Complete genome sequence of Bifidobacterium catenulatum JCM 1194(T) isolated from human feces.

abstract：:Real-Time PCR has been applied to quantify extraradical soil mycelium of the edible ectomycorrhizal fungus Lactarius deliciosus in an interspecific competition experiment under greenhouse conditions. Couples of Pinus pinea seedlings inoculated with either L. deliciosus, Rhizopogon roseolus, or non-inoculated (control)...

journal_title：Journal of biotechnology

authors： Parladé J,Hortal S,Pera J,Galipienso L

更新日期：2007-01-30 00:00:00

abstract：:We previously showed that creation of a double strand DNA break (DSB) by expressing I-SceI in an engineered Trichoderma reesei (Hypocrea jecorina) strain containing a I-SceI recognition site improved transformation and homologous integration efficiencies. In this study, we further improved homologous integration frequ...

journal_title：Journal of biotechnology

authors： Ouedraogo JP,Arentshorst M,Nikolaev I,Barends S,Ram AF

更新日期：2016-03-20 00:00:00

abstract：:Gene farming is one of the most promising areas in modern biotechnology. To assay the potential usefulness of transgenic rabbits as bioreactors, one call embryos were microinjected with a chimeric gene comprising 5' sequences from mouse whey acidic protein gene (mWAP) linked to the human growth hormone (hGH) gene. Tra...

journal_title：Journal of biotechnology

authors： Limonta JM,Castro FO,Martínez R,Puentes P,Ramos B,Aguilar A,Lleonart RL,de la Fuente J

更新日期：1995-05-15 00:00:00

abstract：:Inoculation of naked DNA has been applied for the development of prophylactic and therapeutic vaccines against different viral infections. To study the humoral immune response induced by DNA vaccination we cloned the entire nucleocapsid protein-encoding sequence of the Puumala hantavirus strain Vranica/Hällnäs into th...

journal_title：Journal of biotechnology

authors： Koletzki D,Schirmbeck R,Lundkvist A,Meisel H,Krüger DH,Ulrich R

更新日期：2001-11-17 00:00:00

abstract：:A highly active and stable cross-linked enzyme aggregates (CLEAs) of epoxide hydrolases (EHs) from Mung bean, which plays a crucial role in synthesis of valuable enantiopure diols, were successfully prepared and characterized. Under the optimum preparation conditions, the activity recovery of CLEAs recorded 92%. The C...

journal_title：Journal of biotechnology

authors： Yu CY,Li XF,Lou WY,Zong MH

更新日期：2013-06-20 00:00:00

abstract：:The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacter...

journal_title：Journal of biotechnology

authors： Tan IKP,Foong CP,Tan HT,Lim H,Zain NA,Tan YC,Hoh CC,Sudesh K

更新日期：2020-04-10 00:00:00

abstract：:Epigallocatechin-3-O-gallate (EGCg) and related polyphenolic compounds found in tea are known to have antioxidative activities. However, they also have pro-oxidative activities such as generation of hydrogen peroxide. In this report, we investigated the effect on cells and showed the potential usage of EGCg in cell pr...

journal_title：Journal of biotechnology

authors： Matsumura K,Kim JY,Tsutsumi S,Hyon SH

更新日期：2007-01-20 00:00:00

abstract：:Super-resolution fluorescence imaging methods based on reversible photoswitching of fluorophores with subsequent localization currently develop to promising tools for cellular imaging. Since most of these methods rely on the transfer of the majority of fluorophores to a non-fluorescent dark state and precise localizat...

journal_title：Journal of biotechnology

authors： van de Linde S,Wolter S,Heilemann M,Sauer M

更新日期：2010-09-15 00:00:00

abstract：:Recombinant human granulocyte colony stimulating factor (rhG-CSF) and its PEGylated product "mono-PEG20-GCSF" have already been widely used for treatment of all kinds of neutropenia. However, the high required dosage of mono-PEG20-GCSF made it relatively expensive in clinical use. We postulated that an N-terminal site...

journal_title：Journal of biotechnology

authors： Zhai Y,Zhao Y,Lei J,Su Z,Ma G

更新日期：2009-07-15 00:00:00

abstract：:Increasing levels of antibiotic resistance in pathogens, including Staphylococcus aureus, remains a serious problem for public health, leading to the need for better alternative antimicrobial strategies. The antimicrobial proteins produced by Lactobacillus plantarum USM8613 attributed to its anti-staphylococcal activi...

