Rapid degradation of ferulic acid via 4-vinylguaiacol and vanillin by a newly isolated strain of bacillus coagulans.


:A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was further demethylated to protocatechuic acid. Above 95% substrate degradation was obtained within 7 h of growth on ferulic acid medium, which is the shortest period of time reported to date. The major degradation products, was isolated and identified by thin-layer chromatography, high performance liquid chromatography and 1H-nuclear magnetic resonance spectroscopy were 4-vinylguaiacol, vanillin, vanillic acid and protocatechuic acid.


J Biotechnol


Journal of biotechnology


Karmakar B,Vohra RM,Nandanwar H,Sharma P,Gupta KG,Sobti RC




Has Abstract


2000-07-14 00:00:00














  • Lrp of Corynebacterium glutamicum controls expression of the brnFE operon encoding the export system for L-methionine and branched-chain amino acids.

    abstract::Corynebacterium glutamicum possesses export systems for various amino acids including BrnFE, a two-component export system for L-methionine and the branched-chain amino acids L-valine, L-isoleucine and L-leucine. A gene for a putative transcriptional regulator of the Lrp family is transcribed divergently to the brnFE ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lange C,Mustafi N,Frunzke J,Kennerknecht N,Wessel M,Bott M,Wendisch VF

    更新日期:2012-04-30 00:00:00

  • Enhanced porcine circovirus Cap protein production by Pichia pastoris with a fuzzy logic DO control based methanol/sorbitol co-feeding induction strategy.

    abstract::Porcine circovirus Cap protein production by P. pastoris with strong AOX promoter suffered with the problems with traditional pure methanol induction: (1) inefficient methanol metabolism; (2) extensive oxygen supply load; (3) difficulty in stable DO control; (4) low protein titer. In this study, based on the differenc...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ding J,Zhang C,Gao M,Hou G,Liang K,Li C,Ni J,Li Z,Shi Z

    更新日期:2014-05-10 00:00:00

  • Biodegradation of benzene, toluene, ethylbenzene, and o-xylene by a coculture of Pseudomonas putida and Pseudomonas fluorescens immobilized in a fibrous-bed bioreactor.

    abstract::A fibrous-bed bioreactor containing the coculture of Pseudomonas putida and P. fluorescens immobilized in a fibrous matrix was developed to degrade benzene (B), toluene (T), ethylbenzene (E), and o-xylene (X) in synthetic waste streams. The kinetics of BTEX biodegradation by immobilized cells adapted in the fibrous-be...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Shim H,Yang ST

    更新日期:1999-01-22 00:00:00

  • A novel short chain dehydrogenase from Bacillus megaterium for the conversion of the sesquiterpene nootkatol to (+)-nootkatone.

    abstract::(+)-Nootkatone is a natural ingredient that occurs in grapefruit and certain other plants and is responsible for the characteristic smell of grapefruit. Due to its versatile applications in the flavor and fragrance industry as well as its application in some medical uses it recruits the interests of academic research ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Milhim M,Hartz P,Gerber A,Bernhardt R

    更新日期:2019-08-10 00:00:00

  • Hydrophobicity engineering to facilitate surface display of heterologous gene products on Staphylococcus xylosus.

    abstract::Protein engineering has been employed to investigate the effect of specific amino acid changes on the targeting of heterologous proteins to the outer cell surface of the Gram-positive bacterium Staphylococcus xylosus. Three different variants, corresponding to a 101 amino acid region of the major glycoprotein (G prote...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Nguyen TN,Gourdon MH,Hansson M,Robert A,Samuelson P,Libon C,Andréoni C,Nygren PA,Binz H,Uhlén M

    更新日期:1995-10-16 00:00:00

  • Identification of succinate exporter in Corynebacterium glutamicum and its physiological roles under anaerobic conditions.

    abstract::Corynebacterium glutamicum produces succinate from glucose via the reductive tricarboxylic acid cycle under microaerobic and anaerobic conditions. We identified a NCgl2130 gene of C. glutamicum as a novel succinate exporter that functions in succinate production, and designated sucE1. sucE1 expression levels were high...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Fukui K,Koseki C,Yamamoto Y,Nakamura J,Sasahara A,Yuji R,Hashiguchi K,Usuda Y,Matsui K,Kojima H,Abe K

