Refolding of hexameric porcine leucine aminopeptidase using a cationic detergent and dextrin-10 as artificial chaperones.

Abstract:

:The application of artificial chaperones in biotechnology has been inspired by the mechanism of molecular chaperones like GroEL/GroES. It involves addition of a capturing detergent during dilution of the chaotropic reagent, that prevents protein aggregation, and finally, addition of a oligosaccharide that removes the detergent allowing the protein to refold. Here, guanidinium hydrochloride-denatured hexameric leucine aminopeptidase is shown to be efficiently refolded by using the cationic detergent cetyltrimethylammonium bromide and the linear polysaccharide dextrin-10 as artificial chaperones. The effect of these additives and the time dependence on the recovery of total enzymatic activity, kinetic parameters (K(M), k(cat)), intrinsic steady-state tryptophan fluorescence and oligomeric structure is presented. The method described is very promising since 92% of fully active and correct folded LAP could be produced. Moreover, we showed that the stripping process is relatively slow, it allows the protein to refold almost entirely to its native state.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Laslo AC,Ganea E,Obinger C

doi

10.1016/j.jbiotec.2009.01.023

subject

Has Abstract

pub_date

2009-03-25 00:00:00

pages

162-8

issue

3-4

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(09)00040-6

journal_volume

140

pub_type

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