Markerless genome editing in Clostridium beijerinckii using the CRISPR-Cpf1 system.

abstract：:There has been little research reported on the synergism of plant expansins in cellulose hydrolysis because of the difficulty of heterologous expression. In this study, the expansin gene LeExp2 of Lycopersicum esculentum was expressed for the first time in Pichia pastoris to identify its function in vitro. The optimal...

journal_title：Journal of biotechnology

authors： Liu X,Liu C,Ma Y,Hong J,Zhang M

更新日期：2014-09-30 00:00:00

abstract：:Old yellow enzymes (OYEs, EC 1.6.99.1) are flavin-dependent oxidoreductases that catalyze the stereoselective trans-hydrogenation of the double bond, representing a promising alternative to metal-based catalysis. Bioconversion of ketoisophorone (KIP) by 28 non-conventional yeasts belonging to 16 different species was ...

journal_title：Journal of biotechnology

authors： Raimondi S,Romano D,Amaretti A,Molinari F,Rossi M

更新日期：2011-12-20 00:00:00

abstract：:The Pol6 mutant of Penicillium occitanis, secreting a large quantity of cellulases, was cultivated in fermentor using a local paper pulp as an inducer substrate. A high titer of extracellular cellulase activity was reached after a fed batch process: 23 IU x ml(-1) filter paper activity, 21 IU x ml(-1) CMCases activity...

journal_title：Journal of biotechnology

authors： Belghith H,Ellouz-Chaabouni S,Gargouri A

更新日期：2001-08-23 00:00:00

abstract：:Randomly amplified polymorphic DNA (RAPD) was used to analyzed 78 samples comprises of certified reference materials (soya and maize powder), raw seeds (soybean and maize), processed food and animal feed. Combination assay of two arbitrary primers in the RAPD analysis enable to distinguish genetically modified organis...

journal_title：Journal of biotechnology

authors： Yoke-Kqueen C,Radu S

更新日期：2006-12-15 00:00:00

abstract：:The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). T...

journal_title：Journal of biotechnology

authors： Schenk JA,Fettke J,Lenz C,Albers K,Mallwitz F,Gajovic-Eichelmann N,Ehrentreich-Förster E,Kusch E,Sellrie F

更新日期：2012-03-31 00:00:00

abstract：:Rice straw is a by-product of rice production, and a great bioresource as raw biomass material for manufacturing value-adding protein for animal feedstock, which has been paid more and more attention. In the present work, utilizing rice straw hydrolysate as a substrate for microbial biomass production in 11.5L externa...

journal_title：Journal of biotechnology

authors： Zheng YG,Chen XL,Wang Z

更新日期：2005-09-10 00:00:00

abstract：:Vacuolar sequestration of valuable secondary metabolites remains the major limitation to the use of immobilization technology for large scale plant-cell-based bioprocesses, which otherwise may be a more efficient culture system than suspension for this biomass. In this initial study, the release of indole alkaloids pr...

journal_title：Journal of biotechnology

authors： Jardin B,Tom R,Chavarie C,Rho D,Archambault J

更新日期：1991-11-01 00:00:00

abstract：:The stability, copy number, and gene expression of the pBR322 plasmid containing the par-locus under prolonged cultivation were studied. In the initial stage of the experiment it was observed that the par-locus had a stabilization effect on plasmid maintenance. This observation was consistent with previously reported ...

journal_title：Journal of biotechnology

authors： Weber AE,Yu P,San KY

更新日期：1991-04-01 00:00:00

abstract：:In this paper, we report the chromium(VI) reduction by filtrates of Acidithiobacillus and Thiobacillus cultures. Chromium(VI) reduction by filtrates of A. ferrooxidans cultures under acidic conditions was higher than that observed for A. thiooxidans. However, at pH close to 7, chromium(VI) reduction by filtrates of T....

journal_title：Journal of biotechnology

authors： Allegretti P,Furlong J,Donati E

更新日期：2006-03-09 00:00:00

abstract：:Protein engineering has been employed to investigate the effect of specific amino acid changes on the targeting of heterologous proteins to the outer cell surface of the Gram-positive bacterium Staphylococcus xylosus. Three different variants, corresponding to a 101 amino acid region of the major glycoprotein (G prote...

journal_title：Journal of biotechnology

authors： Nguyen TN,Gourdon MH,Hansson M,Robert A,Samuelson P,Libon C,Andréoni C,Nygren PA,Binz H,Uhlén M

