当前位置： SCI文献检索 > JOURNAL OF BIOTECHNOLOGY期刊下所有文献 > Engineering biotin prototrophic Corynebacterium glutamicum strains for amino acid, diamine and carotenoid production.

Engineering biotin prototrophic Corynebacterium glutamicum strains for amino acid, diamine and carotenoid production.

Abstract：

:The Gram-positive Corynebacterium glutamicum is auxotrophic for biotin. Besides the biotin uptake system BioYMN and the transcriptional regulator BioQ, this bacterium possesses functional enzymes for the last three reactions of biotin synthesis starting from pimeloyl-CoA. Heterologous expression of bioF from the Gram-negative Escherichia coli enabled biotin synthesis from pimelic acid added to the medium, but expression of bioF together with bioC and bioH from E. coli did not entail biotin prototrophy. Heterologous expression of bioWAFDBI from Bacillus subtilis encoding another biotin synthesis pathway in C. glutamicum allowed for growth in biotin-depleted media. Stable growth of the recombinant was observed without biotin addition for eight transfers to biotin-depleted medium while the empty vector control stopped growth after the first transfer. Expression of bioWAFDBI from B. subtilis in C. glutamicum strains overproducing the amino acids l-lysine and l-arginine, the diamine putrescine, and the carotenoid lycopene, respectively, enabled formation of these products under biotin-depleted conditions. Thus, biotin-prototrophic growth and production by recombinant C. glutamicum were achieved.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Peters-Wendisch P,Götker S,Heider SA,Komati Reddy G,Nguyen AQ,Stansen KC,Wendisch VF

doi

10.1016/j.jbiotec.2014.01.023

subject

Has Abstract

pub_date

2014-12-20 00:00:00

pages

346-54

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(14)00040-6

journal_volume

192 Pt B

pub_type

杂志文章

相关文献

JOURNAL OF BIOTECHNOLOGY文献大全
  • A novel approach for the construction of plant amiRNA expression vectors.

    abstract：:Artificial microRNA (amiRNA) technology has been applied in Arabidopsis thaliana and other plants to efficiently silence target genes of interest. Here we described a novel approach to construct plant amiRNA expression vectors with seamless enzyme-free cloning (SEFC) and mating-assisted genetically integrated cloning ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2010.10.078

    authors： Yan H,Deng X,Cao Y,Huang J,Ma L,Zhao B

    更新日期：2011-01-10 00:00:00

  • Maximizing microbial degradation of perchlorate using a genetic algorithm: Media optimization.

    abstract：:Microbial communities are under constant influence of physical and chemical components in ecosystems. Shifts in conditions such as pH, temperature or carbon source concentration can translate into shifts in overall ecosystem functioning. These conditions can be manipulated in a laboratory setup using evolutionary comp...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.10.011

    authors： Kucharzyk KH,Crawford RL,Paszczynski AJ,Soule T,Hess TF

    更新日期：2012-01-01 00:00:00

  • Continuous cultivation start-up control--an experimental investigation.

    abstract：:A control strategy to avoid development of synchronous growth in carbohydrate limited Saccharomyces cerevisiae cultivations is proposed and experimentally investigated. The basic idea is to control the metabolic flux through the pathways by manipulating the substrate feed rate to keep the ethanol concentration at a lo...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(97)01662-3

    authors： Møller H,Jørgensen SB

    更新日期：1997-02-28 00:00:00

  • Metabolic flux analysis using stoichiometric models for Aspergillus niger: comparison under glucoamylase-producing and non-producing conditions.

    abstract：:Aspergillus niger AB1.13 cultures with glucoamylase production (with D-glucose as substrate) and without glucoamylase production (with D-xylose as substrate) were characterized by metabolic flux analysis. Two comprehensive metabolic models for d-glucose- as well as for D-xylose-consumption were used to quantify and co...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.08.034

    authors： Melzer G,Dalpiaz A,Grote A,Kucklick M,Göcke Y,Jonas R,Dersch P,Franco-Lara E,Nörtemann B,Hempel DC

    更新日期：2007-12-01 00:00:00

  • Development of an ELP-Z based mAb affinity precipitation process using scaled-down filtration techniques.

    abstract：:In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based monoclonal antibody (mAb) affinity precipitation process is developed using scaled-down filtration techniques. Tangential flow filtration (TFF) is examined for the recovery of ELP-Z-mAb precipitates formed during the mAb binding st...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.09.020

    authors： Sheth RD,Bhut BV,Jin M,Li Z,Chen W,Cramer SM

    更新日期：2014-12-20 00:00:00

  • Two endoxylanases active and stable at alkaline pH from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099.

