Simultaneous saccharification and co-fermentation of glucose and xylose in steam-pretreated corn stover at high fiber content with Saccharomyces cerevisiae TMB3400.

abstract：:The use of high capacity micron-sized non-porous magnetic metal chelator adsorbents for the direct recovery of a recombinant metal-binding protein from crude liquors is described. Selectivity and interaction strength of magnetic chelator particles were assessed using a set of native proteins with known behaviour towar...

journal_title：Journal of biotechnology

authors： O'Brien SM,Sloane RP,Thomas OR,Dunnill P

更新日期：1997-04-04 00:00:00

abstract：:A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were ...

journal_title：Journal of biotechnology

authors： Dinu D,Nechifor MT,Stoian G,Costache M,Dinischiotu A

更新日期：2007-08-31 00:00:00

abstract：:Biodegradable scaffolds are of great value in tissue engineering. We have developed a method for fabricating patient-specific vascular scaffolds from a biocompatible and biodegradable polymer, poly(L-lactide-co-epsilon-caprolactone). This method's usefulness is due to flexibility in the choice of materials and vascula...

journal_title：Journal of biotechnology

authors： Uchida T,Ikeda S,Oura H,Tada M,Nakano T,Fukuda T,Matsuda T,Negoro M,Arai F

更新日期：2008-01-20 00:00:00

abstract：:Oleaginous yeast Trichosporon cutaneum demonstrated excellent lipid accumulation performance and inhibitor tolerance derived from lignocellulose pretreatment. Here we firstly report a 30.45Mb assembly genome of T. cutaneum ACCC 20271 for understanding the microbial lipid biosynthesis and mechanism of inhibitor toleran...

journal_title：Journal of biotechnology

authors： Wang J,Gao Q,Bao J

更新日期：2016-06-20 00:00:00

abstract：:A Gram-negative, rod-shaped, non-motile, gamma and UV radiation resistant bacterium Hymenobacter radioresistens DG25B was isolated from a soil sample collected in South Korea. The complete genome sequence of H. radioresistens DG25B consists of one circular chromosome (3,874,646 bp). The bacterium was isolated from gam...

journal_title：Journal of biotechnology

authors： Kim MK,Joo ES,Lee SY,Lee DS,Srinivasan S,Jung HY

更新日期：2016-01-10 00:00:00

abstract：:A macrokinetic model for Pichia pastoris expressing recombinant human serum albumin is proposed. The model describes the balances of some key metabolites, ATP and NADH, during glycerol and methanol metabolism. In the glycerol growth phase, the metabolic pathways mainly include phosphorylation, glycolysis, tricarboxyli...

journal_title：Journal of biotechnology

authors： Ren HT,Yuan JQ,Bellgardt KH

更新日期：2003-12-05 00:00:00

abstract：:The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine ...

journal_title：Journal of biotechnology

authors： Capiaumont J,Legrand C,Carbonell D,Dousset B,Belleville F,Nabet P

更新日期：1995-02-21 00:00:00

abstract：:NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich d...

journal_title：Journal of biotechnology

authors： Noda T,Kawamura R,Funabashi H,Mie M,Kobatake E

更新日期：2006-11-01 00:00:00

abstract：:The catalytic efficiency and pH optimum of Trichoderma reesei endo-beta-1,4-glucanase II were improved by protein engineering. We subjected residue 342 to saturation mutagenesis, and further changed the enzyme by random mutagenesis and two rounds of DNA shuffling. Enzyme variants were purified and characterized. Varia...

journal_title：Journal of biotechnology

authors： Qin Y,Wei X,Song X,Qu Y

更新日期：2008-06-01 00:00:00

abstract：:Recombinant human granulocyte colony stimulating factor (rhG-CSF) and its PEGylated product "mono-PEG20-GCSF" have already been widely used for treatment of all kinds of neutropenia. However, the high required dosage of mono-PEG20-GCSF made it relatively expensive in clinical use. We postulated that an N-terminal site...

journal_title：Journal of biotechnology

authors： Zhai Y,Zhao Y,Lei J,Su Z,Ma G

更新日期：2009-07-15 00:00:00

abstract：:Human prolactin (hPRL) is a 199 aminoacid protein hormone with a wide spectrum of biological activities which is best known for its stimulation of lactation and development of mammary gland. This protein contains only one potential asparagine-linked glycosylation site, which is partially (10-30%) occupied when the pro...

journal_title：Journal of biotechnology

authors： Heller SR,Rodrigues Goulart H,Arthuso FS,Oliveira TL,Bartolini P,Soares CR

