Development of an intra-molecularly shuffled efficient chimeric plant promoter from plant infecting Mirabilis mosaic virus promoter sequence.

abstract：:Aqueous two-phase systems allow for the unequal distribution of proteins and other molecules in water-rich solutions containing phase separating polymers or surfactants. One approach to improve the partitioning properties of recombinant proteins is to produce the proteins as fused to certain peptide tags. However, the...

journal_title：Journal of biotechnology

authors： Bandmann N,Van Alstine J,Veide A,Nygren PA

更新日期：2002-01-31 00:00:00

abstract：:Contemporary phytase research is primarily concerned with ameliorating the problem of inadequate digestion of inositol hexakisphosphate (phytate; InsP6) in monogastric farm animal feed, so as to reduce the pollution that results from the high phosphate content of the manure. In the current study we pursue a new, safe ...

journal_title：Journal of biotechnology

authors： Cho J,Choi K,Darden T,Reynolds PR,Petitte JN,Shears SB

更新日期：2006-11-01 00:00:00

abstract：:Cell wall integrity signaling pathway in Saccharomyces cerevisiae is a conserved function for detecting and responding to cell stress conditions but less understood for industrial yeast. We examined gene expression dynamics for a tolerant industrial yeast strain NRRL Y-50049 in response to challenges of furfural and H...

journal_title：Journal of biotechnology

authors： Liu ZL,Wang X,Weber SA

更新日期：2018-06-20 00:00:00

abstract：:The Pol6 mutant of Penicillium occitanis, secreting a large quantity of cellulases, was cultivated in fermentor using a local paper pulp as an inducer substrate. A high titer of extracellular cellulase activity was reached after a fed batch process: 23 IU x ml(-1) filter paper activity, 21 IU x ml(-1) CMCases activity...

journal_title：Journal of biotechnology

authors： Belghith H,Ellouz-Chaabouni S,Gargouri A

更新日期：2001-08-23 00:00:00

abstract：:Harbour sediments are periodically subjected to dredging operations and their management is mainly based on the assessment of the chemical contamination levels, but the potential risks posed by the presence of pathogenic microorganisms have been largely neglected. Here we first developed new molecular protocols based ...

journal_title：Journal of biotechnology

authors： Luna GM,Dell'Anno A,Pietrangeli B,Danovaro R

更新日期：2012-02-20 00:00:00

abstract：:Escherichia coli strain BL21(DE3) was tested as a delivery vector for gene transfer to a murine P388D1 macrophage cell line using a 96-well high-throughput assay. Five recombinant strains of E. coli were compared to identify the effect recombinant listeriolysin O (LLO) and associated gene expression parameters had on ...

journal_title：Journal of biotechnology

authors： Parsa S,Wang Y,Rines K,Pfeifer BA

更新日期：2008-10-10 00:00:00

abstract：:Bifidobacterium catenulatum JCM 1194(T) was isolated from human feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. catenulatum strain. ...

journal_title：Journal of biotechnology

authors： Morita H,Toh H,Oshima K,Nakano A,Yamashita N,Iioka E,Arakawa K,Suda W,Honda K,Hattori M

更新日期：2015-09-20 00:00:00

abstract：:Herbaspirillum seropedicae is a nitrogen-fixing bacterium found in association with economically important gramineae. Regulation of nitrogen fixation involves the transcriptional activator NifA protein. The regulation of NifA protein and its truncated mutant proteins is described and compared with that of other nitrog...

journal_title：Journal of biotechnology

authors： Pedrosa FO,Benelli EM,Yates MG,Wassem R,Monteiro RA,Klassen G,Steffens MB,Souza EM,Chubatsu LS,Rigo LU

更新日期：2001-10-04 00:00:00

abstract：:A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was furthe...

journal_title：Journal of biotechnology

authors： Karmakar B,Vohra RM,Nandanwar H,Sharma P,Gupta KG,Sobti RC

更新日期：2000-07-14 00:00:00

abstract：:Magnetic Fe3O4 nanoparticles were prepared and embedded into the Combi-CLEAs to produce the magnetic Combi-CLEAs in this work. The process for magnetic Combi-CLEAs preparation was optimized, and its properties were investigated. The optimum temperature, thermal stability and optimum pH of magnetic Combi-CLEAs were sim...

