Abstract：
:A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were analyzed. The purified enzymes were classified with respect to their biochemical properties and substrate specificity. Among these proteins, one revealed polygalacturonase activity, another appeared to be a pectin methylesterase and three were identified as pectate lyases. The capacity of the fungus A. niger to produce pectate lyases with optimum pH in acidic domain was reported for the first time.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Dinu D,Nechifor MT,Stoian G,Costache M,Dinischiotu Adoi
10.1016/j.jbiotec.2007.06.005subject
Has Abstractpub_date
2007-08-31 00:00:00pages
128-37issue
2eissn
0168-1656issn
1873-4863pii
S0168-1656(07)00421-Xjournal_volume
131pub_type
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