Transduction of NeuroD2 protein induced neural cell differentiation.


:NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich domains, one is nuclear localization signal (NLS) and the other is basic region of basic helix-loop-helix (basic). We constructed some mutants of NeuroD2, ND2(Delta100-115) (lack of NLS), ND2(Delta123-134) (lack of basic) and ND2(Delta100-134) (lack of both NLS and basic) for transduction experiments. Using these proteins, we have shown that NLS region of NeuroD2 plays a role of protein transduction. Continuous addition of NeuroD2 protein resulted in N1E-115 cells adopting neural morphology after 4 days and Tau mRNA expression was increased. These results suggest that neural differentiation can be induced by direct addition of NeuroD2 protein.


J Biotechnol


Journal of biotechnology


Noda T,Kawamura R,Funabashi H,Mie M,Kobatake E




Has Abstract


2006-11-01 00:00:00














  • Efficient enzymatic synthesis of ampicillin by mutant Alcaligenes faecalis penicillin G acylase.

    abstract::Semi-synthetic β-lactam antibiotics (SSBAs) are one of the most important antibiotic families in the world market. Their enzymatic synthesis can be catalyzed by penicillin G acylases (PGAs). In this study, to improve enzymatic synthesis of ampicillin, site-saturating mutagenesis was performed on three conserved amino ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Deng S,Su E,Ma X,Yang S,Wei D

    更新日期:2015-04-10 00:00:00

  • Histamine dehydrogenase from Rhizobium sp.: gene cloning, expression in Escherichia coli, characterization and application to histamine determination.

    abstract::The gene encoding histamine dehydrogenase in Rhizobium sp. 4--9 has been cloned and overexpressed in Escherichia coli. The coding region of the gene was 2,079 bp and encoded a protein of 693 amino acids with a calculated molecular mass of 76,732 Da. This histamine dehydrogenase was related to histamine dehydrogenase f...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Bakke M,Sato T,Ichikawa K,Nishimura I

    更新日期:2005-09-29 00:00:00

  • Simultaneous biosynthesis of (+)-geodin by a lovastatin-producing fungus Aspergillus terreus.

    abstract::The simultaneous biosynthesis of lovastatin (mevinolinic acid) and (+)-geodin by Aspergillus terreus ATCC 20542 with regard to the medium composition, i.e. the concentrations of carbon and nitrogen source, was described in this paper. A. terreus is a lovastatin producer but the formation of lovastatin was accompanied ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Bizukojc M,Ledakowicz S

    更新日期:2007-12-01 00:00:00

  • Replacement of an amino acid residue of cyclodextrin glucanotransferase of Bacillus ohbensis doubles the production of gamma-cyclodextrin.

    abstract::Cyclodextrin glucanotransferase (CGTase; EC produces cyclodextrin (CD) from starch through an intramolecular transglucosylation reaction. To obtain a better understanding of the amylolytic and cyclization mechanisms of CGTase, and furthermore to improve the production of gamma-CD, mutant CGTases were constru...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Sin KA,Nakamura A,Masaki H,Matsuura Y,Uozumi T

    更新日期:1994-02-28 00:00:00

  • Single-stranded DNA aptamers that bind differentiated but not parental cells: subtractive systematic evolution of ligands by exponential enrichment.

    abstract::In this paper, single-stranded (ss)DNA aptamers with capability to distinguish differentiated PC12 cells from normal PC12 cells were selected by subtractive systematic evolution of ligands by exponential enrichment (SELEX) method. Before each round of selection, randomized ssDNAs were incubated with regular PC12 cells...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Wang C,Zhang M,Yang G,Zhang D,Ding H,Wang H,Fan M,Shen B,Shao N

    更新日期:2003-04-10 00:00:00

  • Biostoning of denims by Penicillium occitanis (Pol6) cellulases.

    abstract::The Pol6 mutant of Penicillium occitanis, secreting a large quantity of cellulases, was cultivated in fermentor using a local paper pulp as an inducer substrate. A high titer of extracellular cellulase activity was reached after a fed batch process: 23 IU x ml(-1) filter paper activity, 21 IU x ml(-1) CMCases activity...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Belghith H,Ellouz-Chaabouni S,Gargouri A

