Effects of rare earth elements on the growth of Cistanche deserticola cells and the production of phenylethanoid glycosides.

abstract：:A microcarrier culture system was established for a large-scale production of functional human endothelial cells. It has been difficult to cultivate human endothelial cells in large quantities for the reasons that specific growth factor and extracellular matrix are required for the survival and proliferation of the ce...

journal_title：Journal of biotechnology

authors： Tashiro S,Tsumoto K,Sano E

更新日期：2012-08-31 00:00:00

abstract：:One potential reason for the enhancement of immune responses to DNA vaccines following electroporation is increased gene expression. However, the inflammatory response and accompanying cellular infiltration stimulated by electroporation may also be essential for enhancing immune responses to DNA vaccines. These parame...

journal_title：Journal of biotechnology

authors： Babiuk S,Baca-Estrada ME,Foldvari M,Middleton DM,Rabussay D,Widera G,Babiuk LA

更新日期：2004-05-13 00:00:00

abstract：:Planococcus rifietoensis M8(T) (=DSM 15069(T)=ATCC BAA-790(T)) is a halotolerant bacterium with potential plant growth promoting properties isolated from an algal mat collected from a sulfurous spring in Campania (Italy). This paper presents the first complete genome of P. rifietoensis M8(T). Genes coding for various ...

journal_title：Journal of biotechnology

authors： See-Too WS,Convey P,Pearce DA,Lim YL,Ee R,Yin WF,Chan KG

更新日期：2016-03-10 00:00:00

abstract：:Soil salinity is one of the major environmental factors, influencing agricultural productivity of crops. As a non-edible and ideal oilseed crop, castor (Ricinus communis L.) has great industrial value in biofuel, but molecular mechanisms of salt stress regulation are still unknown. In this study, the differentially ex...

journal_title：Journal of biotechnology

authors： Lei P,Liu Z,Hu Y,Kim H,Liu S,Liu J,Xu L,Li J,Zhao Y,Yu Z,Qu Y,Huang F,Meng F

更新日期：2021-01-06 00:00:00

abstract：:Fluorescence resonance energy transfer studies allow to determine global shape properties of nucleic acids and nucleoprotein complexes. In many DNA-protein complexes, the DNA is more or less bent and the degree of bending can be obtained by FRET. For example, the DNA in complex with the integration host factor (IHF) i...

journal_title：Journal of biotechnology

authors： Lorenz M,Hillisch A,Diekmann S

更新日期：2002-01-01 00:00:00

abstract：:Multiple phage-peptide constructs, where the peptides mimic sperm epitopes that bind to zona pellucida (ZP) proteins, were generated via selection from a phage display library using a novel approach. Selections were designed to allow for identification of ZP-binding phage clones with potential species-specific propert...

journal_title：Journal of biotechnology

authors： Samoylova TI,Cochran AM,Samoylov AM,Schemera B,Breiteneicher AH,Ditchkoff SS,Petrenko VA,Cox NR

更新日期：2012-12-31 00:00:00

abstract：:A pair of genes designated parA and parB are encoded by many low copy number plasmids and bacterial chromosomes. They work with one or more cis-acting sites termed centromere-like sequences to ensure better than random predivisional partitioning of the DNA molecule that encodes them. The centromere-like sequences nucl...

journal_title：Journal of biotechnology

authors： Bignell C,Thomas CM

更新日期：2001-09-13 00:00:00

abstract：:We developed an efficient chimeric promoter, MUASMSCP, with enhanced activity and salicylic acid (SA)/abscisic acid (ABA) inducibility, incorporating the upstream activation sequence (UAS) of Mirabilis mosaic virus full-length transcript (MUAS, -297 to -38) to the 5' end of Mirabilis mosaic virus sub-genomic transcrip...

journal_title：Journal of biotechnology

authors： Acharya S,Sengupta S,Patro S,Purohit S,Samal SK,Maiti IB,Dey N

更新日期：2014-01-01 00:00:00

abstract：:A D10A mutation was introduced at the 5'-3' exonuclease domain of Streptococcus pneumoniae DNA polymerase I by site directed mutagenesis of the polA gene. Introduction of the mutation resulted in a drastic decrease of the 5'-3' exonucleolytic activity present in the wild-type enzyme. Moreover, the mutation at the D10 ...

journal_title：Journal of biotechnology

authors： Amblar M,Sagner G,López P

更新日期：1998-07-30 00:00:00

abstract：:A two-stage culture system was established for the production of astaxanthin from Haematococcus pluvialis. In a first stage green vegetative cells were produced in semicontinuous cultures maintained with daily renewal rates between 10 and 40%. The steady-state cell density decreased with increasing renewal rates. High...

