Abstract：
:Ectoine and 5-hydroxyectoine belong to the family of compatible solutes which are known to mainly contribute to the adaptation of the cell to osmotic stress by mediation of a constant turgor. In addition the cell's essential functions are maintained under stress conditions like high salinity, heat or aridity stress. Hansenula polymorpha was engineered to catalyze the transformation of monomeric substrates to 5-hydroxyectoine. For this purpose four genes encoding the enzymes of the 5-hydroxyectoine biosynthesis pathway of Halomonas elongata, EctA, EctB, EctC, and EctD, were inserted into the genome of H. polymorpha. Subsequently the syntheses of ectoine and 5-hydroxyectoine were analyzed and optimized. We showed that H. polymorpha is a suitable system for recombinant 5-hydroxyectoine synthesis in gram per liter scale (2.8 g L⁻¹ culture supernatant, 365 μmol/g dcw) in which almost 100% conversion of ectoine to 5-hydroxyectoine without necessity of high salinity were achieved.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Eilert E,Kranz A,Hollenberg CP,Piontek M,Suckow Mdoi
10.1016/j.jbiotec.2013.02.005subject
Has Abstractpub_date
2013-08-20 00:00:00pages
85-93issue
2eissn
0168-1656issn
1873-4863pii
S0168-1656(13)00069-2journal_volume
167pub_type
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