Abstract:
:Glutamate dehydrogenase is a central enzyme connecting nitrogen and carbon metabolism via its precursors ammonium and oxoglutarate and its product glutamate. In Corynebacterium glutamicum glutamate dehydrogenase is especially important, since it is a key enzyme for the biotechnological production of the flavour enhancer L-glutamate. In this study, the regulation of gdh transcription was investigated. While originally described as a constitutively expressed gene, we could show that gdh transcription is highly variable depending on environmental conditions. Regulation of the gdh gene by the C. glutamicum nitrogen control protein AmtR was analyzed in detail in this study.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Hänssler E,Müller T,Palumbo K,Patek M,Brocker M,Krämer R,Burkovski Adoi
10.1016/j.jbiotec.2009.04.007subject
Has Abstractpub_date
2009-06-15 00:00:00pages
114-22issue
2eissn
0168-1656issn
1873-4863pii
S0168-1656(09)00179-5journal_volume
142pub_type
杂志文章abstract::New emerging technologies delivering benefits in terms of process robustness and economy are an inevitable prerequisite for monoclonal antibody purification processes intensification. Caprylic acid was proven as an effective precipitating agent enabling efficient precipitaton of product- and process-related impurities...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2018.05.001
更新日期:2018-08-10 00:00:00
abstract::Native enzyme and a mutant containing an extra disulphide bridge of recombinant Saccharomycopsis fibuligera R64 α-amylase, designated as Sfamy01 and Sfamy02, respectively, have successfully been overexpressed in the yeast Pichia pastoris KM71H. The purified α-amylase variants demonstrated starch hydrolysis resulting i...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2014.12.002
更新日期:2015-02-10 00:00:00
abstract::A gene encoding mature human insulin-like growth factor II (IGF-II) was constructed from the modified IGF-II cDNA sequence and two double-stranded synthetic oligodeoxynucleotide linkers. It was fused to a truncated lacZ gene such that IGF-II was expressed as part of C-terminus of beta-galactosidase. This fused lacZ'-I...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/0168-1656(90)90077-o
更新日期:1990-03-01 00:00:00
abstract::Sulphite spent liquor (SSL) is a side product from acidic sulphite pulping of wood, which organic counterpart is composed mainly by lignosulphonates (LS) and sugars. The last are a prominent substrate for the bioprocessing although a previous purification step is necessary to eliminate microbial inhibitors. In this st...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2012.03.013
更新日期:2012-12-31 00:00:00
abstract::Overcoming the effects of hydrolysate toxicity towards ethanologens is a key technical barrier in the biochemical conversion process for biomass feedstocks to ethanol. Despite its importance, the complexity of the hydrolysate toxicity phenomena and the lack of systematic studies, analysis and tools surrounding this is...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2009.08.006
更新日期:2009-12-01 00:00:00
abstract::Serial repitching of brewing yeast inoculates is an important economic factor in the brewing industry, as their propagation is time and resource intensive. Here, we investigated whether replicative aging and/or the population distribution status changed during serial repitching in three different breweries with the sa...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2014.07.002
更新日期:2014-10-10 00:00:00
abstract::The cDNA coding for human proapolipoprotein A-I was expressed as a ubiquitin fusion under the control of the polyhedrin promoter in baculovirus-infected Sf9 Spodoptera frugiperda insect cells. The fusion protein was expressed at high level and was quantitatively cleaved in vivo. The cleaved product was purified and it...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/0168-1656(94)90118-x
更新日期:1994-01-15 00:00:00
abstract::Cell wall integrity signaling pathway in Saccharomyces cerevisiae is a conserved function for detecting and responding to cell stress conditions but less understood for industrial yeast. We examined gene expression dynamics for a tolerant industrial yeast strain NRRL Y-50049 in response to challenges of furfural and H...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2018.04.002
更新日期:2018-06-20 00:00:00
abstract::Protein expression of Spodoptera frugiperda (Sf9) insect cells was characterized upon exposure to environmental stresses typically present in bioreactors including heat shock, oxygen deprivation, shear stress, change of pH, and salinity or ethanol shock. This study fills the void in knowledge as to how bioreactor hydr...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2005.05.009