更新日期：2010-02-15 00:00:00

abstract：:The enzymatic bioconversion of chitin is of considerable interest for the natural production of bioactive compounds such as chitooligosaccharides and N-acetyl-d-glucosamine (GlcNAc). Key enzymes are involved in the natural processing of chitin, hydrolyzing this abundant biopolymer to yield chitooligosaccharides with s...

journal_title：Journal of biotechnology

authors： Nguyen-Thi N,Doucet N

更新日期：2016-02-20 00:00:00

abstract：:Dilute-acid lignocellulosic hydrolyzate was successfully fermented to ethanol by encapsulated Saccharomyces cerevisiae at dilution rates up to 0.5h(-1). The hydrolyzate was so toxic that freely suspended yeast cells could ferment it continuously just up to dilution rate 0.1h(-1), where the cells lost 75% of their viab...

journal_title：Journal of biotechnology

authors： Talebnia F,Taherzadeh MJ

更新日期：2006-09-18 00:00:00

abstract：:To study the influence of N-linked carbohydrate moiety on the catalytic and biochemical properties of glycosylated enzyme, a recombinant β-d-glucuronidase (PGUS-P) from Penicillium purpurogenum as a model glycoprotein, was deglycosylated with peptide-N-glycosidase F (PNGase-F) under native conditions. The enzymatic de...

journal_title：Journal of biotechnology

authors： Zou S,Xie L,Liu Y,Kaleem I,Zhang G,Li C

更新日期：2012-02-10 00:00:00

abstract：:Beta-glucosidase genes of fungal origins were isolated and heterologously expressed in Saccharomyces cerevisiae to enable growth on the disaccharide, cellobiose. To promote secretion of the beta-glucosidases, the genes were fused to the secretion signal of the Trichoderma reesei xyn2 gene and constitutively expressed ...

journal_title：Journal of biotechnology

authors： van Rooyen R,Hahn-Hägerdal B,La Grange DC,van Zyl WH

更新日期：2005-11-21 00:00:00

abstract：:An enzymatically-active fungal cellobiohydrolase I (CBH I) was first synthesized in a coupled reticulocyte lysate system lacking of glycosylation modification by the template DNA(Cbh1) in the presence of T7 RNA polymerase. The synthesized CBH I had the expected size (57 kDa) and catalyzed the substrate of p-nitropheny...

journal_title：Journal of biotechnology

authors： Wang J,Gao P

更新日期：2000-08-25 00:00:00

abstract：:A newly developed flat panel airlift photobioreactor with a defined circulation path was tested for microalgal culture. The bioreactor exposed the cells to intermittent light to improve the efficiency of light utilization through the flashing-light effect. During batch cultures in the new photobioreactor, the biomass ...

journal_title：Journal of biotechnology

authors： Degen J,Uebele A,Retze A,Schmid-Staiger U,Trösch W

更新日期：2001-12-28 00:00:00

abstract：:As the understanding of variation is the key to a good process and product quality one should pay attention to dynamics on the single-cell level. The basic idea of this approach was to qualify and quantify variations on the single-cell level during bioreactor cultivations by monitoring the expression of an eGFP tagged...

journal_title：Journal of biotechnology

authors： Broger T,Odermatt RP,Huber P,Sonnleitner B

更新日期：2011-07-20 00:00:00

abstract：:Contemporary phytase research is primarily concerned with ameliorating the problem of inadequate digestion of inositol hexakisphosphate (phytate; InsP6) in monogastric farm animal feed, so as to reduce the pollution that results from the high phosphate content of the manure. In the current study we pursue a new, safe ...

journal_title：Journal of biotechnology

authors： Cho J,Choi K,Darden T,Reynolds PR,Petitte JN,Shears SB

更新日期：2006-11-01 00:00:00

abstract：:We investigated the growth kinetics and transcriptional responses of Corynebacterium glutamicum in environments with low (pCO2<40 mbar) and high (pCO2 ≥ 300 mbar) CO2/HCO3(-) levels compared to standard conditions. When cultivated at high CO2/HCO3(-)-levels, C. glutamicum showed increased (63%) biomass to substrate yi...

journal_title：Journal of biotechnology

authors： Blombach B,Buchholz J,Busche T,Kalinowski J,Takors R

更新日期：2013-12-01 00:00:00

abstract：:Stem cell sensors have emerged as a promising technique to electrochemically monitor the functional status and viability of stem cells. However, efficient electrochemical analysis techniques are required for the development of effective electrochemical stem cell sensors. In the current study, we report a newly develop...