    更新日期:2001-08-23 00:00:00

  • Expression of recombinant cytoplasmic yeast pyruvate carboxylase for the improvement of the production of human erythropoietin by recombinant BHK-21 cells.

    abstract::Recently, a recombinant yeast pyruvate carboxylase expressed in the cytoplasm of BHK-21 cells was shown to reconstitute the missing link between glycolysis and TCA, thus increasing the flux of glucose into the TCA and resulting in a higher intracellular ATP content. Now, these metabolically engineered cells have been ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Irani N,Beccaria AJ,Wagner R

    更新日期:2002-02-28 00:00:00

  • Direct oligonucleotide synthesis onto super-paramagnetic beads.

    abstract::Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedure of direct oligon...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Jensen MA,Akhras MS,Fukushima M,Pourmand N,Davis RW

    更新日期:2013-09-20 00:00:00

  • Bovine beta-lactoglobulin receptors on transformed mammalian cells (hybridomas MARK-3): characterization by flow cytometry.

    abstract::Flow cytometry was used to demonstrate the presence of beta-lactoglobulin (betaLG) receptors on living murine hybridoma MARK-3 cells using a fluorescein isothiocyanate-betaLG conjugate (FITC-betaLG: molar ratio of 5:1). A site occupation curve was produced using a shift in the mean channel fluorescence at various conc...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Palupi NS,Franck P,Guimont C,Linden G,Dumas D,Stoltz J,Nabet P,Belleville-Nabet F,Dousset B

    更新日期:2000-03-10 00:00:00

  • Optical sequence probing with the homologous recombination protein RecA.

    abstract::In this study, we used the homologous recombination protein RecA to locate a specific sequence on DNA. Single-stranded (ss) DNA (80-mer, 5'-biotinylated), complementary to the sequence of interest, was labeled with quantum dots (Qdots(®)) via biotin-avidin binding. The DNA was then mixed with RecA to form a fluorescen...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kimura Y,Goto Y,Oana H,Washizu M

    更新日期:2012-12-15 00:00:00

  • On-line estimation of biomass, glucose and ethanol in Saccharomyces cerevisiae cultivations using in-situ multi-wavelength fluorescence and software sensors.

    abstract::Batch bioreactor cultivations using Saccharomyces cerevisiae at high (190-305 gl(-1) glucose) or low (21-25 gl(-1) glucose) gravity conditions were monitored on-line using multi-wavelength fluorescence (MWF) and standard monitoring sensors. Partial least squares models were calibrated for the prediction of cell dry we...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Odman P,Johansen CL,Olsson L,Gernaey KV,Lantz AE

    更新日期:2009-10-26 00:00:00

  • Rifampicin-resistance, rpoB polymorphism and RNA polymerase genetic engineering.

    abstract::Following its introduction in 1967, rifampicin has become a mainstay of therapy in the treatment of tuberculosis, leprosy and many other widespread diseases. Its potent antibacterial activity is due to specific inhibition of bacterial RNA polymerase. However, resistance to rifampicin was reported shortly after its int...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Alifano P,Palumbo C,Pasanisi D,Talà A

    更新日期:2015-05-20 00:00:00

  • Identification and analysis of the chivosazol biosynthetic gene cluster from the myxobacterial model strain Sorangium cellulosum So ce56.

    abstract::Myxobacteria belonging to the genus Sorangium are known to produce a variety of biologically active secondary metabolites. Chivosazol is a macrocyclic antibiotic active against yeast, filamentous fungi and especially against mammalian cells. The compound specifically destroys the actin skeleton of eucaryotic cells and...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Perlova O,Gerth K,Kaiser O,Hans A,Müller R

    更新日期:2006-01-24 00:00:00

  • Rapid and quantitative analysis of recombinant protein expression using pyrolysis mass spectrometry and artificial neural networks: application to mammalian cytochrome b5 in Escherichia coli.

    abstract::Recombinant Escherichia coli clones encoding between 0 and 6 copies of the mammalian cytochrome b5 gene were subjected to pyrolysis mass spectrometry (PyMS). To deconvolute the pyrolysis mass spectra so as to obtain quantitative information on the amount of cytochrome b5 produced fully-interconnected feedforward artif...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Goodacre R,Karim A,Kaderbhai MA,Kell DB

