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Development of stem borer resistant transgenic parental lines involved in the production of hybrid rice.

Abstract：

:Stem borer resistant transgenic parental lines, involved in hybrid rice, were produced by Agrobacterium-mediated gene transfer method. Two pSB111 super-binary vectors containing modified cry1Ab/cry1Ac genes driven by maize ubiquitin promoter, and herbicide resistance gene bar driven by cauliflower mosaic virus 35S promoter were, used in this study. Embryogenic calli after co-cultivation with Agrobacterium were selected on the medium containing phosphinothricin. Southern blot analyses of primary transformants revealed the stable integration of bar, cry1Ab and cry1Ac coding sequences into the genomes of three parental lines with a predominant single copy integration and without any rearrangement of T-DNA. T1 progeny plants disclosed a monogenic pattern (3:1) of transgene segregation as confirmed by molecular analyses. Furthermore, the co-segregation of bar and cry genes in T1 progenies suggested that the transgenes are integrated at a single site in the rice genome. In different primary transformants with alien inbuilt resistance, the levels of cry proteins varied between 0.03 and 0.13% of total soluble proteins. These transgenic lines expressing insecticidal proteins afforded substantial resistance against stem borers. This is the first report of its kind dealing with the introduction of Bacillus thuringiensis (Bt) cry genes into the elite parental lines involved in the development of hybrid rice.

journal_name

J Biotechnol

journal_title

Journal of biotechnology

authors

Ramesh S,Nagadhara D,Pasalu IC,Kumari AP,Sarma NP,Reddy VD,Rao KV

doi

10.1016/j.jbiotec.2004.04.004

subject

Has Abstract

pub_date

2004-07-15 00:00:00

pages

131-41

issue

2

eissn

0168-1656

issn

1873-4863

pii

S0168165604001907

journal_volume

111

pub_type

杂志文章

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