Production of ω-hydroxy octanoic acid with Escherichia coli.

abstract：:Expanded bed adsorption chromatography is used to capture the protein product of interest from a crude biological suspension directly, thereby eliminating the need for the removal of the cell debris. While this technique may replace three or four unit operations in a typical downstream process for biological product r...

journal_title：Journal of biotechnology

authors： Balasundaram B,Harrison ST

更新日期：2008-02-01 00:00:00

abstract：:The transfer of genetic information between distantly or even unrelated organisms during evolution had been inferred from nucleotide sequence comparisons. These studies provided circumstantial evidence that in rare cases genes had been laterally transmitted amongst organisms of the domains bacteria, archaea and eukary...

journal_title：Journal of biotechnology

authors： Dröge M,Pühler A,Selbitschka W

更新日期：1998-09-17 00:00:00

abstract：:Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with biotic and abiotic elicitors to evaluate their effect on secondary metabolism. Metabolic profiles analysed by (1)H NMR spectroscopy and principal component analysis (PCA) showed variations in some of th...

journal_title：Journal of biotechnology

authors： Flores-Sanchez IJ,Pec J,Fei J,Choi YH,Dusek J,Verpoorte R

更新日期：2009-08-20 00:00:00

abstract：:In vitro display technologies, such as mRNA display and DNA display are powerful tools to screen peptides and proteins with desired functions from combinatorial libraries in the fields of directed protein evolution and proteomics. When screening combinatorial libraries of polypeptides (phenotype), each of which is dis...

journal_title：Journal of biotechnology

authors： Doi N,Takashima H,Wada A,Oishi Y,Nagano T,Yanagawa H

更新日期：2007-09-15 00:00:00

abstract：:Magnetic Fe3O4 nanoparticles were prepared and embedded into the Combi-CLEAs to produce the magnetic Combi-CLEAs in this work. The process for magnetic Combi-CLEAs preparation was optimized, and its properties were investigated. The optimum temperature, thermal stability and optimum pH of magnetic Combi-CLEAs were sim...

journal_title：Journal of biotechnology

authors： Su E,Meng Y,Ning C,Ma X,Deng S

更新日期：2018-04-10 00:00:00

abstract：:A novel bacterial species was isolated from an industrial-scale biogas plant. The isolate Herbinix hemicellulosilytica T3/55(T) is able to degrade crystalline cellulose. Comparative 16S rRNA gene sequencing demonstrated that the isolate is closely related to environmental samples forming a hitherto unknown sub-cluster...

journal_title：Journal of biotechnology

authors： Koeck DE,Maus I,Wibberg D,Winkler A,Zverlov VV,Liebl W,Pühler A,Schwarz WH,Schlüter A

更新日期：2015-11-20 00:00:00

abstract：:Nanoliposomes containing phosphatidic acid or cardiolipin are able to target in vitro with very high affinity amyloid-β (Aβ), a peptide whose overproduction and progressive aggregation in the brain play a central role in the pathogenesis of Alzheimer's disease. However, the presence of the blood-brain barrier (BBB) se...

journal_title：Journal of biotechnology

authors： Re F,Cambianica I,Sesana S,Salvati E,Cagnotto A,Salmona M,Couraud PO,Moghimi SM,Masserini M,Sancini G

更新日期：2011-12-20 00:00:00

abstract：:In the important industrial yeast Saccharomyces cerevisiae, galactose metabolism requires energy production by respiration; therefore, this yeast cannot metabolize galactose under strict anaerobic conditions. While the respiratory dependence of galactose metabolism provides benefits in terms of cell growth and populat...

journal_title：Journal of biotechnology

authors： Quarterman J,Skerker JM,Feng X,Liu IY,Zhao H,Arkin AP,Jin YS

更新日期：2016-07-10 00:00:00

abstract：:Combinatorial library selections through the systematic evolution of ligands by exponential enrichment (SELEX) technique identify so-called nucleic acid aptamers that bind with high-affinity and specificity to a wide range of selected molecules. However, the modest chemical functionality of nucleic acids poses some li...

journal_title：Journal of biotechnology

authors： Kusser W

更新日期：2000-03-01 00:00:00

abstract：:Secreted recombinant proteins can aggregate during cell culture. We studied a poorly-behaved bispecific scaffold that increasingly aggregated (up to 62% high molecular weight species, HMW) as a function of culture time in a fed-batch and intensified cell culture processes. We identified that protein aggregates increas...

