Abstract：
:Myxobacteria are well established as proficient producers of natural products with numerous biological activities. Although some knowledge has been gained regarding the biosynthesis of secondary metabolites in this class of bacteria, almost nothing is known about the underlying regulatory mechanisms. In order to identify regulatory elements, we submitted the argyrin and stigmatellin producer Cystobacter fuscus to a random transposon mutagenesis strategy and screened 1,000 mutants for the occurrence of strains showing remarkably increased or decreased production of these compounds. In addition to the identification of the stigmatellin biosynthetic gene cluster, a novel positive regulator (stiR) of stigmatellin production was identified after transposon recovery. In order to exclude secondary mutagenesis effects, a double cross-over mutagenesis strategy was applied to the strain resulting in the repeated generation of the transposon genotype. This strain was shown to be equally effected in natural product biosynthesis.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Rachid S,Sasse F,Beyer S,Müller Rdoi
10.1016/j.jbiotec.2005.08.014subject
Has Abstractpub_date
2006-02-24 00:00:00pages
429-41issue
4eissn
0168-1656issn
1873-4863pii
S0168-1656(05)00538-9journal_volume
121pub_type
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