journal_title：Journal of biotechnology

authors： Fábregas J,Otero A,Maseda A,Domínguez A

更新日期：2001-07-26 00:00:00

abstract：:Site selective chemical modification is a preferred method, employed to prolong the circulation half-life of biopharmaceuticals. Cysteines have been used as attachment point for such modification, however, to be susceptible for chemical modification the involved thiol must be in its reduced form. Proteins often contai...

journal_title：Journal of biotechnology

authors： Jespersen GR,Matthiesen F,Pedersen AK,Andersen HS,Kirsebom H,Nielsen AL

更新日期：2014-03-10 00:00:00

abstract：:Izumoring is a new concept for the bioproduction of all hexose sugars - 16 aldohexoses, eight ketohexoses and 10 hexitols -- using enzymatic and microbiological reactions. The biocatalysts necessary for the strategy were (i) d-tagatose 3-epimerase [which epimerizes all ketohexoses at C-3 of the sugar], (ii) polyol deh...

journal_title：Journal of biotechnology

authors： Izumori K

更新日期：2006-08-05 00:00:00

abstract：:A new and simple method was devised to obtain immunoglobulin VH genes directly from primary cultured B cells specific for a short peptide. Peptide-specific B cells were separated from splenocytes of peptide-immunized BALB/c mice with antigen-coated magnetic beads, and were cloned by a limitedly diluted culture in the ...

journal_title：Journal of biotechnology

authors： Takahashi Y,Ametani A,Totsuka M,Kaminogawa S

更新日期：1996-08-20 00:00:00

abstract：:A solid-phase method is described to create subclones, suitable for DNA sequencing, from lambda or cosmid libraries. The purified target DNA is sonicated and two linkers, with one oligonucleotide biotinylated in the 5'-end, are ligated to the ends of the fragments produced by sonication. After size separation, the fra...

journal_title：Journal of biotechnology

authors： Hultman T,Uhlén M

更新日期：1994-06-30 00:00:00

abstract：:A simple and accurate analysis system for the estimation of recombination efficiency in vivo using fluorescence-activated cell sorting (FACS) was designed and was subsequently used to compare the efficiency of recombination related to different spacer mutants. F(3) and F(5) mutant sequences were used for Flpe-mediated...

journal_title：Journal of biotechnology

authors： Kim MS,Kim WH,Lee GM

更新日期：2007-01-10 00:00:00

abstract：:Plant virus vectors provide an attractive biotechnological tool for the transient expression of foreign genes in whole plants. As yet there has been no use of recombinant viruses for the improvement of commercial crops. This is mainly because the viruses used to create vectors usually cause significant yield loss and ...

journal_title：Journal of biotechnology

authors： Arazi T,Slutsky SG,Shiboleth YM,Wang Y,Rubinstein M,Barak S,Yang J,Gal-On A

更新日期：2001-04-27 00:00:00

abstract：:Recombinant bacteriophage Qbeta coat protein (CP), which has been proposed as a promising carrier of foreign epitopes via their incorporation either by gene engineering techniques or by chemical coupling, efficiently self-assembles into virus-like particles (VLPs) when expressed in Escherichia coli. Here, we demonstra...

journal_title：Journal of biotechnology

authors： Freivalds J,Dislers A,Ose V,Skrastina D,Cielens I,Pumpens P,Sasnauskas K,Kazaks A

更新日期：2006-05-29 00:00:00

abstract：:The growth of bacteria as structured aggregates termed biofilms leads to their protection from harsh environmental conditions such as physical and chemical stresses, shearing forces, and limited nutrient availability. Because of this highly adapted ability to survive adverse environmental conditions, bacterial biofilm...

journal_title：Journal of biotechnology

authors： Taylor PK,Yeung AT,Hancock RE

更新日期：2014-12-10 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) is the primary causative agent of an economically important swine disease, now known as porcine-associated disease (PCVAD). The only structural protein of viral capsid, Cap has become the major target for development of PCV2 subunit vaccines. The purpose of this study is to express Cap...

journal_title：Journal of biotechnology

authors： Chi JN,Wu CY,Chien MS,Wu PC,Wu CM,Huang C

更新日期：2014-07-10 00:00:00

abstract：:Gene silencing experiments in difficult-to-transfect cells are often hampered by the presence of a background of untransfected cells. We present proof-of-concept data from two different strategies for enrichment of siRNA-transfected cells. In the first approach, a heterologous surface antigen is expressed from a plasm...