更新日期：1995-10-16 00:00:00

abstract：:Microbiologically induced calcium carbonate precipitation shows the potential for use in bioremediation and construction consolidation, but the efficiency of this process must be improved. Lysinibacillus sphaericus LMG 22257 is a gram-positive ureolytic strain that has recently been applied for consolidating construct...

journal_title：Journal of biotechnology

authors： Yan W,Xiao X,Zhang Y

更新日期：2017-03-20 00:00:00

abstract：:Oleaginous microalgae have received a considerable attention as potential biofuel feedstock. However, lack of industry-suitable strain with lipid rich biomass limits its commercial applications. Targeted engineering of lipogenic pathways represents a promising strategy to enhance the efficacy of microalgal oil product...

journal_title：Journal of biotechnology

authors： Li DW,Cen SY,Liu YH,Balamurugan S,Zheng XY,Alimujiang A,Yang WD,Liu JS,Li HY

更新日期：2016-07-10 00:00:00

abstract：:Sugarcane bagasse (SCB) is an abundant, renewable and inexpensive agricultural byproduct for the production of biofuel and other biobased products. To effectively saccharify SCB with cellulases, combination with dilute alkali salts Na2SO3/Na3PO4 (0.4% Na3PO4, 0.03% Na2SO3) at 7.5% sulfidity and hot water (DASHW) in "o...

journal_title：Journal of biotechnology

authors： Jiang CX,He YC,Chong GG,Di JH,Tang YJ,Ma CL

更新日期：2017-10-10 00:00:00

abstract：:Lactulose and galacto-oligosaccharides (GOS) are well recognized prebiotics derived from lactose. In the synthesis of lactulose with β-galactosidases GOS are also produced, but the ratio of lactulose and GOS in the product can be tuned at will, depending on the operation conditions, so to obtain an optimal product dis...

journal_title：Journal of biotechnology

authors： Guerrero C,Vera C,Acevedo F,Illanes A

更新日期：2015-09-10 00:00:00

abstract：:This paper introduces a novel approach to enhance the strains' biodegradation of 4-chloronitrobenzene by utilizing the synergistic effect of the organic reductant mannitol and the substrate beef extraction. Our results demonstrate that 4-chloronitrobenzene could not be an available nitrogen source to support target st...

journal_title：Journal of biotechnology

authors： Li T,Zhang TC,He L

更新日期：2017-12-20 00:00:00

abstract：:The simultaneous biosynthesis of lovastatin (mevinolinic acid) and (+)-geodin by Aspergillus terreus ATCC 20542 with regard to the medium composition, i.e. the concentrations of carbon and nitrogen source, was described in this paper. A. terreus is a lovastatin producer but the formation of lovastatin was accompanied ...

journal_title：Journal of biotechnology

authors： Bizukojc M,Ledakowicz S

更新日期：2007-12-01 00:00:00

abstract：:The enzymatic Pictet-Spengler (PS) reaction, catalyzed by Pictet-Spenglerase (PSase), is the feature of β-carboline (βC) alkaloid biosynthesis. NscbB is a rare microbial PSase discovered from a cryptic β-carboline alkaloid biosynthetic gene cluster (BGC) in the Nocardiopsis synnemataformans DSM 44143 (kidney transplan...

journal_title：Journal of biotechnology

authors： Chen Q,Zhang S,Xie Y

更新日期：2018-09-10 00:00:00

abstract：:As the important chiral building block of levetiracetam, the synthesis of (S)-2-aminobutanamide has attracted a great deal of attention. The d-aminopeptidase catalyzed kinetic resolution of 2-aminobutanamide was demonstrated as an effective strategy for (S)-2-aminobutanamide production. In this study, a novel d-aminop...

journal_title：Journal of biotechnology

authors： Tang XL,Lu XF,Wu ZM,Zheng RC,Zheng YG

更新日期：2018-01-20 00:00:00

abstract：:Kinetic models have a great potential for metabolic engineering applications. They can be used for testing which genetic and regulatory modifications can increase the production of metabolites of interest, while simultaneously monitoring other key functions of the host organism. This work presents a methodology for in...

journal_title：Journal of biotechnology

authors： Villaverde AF,Bongard S,Mauch K,Balsa-Canto E,Banga JR

更新日期：2016-03-20 00:00:00

abstract：:This work explores some of the possibilities to replace human cytomegalovirus (CMV) core and/or enhancer promoter control elements to create new expression vectors for use with fish. The work is relevant to fish vaccination, since DNA vaccines use eukaryotic expression plasmids controlled by the human cytomegalovirus ...

