Antitumor effect of F-PBF(beta-TrCP)-induced targeted PTTG1 degradation in HeLa cells.

Abstract:

:Pituitary tumor-transforming gene 1 (PTTG1), a proto-oncogene, is associated with tumor formation, proliferation and invasiveness. F-PBF(beta-TrCP), a fusion protein, was produced by replacing the WD40-repeat of F-box protein beta-TrCP with the PTTG1-binding factor (PBF) for targeted degradation of PTTG1. To evaluate the function of F-PBF(beta-TrCP), PTTG1-EGFP fusion protein was constructed. Our results showed that F-PBF(beta-TrCP) can both degrade exogenous PTTG1-EGFP fusion protein in COS-7 cells and endogenous PTTG1 protein in HeLa cells and the targeted PTTG1 knock down resulted in bFGF mRNA level down-regulation and inhibition of proliferation and clonogenicity in HeLa cells. In conclusion, targeted degradation of PTTG1 by F-PBF(beta-TrCP) has antitumor activity in vitro in HeLa cells. These results suggest that F-PBF(beta-TrCP) could be used for cancer treatment by targeted degradation of PTTG1.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Mo Z,Zu X,Xie Z,Li W,Ning H,Jiang Y,Xu W

doi

10.1016/j.jbiotec.2008.09.004

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

6-11

issue

1

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(08)02377-8

journal_volume

139

pub_type

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