Complete genome sequence of Pandoraea thiooxydans DSM 25325(T), a thiosulfate-oxidizing bacterium.

abstract：:This work explores the role of one of the factors explaining lipase/esterase activity: the contribution of electrostatic interactions to lipase/esterase activity. The electrostatic potential distribution on the molecular surface of an enzyme as a function of pH determines, to a large extent, the enzyme's pH activity p...

journal_title：Journal of biotechnology

authors： Neves Petersen MT,Fojan P,Petersen SB

更新日期：2001-02-13 00:00:00

abstract：:Generation and characterization of knockout clones is a widely used approach to evaluate the specific function of a gene product in Dictyostelium discoideum. The mutant clones are generally obtained by double homologous recombination in the target gene. A frequent limitation to obtaining mutants is the low frequency o...

journal_title：Journal of biotechnology

authors： Charette SJ,Cornillon S,Cosson P

更新日期：2006-03-09 00:00:00

abstract：:Human prolactin (hPRL) is a 199 aminoacid protein hormone with a wide spectrum of biological activities which is best known for its stimulation of lactation and development of mammary gland. This protein contains only one potential asparagine-linked glycosylation site, which is partially (10-30%) occupied when the pro...

journal_title：Journal of biotechnology

authors： Heller SR,Rodrigues Goulart H,Arthuso FS,Oliveira TL,Bartolini P,Soares CR

更新日期：2010-02-15 00:00:00

abstract：:The neotype strain Methanobacterium formicicum MF(T) (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074bp in size, featuring a GC content of 41.23%. M. fo...

journal_title：Journal of biotechnology

authors： Maus I,Stantscheff R,Wibberg D,Stolze Y,Winkler A,Pühler A,König H,Schlüter A

更新日期：2014-12-20 00:00:00

abstract：:Acetogenic bacteria like Acetobacterium woodii represent an ancient group of anaerobic microorganisms which use hydrogen and carbon dioxide to produce acetate. Cell concentrations and space-time yields are usually low in gas fermentations. A standard stirred‑tank bioreactor with continuous gas supply was equipped with...

journal_title：Journal of biotechnology

authors： Kantzow C,Mayer A,Weuster-Botz D

更新日期：2015-10-20 00:00:00

abstract：:Deployment of the elongated flexuous virions of Turnip mosaic virus (TuMV), a potyvirus, for peptide display on their external surface has been previously reported by us. Nonetheless, both in TuMV and other potyviruses some peptides hinder the ability of the virus to infect host plants. We found that a peptide derived...

journal_title：Journal of biotechnology

authors： González-Gamboa I,Manrique P,Sánchez F,Ponz F

更新日期：2017-07-20 00:00:00

abstract：:Genetically engineered human osteosarcoma cells containing developmental endothelial locus-1 (del-1) gene were studied for production of Del-1, a protein that has the properties of an extracellular matrix protein and can regulate vascular morphogenesis and remodeling. Del-1 has been studied as a potential anti-angioge...

journal_title：Journal of biotechnology

authors： Chen C,Huang YL,Yang ST

更新日期：2002-07-17 00:00:00

abstract：:A stably transformed insect cell expression system is superior to a baculovirus expression system, since the expression system is sustained and there is no cell lysis, but the isolation of cell lines producing recombinant proteins is time-consuming and laborious. In this study, we developed a technique for the rapid i...

journal_title：Journal of biotechnology

authors： Kato T,Yoshizuka K,Park EY

更新日期：2010-05-17 00:00:00

abstract：:In the present study, a novel strategy was adopted to synthesize, β lactamase resistant penicillin G molecules by using the unique properties of silver nanoparticles. Ascorbic acid-stabilized spherical monodispersed silver nanoparticles were prepared by a simple chemical reaction. The formation processes of the silver...

journal_title：Journal of biotechnology

authors： Ahmed V,Kumar J,Kumar M,Chauhan MB,Vij M,Ganguli M,Chauhan NS

更新日期：2013-02-20 00:00:00

abstract：:Vertebrate prostaglandin H synthases (PGHSs) are membrane-bound disulphide-containing hemoglycoproteins. Therefore, eukaryotic expression systems are required for the production of recombinant PGHSs. Recently we announced the expression of human PGHS-2 (hPGHS-2) in the yeast Pichia pastoris. Here we report improved pr...

journal_title：Journal of biotechnology

authors： Kukk K,Samel N

更新日期：2016-08-10 00:00:00

abstract：:A microorganism with the ability to form L-tryptophan from D,L-5-(3-indolyl-methyl)hydantoin (D,L-5-IMH) was isolated and identified as Arthrobacter sp. (DSM 3747). After isolation of a mutant with high tryptophan production activity but low tryptophan degradation, cultural conditions were optimized to achieve high am...

