当前位置： SCI文献检索 > JOURNAL OF BIOTECHNOLOGY期刊下所有文献 > Rewiring the reductive tricarboxylic acid pathway and L-malate transport pathway of Aspergillus oryzae for overproduction of L-malate.

Rewiring the reductive tricarboxylic acid pathway and L-malate transport pathway of Aspergillus oryzae for overproduction of L-malate.

Abstract：

:Aspergillus oryzae finds wide application in the food, feed, and wine industries, and is an excellent cell factory platform for production of organic acids. In this work, we achieved the overproduction of L-malate by rewiring the reductive tricarboxylic acid (rTCA) pathway and L-malate transport pathway of A. oryzae NRRL 3488. First, overexpression of native pyruvate carboxylase and malate dehydrogenase in the rTCA pathway improved the L-malate titer from 26.1gL-1 to 42.3gL-1 in shake flask culture. Then, the oxaloacetate anaplerotic reaction was constructed by heterologous expression of phosphoenolpyruvate carboxykinase and phosphoenolpyruvate carboxylase from Escherichia coli, increasing the L-malate titer to 58.5gL-1. Next, the export of L-malate from the cytoplasm to the external medium was strengthened by overexpression of a C4-dicarboxylate transporter gene from A. oryzae and an L-malate permease gene from Schizosaccharomyces pombe, improving the L-malate titer from 58.5gL-1 to 89.5gL-1. Lastly, guided by transcription analysis of the expression profile of key genes related to L-malate synthesis, the 6-phosphofructokinase encoded by the pfk gene was identified as a potential limiting step for L-malate synthesis. Overexpression of pfk with the strong sodM promoter increased the L-malate titer to 93.2gL-1. The final engineered A. oryzae strain produced 165gL-1 L-malate with a productivity of 1.38gL-1h-1 in 3-L fed-batch culture. Overall, we constructed an efficient L-malate producer by rewiring the rTCA pathway and L-malate transport pathway of A. oryzae NRRL 3488, and the engineering strategy adopted here may be useful for the construction of A. oryzae cell factories to produce other organic acids.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Liu J,Xie Z,Shin HD,Li J,Du G,Chen J,Liu L

doi

10.1016/j.jbiotec.2017.05.011

subject

Has Abstract

pub_date

2017-07-10 00:00:00

pages

1-9

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(17)30242-0

journal_volume

253

pub_type

杂志文章

相关文献

JOURNAL OF BIOTECHNOLOGY文献大全
  • Mechanisms of ammonia and ammonium ion toxicity in animal cells: transport across cell membranes.

    abstract：:A model for transport of ammonia and ammonium ions across cell membranes is presented. The model suggests that ammonium ions compete with potassium ions for inward transport, over the cytoplasmic membrane, via potassium transport proteins like the Na+/K(+)-ATPase and the Na+K+2Cl(-)-cotransporter. It also explains the...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(93)90148-g

    authors： Martinelle K,Häggström L

    更新日期：1993-09-01 00:00:00

  • Continuous gas fermentation by Acetobacterium woodii in a submerged membrane reactor with full cell retention.

    abstract：:Acetogenic bacteria like Acetobacterium woodii represent an ancient group of anaerobic microorganisms which use hydrogen and carbon dioxide to produce acetate. Cell concentrations and space-time yields are usually low in gas fermentations. A standard stirred‑tank bioreactor with continuous gas supply was equipped with...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2015.07.020

    authors： Kantzow C,Mayer A,Weuster-Botz D

    更新日期：2015-10-20 00:00:00

  • Nontypeable Haemophilus influenzae: challenges in developing a vaccine.

    abstract：:Nontypeable Haemophilus influenzae (NTHi) is a gram-negative coccobacillus that is one of the bacteria that form the commensal flora of the upper respiratory tract in humans. This bacterium is an important human pathogen causing a broad spectrum of disease in both adults and children, including invasive and localised ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1656(99)00113-3

    authors： Kyd J,Cripps A

    更新日期：1999-08-20 00:00:00

  • Transfection of partially purified plasmid DNA for high level transient protein expression in HEK293-EBNA cells.

