Differentiation of four Aspergillus species and one Zygosaccharomyces with two electronic tongues based on different measurement techniques.

Abstract:

:Two electronic tongues based on different measurement techniques were applied to the discrimination of four molds and one yeast. Chosen microorganisms were different species of Aspergillus and yeast specie Zygosaccharomyces bailii, which are known as food contaminants. The electronic tongue developed in Linköping University was based on voltammetry. Four working electrodes made of noble metals were used in a standard three-electrode configuration in this case. The St. Petersburg electronic tongue consisted of 27 potentiometric chemical sensors with enhanced cross-sensitivity. Sensors with chalcogenide glass and plasticized PVC membranes were used. Two sets of samples were measured using both electronic tongues. Firstly, broths were measured in which either one of the molds or the yeast grew until late logarithmic phase or border of the stationary phase. Broths inoculated by either one of molds or the yeast was measured at five different times during microorganism growth. Data were evaluated using principal component analysis (PCA), partial least square regression (PLS) and linear discriminant analysis (LDA). It was found that both measurement techniques could differentiate between fungi species. Merged data from both electronic tongues improved differentiation of the samples in selected cases.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Söderström C,Rudnitskaya A,Legin A,Krantz-Rülcker C

doi

10.1016/j.jbiotec.2005.04.017

subject

Has Abstract

pub_date

2005-09-29 00:00:00

pages

300-8

issue

3

eissn

0168-1656

issn

1873-4863

pii

S0168-1656(05)00189-6

journal_volume

119

pub_type

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