journal_title：Journal of biotechnology

authors： Ong JS,Taylor TD,Wong CB,Khoo BY,Sasidharan S,Choi SB,Ohno H,Liong MT

更新日期：2019-07-20 00:00:00

abstract：:The genus Enterococcus occurs as native microbiota of fermented products due to its broad environmental distribution and its resistance to salt concentrations. Enterococcus faecalis F, a non-pathogenic strain isolated from a ripened cheese, has demonstrated useful enzymatic capabilities, a probiotic behavior and antib...

journal_title：Journal of biotechnology

authors： Serrano-Maldonado CE,Quirasco M

更新日期：2018-10-10 00:00:00

abstract：:This paper introduces a novel approach to enhance the strains' biodegradation of 4-chloronitrobenzene by utilizing the synergistic effect of the organic reductant mannitol and the substrate beef extraction. Our results demonstrate that 4-chloronitrobenzene could not be an available nitrogen source to support target st...

journal_title：Journal of biotechnology

authors： Li T,Zhang TC,He L

更新日期：2017-12-20 00:00:00

abstract：:Based on the importance of heat shock proteins (HSPs) in diseases such as cancer, Alzheimer's disease or malaria, inhibitors of these chaperons are needed. Today's state-of-the-art techniques to identify HSP inhibitors are performed in microplate format, requiring large amounts of proteins and potential inhibitors. In...

journal_title：Journal of biotechnology

authors： Schax E,Walter JG,Märzhäuser H,Stahl F,Scheper T,Agard DA,Eichner S,Kirschning A,Zeilinger C

更新日期：2014-06-20 00:00:00

abstract：:The stability, copy number, and gene expression of the pBR322 plasmid containing the par-locus under prolonged cultivation were studied. In the initial stage of the experiment it was observed that the par-locus had a stabilization effect on plasmid maintenance. This observation was consistent with previously reported ...

journal_title：Journal of biotechnology

authors： Weber AE,Yu P,San KY

更新日期：1991-04-01 00:00:00

abstract：:The potential of electric current-mediated delivery technology to enhance DNA delivery and DNA vaccine potency was evaluated. Higher levels of reporter gene expression were observed in muscle cells of mice inoculated with luciferase or beta-galactosidase DNA followed by the application of electrical current, compared ...

journal_title：Journal of biotechnology

authors： Selby M,Goldbeck C,Pertile T,Walsh R,Ulmer J

更新日期：2000-09-29 00:00:00

abstract：:Enterobacter cloacae is a facultative anaerobic bacterium to be an important cause of nosocomial infection. However, the isolated E. cloacae GGT036 showed higher furfural-tolerant cellular growth, compared to industrial relevant strains such as Escherichia coli and Corynebacterium glutamicum. Here, we report the compl...

journal_title：Journal of biotechnology

authors： Gong G,Um Y,Park TH,Woo HM

更新日期：2015-01-10 00:00:00

abstract：:The use of the fungus Aspergillus niger for the bioleaching of heavy metals from spent catalyst was investigated, with fluid catalytic cracking (FCC) catalyst as a model. Bioleaching was examined in batch cultures with the spent catalysts at various pulp densities (1-12%). Chemical leaching was also performed using mi...

journal_title：Journal of biotechnology

authors： Aung KM,Ting YP

更新日期：2005-03-16 00:00:00

abstract：:An assay was developed to measure the hydrophobic interactions of commonly used mammalian cell lines grown in culture. The assay depends on the loss of cells from an aqueous suspension following vortexing with a hydrophobic oil phase. This allowed the determination of a hydrophobicity index, which was significantly hi...

journal_title：Journal of biotechnology

authors： Ghebeh H,Gillis J,Butler M

更新日期：2002-04-25 00:00:00

abstract：:A control strategy to avoid development of synchronous growth in carbohydrate limited Saccharomyces cerevisiae cultivations is proposed and experimentally investigated. The basic idea is to control the metabolic flux through the pathways by manipulating the substrate feed rate to keep the ethanol concentration at a lo...

journal_title：Journal of biotechnology

authors： Møller H,Jørgensen SB

更新日期：1997-02-28 00:00:00

abstract：:New emerging technologies delivering benefits in terms of process robustness and economy are an inevitable prerequisite for monoclonal antibody purification processes intensification. Caprylic acid was proven as an effective precipitating agent enabling efficient precipitaton of product- and process-related impurities...