更新日期:2005-08-22 00:00:00
abstract::Genetically engineered human osteosarcoma cells containing developmental endothelial locus-1 (del-1) gene were studied for production of Del-1, a protein that has the properties of an extracellular matrix protein and can regulate vascular morphogenesis and remodeling. Del-1 has been studied as a potential anti-angioge...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(02)00053-6
更新日期:2002-07-17 00:00:00
abstract::Terminal sialic acid on oligosaccharides of glycoproteins shows several biological functions of the glycoproteins. The yeast Pichia pastoris normally does not contain sialic acid on the oligosaccharides of glycoproteins. A sialyltransferase (ST) gene was transfected into P. pastoris to assess the possibility of using ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(00)00268-6
更新日期:2000-07-28 00:00:00
abstract::The pyranose 2-oxidases from Trametes ochracea and Trametes pubescens share markedly similar amino acid sequences with identity of 93.4%. When expressed from the recombinant plasmids based on the same vector in the Escherichia coli host strain BL21(DE3) at higher growth temperatures, they differ strikingly in the form...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2007.04.023
更新日期:2007-06-30 00:00:00
abstract::Great interest has emerged in the recent past towards the potential of autotrophic acetogenic bacteria for the sustainable production of fuels and chemicals. This group of microorganisms possesses an ancient pathway for the fixation of carbon dioxide in the presence of hydrogen, making them highly attractive for the u...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2014.03.005
更新日期:2014-05-20 00:00:00
abstract::Harbour sediments are periodically subjected to dredging operations and their management is mainly based on the assessment of the chemical contamination levels, but the potential risks posed by the presence of pathogenic microorganisms have been largely neglected. Here we first developed new molecular protocols based ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2011.07.033
更新日期:2012-02-20 00:00:00
abstract::The fed-batch process for commercial production of riboflavin (vitamin B2) was optimized on-line using model-predictive control based on artificial neural networks (ANNs). The information required for process models was extracted from both historical data and heuristic rules. After each cultivation the process model w...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(00)00211-x
更新日期:2000-04-14 00:00:00
abstract::Aspergillus oryzae finds wide application in the food, feed, and wine industries, and is an excellent cell factory platform for production of organic acids. In this work, we achieved the overproduction of L-malate by rewiring the reductive tricarboxylic acid (rTCA) pathway and L-malate transport pathway of A. oryzae N...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2017.05.011
更新日期:2017-07-10 00:00:00
abstract::To improve enzymatic activity of Bacillus pumilus lipases, DNA shuffling was applied to two lipase genes from local B. pumilus isolates. Using a high-throughput activity assay, the mutant with highest activity was selected. This chimeric mutant (L3-3), carrying two crossover positions and three point mutations, has a ...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2012.12.016
更新日期:2013-03-10 00:00:00
abstract::Threonine aldolase (TA) catalyzes a reversible reaction, in which threonine is decomposed into glycine and acetaldehyde. The same enzyme can be used to catalyze aldol reaction between glycine and a variety of aromatic and aliphatic aldehydes, thus creating various alpha-amino-alcohols. Therefore, TA is a very promisin...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2015.02.013
更新日期:2015-04-10 00:00:00
abstract::Based on the prototype experiment of treating herb wastewater by Up flow Anaerobic Sludge Bed and Anaerobic Filter reactor (UASBAF), an artificial neural network (ANN) model which adopts a back propagation algorithm with momentum and adaptive learning rate was established. And the effect of each parameter to the perfo...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2009.08.014
更新日期:2009-10-12 00:00:00
abstract::Oxygen is a key substrate in animal cell metabolism. It has been reported that the oxygen uptake rate (OUR) is a good indicator of cellular activity, and even under some conditions, a good indicator of the number of viable cells. The measurement of OUR is difficult due to many different reasons. In particular, the ver...
journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0168-1656(98)00046-7
更新日期:1998-08-12 00:00:00
abstract::Plasmid instability of a recombinant Saccharomyces cerevisiae C468/pGAC9 (ATCC 20690) was examined during continuous culture in a nonselective medium in an airlift bioreactor. The recombinant strain contained a 2-micron based shuttle vector pGAC9 and expresses Aspergillus awatnori glucoamylase gene under the control o...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(97)00057-6
更新日期:1997-05-23 00:00:00
abstract::R-lactide, a pivotal monomer for the production of poly (D-lactic acid) (PDLA) or stereocomplex poly (lactic acid) (PLA) was synthesized from alkyl (R)-lactate through a lipase-catalyzed reaction without racemization. From among several types of lipase, only lipase B from Candida antarctica (Novozym 435; CAL-B) was ef...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2013.06.021
更新日期:2013-10-20 00:00:00
abstract::E-lysis of Ralstonia eutropha H16, which belongs to the beta-subclass, was undertaken to verify whether transmembrane tunnel formation is possible in bacteria which do not belong to the enterobacteriaceae. For this purpose, a new gene E expression plasmid, pKG12, with two origins of replication, oriV and oriT, from pl...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(98)00128-x
更新日期:1998-12-11 00:00:00
abstract::To explore the utility of glycosyltransferases as novel biocatalysts, we isolated the glycosyltransferase genes CaUGT2 and SbUGTA1 from Catharanthus roseus and Starmerella bombicola, respectively and heterologously expressed them in Escherichia coli. The purified recombinant proteins were assayed with a variety of sma...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2015.10.011
更新日期:2015-12-20 00:00:00
abstract::There are currently four crystal structures of low molecular weight endo-1,4-beta-xylanases (E.C.3.2.1.8), i.e. family G/11 xylanases, available at the Brookhaven Data Bank: 2 xylanases from Trichoderma reesei (Törrönen et al., 1994; Törrönen and Rouvinen, 1995) and one from Bacillus circulans and another from Trichod...
journal_title:Journal of biotechnology
pub_type: 杂志文章,评审
doi:10.1016/s0168-1656(97)00095-3
更新日期:1997-09-16 00:00:00
abstract::The production of cephalosporin C (CPC) and its precursors penicillin N (PEN N), deacetoxycephalosporin C (DAOC) and deacetylcephalosporin C (DAC), with a highly productive strain of Cephalosporin acremonium, was investigated in an 80-1 airlift tower loop reactor with four static mixer modules (Type SMV, Sulzer) (ATLR...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/0168-1656(93)90168-m
更新日期:1993-04-01 00:00:00
abstract::Glycosyltransferase (GT)-mediated methodology is recognized as one of the most practical approaches for large-scale production of glycosides. However, GT enzymes require a sugar nucleotide as donor substrate that must be generated in situ for preparative applications by recycling of the nucleotide moiety, e.g. by sucr...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2016.02.022
更新日期:2016-04-20 00:00:00
abstract::In n-hexadecane fermentation by Pseudomonas aeruginosa, the production of rhamnolipids (biosurfactants) caused by nitrogen source limitation was observed during the stationary phase. The rhamnolipids caused severe foaming in the process, particularly at higher pH (ca. > 6.8). To reduce the foaming, several runs were m...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/s0168-1656(97)00150-8
更新日期:1997-01-03 00:00:00
abstract::The development and optimization of a purification process of monoclonal antibodies based on two continuous chromatography steps for capture and intermediate purification are presented. The two chromatography steps were individually optimized using either batch chromatography or sequential multicolumn chromatography (...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2015.04.026
更新日期:2015-11-10 00:00:00
abstract::The gene encoding histamine dehydrogenase in Rhizobium sp. 4--9 has been cloned and overexpressed in Escherichia coli. The coding region of the gene was 2,079 bp and encoded a protein of 693 amino acids with a calculated molecular mass of 76,732 Da. This histamine dehydrogenase was related to histamine dehydrogenase f...
journal_title:Journal of biotechnology
pub_type: 杂志文章
doi:10.1016/j.jbiotec.2005.04.005
更新日期:2005-09-29 00:00:00