    更新日期:2011-06-10 00:00:00

  • Impact of different CO2/HCO3- levels on metabolism and regulation in Corynebacterium glutamicum.

    abstract::We investigated the growth kinetics and transcriptional responses of Corynebacterium glutamicum in environments with low (pCO2<40 mbar) and high (pCO2 ≥ 300 mbar) CO2/HCO3(-) levels compared to standard conditions. When cultivated at high CO2/HCO3(-)-levels, C. glutamicum showed increased (63%) biomass to substrate yi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Blombach B,Buchholz J,Busche T,Kalinowski J,Takors R

    更新日期:2013-12-01 00:00:00

  • Co-expression of disulfide oxidoreductases DsbA/DsbC markedly enhanced soluble and functional expression of reteplase in Escherichia coli.

    abstract::Reteplase is the third generation of thrombolytic medicine and has many advantages over commercial t-PA. However, over-expressing recombinant reteplase in E. coli always accumulates as inclusion bodies due to nine pairs of disulfide bonds formation that is the main obstacle for correct folding. In this paper, in order...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Zhuo XF,Zhang YY,Guan YX,Yao SJ

    更新日期:2014-12-20 00:00:00

  • A comparative study of structure, stability and function of sc-tenecteplase in the presence of stabilizing osmolytes.

    abstract::The aim of the present study was to investigate the effect of three routine drug excipients, as osmolytes, in three different concentrations, on structure, thermal stability and the activity of single-chain (sc-) tenecteplase. To see the influence of trehalose, mannitol, and sucrose on the structure, stability and func...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Bayat M,Gourabi H,Khammari A,Ahmad F,Saboury AA

    更新日期:2018-08-20 00:00:00

  • Optimization of lactic acid production using immobilized Lactobacillus Rhamnosus and carob pod waste from the Lebanese food industry.

    abstract::The valorization of a solid carob waste from the Lebanese industry was investigated by optimizing the production of lactic acid using immobilized Lactobacillus rhamnosus in alginate beads and response surface methodology. The results showed that pH and alginate concentration had a significant effect on the production ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Bahry H,Abdalla R,Pons A,Taha S,Vial C

    更新日期:2019-12-20 00:00:00

  • Integration of distributed multi-analyzer monitoring and control in bioprocessing based on a real-time expert system.

    abstract::A computer system solution for integration of a distributed bioreactor monitoring and control instrumentation on the laboratory scale is described. Bioreactors equipped with on-line analyzers for mass spectrometry, near-infrared spectroscopy, electrochemical probes and multi-array gas sensors and their respective soft...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Cimander C,Bachinger T,Mandenius CF

    更新日期:2003-08-15 00:00:00

  • Continuous gas fermentation by Acetobacterium woodii in a submerged membrane reactor with full cell retention.

    abstract::Acetogenic bacteria like Acetobacterium woodii represent an ancient group of anaerobic microorganisms which use hydrogen and carbon dioxide to produce acetate. Cell concentrations and space-time yields are usually low in gas fermentations. A standard stirred‑tank bioreactor with continuous gas supply was equipped with...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Kantzow C,Mayer A,Weuster-Botz D

    更新日期:2015-10-20 00:00:00

  • Comparative and functional genomics of Listeria spp.

    abstract::The genus Listeria comprises a group of non-sporulating, Gram-positive, soil bacteria belonging to the low G+C group of microorganisms. The genus consists of only six species, L. monocytogenes, L. ivanovii, L. seeligeri, L. innocua, L. welshimeri, and L. grayi.L. monocytogenes and L. ivanovii are the only known pathog...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Hain T,Steinweg C,Chakraborty T

    更新日期:2006-10-20 00:00:00

  • Complete genome sequence of novel carbon monoxide oxidizing bacteria Citrobacter amalonaticus Y19, assembled de novo.

    abstract::We report here the complete genome sequence of Citrobacter amalonaticus Y19 isolated from an anaerobic digester. PacBio single-molecule real-time (SMRT) sequencing was employed, resulting in a single scaffold of 5.58Mb. The sequence of a mega plasmid of 291Kb size is also presented. ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Ainala SK,Seol E,Park S

    更新日期:2015-10-10 00:00:00

  • Hydroperoxide-lyase activity in mint leaves. Volatile C6-aldehyde production from hydroperoxy-fatty acids.

