Antitumor effect of F-PBF(beta-TrCP)-induced targeted PTTG1 degradation in HeLa cells.


:Pituitary tumor-transforming gene 1 (PTTG1), a proto-oncogene, is associated with tumor formation, proliferation and invasiveness. F-PBF(beta-TrCP), a fusion protein, was produced by replacing the WD40-repeat of F-box protein beta-TrCP with the PTTG1-binding factor (PBF) for targeted degradation of PTTG1. To evaluate the function of F-PBF(beta-TrCP), PTTG1-EGFP fusion protein was constructed. Our results showed that F-PBF(beta-TrCP) can both degrade exogenous PTTG1-EGFP fusion protein in COS-7 cells and endogenous PTTG1 protein in HeLa cells and the targeted PTTG1 knock down resulted in bFGF mRNA level down-regulation and inhibition of proliferation and clonogenicity in HeLa cells. In conclusion, targeted degradation of PTTG1 by F-PBF(beta-TrCP) has antitumor activity in vitro in HeLa cells. These results suggest that F-PBF(beta-TrCP) could be used for cancer treatment by targeted degradation of PTTG1.


J Biotechnol


Journal of biotechnology


Mo Z,Zu X,Xie Z,Li W,Ning H,Jiang Y,Xu W




Has Abstract


2009-01-01 00:00:00














  • Functional genomic analysis of the Bacillus subtilis Tat pathway for protein secretion.

    abstract::Protein secretion from Bacillus species is a major industrial production tool with a market of over $1 billion per year. However, standard export technologies, based on the well-characterised general secretory (Sec) pathway, are frequently inapplicable for the production of proteins. The recently discovered twin-argin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: van Dijl JM,Braun PG,Robinson C,Quax WJ,Antelmann H,Hecker M,Müller J,Tjalsma H,Bron S,Jongbloed JD

    更新日期:2002-09-25 00:00:00

  • Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde.

    abstract::Mutants of transketolase (TK) with improved substrate specificity towards the non-natural aliphatic aldehyde substrate propionaldehyde have been obtained by directed evolution. We used the same active-site targeted saturation mutagenesis libraries from which we previously identified mutants with improved activity towa...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hibbert EG,Senussi T,Smith ME,Costelloe SJ,Ward JM,Hailes HC,Dalby PA

    更新日期:2008-04-30 00:00:00

  • Studies on kinetic parameters and stability of aminoacylase in non-conventional media.

    abstract::Catalytic properties and conformational stability of aminoacylase (N-acylamino acid amidohydrolase, EC were studied in water-N,N-dimethylformamide (DMF) and water-dioxane solvent mixtures. Beside the prompt inhibition the solvents caused further inactivation during incubations. In the presence of 5% DMF cont...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Boross L,Kosáry J,Stefanovits-Bányai E,Sisak C,Szajáni B

    更新日期:1998-11-18 00:00:00

  • Metabolic flux screening of Saccharomyces cerevisiae single knockout strains on glucose and galactose supports elucidation of gene function.

    abstract::New methods for an extended physiological characterization of yeast at a microtiter plate scale were applied to 27 deletion mutants of Saccharomyces cerevisiae cultivated on glucose and galactose as sole carbon sources. In this way, specific growth rates, specific rates of glucose consumption and ethanol production we...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Velagapudi VR,Wittmann C,Schneider K,Heinzle E

    更新日期:2007-12-01 00:00:00

  • Generation of engineered recombinant hepatocyte growth factor cleaved and activated by Genenase I.

    abstract::Hepatocyte growth factor (HGF) is biosynthesized as a biologically inactive, single-chain form (pro-HGF). Its activation is associated with cleavage at Arg494-Val495 into a two-chain mature form composed of disulfide-linked alpha- and beta-chains. Because serum is a major source of HGF activator (the predominant serin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hayata D,Fukuta K,Matsumoto K,Adachi E,Hanada K,Adachi K,Nakamura T

    更新日期:2008-02-29 00:00:00

  • Enzymatic in situ saccharification of sugarcane bagasse pretreated with low loading of alkalic salts Na2SO3/Na3PO4 by autoclaving.

