Preparation and characterization of folate-poly(ethylene glycol)-grafted-trimethylchitosan for intracellular transport of protein through folate receptor-mediated endocytosis.

Abstract:

:To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocyanate conjugated bovine serum albumin (FITC-BSA) were prepared under suitable weight ratio of copolymer to FITC-BSA by ionic interaction between the positively charged copolymers and the negatively charged FITC-BSA. Intracellular uptake of FITC-BSA was specifically enhanced in SKOV3 cells (folate receptor over-expressing cell line) through folate receptor-mediated endocytosis compared with A549 cells (folate receptor deficient cell line). Folate-PEG-g-TMC shows promise for intracellular transport of negatively charged therapeutic proteins into folate receptor over-expressing tumor cells.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Zheng Y,Song X,Darby M,Liang Y,He L,Cai Z,Chen Q,Bi Y,Yang X,Xu J,Li Y,Sun Y,Lee RJ,Hou S

doi

10.1016/j.jbiotec.2009.09.007

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

47-53

issue

1

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(09)00414-3

journal_volume

145

pub_type

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