    abstract：:Two extra-cellular endoxylanases (Xyl Ia and Ib) were purified to homogeneity from the newly isolated thermophilic fungus, Myceliophthora sp. IMI 387099. Xyl Ia and Ib, having a molecular mass of approximately 53 kDa and pI of 5.2 and 4.8, respectively, were optimally active at 75 degrees C and at pH 6.0. They were st...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2003.12.010

    authors： Chadha BS,Ajay BK,Mellon F,Bhat MK

    更新日期：2004-04-29 00:00:00

  • Recent developments in the structural organization and regulation of nitrogen fixation genes in Herbaspirillum seropedicae.

    abstract：:Herbaspirillum seropedicae is a nitrogen-fixing bacterium found in association with economically important gramineae. Regulation of nitrogen fixation involves the transcriptional activator NifA protein. The regulation of NifA protein and its truncated mutant proteins is described and compared with that of other nitrog...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1656(01)00343-1

    authors： Pedrosa FO,Benelli EM,Yates MG,Wassem R,Monteiro RA,Klassen G,Steffens MB,Souza EM,Chubatsu LS,Rigo LU

    更新日期：2001-10-04 00:00:00

  • Self-cloning in filamentous fungi: application to the construction of endothiapepsin overproducers in Cryphonectria parasitica.

    abstract：:The filamentous ascomycete fungus Cryphonectria parasitica naturally secretes endothiapepsin, an aspartic proteinase. It is cultured on a commercial scale as a source of the milk-clotting enzyme for cheese making. Our objective was to increase enzyme production of an industrial C. parasitica strain by a new technique ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(95)00036-p

    authors： Jara P,Delmas P,Razanamparany V,Olsen L,Dupin P,Bayol A,Bégueret J,Loison G

    更新日期：1995-06-01 00:00:00

  • Development of an antibody control system using phage display.

    abstract：:While chromatin immunoprecipitation has become a widely-used method in the field of transcription regulation studies, serious limitations connected to the complexity and relatively little standardization of the method serve as obstacles for its use in clinical research. In this paper we introduce a method for developi...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2019.05.009

    authors： Keresztessy Z,Erdos E,Ozgyin L,Kádas J,Horváth J,Zahuczky G,Balint BL

    更新日期：2019-07-20 00:00:00

  • Microbial community diversity of organically rich cassava sago factory waste waters and their ability to use nitrate and N2O added as external N-sources for enhancing biomethanation and the purification efficiency.

    abstract：:Water shortage necessitated South Indian sago factory owners, extracting starch out of cassava tubers, to install biogas plants where a starch utilizing microbial community multiplies and reduces the biological oxygen demand (BOD) of the waste waters by presently about 30%. The purification efficiency of sago factory ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.11.013

    authors： Rajendran R,Soora M,Dananjeyan B,Ratering S,Krishnamurthy K,Benckiser G

    更新日期：2012-12-15 00:00:00

  • The effect of nitrate and ammonium concentrations on growth and alkaloid accumulation of Atropa belladonna hairy roots.

    abstract：:The growth, the alkaloid production, as well as the scopolamine/hyoscyamine ratio of two clones of belladonna hairy roots were studied. The effects of nitrate and ammonium concentrations on these cultures were investigated. A rise in ammonium concentration caused the decline of the hairy roots, while nitrate had a mar...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(00)00372-2

    authors： Bensaddek L,Gillet F,Saucedo JE,Fliniaux MA

    更新日期：2001-01-23 00:00:00

  • Regulation of the pigment optical density of an algal cell: filling the gap between photosynthetic productivity in the laboratory and in mass culture.

    abstract：:An increasing number of investors is looking at algae as a viable source of biofuels, beside cultivation for human/animal feeding or to extract high-value chemicals and pharmaceuticals. However, present biomass productivities are far below theoretical estimations implying that a large part of the available photosynthe...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.02.021

    authors： Formighieri C,Franck F,Bassi R

    更新日期：2012-11-30 00:00:00

  • Bacterial xylanase expression in mammalian cells and transgenic mice.

    abstract：:The energy which simple-stomached livestock can derive from dietary plant material is limited by the lack of plant polysaccharide degrading enzymes in their gastro-intestinal (GI) tract and the inefficient microbial fermentation of such material in their hind-gut. In poultry the non-starch polysaccharides found in cer...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(99)00098-x

    authors： Fontes CM,Ali S,Gilbert HJ,Hazlewood GP,Hirst BH,Hall J

    更新日期：1999-06-11 00:00:00

  • Site-specific tetrameric streptavidin-protein conjugation using sortase A.