    abstract::Sugarcane bagasse (SCB) is an abundant, renewable and inexpensive agricultural byproduct for the production of biofuel and other biobased products. To effectively saccharify SCB with cellulases, combination with dilute alkali salts Na2SO3/Na3PO4 (0.4% Na3PO4, 0.03% Na2SO3) at 7.5% sulfidity and hot water (DASHW) in "o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Jiang CX,He YC,Chong GG,Di JH,Tang YJ,Ma CL

    更新日期:2017-10-10 00:00:00

  • High cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactors.

    abstract::This study demonstrates the applicability of pressurized stirred tank bioreactors for oxygen transfer enhancement in aerobic cultivation processes. The specific power input and the reactor pressure was employed as process variable. As model organism Escherichia coli, Arxula adeninivorans, Saccharomyces cerevisiae and ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Knoll A,Bartsch S,Husemann B,Engel P,Schroer K,Ribeiro B,Stöckmann C,Seletzky J,Büchs J

    更新日期:2007-10-31 00:00:00

  • Biosynthesis of 4-vinylguaiacol from crude ferulic acid by Bacillus licheniformis DLF-17056.

    abstract::4-vinylguaiacol, a kind of volatile phenolic compound with tobacco flavor, is widely used as a component of edible flavor and intermediate of medicine. Ferulic acid is usually used as substrate for the biosynthesis of 4-vinylguaiacol. However, the price of ferulic acid is high, leading to high production cost. In this...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Sun LH,Lv SW,Yu F,Li SN,He LY

    更新日期:2018-09-10 00:00:00

  • Monitoring Chinese hamster ovary cell culture by the analysis of glucose and lactate metabolism.

    abstract::Monitoring cell growth is crucial to the success of an animal cell culture process that can be accomplished by a variety of direct or indirect methodologies. Glucose is a major carbon and energy source for cultured mammalian cells in most cases, but glycolytic metabolism often results in the accumulation of lactate. G...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Tsao YS,Cardoso AG,Condon RG,Voloch M,Lio P,Lagos JC,Kearns BG,Liu Z

    更新日期:2005-08-22 00:00:00

  • Enzymatic synthesis of beta-lactam antibiotics via direct condensation.

    abstract::In this paper, the feasibility of precipitation driven synthesis of acidic and zwitterionic beta-lactam antibiotics is studied. As an example of the first type, penicillin G was produced in good yield (160 mmol kg(-1)) directly from the free acid and amine aqueous substrate suspension, where the synthesis product prec...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ulijn RV,De Martin L,Halling PJ,Moore BD,Janssen AE

    更新日期:2002-11-13 00:00:00

  • Improvement of a d-biotin-hyperproducing recombinant strain of Serratia marcescens.

    abstract::We previously reported that a recombinant strain, SB412(pLGM304), was constructed from acidomycin-resistant mutants of Serratia marcescens and produced 200 mg of d-biotin per liter of a medium containing sucrose and urea (Sakurai et al., 1993a, b). In the present work, we intended to improve the d-biotin production. B...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Sakurai N,Imai Y,Masuda M,Komatsubara S,Tosa T

    更新日期:1994-07-29 00:00:00

  • Differential induction of autophagy in caspase-3/7 down-regulating and Bcl-2 overexpressing recombinant CHO cells subjected to sodium butyrate treatment.

    abstract::Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treat...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lee JS,Kim YJ,Kim CL,Lee GM

    更新日期:2012-09-15 00:00:00

  • Rapid and quantitative analysis of recombinant protein expression using pyrolysis mass spectrometry and artificial neural networks: application to mammalian cytochrome b5 in Escherichia coli.

    abstract::Recombinant Escherichia coli clones encoding between 0 and 6 copies of the mammalian cytochrome b5 gene were subjected to pyrolysis mass spectrometry (PyMS). To deconvolute the pyrolysis mass spectra so as to obtain quantitative information on the amount of cytochrome b5 produced fully-interconnected feedforward artif...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Goodacre R,Karim A,Kaderbhai MA,Kell DB

    更新日期:1994-05-15 00:00:00

  • Gene expression analysis using a modified HiCEP method applicable to prokaryotes: a study of the response of Rhodococcus to isoniazid and ethambutol.

