Transduction of NeuroD2 protein induced neural cell differentiation.

Abstract:

:NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich domains, one is nuclear localization signal (NLS) and the other is basic region of basic helix-loop-helix (basic). We constructed some mutants of NeuroD2, ND2(Delta100-115) (lack of NLS), ND2(Delta123-134) (lack of basic) and ND2(Delta100-134) (lack of both NLS and basic) for transduction experiments. Using these proteins, we have shown that NLS region of NeuroD2 plays a role of protein transduction. Continuous addition of NeuroD2 protein resulted in N1E-115 cells adopting neural morphology after 4 days and Tau mRNA expression was increased. These results suggest that neural differentiation can be induced by direct addition of NeuroD2 protein.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Noda T,Kawamura R,Funabashi H,Mie M,Kobatake E

doi

10.1016/j.jbiotec.2006.04.021

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

230-6

issue

2

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(06)00336-1

journal_volume

126

pub_type

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