    abstract：:One of the major constraints to performing large-scale transfections of cultured mammalian cells for the transient expression of recombinant proteins is the production of large quantities of purified plasmid DNA. In this report partially purified plasmid DNA was prepared by a method that combines alkaline lysis of E. ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(03)00032-4

    authors： Wright JL,Jordan M,Wurm FM

    更新日期：2003-05-08 00:00:00

  • Metabolic flux analysis using stoichiometric models for Aspergillus niger: comparison under glucoamylase-producing and non-producing conditions.

    abstract：:Aspergillus niger AB1.13 cultures with glucoamylase production (with D-glucose as substrate) and without glucoamylase production (with D-xylose as substrate) were characterized by metabolic flux analysis. Two comprehensive metabolic models for d-glucose- as well as for D-xylose-consumption were used to quantify and co...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.08.034

    authors： Melzer G,Dalpiaz A,Grote A,Kucklick M,Göcke Y,Jonas R,Dersch P,Franco-Lara E,Nörtemann B,Hempel DC

    更新日期：2007-12-01 00:00:00

  • Transgenic rabbits as bioreactors for the production of human growth hormone.

    abstract：:Gene farming is one of the most promising areas in modern biotechnology. To assay the potential usefulness of transgenic rabbits as bioreactors, one call embryos were microinjected with a chimeric gene comprising 5' sequences from mouse whey acidic protein gene (mWAP) linked to the human growth hormone (hGH) gene. Tra...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(95)00026-m

    authors： Limonta JM,Castro FO,Martínez R,Puentes P,Ramos B,Aguilar A,Lleonart RL,de la Fuente J

    更新日期：1995-05-15 00:00:00

  • The use of surface plasmon resonance (SPR) and fluorescence resonance energy transfer (FRET) to monitor the interaction of the plant G-proteins Ms-Rac1 and Ms-Rac4 with GTP.

    abstract：:Using an RT-PCR approach a cDNA clone, designated Ms-Rac4 and putatively coding for a small GTPase was isolated from Medicago sativa. Ms-Rac4 and the earlier described Ms-Rac1 [Mol. Gen. Genet. 263 (2000) 761] belong to the class of GTP-binding Rho of plants (Rop) proteins. At the amino acid level they display all con...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2004.04.030

    authors： Brecht M,Sewald K,Schiene K,Keen G,Fricke M,Sauer M,Niehaus K

    更新日期：2004-08-26 00:00:00

  • The heparanome--the enigma of encoding and decoding heparan sulfate sulfation.

    abstract：:Heparan sulfate (HS) is a cell surface carbohydrate polymer modified with sulfate moieties whose highly ordered composition is central to directing specific cell signaling events. The ability of the cell to generate these information rich glycans with such specificity has opened up a new field of "heparanomics" which ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jbiotec.2007.01.022

    authors： Lamanna WC,Kalus I,Padva M,Baldwin RJ,Merry CL,Dierks T

    更新日期：2007-04-30 00:00:00

  • Long-term stability of marker gene expression in Prunus subhirtella: a model fruit tree species.

    abstract：:Transgenic trees currently are being produced by Agrobacterium-mediated transformation and biolistics. Since trees are particularly suited for long-term evaluations of the impact of the technology, Prunus subhirtella autumno rosa (PAR) was chosen as model fruit tree species and transformed with a reporter gene (uidA) ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2006.06.016

    authors： Maghuly F,da Câmara Machado A,Leopold S,Khan MA,Katinger H,Laimer M

    更新日期：2007-01-01 00:00:00

  • The complete genome sequence of a lactic acid bacterium Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484(T).

