A novel approach for the construction of plant amiRNA expression vectors.

Abstract:

:Artificial microRNA (amiRNA) technology has been applied in Arabidopsis thaliana and other plants to efficiently silence target genes of interest. Here we described a novel approach to construct plant amiRNA expression vectors with seamless enzyme-free cloning (SEFC) and mating-assisted genetically integrated cloning (MAGIC). Two pairs of primers were designed when the loop of amiRNA precursor was longer than 60 bp while three oligonucleotides were used to amplify the linearized vector containing the amiRNA precursor whose loop was smaller than 60 bp. The PCR products were transformed into Escherichia coli to generate the donor plasmid containing the amiRNA expression cassette through homologous recombination in vivo. The amiRNA expression cassette was then transferred to the recipient plasmid via MAGIC and an amiRNA expression plasmid was created. More than 200 amiRNA expression vectors were generated with this approach, three of which have been transformed into A. thaliana and successfully silence the target genes. Given its low-cost and simplicity, this novel approach of plant amiRNA expression vectors construction will benefit the study of individual gene function and establishment of plant amiRNA libraries.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Yan H,Deng X,Cao Y,Huang J,Ma L,Zhao B

doi

10.1016/j.jbiotec.2010.10.078

subject

Has Abstract

pub_date

2011-01-10 00:00:00

pages

9-14

issue

1

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(10)01992-9

journal_volume

151

pub_type

杂志文章
  • Coupled production in biorefineries--combined use of biomass as a source of energy, fuels and materials.

    abstract::In spite of high prices for fossil raw materials the production of biomass-based products is rarely economically successful today. Depending on the location feedstock prices are currently so high that products from renewable resources are not marketable when produced in existing process chains. Apart from the higher f...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2009.03.016

    authors: Lyko H,Deerberg G,Weidner E

    更新日期:2009-06-01 00:00:00

  • Effect of lhcsr gene dosage on oxidative stress and light use efficiency by Chlamydomonas reinhardtii cultures.

    abstract::Unicellular green algae, a promising source for renewable biofuels, produce lipid-rich biomass from light and CO2. Productivity in photo-bioreactors is affected by inhomogeneous light distribution from high cell pigment causing heat dissipation of light energy absorbed in excess and shading of the deep layers. Contras...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2020.12.023

    authors: Barera S,Dall'Osto L,Bassi R

    更新日期:2021-01-09 00:00:00

  • Hydrophobic properties conferred to Kraft pulp by a laccase-catalysed treatment with lauryl gallate.

    abstract::Hydrophobic properties were conferred to a high-lignin-content Kraft pulp by a laccase-catalysed treatment in the presence of lauryl gallate (LG). The treatment resulted in a two-fold increase in contact angle and conferred water absorption resistance to the pulp. Kappa number was increased, indicating that some pheno...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.07.014

    authors: Reynaud C,Tapin-Lingua S,Elegir G,Petit-Conil M,Baumberger S

    更新日期:2013-09-10 00:00:00

  • Stability and stabilization of globular proteins in solution.

    abstract::Proteins are multifunctional: their amino acid sequences simultaneously determine folding, function and turnover. Correspondingly, evolution selected for compromises between rigidity (stability) and flexibility (folding/function/degradation), to the result that generally the free energy of stabilization of globular pr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/s0168-1656(00)00236-4

    authors: Jaenicke R

    更新日期:2000-05-26 00:00:00

  • Deciphering molecular consequences of the proprotein convertase 1/3 inhibition in macrophages for application in anti-tumour immunotherapy.

    abstract::During tumour development, macrophages are recruited to the tumour site and orientated towards an anti-inflammatory phenotype. Due to their immunosuppressive function, tumour associated macrophages (TAMs) are recognized as major components in tumour progression. Changing these macrophages to a pro-inflammatory phenoty...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2018.07.002

    authors: Rodet F,Capuz A,Hara T,van Meel R,Duhamel M,Rose M,Raffo-Romero A,Fournier I,Salzet M

    更新日期:2018-09-20 00:00:00

  • Overexpression of GRAS Rhizomucor miehei lipase in Yarrowia lipolytica via optimizing promoter, gene dosage and fermentation parameters.

