPT, which includes oxidative reaction and reductive reaction. The oxidative reaction catalyzing oxidati...

journal_title：Journal of biotechnology

authors： Cao CH,Gong H,Dong Y,Li JM,Cheng F,Xue YP,Zheng YG

更新日期：2021-01-10 00:00:00

abstract：:The potential of electric current-mediated delivery technology to enhance DNA delivery and DNA vaccine potency was evaluated. Higher levels of reporter gene expression were observed in muscle cells of mice inoculated with luciferase or beta-galactosidase DNA followed by the application of electrical current, compared ...

journal_title：Journal of biotechnology

authors： Selby M,Goldbeck C,Pertile T,Walsh R,Ulmer J

更新日期：2000-09-29 00:00:00

abstract：:The poly(beta-hydroxybutyrate) (PHB) biosynthetic genes of Ralstonia eutropha that are organized in a single operon (phaCAB) have been cloned in Escherichia coli, where the expression of the genes in the wild-type pha operon from plasmid pTZ18U-PHB leads to the formation of 50-80% PHB/celldry mass when the cells are g...

journal_title：Journal of biotechnology

authors： Resch S,Gruber K,Wanner G,Slater S,Dennis D,Lubitz W

更新日期：1998-10-27 00:00:00

abstract：:Following the discovery of RNA interference (RNAi) and related phenomena, novel regulatory processes, attributable to small non-protein-coding RNAs, continue to emerge. Capitalizing on the ability of artificial short interfering RNAs (siRNAs) to trigger degradation of specific target transcripts, and thereby silence d...

journal_title：Journal of biotechnology

authors： Malphettes L,Fussenegger M

更新日期：2006-08-05 00:00:00

abstract：:Vigna radiata (mung bean) is an important crop plant and is a major protein source in developing countries. Mung bean 8S globulins constitute nearly 90% of total seed storage protein and consist of three subunits designated as 8SGα, 8SGα' and 8SGβ. The 5'-flanking sequences of 8SGα' has been reported to confer high ex...

journal_title：Journal of biotechnology

authors： Chen MX,Zheng SX,Yang YN,Xu C,Liu JS,Yang WD,Chye ML,Li HY

更新日期：2014-03-20 00:00:00

abstract：:Pituitary tumor-transforming gene 1 (PTTG1), a proto-oncogene, is associated with tumor formation, proliferation and invasiveness. F-PBF(beta-TrCP), a fusion protein, was produced by replacing the WD40-repeat of F-box protein beta-TrCP with the PTTG1-binding factor (PBF) for targeted degradation of PTTG1. To evaluate ...

journal_title：Journal of biotechnology

authors： Mo Z,Zu X,Xie Z,Li W,Ning H,Jiang Y,Xu W

更新日期：2009-01-01 00:00:00

abstract：:A novel bacterial species was isolated from an industrial-scale biogas plant. The isolate Herbinix hemicellulosilytica T3/55(T) is able to degrade crystalline cellulose. Comparative 16S rRNA gene sequencing demonstrated that the isolate is closely related to environmental samples forming a hitherto unknown sub-cluster...

journal_title：Journal of biotechnology

authors： Koeck DE,Maus I,Wibberg D,Winkler A,Zverlov VV,Liebl W,Pühler A,Schwarz WH,Schlüter A

更新日期：2015-11-20 00:00:00

abstract：:Many recent epigenetic studies utilize the advantages of CRISPR/dCas9 based tools in linking certain epigenetic modification with gene expression regulation. Various multifactorial diseases often contain changed epigenetic signatures at many loci, so tools for simultaneously targeting different loci would significantl...

journal_title：Journal of biotechnology

authors： Josipović G,Zoldoš V,Vojta A

更新日期：2019-08-10 00:00:00

abstract：:The rare earth elements Nd, La, Ce at proper concentrations had positive effects on the cell growth of Cistanche deserticola and production of phenylethanoid glycosides (PeG). A mixture of rare earth elements (MRE, La(2)O(3):CeO(2):Pr(6)O(11):Sm(2)O(3)=255:175:3:1, mol/mol) showed the most remarkable effects. After 30...

journal_title：Journal of biotechnology

authors： Ouyang J,Wang X,Zhao B,Yuan X,Wang Y

更新日期：2003-04-24 00:00:00

abstract：:While phase I and phase II drug metabolites are important for drug development and toxicity studies, e.g. in the context of metabolites in safety testing (MIST), they are often not commercially available and their classical chemical synthesis can be cumbersome. Therefore, a biotechnological production of drug metaboli...

journal_title：Journal of biotechnology

authors： Naumann JM,Messinger J,Bureik M

更新日期：2010-10-01 00:00:00

abstract：:Herbaspirillum seropedicae is a nitrogen-fixing bacterium found in association with economically important gramineae. Regulation of nitrogen fixation involves the transcriptional activator NifA protein. The regulation of NifA protein and its truncated mutant proteins is described and compared with that of other nitrog...

journal_title：Journal of biotechnology

authors： Pedrosa FO,Benelli EM,Yates MG,Wassem R,Monteiro RA,Klassen G,Steffens MB,Souza EM,Chubatsu LS,Rigo LU

更新日期：2001-10-04 00:00:00

abstract：:The methylotropic yeast Pichia pastoris has demonstrated its suitability for large-scale production of recombinant proteins. As an eukaryotic organism P. pastoris presents a series of advantages at expression and processing of heterologous proteins when compared with Escherichia coli. In this work, P. pastoris has bee...

journal_title：Journal of biotechnology

authors： de la Cruz S,Alcocer M,Madrid R,García A,Martín R,González I,García T

更新日期：2016-06-10 00:00:00

abstract：:The synthesis of galactooligosaccharides (GOS) by whole cells of Bifidobacterium bifidum NCIMB 41171 was investigated by developing a set of mathematical models. These were second order polynomial equations, which described responses related to the production of GOS constituents, the selectivity of lactose conversion ...

journal_title：Journal of biotechnology

authors： Osman A,Tzortzis G,Rastall RA,Charalampopoulos D

更新日期：2010-10-01 00:00:00

abstract：:Threonine aldolase (TA) catalyzes a reversible reaction, in which threonine is decomposed into glycine and acetaldehyde. The same enzyme can be used to catalyze aldol reaction between glycine and a variety of aromatic and aliphatic aldehydes, thus creating various alpha-amino-alcohols. Therefore, TA is a very promisin...

journal_title：Journal of biotechnology

authors： Wieteska L,Ionov M,Szemraj J,Feller C,Kolinski A,Gront D

更新日期：2015-04-10 00:00:00