Abstract:
:NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich domains, one is nuclear localization signal (NLS) and the other is basic region of basic helix-loop-helix (basic). We constructed some mutants of NeuroD2, ND2(Delta100-115) (lack of NLS), ND2(Delta123-134) (lack of basic) and ND2(Delta100-134) (lack of both NLS and basic) for transduction experiments. Using these proteins, we have shown that NLS region of NeuroD2 plays a role of protein transduction. Continuous addition of NeuroD2 protein resulted in N1E-115 cells adopting neural morphology after 4 days and Tau mRNA expression was increased. These results suggest that neural differentiation can be induced by direct addition of NeuroD2 protein.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Noda T,Kawamura R,Funabashi H,Mie M,Kobatake Edoi
10.1016/j.jbiotec.2006.04.021subject
Has Abstractpub_date
2006-11-01 00:00:00pages
230-6issue
2eissn
0168-1656issn
1873-4863pii
S0168-1656(06)00336-1journal_volume
126pub_type
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