    abstract::Rhizomucor miehei lipase (RML), a GRAS catalyst with wide applications, was overexpressed in Yarrowia lipolytica, also a GRAS unconventional yeast, via a combined strategy, optimization for promoter, gene dosage and fermentation process. The lipase activity of the recombinant strain was first increased from 19.5 to 26...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2019.09.004

    authors: Zhou Q,Jiao L,Qiao Y,Wang Y,Xu L,Yan J,Yan Y

    更新日期:2019-12-20 00:00:00

  • Mass production of somatic embryos expressing Escherichia coli heat-labile enterotoxin B subunit in Siberian ginseng.

    abstract::The B subunit of Escherichia coli heat-labile toxin (LTB) is a potent mucosal immunogen and immunoadjuvant for co-administered antigens. In order to produce large scale of LTB for the development of edible vaccine, we used transgenic somatic embryos of Siberian ginseng, which is known as medicinal plant. When transgen...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.07.020

    authors: Kang TJ,Lee WS,Choi EG,Kim JW,Kim BG,Yang MS

    更新日期:2006-01-24 00:00:00

  • A new molecular approach based on qPCR for the quantification of fecal bacteria in contaminated marine sediments.

    abstract::Harbour sediments are periodically subjected to dredging operations and their management is mainly based on the assessment of the chemical contamination levels, but the potential risks posed by the presence of pathogenic microorganisms have been largely neglected. Here we first developed new molecular protocols based ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2011.07.033

    authors: Luna GM,Dell'Anno A,Pietrangeli B,Danovaro R

    更新日期:2012-02-20 00:00:00

  • Polyhydroxyalkanoate (PHA) synthase genes and PHA-associated gene clusters in Pseudomonas spp. and Janthinobacterium spp. isolated from Antarctica.

    abstract::The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacter...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2020.03.006

    authors: Tan IKP,Foong CP,Tan HT,Lim H,Zain NA,Tan YC,Hoh CC,Sudesh K

    更新日期:2020-04-10 00:00:00

  • Enhanced production of 2'-fucosyllactose in engineered Escherichia coli BL21star(DE3) by modulation of lactose metabolism and fucosyltransferase.

    abstract::2'-Fucosyllactose (2-FL) is one of most abundant functional oligosaccharides in human milk, which is involved in many biological functions for human health. To date, most microbial systems for 2-FL production have been limited to use Escherichia coli JM strains since they cannot metabolize lactose. In this study, E. c...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.06.431

    authors: Chin YW,Kim JY,Lee WH,Seo JH

    更新日期:2015-09-20 00:00:00

  • Effective delivery of anti-miRNA DNA oligonucleotides by functionalized gold nanoparticles.

    abstract::MicroRNAs (miRNAs) are gaining recognition as essential regulators involved in many biological processes, and they are emerging as therapeutic targets for treating disease. Here, we introduce a method for effective delivery of anti-miRNA oligonucleotides (AMOs) using functionalized gold nanoparticles (AuNPs). To demon...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2011.07.014

    authors: Kim JH,Yeom JH,Ko JJ,Han MS,Lee K,Na SY,Bae J

    更新日期:2011-09-20 00:00:00

  • Effects of redox buffer properties on the folding of a disulfide-containing protein: dependence upon pH, thiol pKa, and thiol concentration.

    abstract::Aliphatic thiols are effective as redox buffers for folding non-native disulfide-containing proteins into their native state at high pH values (8.0-8.5) but not at neutral pH values (6-7.5). In developing more efficient and flexible redox buffers, a series of aromatic thiols was analyzed for its ability to fold scramb...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2004.09.005

    authors: Gough JD,Lees WJ

    更新日期:2005-02-09 00:00:00

  • Enhanced production of β-glucosides by in-situ UDP-glucose regeneration.

    abstract::Glycosyltransferase (GT)-mediated methodology is recognized as one of the most practical approaches for large-scale production of glycosides. However, GT enzymes require a sugar nucleotide as donor substrate that must be generated in situ for preparative applications by recycling of the nucleotide moiety, e.g. by sucr...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.02.022

    authors: Huang FC,Hinkelmann J,Hermenau A,Schwab W

    更新日期:2016-04-20 00:00:00

  • Strong seed-specific protein expression from the Vigna radiata storage protein 8SGα promoter in transgenic Arabidopsis seeds.