journal_title：Journal of biotechnology

authors： Gomez N,Barkhordarian H,Lull J,Huh J,GhattyVenkataKrishna P,Zhang X

更新日期：2019-10-10 00:00:00

abstract：:The addition of 5-40 mM L-glutamine to batch cultures of a murine hybridoma following the cessation of rapid growth significantly stimulated monoclonal antibody (mAb) synthesis and secretion per cell. Stimulation of mAb secretion following the cessation of rapid growth was also observed in response to addition of mito...

journal_title：Journal of biotechnology

authors： Flickinger MC,Goebel NK,Bibila T,Boyce-Jacino S

更新日期：1992-02-01 00:00:00

abstract：:Group I grass pollen allergens are major contributors to grass pollen-related seasonal allergic rhinitis, and as such a primary target for allergen specific immunotherapy. In this study the potential therapeutic role of oral application of Lactobacillus plantarum WCFS1, directing cell wall attachment of the recombinan...

journal_title：Journal of biotechnology

authors： Minic R,Gavrovic-Jankulovic M,Petrusic V,Zivkovic I,Eijsink VG,Dimitrijevic L,Mathiesen G

更新日期：2015-04-10 00:00:00

abstract：:To overcome the low efficiency of agronomic protection from maize dwarf mosaic disease, susceptible maize inbred line was transformed by Agrobacterium tumefaciens harboring hpRNA expression vectors containing inverted-repeat sequences of different lengths targeting coat protein gene (CP) of maize dwarf mosaic virus (M...

journal_title：Journal of biotechnology

authors： Zhang ZY,Yang L,Zhou SF,Wang HG,Li WC,Fu FL

更新日期：2011-05-20 00:00:00

abstract：:Mining sequence data is increasingly important for biocatalysis research. However, when relying on sequence data alone, prediction of the reaction catalyzed by a specific protein sequence is often elusive, and substrate specificity is far from trivial. The present study demonstrated an approach of combining sequence d...

journal_title：Journal of biotechnology

authors： Seffernick JL,Samanta SK,Louie TM,Wackett LP,Subramanian M

更新日期：2009-08-10 00:00:00

abstract：:Continuous photoproduction of H(2) by the green alga, Chlamydomonas reinhardtii, is observed after incubating the cultures for about a day in the absence of sulfate and in the presence of acetate. Sulfur deprivation causes the partial and reversible inactivation of photosynthetic O(2) evolution in algae, resulting in ...

journal_title：Journal of biotechnology

authors： Kosourov S,Patrusheva E,Ghirardi ML,Seibert M,Tsygankov A

更新日期：2007-03-10 00:00:00

abstract：:The enzymatic bioconversion of chitin is of considerable interest for the natural production of bioactive compounds such as chitooligosaccharides and N-acetyl-d-glucosamine (GlcNAc). Key enzymes are involved in the natural processing of chitin, hydrolyzing this abundant biopolymer to yield chitooligosaccharides with s...

journal_title：Journal of biotechnology

authors： Nguyen-Thi N,Doucet N

更新日期：2016-02-20 00:00:00

abstract：:Hydrogen production by the dark fermentation of food wastes is an economic and environmentally friendly technology to produce the clean energy source as well as to treat the problematic wastes. However, the long-term operations of the continuous anaerobic reactor for fermentative hydrogen production were frequently un...

journal_title：Journal of biotechnology

authors： Jo JH,Jeon CO,Lee DS,Park JM

更新日期：2007-09-15 00:00:00

abstract：:The feed profile of glucose during fedbatch cultivation could be used to influence the retention of the periplasmic product ZZ-cutinase. An increased feed rate led to a higher production rate but also to an increased specific leakage, which reduced the periplasmic retention. Three growth rates: 0.3, 0.2 and 0.1 h(-1) ...

journal_title：Journal of biotechnology

authors： Bäcklund E,Reeks D,Markland K,Weir N,Bowering L,Larsson G

更新日期：2008-07-31 00:00:00

abstract：:The adsorption behaviors of two glucanases, TvEG and TrCel7A, on three lignins were investigated. Three lignins were isolated from raw aspen and its pretreated solid residue. The isolated lignins were labeled as Asp-MWL, DA-MWL (pretreated by dilute acid), and GL-MWL (pretreated by green liquor), respectively. The sur...

journal_title：Journal of biotechnology

authors： Zhang Y,Jiang X,Wan S,Wu W,Wu S,Jin Y

更新日期：2020-11-10 00:00:00

abstract：:A new, chemically defined medium providing dispersed growth and high biomass formation and a method for quantitative extraction of intracellular metabolites was used to investigate the cellular response of Streptomyces coelicolor A3(2) during growth and upon changes in nutrient utilization. Fast changes of the glucose...

