Abstract:
:Acetogenic bacteria like Acetobacterium woodii represent an ancient group of anaerobic microorganisms which use hydrogen and carbon dioxide to produce acetate. Cell concentrations and space-time yields are usually low in gas fermentations. A standard stirred‑tank bioreactor with continuous gas supply was equipped with a customized submerged microfiltration unit. A. woodii showed similar growth behavior with an initial maximal growth rate of 1.2 d(-1) in continuous gas fermentations with full cell retention and varying dilution rates. A steady increase of cell mass concentrations was observed with the highest biomass formation at the highest dilution rate. By contrast the final acetate concentrations were lowest at the highest dilution rate. The highest final acetate space-time yield of 148 g l(-1) d(-1) was measured at the highest dilution rate (increase by factor 8 compared to a standard batch process or by factor 37 compared to published data). The highest reported cell concentration of A. woodii in gas fermentations of nearly 14 g l(-1) cell dry weight was achieved in the submerged membrane bioreactor with increased yeast extract concentrations in the feed medium. Product inhibition was observed when acetate concentrations exceeded 8-12 g l(-1) causing a steady decrease in cell mass specific acetate production rates.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Kantzow C,Mayer A,Weuster-Botz Ddoi
10.1016/j.jbiotec.2015.07.020subject
Has Abstractpub_date
2015-10-20 00:00:00pages
11-8eissn
0168-1656issn
1873-4863pii
S0168-1656(15)30077-8journal_volume
212pub_type
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