    abstract::Vigna radiata (mung bean) is an important crop plant and is a major protein source in developing countries. Mung bean 8S globulins constitute nearly 90% of total seed storage protein and consist of three subunits designated as 8SGα, 8SGα' and 8SGβ. The 5'-flanking sequences of 8SGα' has been reported to confer high ex...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.01.027

    authors: Chen MX,Zheng SX,Yang YN,Xu C,Liu JS,Yang WD,Chye ML,Li HY

    更新日期:2014-03-20 00:00:00

  • Characterization of a Trichoplusia ni hexamerin-derived promoter in the AcMNPV baculovirus vector.

    abstract::The promoter sequences of the encoding genes for the three most abundant hexamerins of the Lepidoptera Trichoplusia ni were isolated and cloned into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-derived baculovirus expression vector. From the sequences analyzed, the DNA region driving the expressio...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.03.012

    authors: López-Vidal J,Gómez-Sebastián S,Sánchez-Ramos I,Escribano JM

    更新日期:2013-06-10 00:00:00

  • Antigen handling in the gastrointestinal lamina propria.

    abstract::Enteric infections associated with bacteria, viruses and parasites are a world health problem accounting for at least one billion episodes of diarrhoea and up to 10 million deaths in the Third World each year. The widely held conclusion that responses which are protective in the gastrointestinal tract are best stimula...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/0168-1656(95)00114-X

    authors: Stokes CR,Bailey M

    更新日期:1996-01-26 00:00:00

  • Dual nutrient limited growth: models, experimental observations, and applications.

    abstract::Dual nutrient limited growth, the control of the cell growth rate (kinetic aspect) or the restriction of the amount of biomass (stoichiometric aspect) by two nutrients at the same time, is a relatively unknown ability of the microorganisms and consequently, still not mentioned in textbooks to date. Nevertheless, multi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审

    doi:10.1016/j.jbiotec.2004.03.030

    authors: Zinn M,Witholt B,Egli T

    更新日期:2004-09-30 00:00:00

  • Microbial biomass production from rice straw hydrolysate in airlift bioreactors.

    abstract::Rice straw is a by-product of rice production, and a great bioresource as raw biomass material for manufacturing value-adding protein for animal feedstock, which has been paid more and more attention. In the present work, utilizing rice straw hydrolysate as a substrate for microbial biomass production in 11.5L externa...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2005.04.022

    authors: Zheng YG,Chen XL,Wang Z

    更新日期:2005-09-10 00:00:00

  • Lipase-catalyzed enantioselective synthesis of (R,R)-lactide from alkyl lactate to produce PDLA (poly D-lactic acid) and stereocomplex PLA (poly lactic acid).

    abstract::R-lactide, a pivotal monomer for the production of poly (D-lactic acid) (PDLA) or stereocomplex poly (lactic acid) (PLA) was synthesized from alkyl (R)-lactate through a lipase-catalyzed reaction without racemization. From among several types of lipase, only lipase B from Candida antarctica (Novozym 435; CAL-B) was ef...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2013.06.021

    authors: Jeon BW,Lee J,Kim HS,Cho DH,Lee H,Chang R,Kim YH

    更新日期:2013-10-20 00:00:00

  • Complete genome sequence of Bifidobacterium catenulatum JCM 1194(T) isolated from human feces.

    abstract::Bifidobacterium catenulatum JCM 1194(T) was isolated from human feces. This paper is the first report demonstrating the fully sequenced and completely annotated genome of a B. catenulatum strain. ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2015.06.415

    authors: Morita H,Toh H,Oshima K,Nakano A,Yamashita N,Iioka E,Arakawa K,Suda W,Honda K,Hattori M

    更新日期:2015-09-20 00:00:00

  • Complete genome sequence of the methanogenic neotype strain Methanobacterium formicicum MF(T.).

    abstract::The neotype strain Methanobacterium formicicum MF(T) (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074bp in size, featuring a GC content of 41.23%. M. fo...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2014.09.018

    authors: Maus I,Stantscheff R,Wibberg D,Stolze Y,Winkler A,Pühler A,König H,Schlüter A

    更新日期:2014-12-20 00:00:00

  • Studies on kinetic parameters and stability of aminoacylase in non-conventional media.

    abstract::Catalytic properties and conformational stability of aminoacylase (N-acylamino acid amidohydrolase, EC 3.5.1.14) were studied in water-N,N-dimethylformamide (DMF) and water-dioxane solvent mixtures. Beside the prompt inhibition the solvents caused further inactivation during incubations. In the presence of 5% DMF cont...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(98)00158-8

    authors: Boross L,Kosáry J,Stefanovits-Bányai E,Sisak C,Szajáni B

    更新日期:1998-11-18 00:00:00

  • Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus.