    abstract：:Leuconostoc species are widespread in the natural environment and play an important role in several types of industrial and food fermentation processes. Here, we report the 1,854,727-bp complete genome sequence of the Leuconostoc mesenteroides ssp. dextranicum strain DSM 20484(T). ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2015.12.009

    authors： Park GS,Hong SJ,Jung BK,Lee C,Park CK,Shin JH

    更新日期：2016-02-10 00:00:00

  • Measurement of volumetric (OUR) and determination of specific (qO2) oxygen uptake rates in animal cell cultures.

    abstract：:Oxygen is a key substrate in animal cell metabolism. It has been reported that the oxygen uptake rate (OUR) is a good indicator of cellular activity, and even under some conditions, a good indicator of the number of viable cells. The measurement of OUR is difficult due to many different reasons. In particular, the ver...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章,评审

    doi：10.1016/s0168-1656(98)00046-7

    authors： Ruffieux PA,von Stockar U,Marison IW

    更新日期：1998-08-12 00:00:00

  • Synthesis of a novel macrolactone by lipase-catalyzed intra-esterification of hydroxy-fatty acid in organic media.

    abstract：:The unsaturations and groups bound to the ring and to the lateral chain of lactones give a large diversity in this class of molecules. In this work we produced enzymatically a macrolactone in organic media. The substrate used was a hydroxy-fatty acid: (+)-coriolic acid and the enzymes tested were free or immobilized m...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/s0168-1656(01)00374-1

    authors： Gargouri M,Drouet P,Legoy MD

    更新日期：2002-01-18 00:00:00

  • Analysis of polymer molecular weight distributions in aqueous two-phase systems.

    abstract：:The partitioning of proteins and other biomaterials between two aqueous phases containing polyethyleneglycol and dextran is a strong function of the molecular weight of the two polymers. Although both polymers are polydispersed (especially Dx) most theoretical treatments refer only to the average molecular weight (num...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(91)90224-j

    authors： Forciniti D,Hall CK,Kula MR

    更新日期：1991-09-01 00:00:00

  • Genome improvement of the acarbose producer Actinoplanes sp. SE50/110 and annotation refinement based on RNA-seq analysis.

    abstract：:Actinoplanes sp. SE50/110 is the natural producer of acarbose, which is used in the treatment of diabetes mellitus type II. However, until now the transcriptional organization and regulation of the acarbose biosynthesis are only understood rudimentarily. The genome sequence of Actinoplanes sp. SE50/110 was known befor...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2017.04.013

    authors： Wolf T,Schneiker-Bekel S,Neshat A,Ortseifen V,Wibberg D,Zemke T,Pühler A,Kalinowski J

    更新日期：2017-06-10 00:00:00

  • Enzymatic peptide synthesis in organic media: a comparative study of water-miscible and water-immiscible solvent systems.

    abstract：:Peptide synthesis was carried out in a variety of organic solvents with low contents of water. The enzyme was deposited on the support material, celite, from an aqueous buffer solution. After evaporation of the water the biocatalyst was suspended in the reaction mixtures. The chymotrypsin-catalyzed reaction between Z-...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/0168-1656(90)90090-x

    authors： Clapés P,Adlercreutz P,Mattiasson B

    更新日期：1990-09-01 00:00:00

  • Expression of the porcine circovirus type 2 capsid protein subunits and application to an indirect ELISA.

    abstract：:Porcine circovirus type 2 (PCV2) is considered to be associated with post-weaning multisystemic wasting syndrome (PMWS), which is a newly emerged economically important swine disease. The entire coding region of open reading frame 2 (ORF2), encoding the viral capsid protein (Cap), of PCV2 was cloned and sequenced from...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.09.015

    authors： Wu PC,Chien MS,Tseng YY,Lin J,Lin WL,Yang CY,Huang C

    更新日期：2008-01-01 00:00:00

  • Stoichiometric and kinetic characterisation of Nitrosomonas sp. in mixed culture by decoupling the growth and energy generation processes.

