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Increased gene expression and inflammatory cell infiltration caused by electroporation are both important for improving the efficacy of DNA vaccines.

Abstract：

:One potential reason for the enhancement of immune responses to DNA vaccines following electroporation is increased gene expression. However, the inflammatory response and accompanying cellular infiltration stimulated by electroporation may also be essential for enhancing immune responses to DNA vaccines. These parameters were investigated in pigs, using different electroporation conditions to induce different levels of gene expression and inflammation. Results indicated that the least effective strategy was conventional intramuscular injection where there was low gene expression and low inflammatory cell infiltration. The most efficacious strategy was plasmid administration immediately followed by electroporation. This latter set of conditions elicited a combination of high gene expression and high cellular infiltration. This indicates that electroporation enhances immune responses to DNA vaccines through increased gene expression and inflammatory cell infiltration.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Babiuk S,Baca-Estrada ME,Foldvari M,Middleton DM,Rabussay D,Widera G,Babiuk LA

doi

10.1016/j.jbiotec.2004.01.015

subject

Has Abstract

pub_date

2004-05-13 00:00:00

pages

1-10

issue

1

eissn

0168-1656

issn

1873-4863

pii

S0168165604000586

journal_volume

110

pub_type

杂志文章

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