journal_title：Journal of biotechnology

authors： Trapp A,Faude A,Hörold N,Schubert S,Faust S,Grob T,Schmidt S

更新日期：2018-08-10 00:00:00

abstract：:Indigo is an insoluble blue dye, which generates serious pollution in its production process. Increasing focus has come to the biosynthesis of indigo that are more environment-preserved and high-efficient. Hence, this study was designed to explore the specific role of various deep eutectic solvents (DESs) on cytochrom...

journal_title：Journal of biotechnology

authors： Sun H,Xin R,Qu D,Yao F

更新日期：2020-11-10 00:00:00

abstract：:Kinetic models have a great potential for metabolic engineering applications. They can be used for testing which genetic and regulatory modifications can increase the production of metabolites of interest, while simultaneously monitoring other key functions of the host organism. This work presents a methodology for in...

journal_title：Journal of biotechnology

authors： Villaverde AF,Bongard S,Mauch K,Balsa-Canto E,Banga JR

更新日期：2016-03-20 00:00:00

abstract：:Mevalonate (MVA) is a key compound of living organisms including bacteria, plants, and humans. MVA and mevalonolactone (MVL), a lactonized form of MVA, are important for pharmaceutical, cosmeceutical, and biotechnological applications. Although (R, S)-MVA with 50% enantiomeric purity is mainly produced by chemical syn...

journal_title：Journal of biotechnology

authors： Kim JW,Yoon HC,Kwon SJ,Lee BY,Lee PC

更新日期：2017-10-10 00:00:00

abstract：:Flavonoids have notable biological activities and have been widely used in the medicinal and chemical industries. However, single-copy integration of heterologous pathway genes limits the production of flavonoids. In this work, we designed and constructed single-step integration of multiple flavonoid (2S)-naringenin b...

journal_title：Journal of biotechnology

authors： Li H,Gao S,Zhang S,Zeng W,Zhou J

更新日期：2021-01-10 00:00:00

abstract：:A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was furthe...

journal_title：Journal of biotechnology

authors： Karmakar B,Vohra RM,Nandanwar H,Sharma P,Gupta KG,Sobti RC

更新日期：2000-07-14 00:00:00

abstract：:Escherichia coli strain BL21(DE3) was tested as a delivery vector for gene transfer to a murine P388D1 macrophage cell line using a 96-well high-throughput assay. Five recombinant strains of E. coli were compared to identify the effect recombinant listeriolysin O (LLO) and associated gene expression parameters had on ...

journal_title：Journal of biotechnology

authors： Parsa S,Wang Y,Rines K,Pfeifer BA

更新日期：2008-10-10 00:00:00

abstract：:The production of the blue pigment indigoidine has been achieved in the entomopathogenic bacterium Photorhabdus luminescens by a promoter exchange and in Escherichia coli following heterologous expression of the biosynthesis gene indC. Moreover, genes involved in the regulation of this previously "silent" biosynthesis...

journal_title：Journal of biotechnology

authors： Brachmann AO,Kirchner F,Kegler C,Kinski SC,Schmitt I,Bode HB

更新日期：2012-01-01 00:00:00

abstract：:The expression of recombinant yeast pyruvate carboxylase (PYC) in animal cell lines was shown in previous studies to reduce significantly the formation of waste metabolites, although it has translated into mixed results in terms of improved cellular growth and productivity. In this work, we demonstrate that the unique...

journal_title：Journal of biotechnology

authors： Vallée C,Durocher Y,Henry O

更新日期：2014-01-01 00:00:00

abstract：:In this paper, we report the chromium(VI) reduction by filtrates of Acidithiobacillus and Thiobacillus cultures. Chromium(VI) reduction by filtrates of A. ferrooxidans cultures under acidic conditions was higher than that observed for A. thiooxidans. However, at pH close to 7, chromium(VI) reduction by filtrates of T....

journal_title：Journal of biotechnology

authors： Allegretti P,Furlong J,Donati E

更新日期：2006-03-09 00:00:00

abstract：:A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) is a novel family of extracellular proteases supposedly involved in inflammation, angiogenesis, development and coagulation. To overexpress the active ADAMTS proteins, we designed a chimeric molecule composed of a catalytic domain of ADAMTS-1...

journal_title：Journal of biotechnology

authors： Sugiura T

更新日期：2003-02-13 00:00:00