High level expression of proteins using sequences from the ferritin heavy chain gene locus.

Abstract:

:An expression vector has been generated using a gene highly expressed under conditions found in a typical fed-batch bioreactor process. The ferritin heavy chain (HC) gene exhibits higher levels of expression in the late stages of a fed-batch bioreactor than in the early stages. This property was considered advantageous for an expression vector, since the maximal cell density would coincide with maximal expression. The rat ferritin HC genomic region was isolated and converted into an expression vector where large segments of 5' and 3' flanking regions were included in an attempt to recreate the same high level of expression in stably transfected cells. Expression from the resulting ferritin HC vector was compared to vectors containing the commonly used strong promoters, CMV IE, and SV40 early promoter/enhancer, in the generation of stable transfectants. The ferritin HC vector was able to generate cell lines with significantly higher expression levels than those under the control of the viral promoters.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Prentice HL,Tonkin CJ,Caamano L,Sisk WP

doi

10.1016/j.jbiotec.2006.09.021

subject

Has Abstract

pub_date

2007-01-30 00:00:00

pages

50-60

issue

1

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(06)00794-2

journal_volume

128

pub_type

杂志文章
  • Identification of the bacterial superoxide dismutase (SodM) as plant-inducible elicitor of an oxidative burst reaction in tobacco cell suspension cultures.

    abstract::Three of the most abundant proteins (OmpW, MopB and SodM) of the extracellular proteome of Xanthomonas campestris pv. campestris were analysed in a luminol-based oxidative burst assay to identify novel pathogen-associated molecular patterns (PAMP). Tobacco cell suspension cultures were used as a model system to monito...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2006.02.022

    authors: Watt SA,Tellström V,Patschkowski T,Niehaus K

    更新日期:2006-10-20 00:00:00

  • A novel direct screening method for alkyl glucoside production by glucosidases expressed in E. coli in 96-well plates.

    abstract::The present work describes the development of a novel direct screening method, assayed in 96-well format, for evaluation of enzymatic alkyl glycoside production in a hexanol-water two-phase system. Alkyl glycosides are surfactants with a range of applications and with good biodegradability and low toxicity. Enzymatic ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2009.11.005

    authors: Gräber M,Andersson M,Rundbäck F,Pozzo T,Karlsson EN,Adlercreutz P

    更新日期:2010-01-15 00:00:00

  • A Novel Method for Enhancing Strains' Biodegradation of 4-Chloronitrobenzene.

    abstract::This paper introduces a novel approach to enhance the strains' biodegradation of 4-chloronitrobenzene by utilizing the synergistic effect of the organic reductant mannitol and the substrate beef extraction. Our results demonstrate that 4-chloronitrobenzene could not be an available nitrogen source to support target st...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.10.005

    authors: Li T,Zhang TC,He L

    更新日期:2017-12-20 00:00:00

  • A fibrous-bed bioreactor for continuous production of developmental endothelial locus-1 by osteosarcoma cells.

    abstract::Genetically engineered human osteosarcoma cells containing developmental endothelial locus-1 (del-1) gene were studied for production of Del-1, a protein that has the properties of an extracellular matrix protein and can regulate vascular morphogenesis and remodeling. Del-1 has been studied as a potential anti-angioge...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(02)00053-6

    authors: Chen C,Huang YL,Yang ST

    更新日期:2002-07-17 00:00:00

  • Rapid and efficient galactose fermentation by engineered Saccharomyces cerevisiae.

    abstract::In the important industrial yeast Saccharomyces cerevisiae, galactose metabolism requires energy production by respiration; therefore, this yeast cannot metabolize galactose under strict anaerobic conditions. While the respiratory dependence of galactose metabolism provides benefits in terms of cell growth and populat...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.04.041

    authors: Quarterman J,Skerker JM,Feng X,Liu IY,Zhao H,Arkin AP,Jin YS

    更新日期:2016-07-10 00:00:00

  • De novo sequencing and transcriptome analysis of a low temperature tolerant Saccharum spontaneum clone IND 00-1037.