    abstract::Pellet growth of Aspergillus terreus ATCC 20542 in submerged batch fermentations in stirred bioreactors was used to examine the effects of agitation (impeller tip speed u(t) of 1.01-2.71 ms(-1)) and aeration regimens (air or an oxygen-enriched mixture containing 80% oxygen and 20% nitrogen by volume) on the fungal pel...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2004.10.005

    authors: Casas López JL,Sánchez Pérez JA,Fernández Sevilla JM,Rodríguez Porcel EM,Chisti Y

    更新日期:2005-03-02 00:00:00

  • Rewiring the reductive tricarboxylic acid pathway and L-malate transport pathway of Aspergillus oryzae for overproduction of L-malate.

    abstract::Aspergillus oryzae finds wide application in the food, feed, and wine industries, and is an excellent cell factory platform for production of organic acids. In this work, we achieved the overproduction of L-malate by rewiring the reductive tricarboxylic acid (rTCA) pathway and L-malate transport pathway of A. oryzae N...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2017.05.011

    authors: Liu J,Xie Z,Shin HD,Li J,Du G,Chen J,Liu L

    更新日期:2017-07-10 00:00:00

  • Characterization of stress and protein turnover from protein overexpression in fed-batch E. coli cultures.

    abstract::A structured kinetic model that accounts for proteolytic degradation due to recombinant protein overexpression is introduced and its performance evaluated by comparison with previously reported fed-batch experimental data. This mathematical model contains an additional pool for a generic key precursor (in our case phe...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/s0168-1656(99)00014-0

    authors: Ramírez DM,Bentley WE

    更新日期:1999-05-28 00:00:00

  • Microbiome response to controlled shifts in ammonium and LCFA levels in co-digestion systems.

    abstract::Anaerobic co-digestion using protein-rich and lipid-rich co-substrates is limited by the accumulation of ammonia and long chain fatty acids (LCFAs), which are important inhibitors of the anaerobic microorganisms. This work aimed to study the microbial community dynamics during gradual and abrupt increase in ammonium a...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2016.01.006

    authors: Regueiro L,Carballa M,Lema JM

    更新日期:2016-02-20 00:00:00

  • Preparation and characterization of folate-poly(ethylene glycol)-grafted-trimethylchitosan for intracellular transport of protein through folate receptor-mediated endocytosis.

    abstract::To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocy...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2009.09.007

    authors: Zheng Y,Song X,Darby M,Liang Y,He L,Cai Z,Chen Q,Bi Y,Yang X,Xu J,Li Y,Sun Y,Lee RJ,Hou S

    更新日期:2010-01-01 00:00:00

  • High level expression of proteins using sequences from the ferritin heavy chain gene locus.

    abstract::An expression vector has been generated using a gene highly expressed under conditions found in a typical fed-batch bioreactor process. The ferritin heavy chain (HC) gene exhibits higher levels of expression in the late stages of a fed-batch bioreactor than in the early stages. This property was considered advantageou...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2006.09.021

    authors: Prentice HL,Tonkin CJ,Caamano L,Sisk WP

    更新日期:2007-01-30 00:00:00

  • Stoichiometric and kinetic characterisation of Nitrosomonas sp. in mixed culture by decoupling the growth and energy generation processes.

    abstract::A novel method that relies on the decoupling of the energy production and biosynthesis processes was used to characterise the maintenance, cell lysis and growth processes of Nitrosomonas sp. A Nitrosomonas culture was enriched in a sequencing batch reactor (SBR) with ammonium as the sole energy source. Fluorescent in ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2006.04.017

    authors: Vadivelu VM,Keller J,Yuan Z

    更新日期:2006-11-10 00:00:00

  • Development of a high-throughput method to evaluate the impact of inhibitory compounds from lignocellulosic hydrolysates on the growth of Zymomonas mobilis.

    abstract::Overcoming the effects of hydrolysate toxicity towards ethanologens is a key technical barrier in the biochemical conversion process for biomass feedstocks to ethanol. Despite its importance, the complexity of the hydrolysate toxicity phenomena and the lack of systematic studies, analysis and tools surrounding this is...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章

    doi:10.1016/j.jbiotec.2009.08.006

    authors: Franden MA,Pienkos PT,Zhang M

    更新日期:2009-12-01 00:00:00