Abstract:
:The potential of electric current-mediated delivery technology to enhance DNA delivery and DNA vaccine potency was evaluated. Higher levels of reporter gene expression were observed in muscle cells of mice inoculated with luciferase or beta-galactosidase DNA followed by the application of electrical current, compared with DNA injected with no current. Similarly, substantially higher levels of immune responses (up to 20-fold) were demonstrated in mice vaccinated with HIV gag DNA and electric current. These enhanced responses were observed after one or two inoculations, and were maintained for at least 12 weeks. Therefore, the present studies demonstrate the utility of electroporation for enhancement of DNA vaccine potency in animals.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Selby M,Goldbeck C,Pertile T,Walsh R,Ulmer Jdoi
10.1016/s0168-1656(00)00308-4subject
Has Abstractpub_date
2000-09-29 00:00:00pages
147-52issue
1-2eissn
0168-1656issn
1873-4863pii
S0168-1656(00)00308-4journal_volume
83pub_type
杂志文章abstract::The keto ester reductase from Candida glabrata, designated as CgKR1, is a highly versatile biocatalyst with a broad substrate spectrum. Its substrate preference was altered by rational design of the active pocket for bioreduction of aromatic α-keto esters in our previous work. However, its practical application is sti...
journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:1995-02-21 00:00:00
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
pub_type: 杂志文章
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更新日期:2016-07-10 00:00:00
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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journal_title:Journal of biotechnology
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pub_type: 杂志文章,评审
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journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
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journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
doi:10.1016/0168-1656(92)90064-g
更新日期:1992-06-01 00:00:00