    更新日期:1994-05-15 00:00:00

  • Continuous production of lactulose by immobilized thermostable beta-glycosidase from Pyrococcus furiosus.

    abstract::A continuous enzymatic process for the production of the prebiotic disaccharide lactulose through transgalactosylation was developed using free and immobilized beta-glycosidase from Pyrococcus furiosus. The hyperthermostable beta-glycosidase (CelB) was immobilized onto an anion-exchange resin (Amberlite IRA-93) or ont...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Mayer J,Kranz B,Fischer L

    更新日期:2010-02-15 00:00:00

  • Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds.

    abstract::Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 microm) of macroporous poly(dimethylacrylamide) makes it possible to couple l...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Noppe W,Plieva FM,Vanhoorelbeke K,Deckmyn H,Tuncel M,Tuncel A,Galaev IY,Mattiasson B

    更新日期:2007-09-15 00:00:00

  • Improved activity and thermostability of Bacillus pumilus lipase by directed evolution.

    abstract::To improve enzymatic activity of Bacillus pumilus lipases, DNA shuffling was applied to two lipase genes from local B. pumilus isolates. Using a high-throughput activity assay, the mutant with highest activity was selected. This chimeric mutant (L3-3), carrying two crossover positions and three point mutations, has a ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Akbulut N,Tuzlakoğlu Öztürk M,Pijning T,İşsever Öztürk S,Gümüşel F

    更新日期:2013-03-10 00:00:00

  • Thermal stable and oxidation-resistant variant of subtilisin E.

    abstract::A remarkable thermal stable and oxidation-resistant mutant was obtained using the random mutagenesis PCR technique on the mutant M222A gene of subtilisin E. Sequencing analysis revealed an A was replaced by G at nucleotide 671 of the subtilisin E gene, converting the asparagine codon (AAT) to serine codon (AGT) at pos...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yang Y,Jiang L,Zhu L,Wu Y,Yang S

    更新日期:2000-08-25 00:00:00

  • Strong seed-specific protein expression from the Vigna radiata storage protein 8SGα promoter in transgenic Arabidopsis seeds.

    abstract::Vigna radiata (mung bean) is an important crop plant and is a major protein source in developing countries. Mung bean 8S globulins constitute nearly 90% of total seed storage protein and consist of three subunits designated as 8SGα, 8SGα' and 8SGβ. The 5'-flanking sequences of 8SGα' has been reported to confer high ex...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Chen MX,Zheng SX,Yang YN,Xu C,Liu JS,Yang WD,Chye ML,Li HY

    更新日期:2014-03-20 00:00:00

  • Improving thermal stability of thermophilic L-threonine aldolase from Thermotoga maritima.

    abstract::Threonine aldolase (TA) catalyzes a reversible reaction, in which threonine is decomposed into glycine and acetaldehyde. The same enzyme can be used to catalyze aldol reaction between glycine and a variety of aromatic and aliphatic aldehydes, thus creating various alpha-amino-alcohols. Therefore, TA is a very promisin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Wieteska L,Ionov M,Szemraj J,Feller C,Kolinski A,Gront D

    更新日期:2015-04-10 00:00:00

  • Exploiting the aglycon promiscuity of glycosyltransferase Bs-YjiC from Bacillus subtilis and its application in synthesis of glycosides.

    abstract::Glycosylation is a prominent biological mechanism for structural and functional diversity of natural products. Uridine diphosphate-dependent glycosyltransferases with aglycon promiscuity are generally recognised as effective biocatalysts for glycodiversification of natural products for practical applications. In this ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Dai L,Li J,Yao P,Zhu Y,Men Y,Zeng Y,Yang J,Sun Y

    更新日期:2017-04-20 00:00:00

  • Increased gene expression and inflammatory cell infiltration caused by electroporation are both important for improving the efficacy of DNA vaccines.

    abstract::One potential reason for the enhancement of immune responses to DNA vaccines following electroporation is increased gene expression. However, the inflammatory response and accompanying cellular infiltration stimulated by electroporation may also be essential for enhancing immune responses to DNA vaccines. These parame...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Babiuk S,Baca-Estrada ME,Foldvari M,Middleton DM,Rabussay D,Widera G,Babiuk LA

