Draft genome sequence of Herbinix hemicellulosilytica T3/55 T, a new thermophilic cellulose degrading bacterium isolated from a thermophilic biogas reactor.

abstract：:Xylanase T-6 is a thermostable alkaline-tolerant enzyme that is produced by Bacillus stearothermophilus T-6. Xylanase T-6 was found to bleach pulp effectively at pH 9 and 65 degrees C and was used successfully on an industrial-scale mill trial. To facilitate the future characterization of the protein via X-ray analysi...

journal_title：Journal of biotechnology

authors： Lapidot A,Mechaly A,Shoham Y

更新日期：1996-11-15 00:00:00

abstract：:In earlier study from our group, cholera toxin B subunit had been expressed in tomato for developing a plant-based vaccine against cholera. In the present investigation, gene for accessory colonization factor (acf) subunit A, earlier reported to be essential for efficient colonization in the intestine, has been expres...

journal_title：Journal of biotechnology

authors： Sharma MK,Jani D,Thungapathra M,Gautam JK,Meena LS,Singh Y,Ghosh A,Tyagi AK,Sharma AK

更新日期：2008-05-20 00:00:00

abstract：:The action of three glycoside hydrolase family GH51 and one GH54 α-arabinofuranosidases (ABFs) was studied on polymeric arabinoxylan and arabinoxylooligosaccharides. The substrates covered all possible arabinofuranosyl (Araf) substituents, i.e., terminal and internal α(1→2) and α(1→3)-linked Araf monosubstitutions and...

journal_title：Journal of biotechnology

authors： Koutaniemi S,Tenkanen M

更新日期：2016-07-10 00:00:00

abstract：:Poly(methyl methacrylate-acrylic acid) microspheres were prepared and the acid groups were activated by using either carbodiimide (CDI) or thionyl chloride (SOCl2). alpha-Amylase was covalently bound on these activated microspheres. The properties of the immobilized enzyme were investigated and compared with those of ...

journal_title：Journal of biotechnology

authors： Aksoy S,Tumturk H,Hasirci N

更新日期：1998-02-05 00:00:00

abstract：:One of the major constraints to performing large-scale transfections of cultured mammalian cells for the transient expression of recombinant proteins is the production of large quantities of purified plasmid DNA. In this report partially purified plasmid DNA was prepared by a method that combines alkaline lysis of E. ...

journal_title：Journal of biotechnology

authors： Wright JL,Jordan M,Wurm FM

更新日期：2003-05-08 00:00:00

abstract：:Bioprocesses such as production of organic acids or acid hydrolysis of bioresources during biofuel production often suffer limitations due to microbial sensitivity under acidic conditions. Approaches for improving the acid tolerance of these microbes have mainly focused on using metabolic engineering tools. Here, we t...

journal_title：Journal of biotechnology

authors： Basak S,Geng H,Jiang R

更新日期：2014-03-10 00:00:00

abstract：:Steady state studies in a chemostat enable the control of microbial growth rate at defined reaction conditions. The effects of bacteriophage M13 infection on maximum growth rate of Escherichia coli JM109 were studied in parallel operated chemostats on a milliliter-scale to analyze the steady state kinetics of phage pr...

journal_title：Journal of biotechnology

authors： Kick B,Behler KL,Severin TS,Weuster-Botz D

更新日期：2017-09-20 00:00:00

abstract：:The global occurrence of prostate cancer with a range of patient outcome has prompted various investigators to explore novel molecular biomarkers that can precisely detect and track this type of cancer severity. Several studies suggest that micro-RNAs have emerged to act as a new largely unexplored class of biomarkers...

journal_title：Journal of biotechnology

authors： Wani S,Kaul D,Mavuduru RS,Kakkar N,Bhatia A

更新日期：2017-10-10 00:00:00

abstract：:Enterokinase, a two-chain duodenal serine protease, activates trypsinogen by removing its N-terminal propeptide. Due to a clean cut after the non-primed site recognition sequence, the enterokinase light chain is frequently employed in biotechnology to separate N-terminal affinity tags from target proteins with authent...

journal_title：Journal of biotechnology

authors： Skala W,Goettig P,Brandstetter H

更新日期：2013-12-01 00:00:00

abstract：:We previously showed that creation of a double strand DNA break (DSB) by expressing I-SceI in an engineered Trichoderma reesei (Hypocrea jecorina) strain containing a I-SceI recognition site improved transformation and homologous integration efficiencies. In this study, we further improved homologous integration frequ...

