Abstract:
:An expression vector has been generated using a gene highly expressed under conditions found in a typical fed-batch bioreactor process. The ferritin heavy chain (HC) gene exhibits higher levels of expression in the late stages of a fed-batch bioreactor than in the early stages. This property was considered advantageous for an expression vector, since the maximal cell density would coincide with maximal expression. The rat ferritin HC genomic region was isolated and converted into an expression vector where large segments of 5' and 3' flanking regions were included in an attempt to recreate the same high level of expression in stably transfected cells. Expression from the resulting ferritin HC vector was compared to vectors containing the commonly used strong promoters, CMV IE, and SV40 early promoter/enhancer, in the generation of stable transfectants. The ferritin HC vector was able to generate cell lines with significantly higher expression levels than those under the control of the viral promoters.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Prentice HL,Tonkin CJ,Caamano L,Sisk WPdoi
10.1016/j.jbiotec.2006.09.021subject
Has Abstractpub_date
2007-01-30 00:00:00pages
50-60issue
1eissn
0168-1656issn
1873-4863pii
S0168-1656(06)00794-2journal_volume
128pub_type
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