journal_title：Journal of biotechnology

authors： Su E,Meng Y,Ning C,Ma X,Deng S

更新日期：2018-04-10 00:00:00

abstract：:Pedobacter cryoconitis PAMC 27485, an aerobic, Gram-negative, facultatively psychrophilic bacterium, was isolated from Antarctic soil. Here we report the complete genome of P. cryoconitis PAMC 27485, which contains a type II CRISPR-Cas system and genes encoding useful enzymes (e.g. proteases). The genome sequence of P...

journal_title：Journal of biotechnology

authors： Lee J,Jung YJ,Lee HK,Hong SG,Kim OS

更新日期：2016-05-20 00:00:00

abstract：:Increasing levels of antibiotic resistance in pathogens, including Staphylococcus aureus, remains a serious problem for public health, leading to the need for better alternative antimicrobial strategies. The antimicrobial proteins produced by Lactobacillus plantarum USM8613 attributed to its anti-staphylococcal activi...

journal_title：Journal of biotechnology

authors： Ong JS,Taylor TD,Wong CB,Khoo BY,Sasidharan S,Choi SB,Ohno H,Liong MT

更新日期：2019-07-20 00:00:00

abstract：:Previously, we showed that the enzymes aspartokinase (AK) and dihydrodipicolinate synthase (DDPS), which are involved in L-lysine biosynthesis in the Gram-negative obligate methylotroph Methylophilus methylotrophus AS1, were inhibited by allosteric effectors, including L-lysine. To elucidate further the regulation of ...

journal_title：Journal of biotechnology

authors： Tsujimoto N,Gunji Y,Ogawa-Miyata Y,Shimaoka M,Yasueda H

更新日期：2006-07-13 00:00:00

abstract：:Oleaginous yeast Trichosporon cutaneum demonstrated excellent lipid accumulation performance and inhibitor tolerance derived from lignocellulose pretreatment. Here we firstly report a 30.45Mb assembly genome of T. cutaneum ACCC 20271 for understanding the microbial lipid biosynthesis and mechanism of inhibitor toleran...

journal_title：Journal of biotechnology

authors： Wang J,Gao Q,Bao J

更新日期：2016-06-20 00:00:00

abstract：:Enzyme engineering by molecular modelling and site-directed mutagenesis can be remarkably efficient. Directed enzyme evolution appears as a more general strategy for the isolation of catalysts as it can be applied to most chemical reactions in aqueous solutions. Selections, as opposed to screening, allow the simultane...

journal_title：Journal of biotechnology

authors： Jestin JL,Kaminski PA

更新日期：2004-09-30 00:00:00

abstract：:A repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a Rhodococcus mutant. The addition of glutamate, thiamine, and MgSO(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance ...

journal_title：Journal of biotechnology

authors： Koike K,Takeuchi K,Mino H,Takaiwa M,Tohoh T,Tadokoro T,Tsutoh K,Ito S

更新日期：2000-06-23 00:00:00

abstract：:Recombinant human granulocyte colony stimulating factor (rhG-CSF) and its PEGylated product "mono-PEG20-GCSF" have already been widely used for treatment of all kinds of neutropenia. However, the high required dosage of mono-PEG20-GCSF made it relatively expensive in clinical use. We postulated that an N-terminal site...

journal_title：Journal of biotechnology

authors： Zhai Y,Zhao Y,Lei J,Su Z,Ma G

更新日期：2009-07-15 00:00:00

abstract：:Nanobiotechnological improvements defined on the utilization of biological materials and principles have enormously partaken to revolutionize physical, chemical, and biological sciences. However, the exploration of plant nanobiotechnology is still in its outset. The search for novel tools to monitor plant biomolecules...

journal_title：Journal of biotechnology

authors： Ambrin G,Kausar H,Ahmad A

更新日期：2020-11-10 00:00:00

abstract：:Polyhydroxyalkanoates (PHA), a family of diverse bio-polyesters, are produced by many bacteria as an energy and carbon storage material. PHA synthesis regulatory protein PhaR was reported to attach on the surface of intracellular PHA granules for convenience of synthesis regulation. PhaR was found to have an amphiphil...

journal_title：Journal of biotechnology

authors： Ma HK,Liu MM,Li SY,Wu Q,Chen JC,Chen GQ

更新日期：2013-06-20 00:00:00

abstract：:The kinetics of microbial growth and the biodegradation of methanol and toluene in (a) biofilters (BFs), and (b) biotrickling filters (BTFs), packed with inert materials, has been studied and analyzed. The specific growth rate, mu, for the treatment of methanol was 0.037h(-1) for a wide range of operating conditions. ...