    abstract::We established a novel method to analyze the gene expression of prokaryotes by modifying and optimizing the HiCEP (high coverage gene expression analysis) method, which was originally developed for eukaryotic gene expression profiling. Following the removal of abundant rRNA, the mRNA of prokaryotic cells was enzymatic...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Mitani Y,Suzuki K,Kondo K,Okumura K,Tamura T

    更新日期:2006-05-29 00:00:00

  • Reprint of Design of synthetic microbial communities for biotechnological production processes.

    abstract::In their natural habitats microorganisms live in multi-species communities, in which the community members exhibit complex metabolic interactions. In contrast, biotechnological production processes catalyzed by microorganisms are usually carried out with single strains in pure cultures. A number of production processe...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Jagmann N,Philipp B

    更新日期:2014-12-20 00:00:00

  • Measurement of intracellular pH in cultured cells by flow cytometry with BCECF-AM.

    abstract::This study evaluates the suitability of flow cytometry with the fluorochrome BCECF for measuring the intracellular pH (pHi) of cultured cells, and monitors the changes in pHi in murine hybridoma in batch culture and chick embryo fibroblast in monolayer culture (5th passage). The technique produced highly reproducible,...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Franck P,Petitipain N,Cherlet M,Dardennes M,Maachi F,Schutz B,Poisson L,Nabet P

    更新日期:1996-05-15 00:00:00

  • Optimization of a high-cell-density polyethylenimine transfection method for rapid protein production in CHO-EBNA1 cells.

    abstract::For pre-clinical evaluation of biotherapeutic candidates, protein production by transient gene expression (TGE) in Chinese Hamster Ovary (CHO) cells offers important advantages, including the capability of rapidly and cost-effectively generating recombinant proteins that are highly similar to those produced in stable ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Stuible M,Burlacu A,Perret S,Brochu D,Paul-Roc B,Baardsnes J,Loignon M,Grazzini E,Durocher Y

    更新日期:2018-09-10 00:00:00

  • Optimisation of aqueous two-phase extraction of human antibodies.

    abstract::The purification of human antibodies in an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) 6000 and phosphate was optimised by surface response methodology. A central composite design was used to evaluate the influence of phosphate, PEG and NaCl concentration and of the pH on the purity and extra...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Azevedo AM,Rosa PA,Ferreira IF,Aires-Barros MR

    更新日期:2007-10-31 00:00:00

  • Enzyme cascade for biocatalytic deracemization of D,L-phosphinothricin.

    abstract::Deracemization of D,L-phosphinothricin (D,L-PPT) is one of the most promising routes for preparation of optically pure L-PPT. In this work, an efficient multi-enzyme redox cascade was developed for deracemization ofPPT, which includes oxidative reaction and reductive reaction. The oxidative reaction catalyzing oxidati...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Cao CH,Gong H,Dong Y,Li JM,Cheng F,Xue YP,Zheng YG

    更新日期:2021-01-10 00:00:00

  • The bacterial ParA-ParB partitioning proteins.

    abstract::A pair of genes designated parA and parB are encoded by many low copy number plasmids and bacterial chromosomes. They work with one or more cis-acting sites termed centromere-like sequences to ensure better than random predivisional partitioning of the DNA molecule that encodes them. The centromere-like sequences nucl...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Bignell C,Thomas CM

    更新日期:2001-09-13 00:00:00

  • DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound.

    abstract::Magnetic particles produced by magnetic bacteria have been used to carry out magnetic separation of DNA. Separation was achieved using magnetite coated with 3-aminopropyltriethoxysilane, N-(trimethoxy-silylpropyl) isothiouronium chloride or 3-[2-(2-aminoethyl)-ethylamino]-propyltrimethoxysilane (AEEA). The DNA binding...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yoza B,Matsumoto M,Matsunaga T

    更新日期:2002-04-11 00:00:00

  • High performance flow injection analysis of recombinant protein G.

    abstract::Chromatographic discs were investigated for their potential to substitute for the hitherto used cartridges in heterogeneous flow injection analysis. Originally designed for fast high performance liquid chromatography (HPLC) of biopolymers, the discs combine reliability with speed and resolution. This together with the...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Hagedorn J,Kasper C,Freitag R,Tennikova T