    更新日期:2004-05-13 00:00:00

  • Cross-linked enzyme aggregates of Mung bean epoxide hydrolases: a highly active, stable and recyclable biocatalyst for asymmetric hydrolysis of epoxides.

    abstract::A highly active and stable cross-linked enzyme aggregates (CLEAs) of epoxide hydrolases (EHs) from Mung bean, which plays a crucial role in synthesis of valuable enantiopure diols, were successfully prepared and characterized. Under the optimum preparation conditions, the activity recovery of CLEAs recorded 92%. The C...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Yu CY,Li XF,Lou WY,Zong MH

    更新日期:2013-06-20 00:00:00

  • Combining chitinase C and N-acetylhexosaminidase from Streptomyces coelicolor A3(2) provides an efficient way to synthesize N-acetylglucosamine from crystalline chitin.

    abstract::The enzymatic bioconversion of chitin is of considerable interest for the natural production of bioactive compounds such as chitooligosaccharides and N-acetyl-d-glucosamine (GlcNAc). Key enzymes are involved in the natural processing of chitin, hydrolyzing this abundant biopolymer to yield chitooligosaccharides with s...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Nguyen-Thi N,Doucet N

    更新日期:2016-02-20 00:00:00

  • Gene expression analysis using a modified HiCEP method applicable to prokaryotes: a study of the response of Rhodococcus to isoniazid and ethambutol.

    abstract::We established a novel method to analyze the gene expression of prokaryotes by modifying and optimizing the HiCEP (high coverage gene expression analysis) method, which was originally developed for eukaryotic gene expression profiling. Following the removal of abundant rRNA, the mRNA of prokaryotic cells was enzymatic...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Mitani Y,Suzuki K,Kondo K,Okumura K,Tamura T

    更新日期:2006-05-29 00:00:00

  • Selective enhancement of autotrophic acetate production with genetically modified Acetobacterium woodii.

    abstract::Great interest has emerged in the recent past towards the potential of autotrophic acetogenic bacteria for the sustainable production of fuels and chemicals. This group of microorganisms possesses an ancient pathway for the fixation of carbon dioxide in the presence of hydrogen, making them highly attractive for the u...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Straub M,Demler M,Weuster-Botz D,Dürre P

    更新日期:2014-05-20 00:00:00

  • Aptamer-nanobody based ELASA for specific detection of Acinetobacter baumannii isolates.

    abstract::Acinetobacter baumannii has turned into an important threat in nosocomial outbreak infections and multidrug resistance leading to high mortality rates in the 21st century. In recent years its mortality has increased by 15% which in part could be due to lack of a rapid and sensitive diagnostic test. In this work we int...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Rasoulinejad S,Gargari SLM

    更新日期:2016-08-10 00:00:00

  • Production of pharmaceutical proteins from transgenic animals.

    abstract::Different systems are being studied and used to prepare recombinant proteins for pharmaceutical use. The blood, and still more the milk, from transgenic animals appear a very attractive source of pharmaceuticals. The cells from these animals are expected to produce well-matured proteins in potentially huge amounts. Se...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Houdebine LM

    更新日期:1994-05-31 00:00:00

  • Clavibacter michiganensis subsp. michiganensis: first steps in the understanding of virulence of a Gram-positive phytopathogenic bacterium.

    abstract::Clavibacter michiganensis subsp. michiganensis is a plant-pathogenic actinomycete. It infects tomato, spreads through the xylem and causes bacterial wilt and canker. The wild-type strain NCPPB382 carries two plasmids, pCM1 and pCM2. The cured plasmid-free derivative CMM100 is still able to colonize tomato, but no dise...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Gartemann KH,Kirchner O,Engemann J,Gräfen I,Eichenlaub R,Burger A

    更新日期:2003-12-19 00:00:00

  • Development of an antibody control system using phage display.

    abstract::While chromatin immunoprecipitation has become a widely-used method in the field of transcription regulation studies, serious limitations connected to the complexity and relatively little standardization of the method serve as obstacles for its use in clinical research. In this paper we introduce a method for developi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Keresztessy Z,Erdos E,Ozgyin L,Kádas J,Horváth J,Zahuczky G,Balint BL

    更新日期:2019-07-20 00:00:00