Abstract：
:Magnetic Fe3O4 nanoparticles were prepared and embedded into the Combi-CLEAs to produce the magnetic Combi-CLEAs in this work. The process for magnetic Combi-CLEAs preparation was optimized, and its properties were investigated. The optimum temperature, thermal stability and optimum pH of magnetic Combi-CLEAs were similar to those of Combi-CLEAs. The catalytic performance of magnetic Combi-CLEAs was tested with the biosynthesis of (S)-ethyl 4-chloro-3-hydroxybutyrate ((S)-CHBE). Magnetic Combi-CLEAs could tolerate higher substrate concentration in the biphasic system. The catalytic efficiency and long-term operational stability of magnetic Combi-CLEAs were obviously superior to those of Combi-CLEAs in both aqueous and biphasic systems. Embedding of magnetic Fe3O4 nanoparticles endowing rigidity contributed to these improvements. Furthermore, the preparation of magnetic Combi-CLEAs was easy, and its recovery during multiple batches of reactions could be fulfilled by magnetic field. Aforementioned advantages make the magnetic Combi-CLEAs hold obvious potential for industrial application.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Su E,Meng Y,Ning C,Ma X,Deng Sdoi
10.1016/j.jbiotec.2018.02.007subject
Has Abstractpub_date
2018-04-10 00:00:00pages
1-7eissn
0168-1656issn
1873-4863pii
S0168-1656(18)30051-8journal_volume
271pub_type
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