Complete genome sequence of the actinobacterium Amycolatopsis japonica MG417-CF17(T) (=DSM 44213T) producing (S,S)-N,N'-ethylenediaminedisuccinic acid.

abstract：:Hepatocyte growth factor (HGF) is biosynthesized as a biologically inactive, single-chain form (pro-HGF). Its activation is associated with cleavage at Arg494-Val495 into a two-chain mature form composed of disulfide-linked alpha- and beta-chains. Because serum is a major source of HGF activator (the predominant serin...

journal_title：Journal of biotechnology

authors： Hayata D,Fukuta K,Matsumoto K,Adachi E,Hanada K,Adachi K,Nakamura T

更新日期：2008-02-29 00:00:00

abstract：:Two different approaches of matrix assisted refolding have been evaluated and compared to conventional refolding by dilution. Bovine alpha-lactalbumin was used for the studies as model protein. It was adsorbed under denaturing conditions on an ion exchange matrix and refolding was completed on the column prior to elut...

journal_title：Journal of biotechnology

authors： Machold C,Schlegl R,Buchinger W,Jungbauer A

更新日期：2005-04-20 00:00:00

abstract：:Production of biopharmaceuticals from mammalian cells requires generation of master, working and post-production cell banks of high quality under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to establishing production processes that are comp...

journal_title：Journal of biotechnology

authors： Fieder J,Wagner N,Grammatikos S,Hoffmann H,Kaufmann H,Otto RT

更新日期：2005-10-17 00:00:00

abstract：:MicroRNAs (miRNAs) are gaining recognition as essential regulators involved in many biological processes, and they are emerging as therapeutic targets for treating disease. Here, we introduce a method for effective delivery of anti-miRNA oligonucleotides (AMOs) using functionalized gold nanoparticles (AuNPs). To demon...

journal_title：Journal of biotechnology

authors： Kim JH,Yeom JH,Ko JJ,Han MS,Lee K,Na SY,Bae J

更新日期：2011-09-20 00:00:00

abstract：:Combinatorial library selections through the systematic evolution of ligands by exponential enrichment (SELEX) technique identify so-called nucleic acid aptamers that bind with high-affinity and specificity to a wide range of selected molecules. However, the modest chemical functionality of nucleic acids poses some li...

journal_title：Journal of biotechnology

authors： Kusser W

更新日期：2000-03-01 00:00:00

abstract：:Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treat...

journal_title：Journal of biotechnology

authors： Lee JS,Kim YJ,Kim CL,Lee GM

更新日期：2012-09-15 00:00:00

abstract：:Cell culture optimization is a labor-intensive process requiring a large number of experiments to be conducted under varying conditions. Here we describe a high-throughput bioreactor system that allows 12 mini stirred-tank bioreactors to be operated simultaneously. All bioreactors are monitored by low-cost minimally i...

journal_title：Journal of biotechnology

authors： Ge X,Hanson M,Shen H,Kostov Y,Brorson KA,Frey DD,Moreira AR,Rao G

更新日期：2006-04-10 00:00:00

abstract：:"CloneAssistant 1.0" is a stand-alone software compatible with the current Windows operating systems, which can automatically design cloning primers with full consideration of the sequence information of vectors and genes, cloning strategies, the principles of primer design, reading frames, position effects, and enzym...

journal_title：Journal of biotechnology

authors： Shao C,Meng Y,Lv S,Zhong W,Wang Z,Chen M

更新日期：2010-11-01 00:00:00

abstract：:Protein modifications by intricate cellular machineries often redesign the structure and function of existing proteins to impact biological networks. Disulfide bond formation between cysteine (Cys) pairs is one of the most common modifications found in extracellularly-destined proteins, key to maintaining protein stru...

journal_title：Journal of biotechnology

authors： Zhong X,He T,Prashad AS,Wang W,Cohen J,Ferguson D,Tam AS,Sousa E,Lin L,Tchistiakova L,Gatto S,D'Antona A,Luan YT,Ma W,Zollner R,Zhou J,Arve B,Somers W,Kriz R

更新日期：2017-04-20 00:00:00

abstract：:Linoleate 13-hydratase from Lactobacillus acidophilus LMG 11470 converted linoleic acid to hydroxyl fatty acid, which was identified as 13S-hydroxy-9(Z)-octadecenoic acid (13-HOD) by GC-MS and NMR. The expression of linoleate 13-hydratase gene in Escherichia coli was maximized by using pACYC plasmid and super optimal ...