journal_title：Journal of biotechnology

authors： Ouedraogo JP,Arentshorst M,Nikolaev I,Barends S,Ram AF

更新日期：2016-03-20 00:00:00

abstract：:The simultaneous biosynthesis of lovastatin (mevinolinic acid) and (+)-geodin by Aspergillus terreus ATCC 20542 with regard to the medium composition, i.e. the concentrations of carbon and nitrogen source, was described in this paper. A. terreus is a lovastatin producer but the formation of lovastatin was accompanied ...

journal_title：Journal of biotechnology

authors： Bizukojc M,Ledakowicz S

更新日期：2007-12-01 00:00:00

abstract：:To study the metaproteome of a biogas-producing microbial community, fermentation samples were taken from an agricultural biogas plant for microbial cell and protein extraction and corresponding metagenome analyses. Based on metagenome sequence data, taxonomic community profiling was performed to elucidate the composi...

journal_title：Journal of biotechnology

authors： Ortseifen V,Stolze Y,Maus I,Sczyrba A,Bremges A,Albaum SP,Jaenicke S,Fracowiak J,Pühler A,Schlüter A

更新日期：2016-08-10 00:00:00

abstract：:Dilute-acid lignocellulosic hydrolyzate was successfully fermented to ethanol by encapsulated Saccharomyces cerevisiae at dilution rates up to 0.5h(-1). The hydrolyzate was so toxic that freely suspended yeast cells could ferment it continuously just up to dilution rate 0.1h(-1), where the cells lost 75% of their viab...

journal_title：Journal of biotechnology

authors： Talebnia F,Taherzadeh MJ

更新日期：2006-09-18 00:00:00

abstract：:Cupriavidus nantongensis X1 is a chlorpyrifos degrading bacterium, which was isolated from sludge collected at the drain outlet of a chlorpyrifos manufacture plant. It is the first time to report the complete genome sequence of C. nantongensis species, which has been reported as a novel species of Cupriavidus genus. I...

journal_title：Journal of biotechnology

authors： Fang LC,Chen YF,Zhou YL,Wang DS,Sun LN,Tang XY,Hua RM

更新日期：2016-06-10 00:00:00

abstract：:Physiologically relevant in vitro models can serve as biological analytical platforms for testing novel treatments and drug delivery systems. We describe the first steps in the development of a 3D human brain tumour co-culture model that includes the interplay between normal and tumour tissue along with nutrient gradi...

journal_title：Journal of biotechnology

authors： Ivanov DP,Parker TL,Walker DA,Alexander C,Ashford MB,Gellert PR,Garnett MC

更新日期：2015-07-10 00:00:00

abstract：:To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocy...

journal_title：Journal of biotechnology

authors： Zheng Y,Song X,Darby M,Liang Y,He L,Cai Z,Chen Q,Bi Y,Yang X,Xu J,Li Y,Sun Y,Lee RJ,Hou S

更新日期：2010-01-01 00:00:00

abstract：:Baculovirus vectors show promise as a novel tool for gene delivery into mammalian cells and gene transfer with wild-type baculovirus has been demonstrated both in vitro and in vivo. To study expression and intracellular trafficking of foreign viral membrane proteins in baculovirus-transduced mammalian cells, the envel...

journal_title：Journal of biotechnology

authors： Ojala K,Tikka PJ,Kautto L,Käpylä P,Marjomäki V,Oker-Blom C

更新日期：2004-10-19 00:00:00

abstract：:Different systems are being studied and used to prepare recombinant proteins for pharmaceutical use. The blood, and still more the milk, from transgenic animals appear a very attractive source of pharmaceuticals. The cells from these animals are expected to produce well-matured proteins in potentially huge amounts. Se...

journal_title：Journal of biotechnology

authors： Houdebine LM

更新日期：1994-05-31 00:00:00

abstract：:Enzymatic hydrolysis constitutes an attractive strategy for biorefining of abundant, low-cost agricultural by-products such as wheat bran and straw. However, to adopt such an approach, efficient enzymes are required, in particular xylanases. To promote heat-induced disorganization of the complex cell wall network in w...

journal_title：Journal of biotechnology

authors： Paës G,O'Donohue MJ

更新日期：2006-09-18 00:00:00

abstract：:Several green fluorescent protein (Gfp) mutants with increased cellular fluorescence compared to the wildtype protein have recently been generated. We have expressed and compared wildtype Gfp and mutants S65T, F100S/ M154T/V164A, F64L/S65T, and S65A/V68L/S72A under identical growth conditions in CHO and Saccharomyces ...

