Abstract：
:There has been little research reported on the synergism of plant expansins in cellulose hydrolysis because of the difficulty of heterologous expression. In this study, the expansin gene LeExp2 of Lycopersicum esculentum was expressed for the first time in Pichia pastoris to identify its function in vitro. The optimal expression level was obtained by adding PMSF and a commercial protease inhibitor, and the maximum expression level was 70.9 mg/L. The affinity-purified LeEXP2 displayed cellulose-weakening activity and synergism with cellulase, and the reducing sugar yield in the reaction mixture with LeEXP2 was 1.4-9.8-fold that of control. The optimum pH and temperature for reducing-sugar liberation by LeEXP2 and endoglucanase were pH 4.8 and 50 °C. Cu(2+) and Fe(2+) notably increased the synergistic activity of LeEXP2, and Co(2+) enhanced the activity of endoglucanase but failed to improve synergism. LeEXP2 exhibited a high level of robustness to reagents and heat, indicating its potential application for cellulose decomposition. LeEXP2 preferentially bound to cellulose over lignin, and the binding capability to given substrates was not related to the crystallinity. This is the first study to characterize the functional role of LeEXP2 in cellulose degradation.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Liu X,Liu C,Ma Y,Hong J,Zhang Mdoi
10.1016/j.jbiotec.2014.07.013subject
Has Abstractpub_date
2014-09-30 00:00:00pages
148-55eissn
0168-1656issn
1873-4863pii
S0168-1656(14)00349-6journal_volume
186pub_type
杂志文章abstract：:Aspergillus niger AB1.13 cultures with glucoamylase production (with D-glucose as substrate) and without glucoamylase production (with D-xylose as substrate) were characterized by metabolic flux analysis. Two comprehensive metabolic models for d-glucose- as well as for D-xylose-consumption were used to quantify and co...
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