journal_title：Journal of biotechnology

authors： Park JY,Lee SH,Kim KR,Park JB,Oh DK

更新日期：2015-08-20 00:00:00

abstract：:Penicillium purpurogenum secretes arabinofuranosidase to the growth medium. Highest levels of enzyme (1.0 U ml(-1)) are obtained when L-arabitol is used as carbon source, while 0.85 and 0.7 U ml(-1) are produced with sugar beet pulp and oat spelts xylan, respectively. By means of a zymogram, three bands with arabinofu...

journal_title：Journal of biotechnology

authors： De Ioannes P,Peirano A,Steiner J,Eyzaguirre J

更新日期：2000-01-21 00:00:00

abstract：:Consumers prefer biotechnological food products with high nutritional values and good flavors. Solid-state fermentation is a commonly used technique with a long history. In the present study, Myroides sp. ZB35 was used in solid-state fermentative production of aroma volatiles on a rice medium. Using the headspace soli...

journal_title：Journal of biotechnology

authors： Xiao Z,Liang W,Zhu X,Zhao JY

更新日期：2017-03-10 00:00:00

abstract：:Six bacterial strains with the capability of degrading textile dyes were isolated from sludge samples and mud lakes. Aeromonas hydrophila was selected and identified because it exhibited the greatest color removal from various dyes. Although A. hydrophila displayed good growth in aerobic or agitation culture (AGI cult...

journal_title：Journal of biotechnology

authors： Chen KC,Wu JY,Liou DJ,Hwang SC

更新日期：2003-02-27 00:00:00

abstract：:Stem borer resistant transgenic parental lines, involved in hybrid rice, were produced by Agrobacterium-mediated gene transfer method. Two pSB111 super-binary vectors containing modified cry1Ab/cry1Ac genes driven by maize ubiquitin promoter, and herbicide resistance gene bar driven by cauliflower mosaic virus 35S pro...

journal_title：Journal of biotechnology

authors： Ramesh S,Nagadhara D,Pasalu IC,Kumari AP,Sarma NP,Reddy VD,Rao KV

更新日期：2004-07-15 00:00:00

abstract：:An enzymatically-active fungal cellobiohydrolase I (CBH I) was first synthesized in a coupled reticulocyte lysate system lacking of glycosylation modification by the template DNA(Cbh1) in the presence of T7 RNA polymerase. The synthesized CBH I had the expected size (57 kDa) and catalyzed the substrate of p-nitropheny...

journal_title：Journal of biotechnology

authors： Wang J,Gao P

更新日期：2000-08-25 00:00:00

abstract：:Norovirus infections cause severe gastroenteritis in millions of people every year. Infection requires the recognition of histo-blood group antigens (HBGAs), but such interactions can be inhibited by human milk oligosaccharides (HMOs), which act as structurally-similar decoys. HMO supplements could help to prevent nor...

journal_title：Journal of biotechnology

authors： Derya SM,Spiegel H,Hanisch FG,Morozov V,Schroten H,Jennewein S,Parschat K

更新日期：2020-07-20 00:00:00

abstract：:A solid-phase method is described to create subclones, suitable for DNA sequencing, from lambda or cosmid libraries. The purified target DNA is sonicated and two linkers, with one oligonucleotide biotinylated in the 5'-end, are ligated to the ends of the fragments produced by sonication. After size separation, the fra...

journal_title：Journal of biotechnology

authors： Hultman T,Uhlén M

更新日期：1994-06-30 00:00:00

abstract：:A broad methanotrophic community consisting of 16 different operational taxonomic units (OTUs) was detected by particulate methane monooxygenase A (pmoA) gene analyses of reactor sludge samples obtained from an industrial biogas plant. Using a cloning-sequencing approach, 75% of the OTUs were affiliated to the group o...

journal_title：Journal of biotechnology

authors： May T,Polag D,Keppler F,Greule M,Müller L,König H

更新日期：2018-03-20 00:00:00

abstract：:Endoglucanases (endo-1,4-beta-D-glucan-4-glucanohydrolase, EC 3.2.1.4) are industrially important enzymes. In this study endoglucanase I (EGI or Cel7B) of the filamentous fungi Trichoderma reesei has been genetically engineered to investigate the influence of tryptophan rich peptide extensions (tags) on partitioning i...

journal_title：Journal of biotechnology

authors： Collén A,Ward M,Tjerneld F,Stålbrand H

更新日期：2001-05-04 00:00:00

abstract：:Oil sands tailings ponds store the waste slurry generated by extracting bitumen from surface-mined oil (tar) sands ores. The ponds support diverse microbial communities involved in element cycling, greenhouse gas production, and hydrocarbon biodegradation that influence pond management and their environmental footprin...

journal_title：Journal of biotechnology

authors： Wilson SL,Li C,Ramos-Padrón E,Nesbø C,Soh J,Sensen CW,Voordouw G,Foght J,Gieg LM