journal_title：Journal of biotechnology

authors： Avalos Ramirez A,Bénard S,Giroir-Fendler A,Jones JP,Heitz M

更新日期：2008-11-25 00:00:00

abstract：:An NADH-dependent phenylpyruvate reductase (LaPPR) was identified through screening the shotgun library of Lactobacillus sp. CGMCC 9967. It belongs to D-3-phosphoglycerate dehydrogenase (PGDH) subfamily of 2-hydroxy acid dehydrogenase superfamily. LaPPR was stable at pH 6.5 and 30 °C, with a half-life of 152 h. LaPPR ...

journal_title：Journal of biotechnology

authors： Xu GC,Zhang LL,Ni Y

更新日期：2016-03-20 00:00:00

abstract：:Many representatives of the order Actinomycetales are prolific producers of thousands of biologically active secondary metabolites. Actinomycetes from terrestrial sources have been studied and screened since the 1950s, yielding many important anti-infective and anti-cancer drugs. However, frequent re-discovery of the ...

journal_title：Journal of biotechnology

authors： Zotchev SB

更新日期：2012-04-30 00:00:00

abstract：:Two electronic tongues based on different measurement techniques were applied to the discrimination of four molds and one yeast. Chosen microorganisms were different species of Aspergillus and yeast specie Zygosaccharomyces bailii, which are known as food contaminants. The electronic tongue developed in Linköping Univ...

journal_title：Journal of biotechnology

authors： Söderström C,Rudnitskaya A,Legin A,Krantz-Rülcker C

更新日期：2005-09-29 00:00:00

abstract：:The current validated endotoxin detection methods, in spite of being highly sensitive, present several drawbacks in terms of reproducibility, handling and cost. Therefore novel approaches are being carried out in the scientific community to overcome these difficulties. Remarkable efforts are focused on the development...

journal_title：Journal of biotechnology

authors： Mujika M,Zuzuarregui A,Sánchez-Gómez S,Martínez de Tejada G,Arana S,Pérez-Lorenzo E

更新日期：2014-09-30 00:00:00

abstract：:It has been well recognized that many key aspects of cell cycle regulation are encoded into the size distributions of growing budding yeast populations due to the tight coupling between cell growth and cell division present in this organism. Several attempts have been made to model the cell size distribution of growin...

journal_title：Journal of biotechnology

authors： Hatzis C,Porro D

更新日期：2006-07-13 00:00:00

abstract：:Plant virus vectors provide an attractive biotechnological tool for the transient expression of foreign genes in whole plants. As yet there has been no use of recombinant viruses for the improvement of commercial crops. This is mainly because the viruses used to create vectors usually cause significant yield loss and ...

journal_title：Journal of biotechnology

authors： Arazi T,Slutsky SG,Shiboleth YM,Wang Y,Rubinstein M,Barak S,Yang J,Gal-On A

更新日期：2001-04-27 00:00:00

abstract：:Anaerobic co-digestion using protein-rich and lipid-rich co-substrates is limited by the accumulation of ammonia and long chain fatty acids (LCFAs), which are important inhibitors of the anaerobic microorganisms. This work aimed to study the microbial community dynamics during gradual and abrupt increase in ammonium a...

journal_title：Journal of biotechnology

authors： Regueiro L,Carballa M,Lema JM

更新日期：2016-02-20 00:00:00

abstract：:A system has been developed for the release of heterologous proteins from Saccharomyces cerevisiae, based on the use of thermosensitive osmotic-remedial mutants, deficient in cell integrity, that lyse at the non-permissive temperature, thus releasing the bulk of intracellular proteins and leaving behind cell ghosts an...

journal_title：Journal of biotechnology

authors： Alvarez P,Sampedro M,Molina M,Nombela C

更新日期：1994-11-30 00:00:00

abstract：:Two affinity columns comprising immobilized concanavalin A (Con A), Con A-Sepharose and Con A-XP3507, were evaluated for their purifying ability for the glycoprotein allergen Ag7 from a partially purified extract of mugwort pollen. The most pronounced difference between the two columns was the nature of their nonspeci...

journal_title：Journal of biotechnology

authors： Nilsen BM,Smestad Paulsen B,Clonis Y,Mellbye KS

更新日期：1990-11-01 00:00:00

abstract：:Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 microm) of macroporous poly(dimethylacrylamide) makes it possible to couple l...

journal_title：Journal of biotechnology

authors： Noppe W,Plieva FM,Vanhoorelbeke K,Deckmyn H,Tuncel M,Tuncel A,Galaev IY,Mattiasson B

更新日期：2007-09-15 00:00:00