Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds.

Abstract:

:Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 microm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 microm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1-10 microm in size) or even whole blood containing blood cells (up to 20 microm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Noppe W,Plieva FM,Vanhoorelbeke K,Deckmyn H,Tuncel M,Tuncel A,Galaev IY,Mattiasson B

doi

10.1016/j.jbiotec.2007.06.021

subject

Has Abstract

pub_date

2007-09-15 00:00:00

pages

293-9

issue

3

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(07)00954-6

journal_volume

131

pub_type

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