    abstract：:A novel method that relies on the decoupling of the energy production and biosynthesis processes was used to characterise the maintenance, cell lysis and growth processes of Nitrosomonas sp. A Nitrosomonas culture was enriched in a sequencing batch reactor (SBR) with ammonium as the sole energy source. Fluorescent in ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2006.04.017

    authors： Vadivelu VM,Keller J,Yuan Z

    更新日期：2006-11-10 00:00:00

  • Gene expression analysis using a modified HiCEP method applicable to prokaryotes: a study of the response of Rhodococcus to isoniazid and ethambutol.

    abstract：:We established a novel method to analyze the gene expression of prokaryotes by modifying and optimizing the HiCEP (high coverage gene expression analysis) method, which was originally developed for eukaryotic gene expression profiling. Following the removal of abundant rRNA, the mRNA of prokaryotic cells was enzymatic...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2005.11.004

    authors： Mitani Y,Suzuki K,Kondo K,Okumura K,Tamura T

    更新日期：2006-05-29 00:00:00

  • A new arylesterase from Pseudomonas pseudoalcaligenes can hydrolyze ionic phthalic polyesters.

    abstract：:Extracellular enzymes are assumed to be responsible for the initial and rate limiting step in biodegradation of polymers. Mainly enzymes with aliphatic esters as their natural substrates (e.g. lipase, cutinases) have until now been evaluated for polyester hydrolysis studies. However, the potential of enzymes with arom...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2017.01.012

    authors： Haernvall K,Zitzenbacher S,Yamamoto M,Schick MB,Ribitsch D,Guebitz GM

    更新日期：2017-09-10 00:00:00

  • Development of targeted gene transfer into human primary T lymphocytes and macrophages using high-titer recombinant HIV vectors.

    abstract：:Primary human lymphocytes and macrophages are an important target cells for human immunodeficiency virus (HIV). For targeted gene transfer into CD4(+) lymphocytes and macrophages, we constructed HIV vectors with envelope glycoprotein (gp120) from the T-cell tropic BH10 strain and the macrophage tropic SF162, and devel...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2007.01.004

    authors： Miyake K,Miyake N,Shimada T

    更新日期：2007-05-01 00:00:00

  • Additional virulence genes in conjunction with efficient selection scheme, and compatible culture regime enhance recovery of stable transgenic plants in cowpea via Agrobacterium tumefaciens-mediated transformation.

    abstract：:A critical step in the development of robust Agrobacterium tumefaciens-mediated transformation system in recalcitrant grain legume, cowpea is the establishment of optimal conditions for efficient T-DNA delivery into target tissue and recovery of transgenic plants. A dramatic increase in efficiency of T-DNA delivery wa...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2008.02.008

    authors： Solleti SK,Bakshi S,Sahoo L

    更新日期：2008-05-20 00:00:00

  • Engineered Salmonella allows real-time heterologous gene expression monitoring within infected zebrafish embryos.

    abstract：:Microbial host-pathogen interactions have been traditionally well studied at genetic and physiological levels, but cell-resolution analyses have been particularly scarce. This has been especially remarkable for intracellular parasites for two major reasons: first, the inherent loss of bacteria traceability once infect...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2011.11.022

    authors： Medina C,Santero E,Gómez-Skarmeta JL,Royo JL

    更新日期：2012-02-10 00:00:00

  • Morphologically-structured models of growing budding yeast populations.

    abstract：:It has been well recognized that many key aspects of cell cycle regulation are encoded into the size distributions of growing budding yeast populations due to the tight coupling between cell growth and cell division present in this organism. Several attempts have been made to model the cell size distribution of growin...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2006.01.011

    authors： Hatzis C,Porro D

    更新日期：2006-07-13 00:00:00

  • In vitro co-culture model of medulloblastoma and human neural stem cells for drug delivery assessment.