journal_title：Journal of biotechnology

authors： Narz F,Hübner S,Magyar S,Bielke W,Weber M,Dennig J

更新日期：2007-06-30 00:00:00

abstract：:Pseudomonas alcaligenes secretes a lipase with a high pH optimum, which has interesting properties for application in detergents. The expression of the lipase is strongly dependent on the presence of lipids in the growth medium such as soybean oil. The promoter of the gene was characterized and found to have resemblan...

journal_title：Journal of biotechnology

authors： Cox M,Gerritse G,Dankmeyer L,Quax WJ

更新日期：2001-03-09 00:00:00

abstract：:The utilisation of urban organic waste as feedstock for polyhydroxyalkanoates (PHA) production is growing since it allows to solve the main concerns about their disposal and simultaneously to recover added-value products. A pilot scale platform has been designed for this purpose. The VFA-rich fermentation liquid comin...

journal_title：Journal of biotechnology

authors： Valentino F,Lorini L,Gottardo M,Pavan P,Majone M

更新日期：2020-11-10 00:00:00

abstract：:The de novo design of polypeptide sequences with a three-dimensional structure necessary for many biological functions is limited by the complex folding process, or 'protein folding problem'. This problem can be bypassed through constructing protein-like molecules with a 'built-in' device for intramolecular folding, t...

journal_title：Journal of biotechnology

authors： Tuchscherer G,Mutter M

更新日期：1995-07-31 00:00:00

abstract：:(S)-2-chlorophenylglycine methyl ester ((S)-1) is a key chiral building block of clopidogrel, which is a widely administered antiaggregatory and antithrombotic drug. Herein, Protease 6SD was covalently immobilized on multi-walled carbon nanotubes (MWCNT), and the as-prepared immobilizate P-6SD@NH2-MWCNT was applied in...

journal_title：Journal of biotechnology

authors： Weng CY,Wang DN,Ban SY,Zhai QY,Hu XY,Cheng F,Wang YJ,Zheng YG

更新日期：2021-01-10 00:00:00

abstract：:Chimeric virus-like particles (VLP) are known as promising tools in the development of safe and effective subunit vaccines. Recently, a technology platform to produce VLP based on the small surface protein (dS) of the duck hepatitis B virus was established. In this study, chimeric VLP were investigated displaying the ...

journal_title：Journal of biotechnology

authors： Wetzel D,Barbian A,Jenzelewski V,Schembecker G,Merz J,Piontek M

更新日期：2019-12-20 00:00:00

abstract：:Norovirus infections cause severe gastroenteritis in millions of people every year. Infection requires the recognition of histo-blood group antigens (HBGAs), but such interactions can be inhibited by human milk oligosaccharides (HMOs), which act as structurally-similar decoys. HMO supplements could help to prevent nor...

journal_title：Journal of biotechnology

authors： Derya SM,Spiegel H,Hanisch FG,Morozov V,Schroten H,Jennewein S,Parschat K

更新日期：2020-07-20 00:00:00

abstract：:This study examined the kinetics and substrate selectivity of a GH11 Bacillus subtilis XynA xylanase (BsX) sensitive to inhibition by TAXI and an engineered variant, which is much less inhibited by TAXI (BsX(mut)). The main purpose of the work was to elucidate any influence of the structural point mutations on the kin...

journal_title：Journal of biotechnology

authors： Rasmussen LE,Sørensen JF,Meyer AS

更新日期：2010-04-15 00:00:00

abstract：:The isolation of ars sequence from the yeast Yarrowia lipolytica has recently been reported (Fournier et al., 1991). Vectors containing ars18 have been used to increase homologous and heterologous protein production. Examples presented are the Yarrowia lipolytica alkaline extracellular protease (AEP), the porcine alph...

journal_title：Journal of biotechnology

authors： Nicaud JM,Fournier P,La Bonnardière C,Chasles M,Gaillardin C

更新日期：1991-07-01 00:00:00

abstract：:This study evaluates the suitability of flow cytometry with the fluorochrome BCECF for measuring the intracellular pH (pHi) of cultured cells, and monitors the changes in pHi in murine hybridoma in batch culture and chick embryo fibroblast in monolayer culture (5th passage). The technique produced highly reproducible,...

journal_title：Journal of biotechnology

authors： Franck P,Petitipain N,Cherlet M,Dardennes M,Maachi F,Schutz B,Poisson L,Nabet P

更新日期：1996-05-15 00:00:00

abstract：:Two different approaches of matrix assisted refolding have been evaluated and compared to conventional refolding by dilution. Bovine alpha-lactalbumin was used for the studies as model protein. It was adsorbed under denaturing conditions on an ion exchange matrix and refolding was completed on the column prior to elut...

journal_title：Journal of biotechnology

authors： Machold C,Schlegl R,Buchinger W,Jungbauer A

更新日期：2005-04-20 00:00:00