    abstract：:Streptavidin is tetrameric protein which has tight and specific biotin binding affinity, and streptavidin modification of proteins or small molecules is widely used for biotechnology tool. Here, we demonstrate site-specific streptavidin-protein conjugation using enzymes. We focused on sortase A, a transpeptidase from ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.01.008

    authors： Matsumoto T,Sawamoto S,Sakamoto T,Tanaka T,Fukuda H,Kondo A

    更新日期：2011-03-10 00:00:00

  • Engineering a repression-free catabolite-enhanced expression system for a thermophilic alpha-amylase from Bacillus licheniformis MSG.

    abstract：:Most expression systems used for the over production of many enzymes employ carbon catabolite repressible promoters and hence must use sugar free, rich complex media. Use of expression systems to overcome carbon repression opens an avenue for exploiting cheap carbon sources for the production of recombinant enzymes. A...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.09.016

    authors： Nathan S,Nair M

    更新日期：2013-12-01 00:00:00

  • Multi-assignment clustering: Machine learning from a biological perspective.

    abstract：:A common approach for analyzing large-scale molecular data is to cluster objects sharing similar characteristics. This assumes that genes with highly similar expression profiles are likely participating in a common molecular process. Biological systems are extremely complex and challenging to understand, with proteins...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2020.12.002

    authors： Ulfenborg B,Karlsson A,Riveiro M,Andersson CX,Sartipy P,Synnergren J

    更新日期：2021-01-20 00:00:00

  • The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP.

    abstract：:Using an RT-PCR approach a cDNA clone, designated Ms-Rac4 and putatively coding for a small GTPase was isolated from Medicago sativa. Ms-Rac4 and the earlier described Ms-Rac1 [Mol. Gen. Genet. 263 (2000) 761] belong to the class of GTP-binding Rho of plants (Rop) proteins. At the amino acid level they display all con...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2004.04.030

    authors： Brecht M,Sewald K,Schiene K,Keen G,Fricke M,Sauer M,Niehaus K

    更新日期：2004-08-26 00:00:00

  • New N-acyl-D-glucosamine 2-epimerases from cyanobacteria with high activity in the absence of ATP and low inhibition by pyruvate.

    abstract：:N-Acetylneuraminic acid, an important component of glycoconjugates with various biological functions, can be produced from N-acetyl-D-glucosamine (GlcNAc) and pyruvate using a one-pot, two-enzyme system consisting of N-acyl-D-glucosamine 2-epimerase (AGE) and N-acetylneuraminate lyase (NAL). In this system, the epimer...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2013.07.003

    authors： Klermund L,Groher A,Castiglione K

    更新日期：2013-11-01 00:00:00

  • An alpha-L-arabinofuranosidase from Penicillium purpurogenum: production, purification and properties.

    abstract：:Penicillium purpurogenum secretes arabinofuranosidase to the growth medium. Highest levels of enzyme (1.0 U ml(-1)) are obtained when L-arabitol is used as carbon source, while 0.85 and 0.7 U ml(-1) are produced with sugar beet pulp and oat spelts xylan, respectively. By means of a zymogram, three bands with arabinofu...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(99)00190-x

    authors： De Ioannes P,Peirano A,Steiner J,Eyzaguirre J

    更新日期：2000-01-21 00:00:00

  • A repeat-batch membrane bioreactor with a phase inversion for the desaturation of isopropyl palmitate by a mutant Rhodococcus strain.

    abstract：:A repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a Rhodococcus mutant. The addition of glutamate, thiamine, and MgSO(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(00)00255-8

    authors： Koike K,Takeuchi K,Mino H,Takaiwa M,Tohoh T,Tadokoro T,Tsutoh K,Ito S

    更新日期：2000-06-23 00:00:00

  • Engineering microenvironment for expansion of sensitive anchorage-dependent mammalian cells.

    abstract：:Tissue engineering involves ex vivo seeding of anchorage-dependent mammalian cells onto scaffolds, or transplanting cells in vivo. The cell expansion currently requires repeated cell detachment from solid substrata by enzymatic, chemical or mechanical means. The report here presents a high yield three-dimensional cult...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.05.012

    authors： Chia SM,Lin PC,Quek CH,Yin C,Mao HQ,Leong KW,Xu X,Goh CH,Ng ML,Yu H

    更新日期：2005-09-10 00:00:00

  • Comparative characterization of a recombinant Volvariella volvacea endoglucanase I (EG1) with its truncated catalytic core (EG1-CM), and their impact on the bio-treatment of cellulose-based fabrics.