    更新日期:1999-03-26 00:00:00

  • Innovative use of Mucuna monosperma (Wight) callus cultures for continuous production of melanin by using statistically optimized biotransformation medium.

    abstract::Melanins are predominantly indolic polymers which are having extensive applications in cosmetics, agriculture and medicine. In the present study, optimization of nutritional parameters influencing melanin production by Mucuna monosperma callus cultures was attempted using the response surface methodology (RSM). Standa...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Inamdar S,Joshi S,Bapat V,Jadhav J

    更新日期:2014-01-20 00:00:00

  • Amperometric enzyme biosensors based on optimised electron-transfer pathways and non-manual immobilisation procedures.

    abstract::Development of reagentless biosensors implies the tight and functional immobilisation of biological recognition elements on transducer surfaces. Specifically, in the case of amperometric enzyme electrodes, electron-transfer pathways between the immobilised redox protein and the electrode surface have to be established...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Schuhmann W

    更新日期:2002-02-01 00:00:00

  • Lrp of Corynebacterium glutamicum controls expression of the brnFE operon encoding the export system for L-methionine and branched-chain amino acids.

    abstract::Corynebacterium glutamicum possesses export systems for various amino acids including BrnFE, a two-component export system for L-methionine and the branched-chain amino acids L-valine, L-isoleucine and L-leucine. A gene for a putative transcriptional regulator of the Lrp family is transcribed divergently to the brnFE ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lange C,Mustafi N,Frunzke J,Kennerknecht N,Wessel M,Bott M,Wendisch VF

    更新日期:2012-04-30 00:00:00

  • Expression of the Streptococcus agalactiae virulence-associated protease CspA in a soluble, active form utilizing the Gram-positive host, Lactococcus lactis.

    abstract::The heterologous expression of streptococcal genes in common Gram-negative hosts may be complicated by low-level expression, toxicity to the host, formation of inclusion bodies, and mislocalization of the encoded proteins. Biochemical study of the Streptococcus agalactiae virulence-associated cell-envelope protease (C...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Shelver D,Bryan JD

    更新日期:2008-09-10 00:00:00

  • Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolates.

    abstract::Acinetobacter baumannii has turned into an important threat in nosocomial outbreak infections and multidrug resistance leading to high mortality rates in the 21st century. In recent years its mortality has increased by 15% which in part could be due to lack of a rapid and sensitive diagnostic test. In this work we int...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Rasoulinejad S,Gargari SLM

    更新日期:2016-08-10 00:00:00

  • Clavibacter michiganensis subsp. michiganensis: first steps in the understanding of virulence of a Gram-positive phytopathogenic bacterium.

    abstract::Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete. It infects tomato, spreads through the xylem and causes bacterial wilt and canker. The wild-type strain NCPPB382 carries two plasmids, pCM1 and pCM2. The cured plasmid-free derivative CMM100 is still able to colonize tomato, but no dise...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Gartemann KH,Kirchner O,Engemann J,Gräfen I,Eichenlaub R,Burger A

    更新日期:2003-12-19 00:00:00

  • Effects of the hydrodynamic environment and shear protectants on survival of erythrocytes in suspension.

    abstract::Survival of media-suspended porcine erythrocytes exposed to various hydrodynamic environments was investigated with and without such shear protectant additives as bovine serum albumin, dextran and the non-ionic surfactant Pluronic F68. Erythrocytes provided a model cell population with cells of a uniform size, metabol...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zhang Z,Chisti Y,Moo-Young M

    更新日期:1995-11-21 00:00:00

  • Enoate reductases from non conventional yeasts: bioconversion, cloning, and functional expression in Saccharomyces cerevisiae.

    abstract::Old yellow enzymes (OYEs, EC are flavin-dependent oxidoreductases that catalyze the stereoselective trans-hydrogenation of the double bond, representing a promising alternative to metal-based catalysis. Bioconversion of ketoisophorone (KIP) by 28 non-conventional yeasts belonging to 16 different species was ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Raimondi S,Romano D,Amaretti A,Molinari F,Rossi M

    更新日期:2011-12-20 00:00:00