Abstract：
:A new current amplified immunosensor for the determination of carcinoembryonic antigen (CEA) was demonstrated in this work. The electrode architecture was fabricated by positively charged toluidine blue (TB) coated on negatively charged poly-sulfanilic acid (PSAA) modified glassy carbon electrode (GCE) surface through electrostatic interactions to form a TB/PSAA film, which provided an interface containing amine groups to assemble gold nanoparticles (nano-Au) for immobilization of carcinoembryonic antibody (anti-CEA) and horseradish peroxidase (HRP) instead of bovine serum albumin (BSA) to block sites against non-specific binding. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were employed to characterize the electrochemical properties of the modified processes. The CVs reduction current of the immunosensor charged linearly in two concentration ranges of CEA from 0.5 to 5.0 and 5.0 to 120.0 ng/ml in presence of 0.3mM H2O2 in analyte solution, and the detection limit was 0.2 ng/ml at three times background noise. The proposed method is economical, efficient and potentially attractive for clinical immunoassays.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Li X,Yuan R,Chai Y,Zhang L,Zhuo Y,Zhang Ydoi
10.1016/j.jbiotec.2005.11.023subject
Has Abstractpub_date
2006-05-29 00:00:00pages
356-66issue
3eissn
0168-1656issn
1873-4863pii
S0168-1656(06)00006-Xjournal_volume
123pub_type
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