    abstract::The extraction of 13-hydroperoxide-lyase activity from mint leaves as well as its use for C6-aldehyde production was studied in this work. The enzyme cleaves 13(S)-hydroperoxy-C18 fatty acids into C6-aldehyde and C12-oxo-acid. Two mint species were tested: Mentha veridis and Mentha pulegium. The headspace injection me...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Gargouri M,Drouet P,Legoy MD

    更新日期:2004-07-01 00:00:00

  • New N-acyl-D-glucosamine 2-epimerases from cyanobacteria with high activity in the absence of ATP and low inhibition by pyruvate.

    abstract::N-Acetylneuraminic acid, an important component of glycoconjugates with various biological functions, can be produced from N-acetyl-D-glucosamine (GlcNAc) and pyruvate using a one-pot, two-enzyme system consisting of N-acyl-D-glucosamine 2-epimerase (AGE) and N-acetylneuraminate lyase (NAL). In this system, the epimer...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Klermund L,Groher A,Castiglione K

    更新日期:2013-11-01 00:00:00

  • Analysis of reduction of Geobacillus stearothermophilus spores treated with high hydrostatic pressure and mild heat in milk buffer.

    abstract::Our unpublished experimental results of fractional factorial experiments showed that the significant external factors affecting high pressure processing (HPP) inactivation were pressure, temperature and pressure holding time. Based on these results, response surface methodology (RSM) was employed in the present work a...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Gao YL,Ju XR,Jiang HH

    更新日期:2006-09-18 00:00:00

  • The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging.

    abstract::Super-resolution fluorescence imaging methods based on reversible photoswitching of fluorophores with subsequent localization currently develop to promising tools for cellular imaging. Since most of these methods rely on the transfer of the majority of fluorophores to a non-fluorescent dark state and precise localizat...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: van de Linde S,Wolter S,Heilemann M,Sauer M

    更新日期:2010-09-15 00:00:00

  • Complete genome sequence of Pedobacter cryoconitis PAMC 27485, a CRISPR-Cas system-containing psychrophile isolated from Antarctica.

    abstract::Pedobacter cryoconitis PAMC 27485, an aerobic, Gram-negative, facultatively psychrophilic bacterium, was isolated from Antarctic soil. Here we report the complete genome of P. cryoconitis PAMC 27485, which contains a type II CRISPR-Cas system and genes encoding useful enzymes (e.g. proteases). The genome sequence of P...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lee J,Jung YJ,Lee HK,Hong SG,Kim OS

    更新日期:2016-05-20 00:00:00

  • Construction of a bioreactor to produce special breakdown products of phytate.

    abstract::Escherichia coli phytase was covalently immobilized on NHS-activated Sepharose High Performance. The pH dependence of the phytase activity was not influenced by immobilization, whereas stability against heat treatment was enhanced as a consequence of immobilization. Compared to the free phytase the immobilized enzyme ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Greiner R,Konietzny U

    更新日期:1996-07-18 00:00:00

  • Oligonucleotide array for detection of common severe determinants of alpha thalassemia.

    abstract::A simple and an efficient oligonucleotide array was developed to identify common severe determinants of alpha (alpha) thalassemia. A total of 14 probes were designed to detect the most frequently three deletions (-alpha(3.7), -alpha(4.2), -(SEA)) and two non-deletions (alpha(Quong Sze), alpha(Constant Spring)). PCR pr...

    journal_title:Journal of biotechnology

    pub_type: 临床试验,杂志文章


    authors: Ye BC,Zhang Z,Lei Z

    更新日期:2005-01-12 00:00:00

  • The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology.

    abstract::Composition and gene content of a biogas-producing microbial community from a production-scale biogas plant fed with renewable primary products was analysed by means of a metagenomic approach applying the ultrafast 454-pyrosequencing technology. Sequencing of isolated total community DNA on a Genome Sequencer FLX Syst...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Schlüter A,Bekel T,Diaz NN,Dondrup M,Eichenlaub R,Gartemann KH,Krahn I,Krause L,Krömeke H,Kruse O,Mussgnug JH,Neuweger H,Niehaus K,Pühler A,Runte KJ,Szczepanowski R,Tauch A,Tilker A,Viehöver P,Goesmann A