journal_title：Journal of biotechnology

authors： Martinez-Lopez A,Chinchilla B,Encinas P,Gomez-Casado E,Estepa A,Coll JM

更新日期：2012-12-15 00:00:00

abstract：:Understanding the causes of discrepancies in pH readings of a sample can allow more robust pH control strategies to be implemented. It was found that 59.4% of differences between two offline pH measurement technologies for an historical dataset lay outside an expected instrument error range of ±0.02pH. A new variable,...

journal_title：Journal of biotechnology

authors： Ritchie EK,Martin EB,Racher A,Jaques C

更新日期：2017-06-10 00:00:00

abstract：:A novel expression vector for yeast has been constructed from the regulatory elements present in the polI promoter and the enhancer/termination region (E/T) of rDNA. Under some conditions, this promoter/vector combination produces small RNAs such as the hammerhead RNA sequence at levels comparable to polII- and polIII...

journal_title：Journal of biotechnology

authors： Blancafort P,Ferbeyre G,Sariol C,Cedergren R

更新日期：1997-07-23 00:00:00

abstract：:Microbiologically induced calcium carbonate precipitation (MICCP) is a naturally occurring biological process that has shown its potential in remediation of a wide range of structural damages including concrete cracks. In this study, genetically engineered microorganisms, capable of producing extracellular polymeric s...

journal_title：Journal of biotechnology

authors： Bergdale TE,Pinkelman RJ,Hughes SR,Zambelli B,Ciurli S,Bang SS

更新日期：2012-10-31 00:00:00

abstract：:Aqueous two-phase systems allow for the unequal distribution of proteins and other molecules in water-rich solutions containing phase separating polymers or surfactants. One approach to improve the partitioning properties of recombinant proteins is to produce the proteins as fused to certain peptide tags. However, the...

journal_title：Journal of biotechnology

authors： Bandmann N,Van Alstine J,Veide A,Nygren PA

更新日期：2002-01-31 00:00:00

abstract：:To explore the utility of glycosyltransferases as novel biocatalysts, we isolated the glycosyltransferase genes CaUGT2 and SbUGTA1 from Catharanthus roseus and Starmerella bombicola, respectively and heterologously expressed them in Escherichia coli. The purified recombinant proteins were assayed with a variety of sma...

journal_title：Journal of biotechnology

authors： Huang FC,Hinkelmann J,Schwab W

更新日期：2015-12-20 00:00:00

abstract：:A murine B-lymphocyte hybridoma (CC9C10) was adapted for growth in a serum-free medium. Supplementation of the medium with cis-unsaturated fatty acids (10-50 microM) improved the cell yield in the order oleic/linoleic > linoleic > oleic. Initial supplementation with the fatty acids also caused a significant increase (...

journal_title：Journal of biotechnology

authors： Butler M,Huzel N

更新日期：1995-04-15 00:00:00

abstract：:The hematopoietic system represents an interesting model for gene transfer protocols. Here, we have evaluated the efficiency of a gene transfer system using the polycationic compound SuperFect (Qiagen) and the K562 hematopoietic cell line. Transient and stable vectors carrying the enhanced green fluorescent protein (E...

journal_title：Journal of biotechnology

authors： Teixeira LA,Fricke CH,Bonorino CB,Bogo MR,Nardi NB

更新日期：2001-06-15 00:00:00

abstract：:The combination of anaerobic and aerobic periods in the operation cycle of a Sequencing Batch Reactor (SBR) was chosen to study biological color removal from simulated textile effluents containing reactive, sulfonated, monoazo and diazo dyes, respectively, Remazol Brilliant Violet 5R and Remazol Black B. 90% color rem...

journal_title：Journal of biotechnology

authors： Lourenço ND,Novais JM,Pinheiro HM

更新日期：2001-08-23 00:00:00

abstract：:The unsaturations and groups bound to the ring and to the lateral chain of lactones give a large diversity in this class of molecules. In this work we produced enzymatically a macrolactone in organic media. The substrate used was a hydroxy-fatty acid: (+)-coriolic acid and the enzymes tested were free or immobilized m...

journal_title：Journal of biotechnology

authors： Gargouri M,Drouet P,Legoy MD

更新日期：2002-01-18 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) is the primary causative agent of an economically important swine disease, now known as porcine-associated disease (PCVAD). The only structural protein of viral capsid, Cap has become the major target for development of PCV2 subunit vaccines. The purpose of this study is to express Cap...

journal_title：Journal of biotechnology

authors： Chi JN,Wu CY,Chien MS,Wu PC,Wu CM,Huang C

更新日期：2014-07-10 00:00:00