journal_title：Journal of biotechnology

authors： Natarajan A,Subramanian S,Srienc F

更新日期：1998-06-11 00:00:00

abstract：:Ebola virus disease outbreaks have repeatedly occurred on the African continent over the last decades, with more serious outbreaks in recent years. Being highly transmissible and associated to high fatality rates, it constitutes a serious threat to public health. Vaccination, however, may allow for efficient control o...

journal_title：Journal of biotechnology

authors： Kiesslich S,Vila-Chã Losa JP,Gélinas JF,Kamen AA

更新日期：2020-02-20 00:00:00

abstract：:Candida antarctica lipase B (CALB) has been employed as an efficient catalyst in the preparation of many flavor esters. A CALB-displaying yeast whole-cell biocatalyst could be an attractive alternative to commercial immobilized CALB because of its low-cost preparation and high enzymatic activity. We investigated the p...

journal_title：Journal of biotechnology

authors： Jin Z,Ntwali J,Han SY,Zheng SP,Lin Y

更新日期：2012-05-31 00:00:00

abstract：:Clostridium cellulovorans produces large extracellular enzyme complex, called cellulosomes. The diversity of the cellulosomal enzymes, which are secreted by C. cellulovorans that has been cultured on different carbon sources, such as Avicel, xylan, AXP (Avicel-xylan-pectin, 3:1:1) and cellobiose, was explored by two-d...

journal_title：Journal of biotechnology

authors： Cho W,Jeon SD,Shim HJ,Doi RH,Han SO

更新日期：2010-02-01 00:00:00

abstract：:The use of high capacity micron-sized non-porous magnetic metal chelator adsorbents for the direct recovery of a recombinant metal-binding protein from crude liquors is described. Selectivity and interaction strength of magnetic chelator particles were assessed using a set of native proteins with known behaviour towar...

journal_title：Journal of biotechnology

authors： O'Brien SM,Sloane RP,Thomas OR,Dunnill P

更新日期：1997-04-04 00:00:00

abstract：:Phytopathogenic fungi penetrate plants by breaking down the cuticular barrier with cutinase. Cutinases are extracellular hydrolytic enzymes that degrade cutin, a polyester composed of hydroxy and epoxy fatty acids. Until now, cutinase has been recognized by its ability to release labeled cutin monomers or by a non-spe...

journal_title：Journal of biotechnology

authors： Degani O,Salman H,Gepstein S,Dosoretz CG

更新日期：2006-02-10 00:00:00

abstract：:We report the complete genome sequence of Amycolatopsis japonica MG417-CF17(T) (=DSM 44213(T)) which was identified as the producer of (S,S)-N,N'-ethylenediaminedisuccinic acid during a screening for phospholipase C inhibitors. The genome of A. japonica MG417-CF17(T) consists of two replicons: the chromosome (8,961,31...

journal_title：Journal of biotechnology

authors： Stegmann E,Albersmeier A,Spohn M,Gert H,Weber T,Wohlleben W,Kalinowski J,Rückert C

更新日期：2014-11-10 00:00:00

abstract：:Pellet growth of Aspergillus terreus ATCC 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pel...

journal_title：Journal of biotechnology

authors： Casas López JL,Sánchez Pérez JA,Fernández Sevilla JM,Rodríguez Porcel EM,Chisti Y

更新日期：2005-03-02 00:00:00

abstract：:The hematopoietic system represents an interesting model for gene transfer protocols. Here, we have evaluated the efficiency of a gene transfer system using the polycationic compound SuperFect (Qiagen) and the K562 hematopoietic cell line. Transient and stable vectors carrying the enhanced green fluorescent protein (E...

journal_title：Journal of biotechnology

authors： Teixeira LA,Fricke CH,Bonorino CB,Bogo MR,Nardi NB

更新日期：2001-06-15 00:00:00

abstract：:Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedure of direct oligon...

journal_title：Journal of biotechnology

authors： Jensen MA,Akhras MS,Fukushima M,Pourmand N,Davis RW

更新日期：2013-09-20 00:00:00

abstract：:The use of recombinant proteins has increased greatly in recent years, as well as the techniques used for their purification. The selection of an efficient process to purify proteins is a major bottleneck found when trying to scale up results obtained in the laboratory to a large-scale industrial process. One of the m...

journal_title：Journal of biotechnology

authors： Vásquez-Alvarez E,Pinto JM

更新日期：2004-06-10 00:00:00