    abstract::Saccharum spontaneum L., a wild relative of sugarcane, is known for its adaptability to environmental stresses, particularly cold stress. In the present study, an attempt was made for transcriptome profiling of the low temperature (10°C) tolerant S. spontaneum clone IND 00-1037 collected from high altitude regions of ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.05.036

    authors: Dharshini S,Chakravarthi M,J AN,Manoj VM,Naveenarani M,Kumar R,Meena M,Ram B,Appunu C

    更新日期:2016-08-10 00:00:00

  • Characterization of stress and protein turnover from protein overexpression in fed-batch E. coli cultures.

    abstract::A structured kinetic model that accounts for proteolytic degradation due to recombinant protein overexpression is introduced and its performance evaluated by comparison with previously reported fed-batch experimental data. This mathematical model contains an additional pool for a generic key precursor (in our case phe...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(99)00014-0

    authors: Ramírez DM,Bentley WE

    更新日期:1999-05-28 00:00:00

  • 'Structural constraints in cyanobacteria-mediated whole-cell biotransformation of methoxylated and methylated derivatives of 2'-hydroxychalcone.

    abstract::Halophilic and freshwater strains of cyanobacteria representing the Oscillatoriales, Nostocales, Chroococcales, and Synechococcales orders of Cyanophyta were examined to determine (i) the resistance of their cultures when suppressed by the presence of exogenous methoxylated and methylated derivatives of 2'-hydroxychal...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2019.01.005

    authors: Żyszka-Haberecht B,Poliwoda A,Lipok J

    更新日期:2019-03-10 00:00:00

  • Use of flow-cytometric analysis to optimize cell banking strategies for production of biopharmaceuticals from mammalian cells.

    abstract::Production of biopharmaceuticals from mammalian cells requires generation of master, working and post-production cell banks of high quality under GMP conditions. An optimal cryopreservation strategy is needed for each new production cell line, particularly with regard to establishing production processes that are comp...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.05.023

    authors: Fieder J,Wagner N,Grammatikos S,Hoffmann H,Kaufmann H,Otto RT

    更新日期:2005-10-17 00:00:00

  • Elucidation of structure-function relationship of THCA and CBDA synthase from Cannabis sativaL.

    abstract::Cannabinoids are secondary natural products from the plant Cannabis sativaL. Therapeutic indications of cannabinoids currently comprise a significant area of medicinal research. We have expressed the Δ9-tetrahydrocannabinolic acid synthase (THCAS) and cannabidiolic acid synthase (CBDAS) recombinantly in Komagataella p...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2018.07.031

    authors: Zirpel B,Kayser O,Stehle F

    更新日期:2018-10-20 00:00:00

  • First complete genome sequence of Bacillus glycinifermentans B-27.

    abstract::The first complete genome sequence of Bacillus glycinifermentans B-27 was determined by SMRT sequencing generating a genome sequence with a total length of 4,607,442 bases. Based on this sequence 4738 protein-coding sequences were predicted and used to identify gene clusters that are related to the production of secon...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.04.021

    authors: Stadermann KB,Blom J,Borgmeier C,Sciberras N,Herbold S,Kipker M,Meurer G,Molck S,Petri D,Pelzer S,Schneider J

    更新日期:2017-09-10 00:00:00

  • Measurement of intracellular pH in cultured cells by flow cytometry with BCECF-AM.

    abstract::This study evaluates the suitability of flow cytometry with the fluorochrome BCECF for measuring the intracellular pH (pHi) of cultured cells, and monitors the changes in pHi in murine hybridoma in batch culture and chick embryo fibroblast in monolayer culture (5th passage). The technique produced highly reproducible,...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(95)00189-1

    authors: Franck P,Petitipain N,Cherlet M,Dardennes M,Maachi F,Schutz B,Poisson L,Nabet P

    更新日期:1996-05-15 00:00:00

  • Effects of the hydrodynamic environment and shear protectants on survival of erythrocytes in suspension.

    abstract::Survival of media-suspended porcine erythrocytes exposed to various hydrodynamic environments was investigated with and without such shear protectant additives as bovine serum albumin, dextran and the non-ionic surfactant Pluronic F68. Erythrocytes provided a model cell population with cells of a uniform size, metabol...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(95)00111-8

    authors: Zhang Z,Chisti Y,Moo-Young M

    更新日期:1995-11-21 00:00:00

  • Methods for reducing the ammonia in hybridoma cell cultures.