journal_title：Journal of biotechnology

authors： Syldatk C,Mackowiak V,Höke H,Gross C,Dombach G,Wagner F

更新日期：1990-06-01 00:00:00

abstract：:Aiming at maximizing the production of transmembrane rabies virus glycoprotein (rRVGP), the influence of hypothermic temperature on a recombinant Drosophila melanogaster S2 cell culture in Sf-900II medium was investigated. Cell growth and rRVGP production were assessed at 4 culture temperatures in Schott flasks: 16, 2...

journal_title：Journal of biotechnology

authors： Rossi N,Silva BG,Astray R,Swiech K,Pereira CA,Suazo CA

更新日期：2012-10-31 00:00:00

abstract：:Saccharomyces cerevisiae can be engineered for xylose fermentation through introduction of wild type or mutant genes (XYL1/XYL1 (R276H), XYL2, and XYL3) coding for xylose metabolic enzymes from Scheffersomyces stipitis. The resulting engineered strains, however, often yielded undesirable phenotypes such as slow xylose...

journal_title：Journal of biotechnology

authors： Kim SR,Lee KS,Kong II,Lesmana A,Lee WH,Seo JH,Kweon DH,Jin YS

更新日期：2013-03-10 00:00:00

abstract：:The genus Enterococcus occurs as native microbiota of fermented products due to its broad environmental distribution and its resistance to salt concentrations. Enterococcus faecalis F, a non-pathogenic strain isolated from a ripened cheese, has demonstrated useful enzymatic capabilities, a probiotic behavior and antib...

journal_title：Journal of biotechnology

authors： Serrano-Maldonado CE,Quirasco M

更新日期：2018-10-10 00:00:00

abstract：:As the understanding of variation is the key to a good process and product quality one should pay attention to dynamics on the single-cell level. The basic idea of this approach was to qualify and quantify variations on the single-cell level during bioreactor cultivations by monitoring the expression of an eGFP tagged...

journal_title：Journal of biotechnology

authors： Broger T,Odermatt RP,Huber P,Sonnleitner B

更新日期：2011-07-20 00:00:00

abstract：:During tumour development, macrophages are recruited to the tumour site and orientated towards an anti-inflammatory phenotype. Due to their immunosuppressive function, tumour associated macrophages (TAMs) are recognized as major components in tumour progression. Changing these macrophages to a pro-inflammatory phenoty...

journal_title：Journal of biotechnology

authors： Rodet F,Capuz A,Hara T,van Meel R,Duhamel M,Rose M,Raffo-Romero A,Fournier I,Salzet M

更新日期：2018-09-20 00:00:00

abstract：:4-vinylguaiacol, a kind of volatile phenolic compound with tobacco flavor, is widely used as a component of edible flavor and intermediate of medicine. Ferulic acid is usually used as substrate for the biosynthesis of 4-vinylguaiacol. However, the price of ferulic acid is high, leading to high production cost. In this...

journal_title：Journal of biotechnology

authors： Sun LH,Lv SW,Yu F,Li SN,He LY

更新日期：2018-09-10 00:00:00

abstract：:Amatoxins are ribosomally synthesized and post-translationally modified peptides (RiPPs) found in poisonous mushrooms. These cyclic peptides are potent inhibitors of RNA polymerase II transcriptional activity. Though the macrocyclization of amatoxin is extensively studied, little is known about its subsequent post-tra...

journal_title：Journal of biotechnology

authors： Pathiraja D,Byun J,Lee S,Choi IG

更新日期：2020-01-20 00:00:00

abstract：:Physiologically relevant in vitro models can serve as biological analytical platforms for testing novel treatments and drug delivery systems. We describe the first steps in the development of a 3D human brain tumour co-culture model that includes the interplay between normal and tumour tissue along with nutrient gradi...

journal_title：Journal of biotechnology

authors： Ivanov DP,Parker TL,Walker DA,Alexander C,Ashford MB,Gellert PR,Garnett MC

更新日期：2015-07-10 00:00:00

abstract：:A fibrous-bed bioreactor containing the coculture of Pseudomonas putida and P. fluorescens immobilized in a fibrous matrix was developed to degrade benzene (B), toluene (T), ethylbenzene (E), and o-xylene (X) in synthetic waste streams. The kinetics of BTEX biodegradation by immobilized cells adapted in the fibrous-be...