    更新日期:2008-08-31 00:00:00

  • Expression and characterization of a chimeric bispecific antibody against fibrin and against urokinase-type plasminogen activator.

    abstract::We have produced a chimeric bispecific antibody that has dual specificity of human fibrin and urokinase-type plasminogen activator (u-PA). Complementary DNAs for variable regions of both anti-fibrin and anti-u-PA antibodies were cloned from two murine hybridomas secreting respective antibodies using polymerase chain r...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Tada H,Kurokawa T,Seita T,Watanabe T,Iwasa S

    更新日期:1994-03-31 00:00:00

  • Development of an in vivo methylation system for the solventogen Clostridium saccharobutylicum NCP 262 and analysis of two endonuclease mutants.

    abstract::The genome of the biotechnologically important solventogenic Clostridium saccharobutylicum NCP 262 contains two operons coding for genes of presumed type I RM systems belonging to the families A and C. They represent a limiting factor for the development of transformation and conjugation protocols. We established an e...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Lesiak JM,Liebl W,Ehrenreich A

    更新日期:2014-10-20 00:00:00

  • Characterization of a Trichoplusia ni hexamerin-derived promoter in the AcMNPV baculovirus vector.

    abstract::The promoter sequences of the encoding genes for the three most abundant hexamerins of the Lepidoptera Trichoplusia ni were isolated and cloned into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-derived baculovirus expression vector. From the sequences analyzed, the DNA region driving the expressio...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: López-Vidal J,Gómez-Sebastián S,Sánchez-Ramos I,Escribano JM

    更新日期:2013-06-10 00:00:00

  • Antigen recognition characteristics and comparative performance in immunoaffinity purification of two monoclonal antibodies specific for the hepatitis B virus surface antigen.

    abstract::In this paper we describe the antigen recognition characteristics, variable region base and amino acid sequence, and performance as immunoaffinity chromatography ligands of two MAb specific to the alpha determinant of the HBsAg, derived from the same fusion. We show that the epitope recognized by CB-Hep.0 (IgM) is pro...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Fernández de Cossío ME,Díaz T,Galván A,Valdéz R,González E,Ayala M,Díaz J,Bestagno M,Burrone O,Gavilondo J

    更新日期:1997-08-11 00:00:00

  • Dual nutrient limited growth: models, experimental observations, and applications.

    abstract::Dual nutrient limited growth, the control of the cell growth rate (kinetic aspect) or the restriction of the amount of biomass (stoichiometric aspect) by two nutrients at the same time, is a relatively unknown ability of the microorganisms and consequently, still not mentioned in textbooks to date. Nevertheless, multi...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: Zinn M,Witholt B,Egli T

    更新日期:2004-09-30 00:00:00

  • Functional genomic analysis of the Bacillus subtilis Tat pathway for protein secretion.

    abstract::Protein secretion from Bacillus species is a major industrial production tool with a market of over $1 billion per year. However, standard export technologies, based on the well-characterised general secretory (Sec) pathway, are frequently inapplicable for the production of proteins. The recently discovered twin-argin...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章,评审


    authors: van Dijl JM,Braun PG,Robinson C,Quax WJ,Antelmann H,Hecker M,Müller J,Tjalsma H,Bron S,Jongbloed JD

    更新日期:2002-09-25 00:00:00

  • A novel direct screening method for alkyl glucoside production by glucosidases expressed in E. coli in 96-well plates.

    abstract::The present work describes the development of a novel direct screening method, assayed in 96-well format, for evaluation of enzymatic alkyl glycoside production in a hexanol-water two-phase system. Alkyl glycosides are surfactants with a range of applications and with good biodegradability and low toxicity. Enzymatic ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Gräber M,Andersson M,Rundbäck F,Pozzo T,Karlsson EN,Adlercreutz P

    更新日期:2010-01-15 00:00:00

  • Induced prodrug activation by conditional protein degradation.

    abstract::Enzyme prodrug therapies hold potential as a targeted treatment option for cancer patients. However, off-target effects can be detrimental to patient health and represent a safety concern. This concern can be alleviated by including a failsafe mechanism that can abort the therapy in healthy cells. This feature can be ...

    journal_title:Journal of biotechnology

    pub_type: 杂志文章


    authors: Gaynor AS,Chen W

    更新日期:2017-10-20 00:00:00