    abstract::The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(94)00142-y

    authors: Capiaumont J,Legrand C,Carbonell D,Dousset B,Belleville F,Nabet P

    更新日期:1995-02-21 00:00:00

  • Amperometric immunosensor based on toluidine blue/nano-Au through electrostatic interaction for determination of carcinoembryonic antigen.

    abstract::A new current amplified immunosensor for the determination of carcinoembryonic antigen (CEA) was demonstrated in this work. The electrode architecture was fabricated by positively charged toluidine blue (TB) coated on negatively charged poly-sulfanilic acid (PSAA) modified glassy carbon electrode (GCE) surface through...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.11.023

    authors: Li X,Yuan R,Chai Y,Zhang L,Zhuo Y,Zhang Y

    更新日期:2006-05-29 00:00:00

  • The effect of thermodynamic properties of solvent mixtures explains the difference between methanol and ethanol in C.antarctica lipase B catalyzed alcoholysis.

    abstract::Kinetic modelling, molecular modelling, and experimental determination of the initial reaction velocity of lipase-catalyzed alcoholysis were combined to study the effect of the alcohol substrate to catalytic activity. The model system consisted of methanol or ethanol at varying concentrations, vinyl acetate as ester s...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.08.023

    authors: Sasso F,Kulschewski T,Secundo F,Lotti M,Pleiss J

    更新日期:2015-11-20 00:00:00

  • Direct oligonucleotide synthesis onto super-paramagnetic beads.

    abstract::Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To mainstream the process, we describe a novel procedure of direct oligon...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.08.006

    authors: Jensen MA,Akhras MS,Fukushima M,Pourmand N,Davis RW

    更新日期:2013-09-20 00:00:00

  • Synthesis and characterization of a new cutinase substrate, 4-nitrophenyl (16-methyl sulfone ester) hexadecanoate.

    abstract::Phytopathogenic fungi penetrate plants by breaking down the cuticular barrier with cutinase. Cutinases are extracellular hydrolytic enzymes that degrade cutin, a polyester composed of hydroxy and epoxy fatty acids. Until now, cutinase has been recognized by its ability to release labeled cutin monomers or by a non-spe...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.08.011

    authors: Degani O,Salman H,Gepstein S,Dosoretz CG

    更新日期:2006-02-10 00:00:00

  • Process stability and microbial community structure in anaerobic hydrogen-producing microflora from food waste containing kimchi.

    abstract::Hydrogen production by the dark fermentation of food wastes is an economic and environmentally friendly technology to produce the clean energy source as well as to treat the problematic wastes. However, the long-term operations of the continuous anaerobic reactor for fermentative hydrogen production were frequently un...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2007.07.492

    authors: Jo JH,Jeon CO,Lee DS,Park JM

    更新日期:2007-09-15 00:00:00

  • Cell growth and enzyme synthesis of a mutant of Arthrobacter sp. (DSM 3747) used for the production of L-amino acids from D,L-5-monosubstituted hydantoins.

    abstract::A microorganism with the ability to form L-tryptophan from D,L-5-(3-indolyl-methyl)hydantoin (D,L-5-IMH) was isolated and identified as Arthrobacter sp. (DSM 3747). After isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high am...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(90)90118-u

    authors: Syldatk C,Mackowiak V,Höke H,Gross C,Dombach G,Wagner F

    更新日期:1990-06-01 00:00:00

  • Bioelectrocatalysis: the electrochemical kinetics of hydrogenase action.

    abstract::A hydrogen electrode based on a hydrogenase immobilized on a carbon material has been developed. The equilibrium hydrogen potential was reached in an atmosphere of H2 on the enzyme electrode according to the mechanism of direct exchange of electrons between the electrode and the enzyme active center. The electrochemic...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/0168-1656(93)90095-5

    authors: Varfolomeyev SD,Yaropolov AI,Karyakin AA

    更新日期:1993-02-01 00:00:00

  • Chemistry and applications of oligonucleotide analogues.