更新日期：2016-10-10 00:00:00

abstract：:We previously developed a novel one-step biotransformation process for the production of α-ketoglutarate (α-KG) from L-glutamic acid by a Bacillus subtilis whole-cell biocatalyst expressing an L-amino acid deaminase (pm1) of Proteus mirabilis. However, the biotransformation efficiency of this process was low owing to ...

journal_title：Journal of biotechnology

authors： Hossain GS,Li J,Shin HD,Liu L,Wang M,Du G,Chen J

更新日期：2014-10-10 00:00:00

abstract：:Deployment of the elongated flexuous virions of Turnip mosaic virus (TuMV), a potyvirus, for peptide display on their external surface has been previously reported by us. Nonetheless, both in TuMV and other potyviruses some peptides hinder the ability of the virus to infect host plants. We found that a peptide derived...

journal_title：Journal of biotechnology

authors： González-Gamboa I,Manrique P,Sánchez F,Ponz F

更新日期：2017-07-20 00:00:00

abstract：:siRNA based therapeutics have become the next frontier in molecular medicine. Though exosomes emerge as a promising drug delivery vehicle for siRNAs, significant hurdle remains in finding safe and effective loading methods. Traditional methods of loading exogenous siRNAs in exosomes are marked by certain limitations l...

journal_title：Journal of biotechnology

authors： Shandilya S,Rani P,Onteru SK,Singh D

更新日期：2020-07-20 00:00:00

abstract：:The stability, copy number, and gene expression of the pBR322 plasmid containing the par-locus under prolonged cultivation were studied. In the initial stage of the experiment it was observed that the par-locus had a stabilization effect on plasmid maintenance. This observation was consistent with previously reported ...

journal_title：Journal of biotechnology

authors： Weber AE,Yu P,San KY

更新日期：1991-04-01 00:00:00

abstract：:Ubiquitination is one of the most significant posttranslational modifications (PTMs). To evaluate the ability of an insect cell-free protein synthesis system to carry out ubiquitin (Ub) conjugation to in vitro translated proteins, poly-Ub chain formation was studied in an insect cell-free protein synthesis system. Pol...

journal_title：Journal of biotechnology

authors： Suzuki T,Ezure T,Ando E,Nishimura O,Utsumi T,Tsunasawa S

更新日期：2010-01-01 00:00:00

abstract：:AL (SpA A domain-PpL B3 domain), LD5 (PpL B3 domain-SpA D domain-PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L-D-L) and LD3 (PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L) are novel evolved Ig binding molecules (NEIBMs) derived from the in vitro molecular evolution of combinatorial phage libraries displaying rando...

journal_title：Journal of biotechnology

authors： He T,Ding YY,Feng JJ,Chen QL,Zhu HM,Peng H,Rui B,Li XY,Cao MM,Pan W

更新日期：2014-08-20 00:00:00

abstract：:Bacillus velezensis G341 can suppress plant pathogens by producing antagonistic active compounds including bacillomycin D, fengycin, and (oxy) difficidin. The complete genome sequence of this bacterium was characterized by one circular chromosome of 4,009,746bp with 3953 open reading frames. The genome contained 36 ps...

journal_title：Journal of biotechnology

authors： Lee HH,Park J,Lim JY,Kim H,Choi GJ,Kim JC,Seo YS

更新日期：2015-10-10 00:00:00

abstract：:Owing to its dual impact on tissue engineering (neovascularization of tissue implants) and cancer treatment (prevention of tumor-induced vascularization), management and elucidation of vascularization phenomena remain clinical priorities. Using a variety of primary human cells and (neoplastic) cell lines assembled in ...

journal_title：Journal of biotechnology

authors： Kelm JM,Diaz Sanchez-Bustamante C,Ehler E,Hoerstrup SP,Djonov V,Ittner L,Fussenegger M

更新日期：2005-08-04 00:00:00

abstract：:Delivery of macromolecules into the cytosolic space of eukaryotic cells is a pressing challenge in biopharmaceutics. Macromolecules are often encapsulated into liposomes for protection and improved distribution, but the their size often induces endocytosis of the vehicle at the target site, leading to degradation of t...

journal_title：Journal of biotechnology

authors： Walls ZF,Goodell S,Andrews CD,Mathis J,Lee KD

更新日期：2013-04-15 00:00:00

abstract：:The enzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature of β-carboline (βC) alkaloid biosynthesis. NscbB is a rare microbial PSase discovered from a cryptic β-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplan...

journal_title：Journal of biotechnology

authors： Chen Q,Zhang S,Xie Y

更新日期：2018-09-10 00:00:00