    abstract：:Physiologically relevant in vitro models can serve as biological analytical platforms for testing novel treatments and drug delivery systems. We describe the first steps in the development of a 3D human brain tumour co-culture model that includes the interplay between normal and tumour tissue along with nutrient gradi...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2015.01.002

    authors： Ivanov DP,Parker TL,Walker DA,Alexander C,Ashford MB,Gellert PR,Garnett MC

    更新日期：2015-07-10 00:00:00

  • Finding edging genes from microarray data.

    abstract：MOTIVATION:A set of genes and their gene expression levels are used to classify disease and normal tissues. Due to the massive number of genes in microarray, there are a large number of edges to divide different classes of genes in microarray space. The edging genes (EGs) can be co-regulated genes, they can also be on ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2008.04.004

    authors： An J,Chen YP

    更新日期：2008-06-30 00:00:00

  • High-throughput fluorescence screening assay for the identification and comparison of antimicrobial peptides' activity on various yeast species.

    abstract：:New antifungal compounds that circumvent the resistance of the pathogen by directly damaging yeast cell surface structures are promising agents for the treatment of fungal infections, due to their different mechanism of action from current clinically used antifungal drugs. We present here a rapid and cost-effective fl...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2016.06.023

    authors： Kodedová M,Sychrová H

    更新日期：2016-09-10 00:00:00

  • Phage display allows identification of zona pellucida-binding peptides with species-specific properties: novel approach for development of contraceptive vaccines for wildlife.

    abstract：:Multiple phage-peptide constructs, where the peptides mimic sperm epitopes that bind to zona pellucida (ZP) proteins, were generated via selection from a phage display library using a novel approach. Selections were designed to allow for identification of ZP-binding phage clones with potential species-specific propert...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2012.10.006

    authors： Samoylova TI,Cochran AM,Samoylov AM,Schemera B,Breiteneicher AH,Ditchkoff SS,Petrenko VA,Cox NR

    更新日期：2012-12-31 00:00:00

  • Immobilization of Aspergillus terreus lipase in self-assembled hollow nanospheres for enantioselective hydrolysis of ketoprofen vinyl ester.

    abstract：:The aim of this study was to improve the ability of Aspergillus terreus lipase to separate the racemic ketoprofen vinyl ester into individual enantiomers using hollow self-assembly alginate-graft-poly(ethylene glycol)/α-cyclodextrins (Alg-g-PEG/α-CD) spheres as enzyme immobilization carriers. The morphology and size o...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2014.11.032

    authors： Hu C,Wang N,Zhang W,Zhang S,Meng Y,Yu X

    更新日期：2015-01-20 00:00:00

  • Vibrio cholerae periplasmic superoxide dismutase: isolation of the gene and overexpression of the protein.

    abstract：:Superoxide dismutases are ubiquitous enzymes which play an important role in protecting cells against oxidative damage and which have also been shown to contribute to the pathogenicity of many bacterial species. Here we demonstrate that Vibrio cholerae, the causative agent of cholerae, expresses an active periplasmic ...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2004.01.002

    authors： Gabbianelli R,Signoretti C,Marta I,Battistoni A,Nicolini L

    更新日期：2004-04-08 00:00:00

  • Polyhydroxyalkanoate (PHA) synthase genes and PHA-associated gene clusters in Pseudomonas spp. and Janthinobacterium spp. isolated from Antarctica.

    abstract：:The polyhydroxyalkanoate (PHA) producing capability of four bacterial strains isolated from Antarctica was reported in a previous study. This study analyzed the PHA synthase genes and the PHA-associated gene clusters from the two antarctic Pseudomonas isolates (UMAB-08 and UMAB-40) and the two antarctic Janthinobacter...

    journal_title：Journal of biotechnology

    pub_type： 杂志文章

    doi：10.1016/j.jbiotec.2020.03.006

    authors： Tan IKP,Foong CP,Tan HT,Lim H,Zain NA,Tan YC,Hoh CC,Sudesh K

    更新日期：2020-04-10 00:00:00