    abstract::This review is aimed at biochemists and molecular biologists, and covers the chemistry and key features involved in the solid-phase synthesis of a variety of the better known DNA and RNA analogues by the phosphoramidite and H-phosphonate methods. A wide spectrum of biological applications such as inhibition of gene ex...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/0168-1656(95)00012-f

    authors: Sproat BS

    更新日期:1995-07-31 00:00:00

  • Metabolic flux analysis of Escherichia coli K12 grown on 13C-labeled acetate and glucose using GC-MS and powerful flux calculation method.

    abstract::A new algorithm was developed for the estimation of the metabolic flux distribution based on GC-MS data of proteinogenic amino acids. By using a sensitive GC-MS protocol as well as by combining the global search algorithm such as the genetic algorithm with the local search algorithm such as the Levenberg-Marquardt alg...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(02)00316-4

    authors: Zhao J,Shimizu K

    更新日期:2003-03-06 00:00:00

  • Intracellular glucose 6-phosphate content in Streptomyces coelicolor upon environmental changes in a defined medium.

    abstract::A new, chemically defined medium providing dispersed growth and high biomass formation and a method for quantitative extraction of intracellular metabolites was used to investigate the cellular response of Streptomyces coelicolor A3(2) during growth and upon changes in nutrient utilization. Fast changes of the glucose...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(99)00215-1

    authors: Mira de Orduña R,Theobald U

    更新日期:2000-02-17 00:00:00

  • Large double copy vectors are functional but show a size-dependent decline in transduction efficiency.

    abstract::The development of SIN-lentivectors has paved the way for the double copy vectors (DCV), which substitute the deletion in the 3'LTR with either transgenic or insulator sequences. However, the limits of this approach remain unclear. Previous results have demonstrated that transduction efficiencies of DCV carrying large...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2010.07.010

    authors: Bos TJ,De Bruyne E,Van Lint S,Heirman C,Vanderkerken K

    更新日期:2010-10-01 00:00:00

  • Engineering microenvironment for expansion of sensitive anchorage-dependent mammalian cells.

    abstract::Tissue engineering involves ex vivo seeding of anchorage-dependent mammalian cells onto scaffolds, or transplanting cells in vivo. The cell expansion currently requires repeated cell detachment from solid substrata by enzymatic, chemical or mechanical means. The report here presents a high yield three-dimensional cult...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.05.012

    authors: Chia SM,Lin PC,Quek CH,Yin C,Mao HQ,Leong KW,Xu X,Goh CH,Ng ML,Yu H

    更新日期:2005-09-10 00:00:00

  • Engineering of Corynebacterium glutamicum for minimized carbon loss during utilization of D-xylose containing substrates.

    abstract::Biomass-derived d-xylose represents an economically interesting substrate for the sustainable microbial production of value-added compounds. The industrially important platform organism Corynebacterium glutamicum has already been engineered to grow on this pentose as sole carbon and energy source. However, all current...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.09.026

    authors: Radek A,Krumbach K,Gätgens J,Wendisch VF,Wiechert W,Bott M,Noack S,Marienhagen J

    更新日期:2014-12-20 00:00:00

  • Use of adapted Aspergillus niger in the bioleaching of spent refinery processing catalyst.

    abstract::Spent refinery processing catalyst is listed as a hazardous waste; the toxicity characteristic leaching procedure (TCLP) extracts of the catalyst are found to contain heavy metals at concentrations exceeding the regulated levels. In the present investigation, Aspergillus niger was adapted to single metal ions Ni, Mo o...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.07.002

    authors: Santhiya D,Ting YP

    更新日期:2006-01-02 00:00:00

  • Polyhydroxyalkanoate (PHA) synthase genes and PHA-associated gene clusters in Pseudomonas spp. and Janthinobacterium spp. isolated from Antarctica.

    abstract::The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacter...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2020.03.006

    authors: Tan IKP,Foong CP,Tan HT,Lim H,Zain NA,Tan YC,Hoh CC,Sudesh K

    更新日期:2020-04-10 00:00:00

  • Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris.

    abstract::The methylotropic yeast Pichia pastoris has demonstrated its suitability for large-scale production of recombinant proteins. As an eukaryotic organism P. pastoris presents a series of advantages at expression and processing of heterologous proteins when compared with Escherichia coli. In this work, P. pastoris has bee...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.04.024

    authors: de la Cruz S,Alcocer M,Madrid R,García A,Martín R,González I,García T

    更新日期:2016-06-10 00:00:00