Abstract:
:The expression of heterologous proteins in microorganisms using genetic recombination is still the high point in the development and exploitation of modern biotechnology. People can produce bioactive proteins from relatively cheap culture medium instead of expensive extraction. Host cell systems for the expression of heterologous genes are generally prokaryotic or eukaryotic systems, both of which have inherent advantages and drawbacks. An optimal expression system can be selected only if the productivity, bioactivity, purpose, and physicochemical characteristics of the interest protein are taken into consideration, together with the cost, convenience and safety of the system itself. Here, we concisely review the most frequently used prokaryotic, yeast, insect and mammalian expression systems, as well as expression in eukaryote individuals. The merits and demerits of these systems are discussed.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Yin J,Li G,Ren X,Herrler Gdoi
10.1016/j.jbiotec.2006.07.012subject
Has Abstractpub_date
2007-01-10 00:00:00pages
335-47issue
3eissn
0168-1656issn
1873-4863pii
S0168-1656(06)00623-7journal_volume
127pub_type
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