    abstract：:Recombinant Volvariella volvacea endoglucanase 1 (EG1) and its catalytic module (EG1-CM) were obtained by expression in Pichia pastoris, purified by two-step chromatography, and the catalytic activities and binding capacities were compared. EG1 and EG1-CM exhibited very similar specific activities towards the soluble ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.05.016

    authors： Wu S,Ding S,Zhou R,Li Z

    更新日期：2007-07-15 00:00:00

  • Urinary-exosomal miR-2909: A novel pathognomonic trait of prostate cancer severity.

    abstract：:The global occurrence of prostate cancer with a range of patient outcome has prompted various investigators to explore novel molecular biomarkers that can precisely detect and track this type of cancer severity. Several studies suggest that micro-RNAs have emerged to act as a new largely unexplored class of biomarkers...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2017.07.029

    authors： Wani S,Kaul D,Mavuduru RS,Kakkar N,Bhatia A

    更新日期：2017-10-10 00:00:00

  • The BIORACK facility and its performance during the IML-2 Spacelab mission.

    abstract：:The configuration and performance of the Biorack facility during the Second International Microgravity Laboratory mission (IML-2; 8-23 July 1995) is described in detail. During this Spacelab mission, Biorack flew with two incubators (22 degrees C and 37 degrees C), glovebox, cooler (5 degrees C) and four passive ther...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(96)01365-x

    authors： Manieri P,Brinckmann E,Brillouet C

    更新日期：1996-06-27 00:00:00

  • Characterisation of non-porous magnetic chelator supports and their use to recover polyhistidine-tailed T4 lysozyme from a crude E. coli extract.

    abstract：:The use of high capacity micron-sized non-porous magnetic metal chelator adsorbents for the direct recovery of a recombinant metal-binding protein from crude liquors is described. Selectivity and interaction strength of magnetic chelator particles were assessed using a set of native proteins with known behaviour towar...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(97)01688-x

    authors： O'Brien SM,Sloane RP,Thomas OR,Dunnill P

    更新日期：1997-04-04 00:00:00

  • Antigen handling in the gastrointestinal lamina propria.

    abstract：:Enteric infections associated with bacteria, viruses and parasites are a world health problem accounting for at least one billion episodes of diarrhoea and up to 10 million deaths in the Third World each year. The widely held conclusion that responses which are protective in the gastrointestinal tract are best stimula...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/0168-1656(95)00114-X

    authors： Stokes CR,Bailey M

    更新日期：1996-01-26 00:00:00

  • Process stability and microbial community structure in anaerobic hydrogen-producing microflora from food waste containing kimchi.

    abstract：:Hydrogen production by the dark fermentation of food wastes is an economic and environmentally friendly technology to produce the clean energy source as well as to treat the problematic wastes. However, the long-term operations of the continuous anaerobic reactor for fermentative hydrogen production were frequently un...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.07.492

    authors： Jo JH,Jeon CO,Lee DS,Park JM

    更新日期：2007-09-15 00:00:00

  • Co-expression of disulfide oxidoreductases DsbA/DsbC markedly enhanced soluble and functional expression of reteplase in Escherichia coli.

    abstract：:Reteplase is the third generation of thrombolytic medicine and has many advantages over commercial t-PA. However, over-expressing recombinant reteplase in E. coli always accumulates as inclusion bodies due to nine pairs of disulfide bonds formation that is the main obstacle for correct folding. In this paper, in order...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.10.028

    authors： Zhuo XF,Zhang YY,Guan YX,Yao SJ

    更新日期：2014-12-20 00:00:00

  • Active fusions of Cas9 orthologs.

    abstract：:Many recent epigenetic studies utilize the advantages of CRISPR/dCas9 based tools in linking certain epigenetic modification with gene expression regulation. Various multifactorial diseases often contain changed epigenetic signatures at many loci, so tools for simultaneously targeting different loci would significantl...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2019.05.306

    authors： Josipović G,Zoldoš V,Vojta A

    更新日期：2019-08-10 00:00:00

  • Rewiring the reductive tricarboxylic acid pathway and L-malate transport pathway of Aspergillus oryzae for overproduction of L-malate.

    abstract：:Aspergillus oryzae finds wide application in the food, feed, and wine industries, and is an excellent cell factory platform for production of organic acids. In this work, we achieved the overproduction of L-malate by rewiring the reductive tricarboxylic acid (rTCA) pathway and L-malate transport pathway of A. oryzae N...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2017.05.011

    authors： Liu J,Xie Z,Shin HD,Li J,Du G,Chen J,Liu L

    更新日期：2017-07-10 00:00:00