journal_title：Journal of biotechnology

authors： Yea CH,An JH,Kim J,Choi JW

更新日期：2013-06-20 00:00:00

abstract：:The elemental composition of microorganisms varies widely with respect to the N, S, P and ash content as well as many trace elements depending on microorganism and growth conditions. As a consequence, the mass of 1 C-mol of biomass can differ considerably from one microorganism to another. Experimental results show th...

journal_title：Journal of biotechnology

authors： Duboc P,Schill N,Menoud L,van Gulik W,von Stockar U

更新日期：1995-12-01 00:00:00

abstract：:A novel method for immobilizing large DNA fragments on a solid surface was developed. A mixed self-assembled monolayer of thiolated single-stranded DNA with inert alkanethiol was generated on a gold (Au) surface through the Au-S reaction. Surface-tethered DNA generated by this method was compatible with various geneti...

journal_title：Journal of biotechnology

authors： Kim JH,Hong JA,Yoon M,Yoon MY,Jeong HS,Hwang HJ

更新日期：2002-07-03 00:00:00

abstract：:Porcine muscle adenylate kinase (ADK) was overproduced in Escherichia coli using the expression plasmid with double A-T-G codon at the translational starting site and the Shine-Dalgarno (SD) sequence 10 bp apart from the first A-T-G. We used the expression vectors pKK223-3 and pMK2. pMK2 is about 10-20 times larger in...

journal_title：Journal of biotechnology

authors： Hibino T,Misawa S,Wakiyama M,Maeda S,Yazaki K,Kumagai I,Ooi T,Miura K

更新日期：1994-02-14 00:00:00

abstract：:The present study aims to valorize chestnut burrs, an important lignocellulosic waste, through a biorefinery concept. A solid residue rich in glucan (41.36 ± 0.59 %) and lignin (39.06 ± 0.01 %) obtained from a previous process of pre-hydrolysis was subjected to four treatments with water or NaOH to enhance enzymatic h...

journal_title：Journal of biotechnology

authors： Costa-Trigo I,Paz A,Otero-Penedo P,Outeiriño D,Pérez Guerra N,Domínguez JM

更新日期：2021-01-18 00:00:00

abstract：:Cancer immunotherapy is a promising intervention to fight against this global health problem. In particular targeting immune checkpoints, such as cytotoxic T-lymphocyte associated antigen-4 (CTLA-4) and programmed-death protein 1 (PD-1), by specific monoclonal antibodies is a current treatment for many malignances. A ...

journal_title：Journal of biotechnology

authors： Yiemchavee S,Wong-Arce A,Romero-Maldonado A,Shanmugaraj B,Monsivais-Urenda A,Phoolcharoen W,Rosales-Mendoza S

更新日期：2021-01-21 00:00:00

abstract：:The genome of the biotechnologically important solventogenic Clostridium saccharobutylicum NCP 262 contains two operons coding for genes of presumed type I RM systems belonging to the families A and C. They represent a limiting factor for the development of transformation and conjugation protocols. We established an e...

journal_title：Journal of biotechnology

authors： Lesiak JM,Liebl W,Ehrenreich A

更新日期：2014-10-20 00:00:00

abstract：:While chromatin immunoprecipitation has become a widely-used method in the field of transcription regulation studies, serious limitations connected to the complexity and relatively little standardization of the method serve as obstacles for its use in clinical research. In this paper we introduce a method for developi...

journal_title：Journal of biotechnology

authors： Keresztessy Z,Erdos E,Ozgyin L,Kádas J,Horváth J,Zahuczky G,Balint BL

更新日期：2019-07-20 00:00:00

abstract：:The synthesis of 4-methylumbelliferyl 3-beta-O-cellobiosyl-beta-D-glucopyranoside (3a) and its use as specific substrate to monitor enzyme activity of 1,3-1,4-beta-D-glucan 4-glucanohydrolases are described. The chromophoric substrate 3a is prepared by a chemoenzymatic approach starting from barley grain, whose beta-D...

journal_title：Journal of biotechnology

authors： Malet C,Vallés J,Bou J,Planas A

更新日期：1996-07-31 00:00:00

abstract：:To study the metaproteome of a biogas-producing microbial community, fermentation samples were taken from an agricultural biogas plant for microbial cell and protein extraction and corresponding metagenome analyses. Based on metagenome sequence data, taxonomic community profiling was performed to elucidate the composi...

journal_title：Journal of biotechnology

authors： Ortseifen V,Stolze Y,Maus I,Sczyrba A,Bremges A,Albaum SP,Jaenicke S,Fracowiak J,Pühler A,Schlüter A

更新日期：2016-08-10 00:00:00