Abstract：
:A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was further demethylated to protocatechuic acid. Above 95% substrate degradation was obtained within 7 h of growth on ferulic acid medium, which is the shortest period of time reported to date. The major degradation products, was isolated and identified by thin-layer chromatography, high performance liquid chromatography and 1H-nuclear magnetic resonance spectroscopy were 4-vinylguaiacol, vanillin, vanillic acid and protocatechuic acid.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Karmakar B,Vohra RM,Nandanwar H,Sharma P,Gupta KG,Sobti RCdoi
10.1016/s0168-1656(00)00248-0subject
Has Abstractpub_date
2000-07-14 00:00:00pages
195-202issue
3eissn
0168-1656issn
1873-4863pii
S0168165600002480journal_volume
80pub_type
杂志文章abstract：:Dual nutrient limited growth, the control of the cell growth rate (kinetic aspect) or the restriction of the amount of biomass (stoichiometric aspect) by two nutrients at the same time, is a relatively unknown ability of the microorganisms and consequently, still not mentioned in textbooks to date. Nevertheless, multi...
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