journal_title：Journal of biotechnology

authors： Shim H,Yang ST

更新日期：1999-01-22 00:00:00

abstract：:Gene prediction methods for eukaryotic genomes still are not fully satisfying. One way to improve gene prediction accuracy, proven to be relevant for prokaryotes, is to consider more than one model of genes. Thus, we used our classification of Arabidopsis thaliana genes in two classes (CU(1) and CU(2)), previously del...

journal_title：Journal of biotechnology

authors： Mathé C,Déhais P,Pavy N,Rombauts S,Van Montagu M,Rouzé P

更新日期：2000-03-31 00:00:00

abstract：:Phycocyanin (PC) prepared from a cyanobacterium Spirulina platensis by the DEAE-DE52 cellulose column chromatography that was developed by gradient elution of 50-250 mM phosphate buffer (pH 7.0) was stabilized by its subunits cross-linked covalently with formaldehyde. The single blue band that the chemically stabilize...

journal_title：Journal of biotechnology

authors： Sun L,Wang S,Qiao Z

更新日期：2006-02-24 00:00:00

abstract：:Pedobacter cryoconitis PAMC 27485, an aerobic, Gram-negative, facultatively psychrophilic bacterium, was isolated from Antarctic soil. Here we report the complete genome of P. cryoconitis PAMC 27485, which contains a type II CRISPR-Cas system and genes encoding useful enzymes (e.g. proteases). The genome sequence of P...

journal_title：Journal of biotechnology

authors： Lee J,Jung YJ,Lee HK,Hong SG,Kim OS

更新日期：2016-05-20 00:00:00

abstract：:A common approach for analyzing large-scale molecular data is to cluster objects sharing similar characteristics. This assumes that genes with highly similar expression profiles are likely participating in a common molecular process. Biological systems are extremely complex and challenging to understand, with proteins...

journal_title：Journal of biotechnology

authors： Ulfenborg B,Karlsson A,Riveiro M,Andersson CX,Sartipy P,Synnergren J

更新日期：2021-01-20 00:00:00

abstract：:A repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a Rhodococcus mutant. The addition of glutamate, thiamine, and MgSO(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance ...

journal_title：Journal of biotechnology

authors： Koike K,Takeuchi K,Mino H,Takaiwa M,Tohoh T,Tadokoro T,Tsutoh K,Ito S

更新日期：2000-06-23 00:00:00

abstract：:Previous research showed that co-down-regulation of caspase-3/7 in rCHO cells, unlike Bcl-2 overexpression, did not effectively block apoptotic cell death induced by 3mM sodium butyrate (NaBu). Here, it is found that the control of autophagy is also related to this different response to NaBu treatment. With NaBu treat...

journal_title：Journal of biotechnology

authors： Lee JS,Kim YJ,Kim CL,Lee GM

更新日期：2012-09-15 00:00:00

abstract：:The effects of a deletion of the amtR gene, encoding the master regulator of nitrogen control in Corynebacterium glutamicum, were investigated by metabolome and transcriptome analyses. Compared to the wild type, different metabolite patterns were observed in respect to glycolysis, pentose phosphate pathway, citric aci...

journal_title：Journal of biotechnology

authors： Buchinger S,Strösser J,Rehm N,Hänssler E,Hans S,Bathe B,Schomburg D,Krämer R,Burkovski A

更新日期：2009-03-10 00:00:00

abstract：:NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich d...

journal_title：Journal of biotechnology

authors： Noda T,Kawamura R,Funabashi H,Mie M,Kobatake E

更新日期：2006-11-01 00:00:00

abstract：:The spatiotemporal analysis of bioregulatory mechanisms at the level of intracellular multienzyme complexes and organelle interactions is made possible by the availability of endogenous and exogenous fluorescence probes, the development of microspectrofluorometers allowing one- and two-dimensional scans of intracellul...

journal_title：Journal of biotechnology

authors： Kohen E,Kohen C,Prince J,Schachtschabel DO,Hirschberg JG,Morliere P,Santus R,Dubertret L,Shapiro BL

更新日期：1990-01-01 00:00:00

abstract：:The circadian clock schedules processes in microalgae cells at suitable times in the day/night cycle. To gain knowledge about these biological time schedules, Neochloris oleoabundans was grown under constant light conditions and nitrogen limitation. Under these constant conditions, the only variable was the circadian ...

journal_title：Journal of biotechnology

authors： de Winter L,Schepers LW,Cuaresma M,Barbosa MJ,Martens DE,Wijffels RH

更新日期：2014-10-10 00:00:00