Abstract：
:Aspergillus niger AB1.13 cultures with glucoamylase production (with D-glucose as substrate) and without glucoamylase production (with D-xylose as substrate) were characterized by metabolic flux analysis. Two comprehensive metabolic models for d-glucose- as well as for D-xylose-consumption were used to quantify and compare the metabolic fluxes through the central pathways of carbon metabolism at different pH-values. The models consist of the most relevant metabolic pathways for A. niger including glycolysis, pentose-phosphate pathway, citrate cycle, energy metabolism and anaplerotic reactions comprising two intracellular compartments, the cytoplasm and mitochondrion. When D-xylose was used as the sole carbon source, the relative flux of the substrate through the oxidative pentose-phosphate pathway (PPP) via G6P-dehydrogenase was unaffected by the pH-value of the culture medium. About 30% of D-xylose consumed was routed through the oxidative PPP. In contrast, the flux of D-glucose (i.e., under glucoamylase-producing conditions) through the oxidative PPP was remarkably higher and, in addition was significantly affected by the pH-value of the culture medium (40% at pH 5.5, 56% at pH 3.7, respectively). Summarizing, the flux through the PPP under glucoamylase producing conditions was 30-90% higher than for non-producing conditions.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Melzer G,Dalpiaz A,Grote A,Kucklick M,Göcke Y,Jonas R,Dersch P,Franco-Lara E,Nörtemann B,Hempel DCdoi
10.1016/j.jbiotec.2007.08.034subject
Has Abstractpub_date
2007-12-01 00:00:00pages
405-17issue
4eissn
0168-1656issn
1873-4863pii
S0168-1656(07)01525-8journal_volume
132pub_type
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