journal_title：Journal of biotechnology

authors： Mira de Orduña R,Theobald U

更新日期：2000-02-17 00:00:00

abstract：:Aptamers are single-stranded DNA or RNA oligonucleotides, which have been emerging as recognition elements in disease diagnostics and food control, including the detection of bacterial toxins. In this study, we employed the semi-automated just in time-selection to identify aptamers that bind to cholera toxin (CT) with...

journal_title：Journal of biotechnology

authors： Frohnmeyer E,Frisch F,Falke S,Betzel C,Fischer M

更新日期：2018-03-10 00:00:00

abstract：:The stability, copy number, and gene expression of the pBR322 plasmid containing the par-locus under prolonged cultivation were studied. In the initial stage of the experiment it was observed that the par-locus had a stabilization effect on plasmid maintenance. This observation was consistent with previously reported ...

journal_title：Journal of biotechnology

authors： Weber AE,Yu P,San KY

更新日期：1991-04-01 00:00:00

abstract：:Roseateles depolymerans is a Gram-negative, rod-shaped, flagellated, obligately aerobic, photosynthetic bacterium that was isolated from the Hanamuro River, Ibaraki Prefecture, Japan. Here, we report the complete genome of R. depolymerans KCTC 42856(T) (=61A(T)=DSM 11813(T)=CCUG 48747(T)=NCIMB 13588(T)), which consist...

journal_title：Journal of biotechnology

authors： Lee SH,Choe H,Kim SG,Park DS,Nasir A,Kim BK,Kim KM

更新日期：2016-02-20 00:00:00

abstract：:Planococcus rifietoensis M8(T) (=DSM 15069(T)=ATCC BAA-790(T)) is a halotolerant bacterium with potential plant growth promoting properties isolated from an algal mat collected from a sulfurous spring in Campania (Italy). This paper presents the first complete genome of P. rifietoensis M8(T). Genes coding for various ...

journal_title：Journal of biotechnology

authors： See-Too WS,Convey P,Pearce DA,Lim YL,Ee R,Yin WF,Chan KG

更新日期：2016-03-10 00:00:00

abstract：:The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine ...

journal_title：Journal of biotechnology

authors： Capiaumont J,Legrand C,Carbonell D,Dousset B,Belleville F,Nabet P

更新日期：1995-02-21 00:00:00

abstract：:Chlorella vulgaris was grown in two bench-scale photobioreactors with and without the application of a low intensity, low frequency electromagnetic field (EM-ELF) of about 3mT. Cell concentration and tendency of cells to form aggregates inside the reactor were recorded over a 30 days-time period at 0.5L-constant mediu...

journal_title：Journal of biotechnology

authors： Beruto DT,Lagazzo A,Frumento D,Converti A

更新日期：2014-01-01 00:00:00

abstract：:2'-Fucosyllactose (2-FL) is one of most abundant functional oligosaccharides in human milk, which is involved in many biological functions for human health. To date, most microbial systems for 2-FL production have been limited to use Escherichia coli JM strains since they cannot metabolize lactose. In this study, E. c...

journal_title：Journal of biotechnology

authors： Chin YW,Kim JY,Lee WH,Seo JH

更新日期：2015-09-20 00:00:00

abstract：:Two cyanobacterial strains, Synechocystis sp. PCC 6803 and Nostoc punctiforme ATCC 29133 were immobilized within magnesium phosphate based cements, showing a viability and activity for at least 4 weeks. These biohybrids are considered as an alternative photobioreactor material for bioremediation or an improved yield o...

journal_title：Journal of biotechnology

authors： Vorndran E,Lindberg P

更新日期：2016-04-10 00:00:00

abstract：:Two-thirds of Earth's surface is covered by oceans, yet the study of this massive integrated living system is still in its infancy. Various environmental variables, such as high salinity, low and changeable nutrient availability and depth-correlated gradients of light, temperature, nutrients and pressure shape the div...

journal_title：Journal of biotechnology

authors： Simonato F,Campanaro S,Lauro FM,Vezzi A,D'Angelo M,Vitulo N,Valle G,Bartlett DH

更新日期：2006-10-20 00:00:00

abstract：:Escherichia coli phytase was covalently immobilized on NHS-activated Sepharose High Performance. The pH dependence of the phytase activity was not influenced by immobilization, whereas stability against heat treatment was enhanced as a consequence of immobilization. Compared to the free phytase the immobilized enzyme ...

journal_title：Journal of biotechnology

authors： Greiner R,Konietzny U

更新日期：1996-07-18 00:00:00